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The Global ETD Search service is a free service for researchers
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 1 to 2 of 2 (0.103 seconds)Spelling suggestions: "subject:"detype amino acid transporter"" "subject:"botype amino acid transporter""
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Establishment and Characterization of Mammalian Cell Lines Stably Expressing Human L-Type Amino Acid TransportersMorimoto, Emiko, Kanai, Yoshikatsu, Do, Kyung Kim, Chairoungdua, Arthit, Hye, Won Choi, Wempe, Michael F., Anzai, Naohiko, Endou, Hitoshi 01 December 2008 (has links)
System L (SL), a basolateral amino acid transporter, transports large neutral amino acids (LNAAs) in a Na+-independent manner. Previously, we identified two isoforms of transporters: L-type amino acid transporter 1 (LAT1) and 2 (LAT2) and revealed their distinct substrate selectivity and transport properties. In this study, to establish more stable human LAT1 (hLAT1) and LAT2 (hLAT2) in vitro assay systems, we established mouse cell lines stably expressing hLAT1 (S2-LAT1) and hLAT2 (S2-LAT2). Real-time quantitative RT-PCR analysis revealed that S2-LAT1 and S2-LAT2 cells express hLAT1 and hLAT2 mRNAs at 20 - 1000-fold higher levels than those of endogenous mouse Lat1 and Lat2. S2-LAT1 and S2-LAT2 mediated [14C]L-leucine transport properties were measured and corresponded to results observed via Xenopus oocytes. Using these cells, the data demonstrate that hLAT1 and hLAT2 exhibit different characters in the acceptance of α-methyl amino acids and amino acid-related compounds with bulky side chains such as thyroid hormones and melphalan. S2-LAT1 and S2-LAT2 cells are expected to facilitate hLAT1 and hLAT2 substrate recognition research and contribute to drug development by providing an efficient assay system to screen for chemical compounds that interact with hLAT1 and hLAT2.
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Pharmakogenetik des Zytostatikums Melphalan: Charakterisierung des Membrantransportes / Pharmacogenetics of the cytostatic drug melphalan:Characterization of the membrane transportKühne, Annett 28 April 2008 (has links)
No description available.
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