MosA is an enzyme from Sinorhizobium meliloti L5-30, a beneficial soil bacterium. Initial investigation into this enzyme categorized it as a methyltransferase. Further investigation revealed that this was incorrect, and that MosA is actually a dihydrodipicolinate synthase, part of the N-acetylneuraminate lyase superfamily. One of the characteristics of enzyme superfamilies is their low sequence identity, but relatively high structural similarity. The structural investigation reported here confirms the high structural similarity between MosA and other superfamily members. <p>Investigation of MosA was carried out by means of x-ray crystallography. It was believed that detailed structural information may shed light into not only the enzymatic mechanism, but also the inhibition of MosA by lysine, the final product of the enzymatic pathway. Insight into enzyme mechanism and inhibition may ultimately prove useful in herbicide or insecticide development, as other dihydrodipicolinate synthases from harmful fungi, bacteria, or plants, make attractive targets for inhibition. Lysine is an essential amino acid for humans, meaning that there is no endogenous lysine production to block the use of these hypothetical inhibitors. Specific inhibitors based on crystal structures have proven to be effective in the past and hopefully, will continue to be useful in the future. <p>Here we report the structure of MosA, solved to 1.95 Å resolution with lysine 161 forming a Schiff-base adduct with pyruvate. This adduct is consistent with the currently accepted dihydrodipicolinate synthase enzyme mechanism.

MosA is an enzyme from Sinorhizobium meliloti L5-30, a beneficial soil bacterium. Initial investigation into this enzyme categorized it as a methyltransferase. Further investigation revealed that this was incorrect, and that MosA is actually a dihydrodipicolinate synthase, part of the N-acetylneuraminate lyase superfamily. One of the characteristics of enzyme superfamilies is their low sequence identity, but relatively high structural similarity. The structural investigation reported here confirms the high structural similarity between MosA and other superfamily members. <p>Investigation of MosA was carried out by means of x-ray crystallography. It was believed that detailed structural information may shed light into not only the enzymatic mechanism, but also the inhibition of MosA by lysine, the final product of the enzymatic pathway. Insight into enzyme mechanism and inhibition may ultimately prove useful in herbicide or insecticide development, as other dihydrodipicolinate synthases from harmful fungi, bacteria, or plants, make attractive targets for inhibition. Lysine is an essential amino acid for humans, meaning that there is no endogenous lysine production to block the use of these hypothetical inhibitors. Specific inhibitors based on crystal structures have proven to be effective in the past and hopefully, will continue to be useful in the future. <p>Here we report the structure of MosA, solved to 1.95 Å resolution with lysine 161 forming a Schiff-base adduct with pyruvate. This adduct is consistent with the currently accepted dihydrodipicolinate synthase enzyme mechanism.

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Active damage control is a new technique which mimics the self-repairing capability of biological beings and can be used to greatly increase the structural integrity and fatigue life. This thesis describes two approaches used in active damage control: direct stress cancellation and indirect stress cancellation. Direct stress cancellation is illustrated using an example of active crack damage control with embedded shape memory alloy actuators. Both experimental and theoretical investigations have been conducted to analyze the damage control mechanism. The approach of indirect stress cancellation is demonstrated with active fatigue damage control of adhesive joints. The damage and active control mechanisms of the adhesive joints have been experimentally and theoretically investigated. / Master of Science

This study considers the application of object oriented programming approach to develop a Windows based program for the design of reinforced concrete T -beam bridges in accordance with the newly adopted AASHTO LRFD Bridge Specification. The program can perform both the analysis and design of the deck and beam. The program is interactive in nature and it is possible to change all input data and design parameters during execution. A series of second-degree interpolating polynomial functions were developed for representing influence line coordinates for various load effects in the deck and beam subjected to moving loads. The analysis results using the program were compared with those obtained from the commercial programs. The design results obtained from the program were compared with example problems for several test structures. Both design and analysis comparisons gave satisfactory results. The successful performance of the program demonstrates the significant advantages of the object oriented programming. It was concluded that the benefits of object oriented programming approach make this a viable technique for the development of computer applications for structural engineering. / Master of Science

This thesis is a series of experiments performed on how ribbon-like bands of material can be folded to generate special volumes that can accommodate the functional aspects of a building program. / Master of Architecture

Master of Science

A partial-wave analysis of pion photoproduction data up to a photon lab energy of 1.8 GeV has been performed. Both energy-dependent and energy-independent solutions have been obtained. The energy-dependent parametrization incorporates the recently determined elastic pion nucleon scattering amplitudes in such a way as to satisfy unitarity and utilize the resonance structure contained in the pion nucleon elastic amplitudes. Starting from the energy-dependent solution, energy-independent partial-wave solutions are obtained at a set of energies from threshold to 1.8 GeV. The data base used in the analysis contains 11,911 data from the reactions. The predictions of our solution are compared with the experimental data and previous analyses. Suggestions are made for future experiments. A total of sixteen resonances exist in the energy range from threshold to 1.8 GeV. These resonance states are studied using our energy-independent solutions. Photon decay couplings to the sixteen resonances are extracted. These couplings are also compared with previous solutions and quark model predictions. / Ph. D.

In this thesis I describe experimental work and present data on the guiding of Rubidium atoms along free-space propagating light beams as well as within hollow core glass fibres, namely photonic crystal fibres. I describe experiments, laser systems and vacuum trap assemblies designed to facilitate this guiding. These experiments are intended to aid progression within the field of cold atom guidance wherein narrow diameter, long distance hollow-fibre guides are a current goal. Realisation of these guides could lead to promising applications such as atom interferometers and spatially accurate, multi-source, atom depositors. Herein, guided fluxes are observed in free-space guiding experiments for distances up to 50mm and up to 10GHz red-detuning from resonance. Additionally hollow-core, Kagome structured, quasi- and true-photonic crystal fibres are characterised. Finally a number of detailed fibre-guiding magneto-optic traps are developed. Both cold atomic-beams and cold atomic clouds are reliably positioned above fibre entrance facets in conjunction with a guiding laser beam coupled into the fibre core. Issues regarding optical flux detection outwith fibre confinement appear to have hindered observation of guided atoms. A far more sensitive detection system has been developed for use in current, ongoing fibre-guide experiments.

This thesis seeks to investigate the ecological theme in German-Swiss prose of the last thirty years. The role of nature has understandably always been significant in Swiss literature. In a nation that has eked out its living, in such an impressive and violent landscape, there is of necessity a highly developed awareness of the environment. Furthermore, the close relationship between mankind and the environment is inherently ambiguous, with each acting alternately as curse and blessing to the other. The bond between people and geography is made all the more vital in the Alps, where existence is under the constant threat of avalanches and landslides. In light of this heightened environmental sensibility, it is unsurprising that, with the growing profile of ecological debate in general, Swiss writers should demonstrate an acute cognisance of the significance of ecological problems. The notion of an ecological voice takes the discussion further. The question is posed whether these works merely represent a reflection of societal concern for the environment, or whether literary responses may constitute solutions. This investigation therefore contributes both to literary criticism on Swiss writing and to the understanding of the role of conceptualisation in finding solutions to ecological problems. To explore and analyse these ideas, this thesis considers a representatively broad spectrum of differing responses to ecological crisis. It is not intended to be an exhaustive list of recent Swiss ‘Öko-Literatur', but instead to be an investigation of the variety of narrative strategies employed in this period of growing ecological awareness.