The goal of this project was to design a lens utilisinga glide symmetric periodic structure. A Lüneburg lens forantennas with a center frequency of 8 GHz has been designed.The structure was constructed using a unit cell in the shape ofan angular Bowen knot. This unit cell was then etched on themetallic surface of a parallel plate waveguide. Two symmetrycases for the unit cell were studied, the mirror symmetry andthe glide symmetry. Mirror symmetry is when the unit cell wasmirrored on the opposite metallic sheet, and glide symmetrywhen the cell was mirrored and shifted half a period. Theglide symmetric case was found to be less dispersive and moreisotropic. Further, we study the influence on the refractive indexwhen changing the geometry of the unit cell. A sufficient rangeof refractive index for realising the lens was found. These resultswere used to construct a full lens. Simulation of the complete lensshows the desired point to planar wave behavior of a Lüneburglens. In conclusion, the angular Bowen knot with glide symmetryhas the potential to construct a functioning Lüneburg lens. / Målet för detta projekt var att konstrueraen lins med glidsymmetriska periodiska strukturer. En Lüneburg-lins för antenner med en centerfrekvens på 8 GHz har de-signats. Strukturen byggdes med en enhetscell i formen avett sankthanskors etsat på en metallisk plattkondensator fylldmed ett dielektriskt substrat. Två symmetrifall betraktades,spegelsymmetri, då enhetscellen speglades på motstående me-tallskikt, och glidsymmetri där den även var förskjuten en halvperiod. Glidsymmetrin fanns ha mindre spridning och störreisotropi. Därtill undersöktes förändringarna i brytningsindex dåenhetscellens geometri förändrades. En tillräcklig räckvidd avbrytningsindex kunde uppnås. Dessa resultat användes för attkonstruera en fullständig lins. Simulering av den kompletta linsenvisade det önskade punkt till planvågbeteendet hos en Lüneburglins. Slutsatsen är att enhetsceller i formen av ett sankthanskorsmed glidsymmetri har potentialen att konstruera fungerandeLüneburg-linser. / Kandidatexjobb i elektroteknik 2020, KTH, Stockholm

<p>Epithelial-mesenchymal transition (EMT) is a pathological process leading to the formation of anterior subcapsular cataract (ASC). Mediated by transforming growth factor beta (TGFβ), EMT involves the transformation of the monolayer of lens epithelial cells (LECs) into spindle-shaped myofibroblasts, which manifest as plaques directly beneath the lens capsule. TGFβ-induced EMT leading to ASC has been associated with the upregulation of two specific matrix metalloproteinases (MMPs), MMP-2 and MMP-9. Having identified MMP-2 and MMP-9 as participants in the formation of cataracts, the specific roles of either of these MMPs have yet to be determined.</p> <p>The current study utilized MMP-2 and -9 knockout (KO) mice to determine their unique roles in TGFβ-induced EMT. First, adenoviral injection of active TGFβ1 into the anterior chamber of MMP-2 KO mice led to the formation of distinct αSMA-positive anterior subcapsular plaques, in contrast to treated MMP-9 KO eyes, which were resistant. Additionally, an <em>ex vivo </em>mouse LEC explant system was established in these KO lines. In the isolated lens epithelial explants, TGFβ triggered a transformation of LECs from a tightly packed cuboidal monolayer to an elongated mesenchymal phenotype. This process involved a disruption in epithelial cell contacts indicated by a loss of E-cadherin, and an acquisition of myofibroblast marker, αSMA. In the absence of MMP-2, TGFβ was still able to induce EMT with E-cadherin loss and concurrent αSMA expression. In contrast, LEC explants from MMP-9 KO mice treated with TGFβ did not acquire a characteristic spindle-like phenotype and showed substantially less αSMA expression. Results from both of these approaches were consistent; MMP-2, but not MMP-9, KO mice stimulated with TGFβ exhibited phenotypic changes typical of those described in ASC formation, namely a loss in cell attachments, multilayering of previously epithelial-like cells, and αSMA reactivity. Therefore, while MMP-2 is not necessary, MMP-9 is critical to TGFβ-induced EMT in LECs.</p> / Master of Science (MSc)

<p>Despite the long history of topical eye drops and their use in delivering therapeutic agents to the anterior of the eye, efficient sustained delivery continues to be an elusive goal. The robust and effective clearance mechanisms that the eye is endowed with are significant delivery challenges and result in short drug residence times and low ocular bioavailability. The work carried out in this thesis focused on developing, synthesizing and characterizing silicone hydrogels and evaluating their potential as drug eluting inserts for more effective delivery of ocular pharmaceuticals. The first strategy (Chapter 2) focused on incorporating a novel hydrogel additive, hyaluronic acid, to promote hydrogel-drug ionic interactions that can function to increase drug loading and subsequent release dosage. Hydrogels composed of a hydrophilic monomer, N,N-dimethlacrylamide (DMA) or 2-hydroxyethyl methacrylate (HEMA), and a hydrophobic monomer, methacryloxypropyltris(trimethylsiloxy)silane (TRIS), were used as model contact lenses. By combining ionic interactions with molecular imprinting techniques within a single hydrogel, it was shown that this can produce a compound effect on drug uptake and release. Although greater control over release dosage was achieved, there was limited capacity for these materials to delivery timolol for extended periods with drug release occurring rapidly over a period of 1-2 days. However, there were clear differences in the release duration from the p(DMA-<em>co</em>-TRIS) and p(HEMA-<em>co</em>-TRIS) hydrogel formulations. Therefore, the second study (Chapter 3) aimed to better understand the relationship between the hydrogel chemical composition and the resultant material properties on the drug release characteristics. A range of hydrogels were synthesized with varying hydrophilic and hydrophobic monomers, which were then characterized by their water content, transparency, optical haze and surface wettability. The previous generation materials were evolved by incorporating a modified siloxy methacrylate TRIS(OH), a methacrylated polydimethylsiloxane macromonomer (mPDMS) and a polymerizable silicone surfactant (ACR). The properties of the hydrogels were dramatically affected by the nature and relative contribution of hydrophobic and hydrophilic monomers. The release of dexamethasone (DEX), an anti-inflammatory medication, was shown to vary significantly depending on the hydrogel formulations; often displaying faster release in high water content materials and slow release in low water content hydrogels. The mechanism of diffusion for lipophilic DEX in these hydrogel systems appeared to be through the internal aqueous network channels within the bulk. Over the range of hydrogels formulations that were tested, the release from them varied from approximately seven days to greater than two weeks.</p> / Master of Applied Science (MASc)

Cataract surgery is currently the most common surgical procedure done in the world. However, within 5 years, approximately half of these patients will develop posterior capsule opacification (PCO). In cataract surgery, the biological lens is replaced with an intraocular lens (IOL). PCO is caused by migration and transformation of residual lens epithelial cells (LEC) that remain in the capsule following the surgery. LECs which have migrated to the posterior capsule within the first month of surgery are thought to be the major contributors to PCO since after this time, the capsule completely seals. A mathematical model has been developed in order to better understand the process of LEC migration during PCO. The model addresses the impact of substrate and substrate modification as well as the presence and absence of the growth factors transforming growth factor beta (TGF(beta)) and fibroblast growth factor (FGF2). It was developed from a first order rate of decay model taken from process control. If the cell speed is divided by the distance travelled by the cell up to the point of posterior capsule breach, the time for the LECs to breach the capsule posterior can be calculated. The model was tested with literature data and was able to predict the effects of cell speed on the presence of various extracellular matrix components and growth factors. It was determined that potentially modification with fibronectin may be useful for the prevention of PCO Preliminary experimental validation of the model was performed by modifying silicone substrates with various extracellular matrix derived peptides. Results demonstrate that peptide modified surfaces may be more resistant to EMT by increasing cell adhesion and decreasing cell migration. Therefore, this LEC migrational model will be a useful tool in the development of superior IOLs and materials. / Thesis / Master of Applied Science (MASc)

No description available.

A real time low energy spectral measurement of the neutrinos coming from the Sun will give us a greater understanding of energy production in the Sun, and the mechanisms of neutrino mixing. We will, for the first time, measure the solar neutrino spectrum for all solar neutrinos <2MeV in particular pp, Be and CNO neutrinos, be able to compare the solar photon derived energy luminosity (Lï §) to the solar neutrino derived energy luminosity (Lï ®) independent of any solar model, explore dark energy with respect to mass varying neutrinos, and explore CNO abundances in the Sun. These measurements require new technology in Indium loaded scintillators and large scale detector designs, namely increased spatial resolution through a novel scintillation lattice. I will present the advances we are making to these fields at Virginia Tech as well as neutrino science and the physics of the LENS detector. / Ph. D.

A 3- to 5-fold increase in Ca2+ accompanies cataract formation induced by selenite. The mechanism of selenite cataractogenesis involves calcium activation of calpain with subsequent proteolysis within the lens nucleus. This study was undertaken to investigate the biochemical mechanisms that lead to calcium accumulation in these circumstances. The components responsible for rat lens calcium regulation were defined by using either lens membrane vesicle preparations or intact lenses. Both Na+ gradient-dependent Ca2+ uptake and efflux occurred in lens membrane vesicles. Experiments with intact lenses showed that Na + ICa2 + exchange plays an important role in lens calcium regulation. ATP-dependent Ca2+ uptake and Ca2+ -dependent ATP hydrolytic activity have been characterized in lens membrane vesicles. Therefore, both Ca2+ -ATPase and Na + ICa2+ exchange participate in rat lens calcium regulation. Calcium accumulation in lenses treated by selenite may result from either increased influx (via non-selective cation channel), decreased efflux (via Ca2 +-ATPase and Na+ ICa 2+ exchange) or both. The selenite effects on the different components involved in lens calcium regulation were tested. / Ph. D.

The purpose of this study was to examine counselor's self- perceived multicultural counseling competency (MCC) between counselors working in rural, suburban and urban communities across the Commonwealth of Virginia. The study compared professional counselors' perceptions of their own multicultural counseling competence based on the counselor's geographic area of origin, current geographic practice setting, and counselors' intersections of identities to better understand counselor MCC as it relates to cultural diversity. Sample data was collected through professional counseling organizations in Virginia. Participants completed a demographic survey as well as the Multicultural Awareness Knowledge Skills Survey-Counselor Edition-Revised (MAKSS-CE-R) to measure self-perceived MCC. Results indicate that there was no difference in self-perceived MCC among professional counselors in Virginia based on their practice location or area of origin (i.e. hometown). However, counselor's identified race/ethnicity were predictor's of self-perceived MCC. Findings suggest that training programs may be providing adequate opportunities for counselors to develop MCC. Allowing innovative approaches through technology, consultation, and adherence to the ACA Code of Ethics (2014) could be sufficient in counselor MCC regardless of geographic practice location. / Doctor of Philosophy / The purpose of this study was to understand how counselors in Virginia perceived their cultural competence. The researcher examined how counselors rated their own cultural competence in relation to the geographic location in which they grew up, currently work, as well as general demographic characteristics, to better understand counselors’ competence when working with diverse peoples. Participants completed a demographic survey as well as the Multicultural Awareness Knowledge Skills Survey-Counselor Edition-Revised (MAKSS-CE-R), which measures counselor cultural competence. Results indicate that there was no difference in perceived cultural competence among professional counselors in Virginia based on the geographic area where they worked or grew up. However, counselors who identified as a racial/ethnic minority also perceived themselves as having greater cultural competency. Findings suggest that training programs may be providing adequate opportunities for counselors to develop cultural competence. Allowing creative approaches through technology, consultation, and implementation of counselor’s professional codes of ethics could be enough for counselors to feel competent when working with diverse peoples, regardless geographic practice location.

No / PURPOSE. To develop and validate a questionnaire for the measurement of the impact of contact lenses on quality of life (QoL): The Contact Lens Impact on Quality of Life (CLIQ) Questionnaire. METHODS. The questionnaire was developed and validated using conventional methods and Rasch analysis to assure content validity, repeatability, construct validity, and low respondent burden. Item identification and selection (647 items) were performed with an extensive literature review, professional advice, and lay focus groups. Item reduction used focus groups and data obtained from 161 subjects completing a 90-item pilot questionnaire. Validity and reliability, from data of 128 additional subjects, were assessed using Rasch analysis, intraclass correlation coefficient, and Bland-Altman limits of agreement. RESULTS. A 28-item CLIQ Questionnaire was developed and shown to have good validity and reliability by Rasch analysis statistics: real person separation, 2.02; model person separation, 2.17; reliability, 0.80; root mean square measurement error, 2.73; mean square ± SD infit, 1.01 ± 0.18; outfit, 1.01 ± 0.19. The items (mean score, 49.8 ± 4.9) were well targeted to the subjects (mean score, 51.2 ± 6.2) with a mean difference of 1.35 (scale range, 0-100) units. Test-retest intraclass correlation coefficient (0.86) and coefficient of repeatability (±8.00 units) demonstrated good repeatability. CONCLUSIONS. Rasch analysis and standard psychometric analyses demonstrated that the 28-item CLIQ Questionnaire is a valid and reliable measure of QoL in contact lens wearers. A scoring algorithm is provided for CLIQ Questionnaire users to convert raw scores into the Rasch analysis-derived linear person measures.

No / PURPOSE. The Activities of Daily Vision Scale (ADVS) has been extensively validated by traditional methodology. In the current study, Rasch analysis was used to explore further the validity of the ADVS and to determine whether improvements could be made. METHODS. Forty-three patients with cataract underwent visual acuity (VA) and contrast sensitivity (CS) testing and completed the ADVS. The data were Rasch analyzed and the value of response scale and item reduction explored. A shortened version and the original ADVS were tested for criterion validity by determining correlations with VA and CS. RESULTS. The ADVS data contained nonnormally distributed items and items with ceiling effects and empty response categories. Therefore, items benefited from shortening the response scale, the optimum length being three responses. There was poor targeting of item difficulty to patient ability, because many patients with cataract were sufficiently able that they had no difficulty with many activities. Items were eliminated if the task was too easy or did not fit with the overall concept of visual disability determined by the Rasch model. A reduced ADVS version was established that had adequate precision, equivalent criterion validity, and improved targeting of item difficulty to patient ability, but this version was still not ideal. CONCLUSIONS. Despite careful traditional validation, the ADVS data contained inadequacies exposed by Rasch analysis. Through Rasch scaling, particularly with response scale reduction, the ADVS can be improved, but additional questions seem to be needed to suit the more able, including patients undergoing second eye cataract surgery. There remains a need to develop Rasch-scaled measures of visual disability for use in ophthalmic outcomes research.