An analysis of algal biomass measured by chlorophyll-a concentration in Lake Texoma was performed as a part of a monitoring program to develop baseline environmental data in order to detect the potential effects of engineered changes in chloride concentrations in the reservoir. This portion of the research project focused on two main research objectives. The first objective was evaluating the effect of sampling strategy on the ability to adequately reflect standing crop estimates and trends in algal biomass. Two sampling regimes utilizing replication of three versus ten samples were applied and then analyzed using a minimum detectable difference algorithm to determine the necessary magnitude of replication to represent the variation in the metric. Chlorophyll-a distribution was analyzed for zonation patterns expected in a river-run reservoir to establish the importance of representative sampling of river, transition and main lake zones of the reservoir for management decisions and trophic characterization.

Simultaneous size fractionation of plankton populations associated with NaH^14CO_3 and ^3H-glucose uptake was employed in eutrophic Lake Texoma (Texas and Oklahoma) and oligotrophic Flathead Lake (Montana). Autoradiography was utilized to determine the role of specific microorganisms in community metabolism. Ultraplankton (0.45-10 μm) dominated plankton numbers and metabolic activity in both aquatic systems. Many of the most abundant species were not the most productive, in terms of inorganic C fixation. Rates of heterotrophic uptake of ^3H-glucose were small in comparison to photolithotrophic uptake in both lakes, Photoheterotrophy was more extensive in Flathead Lake, Autoradiographs indicated that bacteria were responsible for observed photoheterotrophy. Oscillatoria sp. exhibited. mixotrophy in Lake Texoma,