This thesis investigated the cooperation of the Kras2 oncogene with the tumor suppressor gene Dlc1 in lung tumor development. Dlc1 is a negative regulator of RhoGTPase proteins, which are mainly involved in the regulation of the actin cytoskeleton and cell migration. We hypothesized that loss of Dlc1 expression leads to more aggressive tumors, which should also result in increased incidence of metastasis. All experiments were performed in mice containing a heterozygous oncogenic Kras allele and a heterozygous gene trapped Dlc1 allele (KD) and in mice only carrying the oncogenic Kras allele (K+). Throughout all experiments we have consistently found no significant differences between the two groups in terms of tumor burden (tumor numbers, sizes and areas), metastases or methylation patterns. These results suggest that heterozygous downregulation of Dlc1 is not enough to increase tumor formation and metastasis development in the Kras lung tumors.

Pancreatic ductal adenocarcinoma (PDAC) is a cancer of unmet need with a 5-year survival following diagnosis of 3% with limited surgical, radiotherapy and chemotherapy treatment options. Central to PDAC tumorigenesis is acquisition of an oncogenic Kras mutation to drive acinar-to-ductal metaplasia and progression to PDAC that is potentiated by NF-kB deregulation. However, PDAC requires the additional loss of tumour suppressors such as p53, SMAD4 or p16. ASPP family members ASPP2 and iASPP regulate both p53 and NF-kB, and are classified as a tumour suppressor and oncogene respectively. However, the precise roles of ASPP2 and iASPP in pancreatic cancer are unknown. In this thesis I demonstrate that ASPP2 suppresses metastasis and iASPP suppresses the pro-inflammatory tumour microenvironment. In a mouse model of PDAC development, ASPP2-deficiency does not alter metaplasia, PanIN progression or primary PDAC onset. However, median survival due to metastasis is significantly reduced in an ASPP2-deficient PDAC model. I demonstrate ASPP2-deficient PDAC can result in increased squamous differentiation defined histologically or via increased p63 expression. I propose ASPP2 is a key suppressor ΔNp63 and the squamous PDAC subtype in vivo. Conversely, iASPP is a putative oncogene and high expression in cancer associates with poor prognosis. However, in a mouse model of PDAC, loss of iASPP accelerates PDAC onset and metastasis. I demonstrate that iASPP is a functional tumour suppressor of a pro-inflammatory phenotype in response to oncogenic Kras and pancreatitis. I propose ASPP2- and iASPP-deficient mouse models of PDAC represent in vivo the squamous and immunogenic subtypes of PDAC respectively; and are relevant tools to study mechanisms of metastasis and inflammation-driven carcinogenesis.

This thesis is focused on the Rudice ponds problematique. Rudice ponds are small water bodies that were created as a result of mining activities and are unique biotope in the Moravian Karst region. The thesis contains overview of available literature on the subject of Rudice ponds, their brief history and general area relations of the Moravian Karst and the Rudice plateau. The main subject of this thesis is mapping and registering Rudice ponds using GNSS receiver. Card with gathered information has been created for each of Rudice ponds together with photodocumentation and will be used by the administration of protected landscape area Moravian Karst. The final part of the thesis is aimed at suggesting management of the Rudice ponds.

This thesis deals with the content of soil organic matter and soil reaction to selected locations in protected landscape area Moravian karst. 30 lokalities was compared (10 arable land, 10 permanent grassland, 10 forest). Samples were taken from surface mineral horizon in 2011 and 2013. In the theoretical part is discussed the issue of soil organic matter and soil reaction. There is characterized area Moravian Karst. The practical part includes processing methods, results, evaluation, statistical and graphical processing. Average content of humus increases in the order: arable land < permanent grassland < forest. The values in 2013 were lower than in 2011. Soil reaction was in 2011 for arable land, permanent grassland and forest vegetation very similar. Soil reaction was in 2013 similar only with arable land and grassland, the forests were lower. The values in 2013 were lower than in 2011 In 2013, the soil reaction is similar only with cropland and grassland, the forests were lower. Based on analysis of variance (ANOVA: one factor) was shown influence the type of vegetation on humus content. Influence the type of vegetation on soil reaction wasn't shown. A statistically significant difference was demonstrated between the measured values of cnotent humus and soil reaction in 2011 and 2013.

Najtová, S.: The analysis of geocaching at PLA Moravian Karst. Diploma thesis. Brno, 2015. The thesis describes the geocaching as a tool and an additional tourism activity. The theoretical part defines the basic principles of the game, rules, types of geocaches, player equipment and from which views can be seen on geocaching. Theoretical part also includes history in the Czech Republic and abroad too. The research was carried out on the territory of the PLA Moravian Karst. The thesis analyzes the geocaching in the selected location and focuses for example on the development of "caches", attendance, login or the difficulty of the terrain or caches searching. We can not also forget that it is a protected landscape area, therefore, are examined negative impacts of geocaching on the environment. The gained knowledge can be used used to estimate the future development of geocaching in the Moravian Karst and also prevent negative impacts on the environment.

Šárka Mazalová: Analysis and resolution of conflicts between recreation and conservation in Moravian Karst PLA This thesis is focused on the analysis of the impacts of recreation and tourism on conservation in Moravian Karts PLA and description of the major conflicts. The first part is a literature review related the general definition recreation, tourism and approach to the assessment of recreation conflicts are defined too. Another part is devoted to a description of the specific study area, including identification of priorities for recreation and tourism and significant phenomena of nature conservation. The thesis describes the largest leisure activities and their impact on nature conservation. The practical part of the work reported major conflicts between recreation and nature conservation with respect to the load space due to high number of visitors.

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