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Species identification of Klamath Basin suckers (Pisces : Catostomidae) and an assessment of hybridization using anonymous nuclear lociWagman, David Wolfe 12 November 2003 (has links)
Low copy number anonymous nuclear loci were used to search for species
markers in four species of Klamath Basin suckers. We sequenced 28 randomly
chosen loci representing 10,421 bp; 21 loci were similar to sequences in GenBank.
Eight fixed sequence differences were found among Klamath species. Locus 120
contained rare but diagnostic markers for Deltistes luxatus and for Catostomus
rimiculus. Locus 4 also contained three rare but unique sites in Catostomus
rimiculus. No sequence differences were found between Chasmistes brevirostris and
Catostomus snyderi. Loci 4 and 120 exhibited allele frequency differences between
Rogue River C. rimiculus and all Klamath Basin suckers. Genotype BB of locus 4
was a fixed diagnostic marker and genotype BB of locus 120 was a frequency
dependent marker for Rogue C. rimiculus.
Although Klamath suckers represent three genera, very limited variation was
found among 10,431 base pairs. We examined phylogenetic patterns of five loci in
eleven catostomid genera and 25 species to determine if the homogeneity in the
Upper Klamath Basin was due to massive hybridization and introgression or to
retention of ancestral sequences. Two loci with no similarity to GenBank sequences
(non-coding loci) and three loci with substantial similarity to GenBank sequences
(coding loci) gave similar results, providing support for various subfamilies and
tribes, more support for eastern genera and little support for western genera. Each
locus was a mosaic of species or population markers, sometimes providing
discriminatory power for allopatric populations of a species, such as C. macrocheilus,
while not discriminating other species. Upper Klamath Basin species were
noteworthy in their lack of autapomorphies, but had similar numbers of derived
informative sites as other catostomins. Upper Klamath Basin species consistently
shared ancestral or equivocal informative sites either with moxostomatins or a
variable group of western species and shared derived sites with other western species,
especially C. occidentalis. The data suggest that Upper Klamath Basin species have
retained a largely ancestral genome at these loci. Thus, the failure of this technique to
uncover significant variation in Upper Klamath Basin species may be a reflection of
their plesiomorphic genome at these loci and not necessarily hybridization. / Graduation date: 2004
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Contextualizing and Evaluating the Klamath Basin Restoration AgreementWilson, Jackman 01 January 2016 (has links)
This thesis explores the background of the Klamath Basin Restoration Agreement, a 250 page water use agreement among irrigators, Indian tribes, fishermen, environmental groups, federal and state agencies in the Klamath Basin. The agreement is contextualized in terms of water rights law, Indian law and the Endangered Species Act. The specific details of the agreement are explored. Finally, this thesis evaluates the agreement's merits and suggests a path forward for the agreement to become law.
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Influences on Stakeholder Participation in Water Negotiations: A Case Study from the Klamath BasinHorangic, Alexandra, Berry, Kate A., Wall, Tamara 15 March 2016 (has links)
In water governance, where problems are controversial and value laden, different forms of stakeholder involvement have become common and are frequently required. Stakeholder participation is often recognized as fundamental to the legitimacy and success of negotiated environmental decisions, but the intricacies of why stakeholders participate has received less attention. We examine factors that influenced stakeholder participation in the Klamath Basin Restoration Agreement and Klamath Hydroelectric Settlement Agreement of 2010. The research draws on in-depth, semistructured interviews of a sample recruited from stakeholder organizations in the Klamath River Basin. Results indicate that previous negative experiences did not translate into nonparticipation; divisions within seemingly aligned stakeholder organizations encouraged some stakeholders to participate and others to actively oppose negotiations; stakeholders' perceptions of power differentials encouraged both stakeholder participation and exclusion in negotiations; and concerns about relationship development during negotiations suggest that relationship building may be viewed as part of the negotiation process.
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Bioassessment of irrigation drainwater effects on aquatic resources in the Klamath Basin of California and Oregon /Bennett, Jewel Kay. January 1994 (has links)
Thesis (Ph. D.)--University of Washington, 1994. / Vita. Includes bibliographical references (leaves [134]-156).
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Transforming Regulatory Processes: Karuk Participation in the Klamath River Total Maximum Daily Load (TMDL) ProcessStoll, Shannan 21 November 2016 (has links)
This thesis examines the extent to which the Karuk Tribe has participated in natural resource management regulatory processes, using the Klamath River Total Maximum Daily Load (TMDL) process as a particular case study for evaluation. One of the most effective ways that the Tribe participates in the TMDL process is through the rigorous adoption of the technical tools of regulatory science. Collaboration with nontribal organizations is also used to build capacity for participation. The Tribe’s active participation in the TMDL process has in turn shaped the process, making it more inclusive of tribal values and traditional knowledge, improving overall scientific inquiry, and facilitating increased cooperation among tribal and non-tribal resource managers. At the same time, the Tribe’s participation in the process remains “uneven,” underscoring the ongoing challenge of making regulatory processes that recognize the legitimacy of tribal knowledge and values.
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Using Archaeological Fish Remains to Determine the Native Status of Anadromous Salmonids in the Upper Klamath Basin (Oregon, USA) Through mtDNA and Geochemical AnalysisStevenson, Alexander E. 01 January 2011 (has links)
Within the Upper Klamath Basin, Oregon, the native status of anadromous salmonids (Oncorhynchus spp.) has been a long standing question. Ongoing efforts to establish if these fish were native to the region prior to the construction of the Copco I Dam on the Klamath River (c.1917) have relied on sparse, contradictory and sometimes unreliable historic documentation and informant testimony. Current restoration projects with very high financial and social costs necessitate accurate and reliable data on salmonid species which once called the region home. Often, archaeolofaunal remains present a novel way to determine species present in an area prior to major habitat losses. This project analyzed fish remains from five previously excavated archaeological sites within the Upper Klamath Basin to determine which salmonid species were present prior to dam construction. A total of 5,859 fish remains were identified to at least taxonomic order using morphological distinctions. Site collections were dominated by those of catostomids (suckers) and cyprinids (minnows). Archaeological deposits at these sites dated as far back as approximately 7,500 BP but were primarily from the last 2,000 years. Only eighty-one salmonid remains were observed within the sites included in this project. The low frequency of salmonid remains in these sites may be the result of cultural and/or natural processes such as density mediated attrition and archaeological sampling. Of these 81 specimens, 38 were subjected to mtDNA analysis for species identification. Seven specimens did not yield DNA sufficient for species identification, six specimens were identified as O. tshawytscha (Chinook) and the remaining 25 specimens were identified as O. mykiss (steelhead or redband trout). Geochemical analysis was used to determine the life history of the fish represented by the remains within these collections. Strontium Calcium (Sr:Ca) ratios were measured on twenty-eight specimens. Three specimens were determined to be from freshwater resident fish and 25 were determined to be from anadromous fish. The specimens which were genetically identified as O. tshawytscha were all determined to be anadromous. Of the 18 specimens which were identified as O. mykiss and were subjected to geochemical analysis two were from freshwater resident fish and sixteen were from anadromous fish. Four samples were not characterized genetically but were subjected to geochemical analysis; three of these were determined to be from anadromous fish and one from a freshwater resident fish. Thus, the remains of anadromous O. mykiss and O. tshawytscha were identified in archaeological deposits predating construction of the Copco I dam in the Upper Klamath Basin While the genetic and geochemical analyses confirm the presence of skeletal remains from anadromous salmonids in the Upper Klamath Basin archaeological sites prior to dam construction these remains may, represent fish caught elsewhere and traded in. Two hypotheses address the introduction of these fish remains into pre-dam archaeological deposits, either they were traded/transported in from elsewhere (Trade/Transport Hypothesis) or they were caught locally (Local Catch Hypothesis). Expectations linked to each of these hypotheses were generated using ethnographic information from across the Pacific Northwest, including modern testimony from the Klamath Basin. Fish heads were often removed soon after capture in order to reduce spoilage of the rest of the fish. Thus, assemblages with many head parts are probably the result of local catch while those without head parts are probably the result of trade and/or transport. Two approaches were used to estimate the extent to which fish heads were deposited in sites. Basic proportions of cranial to post cranial remains from two sites provided a varied picture and did not readily support either the Local Catch or Trade/Transport hypotheses. Evaluation using scaled proportions based on frequency of skeletal elements within the body (Minimum Animal Units) show that four of the five assemblages were dominated by cranial remains and therefore suggest these fish were locally caught. Small samples sizes make it difficult to rigorously evaluate the hypotheses, though the dominance of cranial remains suggests salmonids were taken locally. Together these data indicate that anadromous O. tshawytscha and O. mykiss were taken from waters within the Upper Klamath Basin prior to the construction of Copco I. This study has provided accurate and reliable data, using a novel approach, on which restoration efforts in the region can rely for proper species reintroduction and habitat restoration efforts.
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Investigating the Link Between Surface Water and Groundwater in the Tule Lake Subbasin, Oregon and CaliforniaPischel, Esther Maria 13 August 2014 (has links)
Water allocation in the upper Klamath Basin of Oregon and California has been challenging. Irrigators have increasingly turned to groundwater to make up for surface water shortages because of shifts in allocation toward in-stream flows for Endangered Species Act listed fishes. The largest increase in groundwater pumping has been in and around the Bureau of Reclamation's Klamath Irrigation Project, which includes the Tule Lake subbasin in the southern part of the upper Klamath Basin. Previous groundwater flow model simulations indicate that water level declines from pumping may result in decreased flow to agricultural drains in the Tule Lake subbasin. Agricultural drains on the Klamath Project are an important source of water for downstream irrigators and for the Tule Lake and Lower Klamath Lake National Wildlife Refuges. To better assess the impact of increased pumping on drain flow and on the water balance of the groundwater system, flow data from agricultural drains were evaluated to investigate the changes that have taken place in groundwater discharge to drains since pumping volumes increased. Additionally, a fine-grid groundwater model of the Tule Lake subbasin was developed based on the existing regional flow model. The fine-grid model has sufficient vertical and horizontal resolution to simulate vertical head gradients, takes advantage of time-series data from 38 observation wells for model calibration, and allows agricultural drains to be more explicitly represented. Results of the drain flow analysis show that the groundwater discharge to agricultural drains has decreased by approximately 4000 hectare-meters from the 1997-2000 average discharge. Most of this decrease takes place in the northern and southeastern portions of the subbasin. Results of the groundwater model show that the initial source of water to wells is groundwater storage. By 2006, approximately 56% of the water from wells is sourced from agricultural drains.
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