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Endophyte infected Tall fescue (Festuca arundinacea Schreb.) is the most dominant grass used for pasture in the Southeastern U.S. As a result, fescue toxicosis is a major concern. Producers need alternative forages for grazing cattle that do not have this negative aspect. The objective of this experiment was to determine the grazing behavior of cattle grazing Lakota (L) prairie grass (Bromus catharticus Vahl.), endophyte infected (E+), endophyte free (E-), and novel endophyte (Q) tall fescues. Angus-crossbred steers (279±8 kg) steers wore electronic behavior data recorders in four sampling periods, and direct visual appraisals of behavior were taken in five sampling periods during the months of May to September, 2004. Overall, during the visual appraisal phase steers grazing L spent most time (P<0.05) grazing while E+ spent the least time grazing. Overall, steers grazing E+ spent more time (P<0.05) idling than those on L, E-, or Q. Steers grazing E+ spent more time (P<0.05) standing than steers grazing Q. Steers grazing Q and E- spent more time (P<0.05) lying than those grazing E+. During the data recorder phase there were no significant differences between treatments for time spent grazing. Steers grazing E+ spent less time (P<0.05) lying and ruminating than steers grazing Q or L. Conversely, time spent standing and idling for steers grazing E+ was higher (P<0.05) than for steers grazing Q or L. These results indicate that L, E-, and Q may offer benefits to producers due to more time spent in productive activities during summer months. / Master of Science

In 1985, 24 bottom samples were collected in each of two slightly brackish,semi-permanent prairie wetlands (P1 and P4) with different hydroperiods. The main objective was to determine if hydroperiod affected seed pool characteristics. Additionally, 48 samples were collected in 1986 from wetland P1 to determine if seed bank composition changed annually without a change in mature vegetation. Seed bank composition was determined by placing soil samples in a greenhouse, then counting and identifying emerged seedlings. As a check against the seedling emergence method, seeds were separated and identified microscopically in one-third of the 1985 samples. Results indicated that the emergence method was an accurate technique for assessing seed pool composition. The wetlands did not differ in floristic composition (i.e., presence/absence) but did in species densities. The mean relative density of mudflat annuals in all seed pool samples was significantly greater in wetland P4 (82%) than in P1 (52%). A shorter hydroperiod in this wetland produces more frequent drawdowns and a greater input of mudflat annual seeds. Conversely, seeds of emergent species were more abundant in the seed bank of wetland Pl (48%) compared to P4 (17%). The former wetland has a longer hydroperiod and less frequent drawdowns, and thus, the primary seed input is from emergent plants. / Master of Science

Matua prairie grass has a potential to extend the grazing season in Virginia due to its higher early spring and fall production. However, little is known about the compatibility of Matua prairie grass with legumes or the effects of legumes on the yield and quality of Matua prairie grass/legume mixtures. An experiment was conducted in 1998 and 1999 to investigate the botanical composition, yield, and chemical composition of Matua prairie grass grown with legumes. Legume treatments consisting of ladino clover (Trifolium repens), red clover (Trifolium pratense), alfalfa (Medicago sativa), and annual lespedeza (Lespedeza stipulacea) were drilled into a Matua prairie grass stand. Nitrogen was applied once each fall at two treatment levels of 0 or 84 kg/ha. The experiment was arranged in a randomized split block design with four replications. Legume treatments had no effect on percentage Matua prairie grass or total dry matter yield in 1998. However, in 1999 the ladino clover and red clover treatments increased (P<0.05) total dry matter yield, but also resulted in a substantial decrease (P<0.05) in percentage Matua prairie grass. Nitrogen application in the fall of 1998 had a residual effect (P<0.05) on the percentage Matua prairie grass and yield in 1999. The highest response to nitrogen fertilization occurred in the harvest immediately after fertilization, in October of 1999, which resulted in the largest increase (P<0.05) in percentage Matua prairie grass and yield, and the greatest decrease (P<0.05) in percentage legumes. The legume and nitrogen treatments similarly influenced the chemical composition of the Matua prairie grass/legume mixed forage. Ladino clover, red clover, and alfalfa treatments generally improved forage quality as indicated by a decrease (P<0.05) in NDF, ADF, hemicellulose, and cellulose, and an increase (P<0.05) in CP and IVDMD. Nitrogen fertilization did not influence the chemical composition of the forages to the same extent as the legume treatments, as a decrease in fiber components and an increase in CP and IVDMD were observed due to nitrogen. Overall, alfalfa appeared to be most compatible with Matua prairie grass, and the incorporation of alfalfa into a Matua prairie grass stand resulted in some improvements in total dry matter yield and nutritive value of the forage, without the detrimental suppression of Matua prairie grass. / Master of Science

The objective of this research was to assess the potential effects of global warming on the hydrology and vegetation in semi-permanent wetlands located in the glaciated prairie region of North Dakota. As a means to that objective, a spatially-defined simulation model of the vegetation dynamics in these wetlands was constructed. A hydrologic component of the model estimated water levels based on precipitation, runoff, potential evaporation and transpiration. Amount and distribution of emergent cover and open water were modeled using a geographical information system. Vegetation response to changes in water level was based on seed bank composition, seedling recruitment, establishment and plant survivorship. Simulation results were compared to actual distributions from aerial photographs (1979-89). Results showed that the model was relatively good at calculating changes in water level for average years. Late-summer water levels were overestimated during dry years due to limitations in the Thornthwaite method of calculating potential evapotranspiration. In general, changes in the ratio of emergent cover to open water were accurately simulated. Tests of the model elucidated two areas that needed improvement. First, seedlings germinated too quickly on exposed mudflats in the model when drawdown occurred late in the season. The actual wetland had a thick mat of dried, submergent vegetation on top of the mudflats which impeded germination, which the model did not consider. Second, model conversions between open water and deep marsh vegetation were not always timed correctly. If water depth crossed a threshold value for a given period of time a cell would change its type. In reality, tolerance of emergents to deep water is more complex. A probability function with respect to time and water depth rather than a threshold value would better represent this relationship. The model was used to assess the potential effects of global warming on the cover cycle in one wetland. An 11-year simulation was run using a normal versus greenhouse climate. Although water level fluctuations still occurred, peak values were significantly lower in the warming scenario and the wetland dried in most years. Simulations also revealed a significant change in the vegetation, from a nearly balanced cover ratio to a completely closed basin with no open water areas. / Ph. D.

This paper deals with a study of the ranch lands in Cooke, Wise, Denton, and Montague Counties, which are located in the North Grand Prairie of Texas.

The goals of captive breeding programs for endangered species include preserving genetic diversity and avoiding inbreeding. Typically this is accomplished by minimizing population mean kinship; however, this approach becomes less effective when errors in the pedigree exist and may result in inbreeding depression, or reduced survival. Here, both pedigree- and DNA-based methods were used to assess inbreeding depression in the critically endangered Attwater’s prairie-chicken (Tympanuchus cupido attwateri). Less variation in the pedigree-based inbreeding coefficients and parental relatedness values were observed compared to DNA-based measures suggesting that errors exist in the pedigree. Further, chicks identified with high parental DNA-based relatedness exhibited decreased survival at both 14- and 50-days post-hatch. A similar pattern was observed in later life stages (> 50 days post-hatch) with birds released to the wild; however, the pattern varied depending on the time post-release. While DNA-based inbreeding coefficient was positively correlated with mortality to one month post-release, an opposite pattern was observed at nine months suggesting purging of deleterious alleles. I also investigated whether immunocompetence, or the ability to produce a normal immune response, was correlated with survival; however, no significant correlation was observed suggesting that inbreeding was a more important factor influencing survival. Pairing individuals for breeding by minimizing DNA-based parental relatedness values resulted in a significant increase in chick survival. This study highlights the importance of using DNA-based methods to avoid inbreeding depression when errors exist in the pedigree.

Identifying mechanisms that determine who lives and dies is the first step in developing successful restoration techniques for rare species and endangered habitats. We studied interactions that affect establishment of native plant forbs of conservation concern at the seedling stage to support the theoretical basis for restoration activities in Pacific Northwest prairies. Specifically, we tested the hypothesis that seedling establishment is controlled by 1) competition with or 2) facilitation by existing vegetation and that the interaction is mediated in part by environmental stress. We direct-seeded or planted vegetative plugs of Lupinus oreganus, Castilleja levisecta, Erigeron decumbens, Iris tenax and Sidalcea malviflora ssp. virgata into 20 plots with a range of community compositions in high-stress upland prairies at each of three sites. We counted seedlings and estimated cover of plant functional groups as well as litter, bare soil and disturbance then used linear regression to test for effects of these factors on seedling establishment. We found evidence of indirect facilitation of grass on seedling establishment in the first year: higher accumulations of leaf litter increased seedling numbers at two sites. In the second year, there was evidence of facilitation by live vegetation and litter on seedlings at one site, but no net effect of either competition or facilitation at the other two sites. Overall, we found more evidence for positive interactions than we did for competition. In particular, litter appeared to have a positive effect on seedling establishment of L. oreganus and S. malviflora ssp. virgata. This is contrary to the common perception that litter inhibits plant establishment but supports the theory that facilitation is more common in high stress sites; practitioners should consider seeding into leaf litter at some sites. To support a robust approach to conservation and reintroduction of species with dormant seed, we characterized dormancy types and developed germination protocols for S. malviflora ssp. virgata and I. tenax. S. malviflora ssp. virgata has physical dormancy and may have physiological dormancy. Scarification followed by four weeks of cold moist stratification was effective in initiating germination. I. tenax has morphophysiological dormancy which is overcome by four weeks of warm moist stratification followed by 6-12 weeks of cold stratification. We also conducted a meta-analysis of experiments that tested pre-sowing seed scarification of L. oreganus and conclude that breaking physical dormancy prior to direct seeding does not support higher establishment relative to unscarified seeds in this species. / Graduation date: 2012

As a result of rapid growth in technology, efforts to remove pollutants from the natural environment have not been able to keep pace with the increasing amount of waste materials and a growing population that further aggravates the situation. This has resulted in the transformation of lakes and ponds into pollution depots. A pilot study for monitoring certain selected trace elements entering and leaving YorkPrairie Creek Pond, situated inside the campus of Ball State University, was undertaken with the view to establishing whether or not this pond has been polluted, like many lakes and ponds, by metal corrosion, engine exhaust gases, and runoff from fertilizers and streets.This investigation involved sampling pond waters at two sites, viz., Influx surface and Effluent surface. Samples were collected once daily at the same time over a total time period of 23 days. Each sample was analyzed, in duplicate, for the heavy metal cations iron, lead, and cadmium, and for the inorganic anions nitrate, orthophosphate, and chloride. Metals were analyzed by atomic absorption spectrophotometry (A. A. S.), while titrimetric and cotorimetric procedures were utilized for the anion analyses.Results have indicated that iron and chloride are the most abundant of all analytes. Precipitation tends to Increase concentrations of analytes in general, possibly due to increased runoff and the lowering of the pH of these waters; leading to increased elemental release into solution. The relative responses of the analyzed ion levels in pond influx and effluent waters have been shown to be similar although their levels were significantly different.Analyte levels were found to be generally higher in effluent than in Influx waters; only chloride was found to depart from this general behavior. This finding led to the conclusion that the selected Ions, with the exception of chloride, tend not to accumulate in the waters of the pond but rather to escape with its effluent. This conclusion is subject to modification by future researchers, to whom the author has recommended a more expanded sampling program that would include the surface and sediment of the pond's interior since the nature of the results (especially for Pb and Cd) would seem to indicate not only that the pond does not exhibit thorough mixing of its constituents (homogeneity) but also the presence of an active remobilization process within its interior.

<p>Greater prairie-chickens (<i>Tympanuchus cupido pinnatus</i>; GPC) have declined throughout large areas in the eastern portion of their range. I used species distribution modeling to predict most appropriate areas of translocation of GPC in and around Kankakee Sands, a tallgrass prairie in northwest Indiana, USA. I used MaxEnt for modelling the predictions based on relevant environmental predictors along with occurrence points of 54 known lek sites. I created four models inspired by Hovick et al. (2015): Universal, Environmental, Anthropogenic-Landcover, and Anthropogenic-MODIS. The Universal, Environmental, and Anthropogenic-MODIS models possessed passable AUC scores with low omission error rates. However, only the Universal model performed better than the null model according to binomial testing. I created maps of all models with passing AUC scores along with an overlay map displaying the highest predictions across all passing models. MaxEnt predicted high relative likelihoods of occurrence for the entirety of Kankakee Sands and many areas in the nearby landscape, including the surrounding agricultural matrix. With implementation of some management suggestions and potential cooperation with local farmers, GPC translocation to the area appears plausible.</p> <p>Franklin’s ground squirrels (<i>Poliocitellus franklinii</i>; FGS) have declined throughout a large portion of the eastern periphery of their range. Because of this, The Nature Conservancy is interested in establishing a new population of these animals via translocation. The area of interest is tallgrass prairie in northwest Indiana, USA: Kankakee Sands and the surrounding landscape. Species distribution modelling can help identify areas that are suitable for translocation. I used MaxEnt, relevant environmental variables, and 44 known occurrence points to model the potential for translocation of FGS to Kankakee Sands and the surrounding area. I created four models inspired by Hovick et al. (2015): Universal, Environmental, Anthropogenic-Landcover, and Anthropogenic-MODIS. I created maps of models with passing AUC scores. The final map was an overlay map displaying the highest relative likelihood of occurrence predictions for the area in all passing models. Only the Universal and Anthropogenic-MODIS models had passable AUC scores. Both had acceptable omission error rates. However, none of the models performed better than the null model (p < 0.05). MaxEnt predicted that a few areas in and outside of Kankakee Sands possess high relative likelihoods of occurrence of FGS in both the Universal and Anthropogenic-MODIS models. However, MaxEnt predicted high relative likelihoods in the surrounding agricultural matrix in the Universal Model. FGS prefer to cross through agricultural areas via unmowed roadside instead of open fields (Duggan et al. 2011). Because of this, high predictions in agricultural matrices in the Universal model are irrelevant. High relative likelihood predictions for linear sections that are obviously roads are disregardable in the context of my modeling efforts. Because of my low sample size, none of the models are really reliable in predicting relative likelihoods of occurrence for this area. Despite high relative likelihood predictions, the appropriateness of a translocation effort to the area is inconclusive.</p>