Lead is a toxic heavy metal associated with adverse health effects ranging from developmental neurotoxicity to reproductive effects. While lead affects people of all ages, infants and children are the most vulnerable and susceptible to the neuro-developmental effects of lead exposure. Maternal blood lead concentrations that do not produce clinical toxicity on pregnant women have been linked to adverse offspring development. Observed reproductive effects to low lead levels during pregnancy include the risk of spontaneous abortions, effects on birth weight and preterm birth. There are particular concerns with regard to reductions in IQ scores. Research evidence suggests that an incremental increase in blood lead levels of 1 µg/dL is associated with approximately 1 IQ point deficit. Of particular concern is that currently no threshold has been observed or exists for developmental neurotoxicity to the chronic low lead exposures levels. While the developed countries have built evidence for lead exposure sources, have identified the most vulnerable groups to lead exposure, and have instituted control actions for lead exposure, it is not the case in developing countries such as Botswana. Currently, there is very little knowledge about the potential sources of lead exposure among different population groups not only in Botswana but also in most developing nations. There is also an evident limited knowledge on the behaviours and practices of different population groups that could potentially expose them to lead in developing countries. This thesis explores the following questions: i) Are there specific risk behaviours and practices peculiar to pregnant women in Botswana that could potentially expose them to lead? ii) What are the environmental lead concentration levels and their potential to expose pregnant women? iii)What are the blood lead concentrations at each stage of pregnancy and after delivery in Botswana and, iv) Can we use the information from these three questions to predict lead exposure levels during pregnancy and after delivery? v) Can we use the new information to a develop a policy dissemination brief to inform policy on lead exposure sources in Botswana, develop guidelines for health professionals for assessing and screening lead exposure levels during pregnancy and after delivery, develop an awareness leaflet for lead education? To address the specific risk behaviour and practices of pregnant women, a comprehensive validated risk assessment questionnaire was administered among 142 pregnant women during the first trimester of pregnancy (defined as 8-12 weeks) in four villages of different geographical settings and nomenclature (small/rural, major and semi urban). For purposes of this work the validation process involved obtaining information (from experts in the field and communities) relevant to the purposes of the study and to confirm that the tools employed for collection of data in all trimesters were suitable in terms of both construct and content. Data was collected between September 2009 and February 2010. To address potential environmental sources of lead exposure during pregnancy soil (n=28), water (n=28) and traditional cosmetic clay - letsoku (n=3) samples were collected in November 2010, February 2011 and May 2011 from the homes and in the vicinity of the study population to determine lead concentrations. To know baseline blood lead levels at each stage of pregnancy, blood samples were collected from September 2009 to February 2011 from pregnant women between weeks 8-12 (first trimester, n=137), 20-24 (second trimester n=126) and weeks 34-36 (third trimester n=106). Blood lead levels of women who completed the entire study from trimester on until after delivery (n=63) were then used to construct blood lead prediction models using statistical models. Pregnant women in the study area ingested non-food items such as soil, match sticks, pencil, chalk and animal feed such as bone meal (86%). Women applied used and unused car oils (in particular brake fluid) and other harmful substances for “treatment of skin conditions and for beautification purposes (74%). Older women (defined as 35 years in this study) were at a significantly higher risk to ingest soils (p<0.01). Mean (±SEM) lead concentrations in water exceeded the WHO drinking water quality standards nineteen fold (0.19±0.019 ppm (n=28) Major villages, had significantly higher Pb concentrations (p<0.05) in soils and water compared to small villages. Mean blood lead levels (±SEM) for the first, second and third trimesters were 1.96(±0.14)µg/dL, 2.49(±0.17) µg/dL, 2.66(±0.19) µg/dL respectively. Blood lead levels increases from the first to third trimester ranged from 1.6-5%. Blood lead concentrations significantly differed among locations (p<0.01). The highest concentrations were observed in women from smaller villages that were poorer (p<0.02). Pica, multiple risk behaviours/practices (engaging in two or more risk behaviours/practices), trimester of pregnancy, poor food supplementation and diet were predictors of blood lead levels ≥ 2µg/dL. There was a dose response relationship between supplement intake and an increase in blood lead levels. These findings suggest that pregnant women and their unborn babies could potentially be exposed to lead because of the environment in which they live, their economic status, lifestyle, behaviors and practices. Drinking water is a potential threat for lead exposure, not only among pregnant women, but other vulnerable groups such as infants and children. This study is the first in Botswana and one of the few in Africa to investigate lead exposure sources at each stage of pregnancy and after delivery. It is also the first to identify new potential lead exposure behaviors and practices such as the application of auto oils by pregnant women for treatment of skin diseases. The findings suggest the need to train health workers and equip them with the skills and knowledge to assess and screen women who could potentially be exposed to lead. Further, pregnant women need to be sensitized on potential lead exposure sources, to prevent lead poisoning. This study has been able to use the results to develop a policy brief for disseminating the results to decision makers, guidelines for utilization by health workers to screen lead exposure levels and an awareness leaflet for pregnant women. These have been validated and pretested at community and Government levels. / Thesis (PhD)--University of Pretoria, 2013. / School of Health Systems and Public Health (SHSPH) / Unrestricted

This research investigated the chemistries and mechanisms involved in lead-cement systems through the study of a larger number of lead compounds.

Lead neurotoxicity is considered a problem in young children and the long-term effects of lead exposure on them have yet to be determined. Studies have been completed to determine how lead exposure has affected children through their most important developmental stages. However, there is a lack of research to uncover any long-term effects lead may have as children enter adulthood. The purpose of this study was to determine long-term effects of lead exposure on IQ. The theoretical foundation for this study is the Cattell Horn Carroll theory of intelligence, which is based on fluid (novel experiences) and crystallized (previous experiences) intelligence. This study focused on individuals who were exposed to lead as children and are now young adults (ages 18-25). These individuals were part of a class action lawsuit and referred to a clinical psychologist from a private clinic who conducted IQ testing. In this non-experimental quantitative study, a multiple regression analysis was conducted on secondary data. Independent variables were first and last blood lead and Appearance, Pulse, Grimace, Activity, and Respiration scores as indicated from medical records. The dependent variables were the perceptual reasoning index, and verbal comprehension index of the WAIS-IV administered by a licensed clinical psychologist. The results of this study showed that childhood lead toxicity did not predict IQ scores in young adulthood. More research needs to be completed so that governmental agencies will have more information to be proactive in creating and changing policies around the use of lead in products that people use daily. This could lead to positive social change by placing an emphasis on early identification and treatment of lead exposure.

Inorganic pesticides including natural chemicals such as arsenic, copper, lead, and sulfur have been used extensively to control pests in agriculture. Lead arsenate (PbHAsO4) was first used in apple orchards in the late 1890's to combat the codling moth, Cydia pomonella (Linnaeus). The affordable and persistent pesticide was applied in ever increasing amounts for the next half century. The persistence in the environment in addition to the heavy applications during the early 1900's may have led to many of the current and former orchards in this country being contaminated. In this study, soil samples were taken from several apple orchards across the state, ranging from Southwest to Northern Virginia and were analyzed for arsenic and lead. Based on naturally occurring background levels and standards set by other states, two orchards sampled in this study were found to have very high levels of arsenic and lead in the soil, Snead Farm and Mint Spring Recreational Park. Average arsenic levels at Mint Spring Recreational Park and Snead Farm were found to be 65.2 ppm and 107.6 ppm, respectively. Average lead levels were found to be 354.5 ppm and 442.3 ppm, respectively. Based on these results, Virginia needs to look at setting standards for lead and arsenic in soil to determine if cleanup of former agricultural lands will be necessary. / Master of Science

No description available.

No description available.

There is emerging concern about the potential for elevated lead in water after water utilities conduct EPA mandated (or voluntary) partial replacements of existing lead service lines. Connections between dissimilar metals results in the accelerated corrosion of the less noble metal via galvanic attack, increasing metal concentrations in water and posing potential public health risks. Many practical problems associated with stopping galvanic attack between copper:galvanized iron and copper:lead via use of dielectrics have also been raised. Galvanic corrosion can be effectively stopped by isolating the dissimilar metals; however, completely eliminating electrical continuity may not always be practical or allowed by code. Instead, increasing separation distance between the two metals was hypothesized to considerably reduce galvanic corrosion. Galvanic corrosion and lead leaching were evaluated for lead:copper connections with varying separation distances while maintaining electrical continuity. Increased distance between lead and copper pipe dramatically reduced the galvanic current and the magnitude of lead release. Galvanized iron and copper connections were also investigated using various commercial fittings, and results verified that a controlling factor was separation distance between the two dissimilar metals. When considering the long-term behavior of partially replaced lead service lines, detrimental effects from galvanic corrosion worsened with time. Even when water was sampled consistently at moderate flow rate, the condition representing traditional partial service line replacement was 40% worse than a full lead service line. At elevated flowrates, lead concentrations and variability increased for partly replaced lead pipe versus full lead pipe due to reservoirs of lead rust formed via galvanic corrosion. At low flowrates, these negative impacts were not observed. Finally, crevices formed by the use of commercial couplings increased lead release. Overall, the results enhance practical understanding of galvanic corrosion impacts and use of dielectrics in water service lines and premise plumbing. / Master of Science

No description available.

The relationship between some mechanical properties (tensile, creep and thermal fatigue behaviour) and microstructure has been investigated for lead sheet containing <0.06 wt % Cu (the eutectic composition), made either by a conventional milling (rolling) process, or by continuous casting (Direct Method). Milled lead sheet exhibits a recrystallised microstructure, the grain size of which decreases with increasing copper content; copper is present as particles of ≃1-5 μm long, formed from the initial needles or plates of the cast billet by spheroidisation, or by particle break-up during rolling. A comparison of materials produced by several manufacturers indicates that the copper distribution and final grain size are dependent on the thermo-mechanical history of the sheet. Direct Method (DM) sheet exhibits a cast cellular structure within grains which usually extend through the full thickness of the cast sheet; copper is present as a fine dispersion (particles ≃0.5 μm diameter) at cell boundaries. The copper distribution in both materials is stable to prolonged heating (100 hours at 200<SUP>o</SUP>C), but some grain growth occurred in the milled sheet. It was not possible to obtain a fully dispersed eutectic microstructure at the eutectic composition; primary lead dendrites (or cells) were always present. This is thought to be due to the difficulty of nucleating a copper particle at the very low copper concentrations used in this work. The tensile behaviour of specimens was investigated at various strain rates and temperatures. DM sheet exhibits an increase in UTS both with increasing copper content at each strain rate, and with increasing strain rate for each copper content; no systematic variation of strain with copper content was observed. The UTS of milled lead sheet (at ambient temperature and slow strain rates) was a maximum at 0.02 - 0.03 % copper. A steady increase in UTS with increasing copper content was obtained at higher strain rates (2.67 min<SUP>-1</SUP> and above) and low temperatures (≃200 K) and indicated that a time and temperature dependent softening process is active at ambient temperature and slower strain rates (up to 1.33 min^-1) which is thought to be grain boundary sliding, although no evidence for this has been detected in the recrystallised microstructure. Values of the work-hardening coefficient (n) and the strain-rate sensitivity (m) were determined for milled and DM sheet, and were found in all cases to be high. DM sheet exhibits good creep resistance, which increases with increasing copper content, owing to the large grains and stable grain boundaries at ≃90' to the direction of stress. Milled lead sheet was less creep resistant, exhibiting maximum creep resistance at 0.03% copper; this is thought to be due to competing processes of strengthening by copper (which pins grain boundaries to some extent) and grain boundary sliding, which increases with the corresponding decrease in grain size. Thermal fatigue tests have been conducted externally, using lead flashing lengths fixed to an outside wall, and in the laboratory using specially developed apparatus. The development of the apparatus, specimen shape and test cycle length is described. Cracking is usually multiple and intergranular; examination of the fracture surface indicates a combination of intergranular fatigue, creep processes (cavitation) and regions of ductile failure. The use of acetate replicas to trace crack growth has shown the migration of grain boundaries to directions of high stress in milled lead, and in 0.01% copper DM sheet. Grain boundary migration was not observed in the more stable DM microstructures of higher copper content.

During their production and normal use, electronic packages experience large temperature excursions, leading to high thermo-mechanical stress gradients that cause fatigue failure of the solder joints. In order to prevent premature failure and prolong the fatigue life of solder joints, there is a pressing need for the characterization of the solder, especially lead-free solder, at the micro-level (joint size). The characterization and modeling of solder behavior at the appropriate scale is a major issue. However, direct measurement techniques are not applicable to characterize the deformation response of solder joints because of their micro scale dimensions. Therefore, a non-contact measurement technique utilizing a Scanning Electron Microscope (SEM) in conjunction with Digital Image Correlation (DIC) has been developed. Validation was achieved by performing a four-point bending test in both an in-house optical system with DIC and inside the SEM. This non-contact measurement technique was then used to extract the stress-strain response of the solder. Mechanical tests were performed on solder joints that were created using the same type of solder balls used in the electronic industry and were representative of normal joint scales. The SEM-DIC technique has been proven to be applicable for the determining the stress-strain response of solder material at the micro-scale.This study resulted in a validated material characterization technique specifically designed for micro-scale material response. One of the main contributions of this study is that the method is a lot simpler and cheaper, yet highly effective, compared to the previous methods. This technique is also readily applicable to the measurement of the stress-strain response of any micro-scale specimen, such as other metals, polymers, etc. Also, the measured displacement field by obtained by DIC can be used as the base for calculating the strain field on the surface of a specimen.