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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Glucomannan-poly(N-vinyl pyrrolidinone) bicomponent hydrogels for wound healing

Shahbuddin, M., Bullock, A.J., MacNeil, S., Rimmer, Stephen January 2014 (has links)
No / Polysaccharides interact with cells in ways that can be conducive to wound healing. We have recently reported that konjac glucomannan (KGM) which is comprised of D-mannose and D-glucose linked by beta-1,4 glycosidic chains, stimulates fibroblast proliferation. The aim of this study was to produce a range of crosslinked KGMs and bicomponent KGM containing hydrogels and to examine their potential for wound healing. Two types of KGM hydrogel were synthesized, biodegradable from crosslinked KGM and non-biodegradable by forming semi-IPNs and graft-conetworks with a second synthetic component, poly(N-vinyl pyrrolidinone-co-poly(ethyleneglycol) diacrylate) (P(NVP-co-PEGDA)), which was produced by UV initiated radical polymerization. Crosslinked KGM was formed by bimolecular termination of macro-radicals formed by oxidation with Ce(IV). Semi-IPNs were formed by copolymerization of NVP and PEGDA in the presence of KGM and in the graft-conetworks the KGM was also crosslinked using the Ce(IV) procedure. The hydrogels had different swelling properties and differences could be observed in their chemical structure using C-13 solid state NMR, DSC and FTIR. Both forms were cytocompatible but only the graft-conetworks had the ability to stimulate fibroblast metabolic activity and to stimulate the migration of both fibroblasts and keratinocytes. In conclusion a form of KGM hydrogel has been produced that could benefit wound healing.
2

An investigation of the biology and chemistry of the Chinese medicinal plant, Amorphophallus konjac

Yee, Melinda Chua Fui January 2011 (has links)
Konjac glucomannan (KGM), the main biologically active constituent of konjac flour extracted from corms of Amorphophallus konjac (konjac), can be used to prepare functional foods and may also have potential as a pharmaceutical product to combat obesity. The current study employed three experimental approaches to study the biology and chemistry of konjac, namely (1) glasshouse experiments to study the morphogenesis, growth and productivity of konjac plants, (2) a histological and immunocytochemical investigation of the localisation and developmental regulation of the deposition and metabolism of KGM in developing corm tissues, and (3) a comparative study of methodologies for the extraction and analysis of KGM. The current data demonstrated a morphological and functional separation between the ventral and dorsal regions of corms. The ventral region appeared to function as a source during the initial period of shoot development, while the dorsal region appeared to operate as a sink after the development of mature canopy. Once the corm reached maturity, both an inflorescence and a leaf were produced within a single season. It has also been demonstrated that the age of the ‘mother’ corm is an important factor affecting the quality of offsets produced. An anti-mannan antiserum detected a temporally regulated pattern of mannan epitope production within glucomannan idioblasts in developing corm tissues, with increased expression as the corm approached maturity/dormancy. The current observations also suggest that the mobilization of KGM initiates at the periphery of the corm and proceeds inwards towards the centre of the corm. Compositional analysis showed that the purified konjac flour (PKF) produced using a modified extraction procedure contained 92% glucomannan, with a weight average molecular weight (Mw), polydispersity index (PDI) and degree of acetylation (DA) of 9.5 ± 0.6 x 105 gmol-1, 1.2 and 2.8 wt. %. These data, plus Fourier-transform infrared spectral (FTIR) and zero shear viscosity analyses of the extract (PKF) were all consistent with the literature. Comparison of three existing methodologies for the quantitative analysis of the KGM content, namely 3,5-dinitrosalicylic acid (3,5-DNS), phenol-sulphuric acid and enzymatic colorimetric assays; indicated that the 3,5-DNS colorimetric assay was the most reproducible and accurate method, with a linear correlation coefficient of 0.997 and recoveries between 97% and 103% across three spiking levels of starch. In summary, this study has provided a better understanding of aspects of the biology and cultivation of A. konjac and has also produced methodologies which can be used as the basis for an improved good laboratory practice (GLP) for the commercial extraction and analysis of this multifunctional natural polymer.
3

Impact du glucomannane de konjac sur les interactions composés volatils - amidon de pomme de terre dans un gel hydraté / Impact of konjac glucomannan on interactions aroma compound - potato starch in a hydrated gel

Lafarge, Céline 08 December 2016 (has links)
L’objectif de ce travail est de démontrer que la présence de glucomannane de konjac (KGM) dans une matrice d’amidon de pomme de terre permet d’accroître sa stabilité physique sans inhiber l’encapsulation moléculaire de composés d’arôme par l’amylose. Pour cette étude, les deux polyosides choisis sont issus de tubercules de plantes abondantes dans la nature.L’amidon est connu pour interagir avec des composés volatils, soit en les piégeant dans la zone amorphe, soit en formant des complexes d'inclusion. Ce phénomène est appelé encapsulation moléculaire. Cependant, les matrices amylacées à forte teneur en eau présentent une synérèse pouvant être néfaste sur la stabilité du piégeage des composés d’arôme dans le temps. Le KGM possède une capacité à former des solutions extrêmement visqueuses. L’ajout de KGM à faible concentration (0,2 %) à une suspension d’amidon (5 %) perturbe la gélatinisation de l’amidon, accélère la rétrogradation de l’amylose et ralentit la rétrogradation de l’amylopectine. Lors d’un vieillissement accéléré, la présence de KGM assure la stabilité de la suspension d’amidon. Dans une matrice amidon – KGM, l’encapsulation moléculaire du carvacrol par l’amylose a été mise en évidence. Les complexes formés sont de type V6III. Leur formation est dépendante des conditions expérimentales. L’utilisation du propylène glycol favorise la formation de complexes amylose carvacrol. Lors d’un vieillissement accéléré, le KGM assure la stabilité du piégeage du carvacrol.La matrice amidon de pomme de terre – KGM avec un ajout du carvacrol en fin de process présente la stabilité physique du gel et la stabilité du piégeage du carvacrol les plus optimales. / The objective of this study is to demonstrate that the presence of konjac glucomannan (KGM) in a potato starch matrix enhances its physical stability without inhibiting the molecular encapsulation of aroma compounds by amylose. For that purpose, the two selected polysaccharides are from plant tubers, abundant in nature.Starch is known to interact with volatile compounds either by trapping in amorphous phase or by forming inclusion complexes. This phenomenon is called molecular encapsulation. However, at high water content, these starchy matrices exhibit syneresis that can be harmful to the stability of the aroma compounds trapping over time. KGM has the ability to form highly viscous solutions. Our results show that the addition of KGM at low concentration (0.2 %) in starch dispersion (5 %) disrupts the gelatinisation of starch, accelerates the retrogradation of amylose and delays the one of amylopectin. During accelerate aging, the presence of KGM ensures stability of starch suspensions.In starch – KGM matrix, the molecular encapsulation of carvacrol by amylose has been demonstrated. The complexes of caravacrol – amylose are V6III type structure. Their establishment is dependent on experimental conditions. The use of propylene glycol as carrier solvent of carvacrol promotes the formation of complexes between amylose and carvacrol. During accelerate aging, the presence of KGM ensures the stability of carvacrol trapping.The potato starch – KGM matrix with an addition of carvacrol at the end of the process shows the best physical stability of the gel and the best carvacrol trapping.

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