Flow past circular cylinders with stepwise discontinuities in diameter was investigated experimentally and numerically for the diameter ratio D/d = 2 and three Reynolds numbers, Re = 150, 300, and 1050. The investigation was focused on the vortex shedding phenomena occurring in the wake of the cylinders. In the first series of experimental and numerical studies, the flow development past a single step cylinder was investigated. The single step cylinder model is comprised of a small diameter cylinder (d) attached coaxially to a large diameter cylinder (D). The results show that three distinct spanwise vortex cells form in the step cylinder wake: a single vortex shedding cell in the wake of the small cylinder (the S-cell) and two vortex shedding cells in the wake of the large cylinder, one in the region downstream of the step (the N-cell) and the other away from the step (the L-cell). Due to the differences in vortex shedding frequencies between the three cells, complex vortex connections occur in two vortex-interaction regions located between the adjacent cells. The region at the boundary between the S-cell and the N-cell is relatively narrow and its spanwise extent does not fluctuate significantly. In this region, vortex dislocations manifested as half-loop connections between two S-cell vortices of opposite sign. In contrast, the region at the boundary between the N-cell and the L-cell exhibits a transient behavior, with large scale vortex dislocations causing cyclic variation in the extent of N-cell vortices. For Re = 300 and 1050, small scale streamwise vortices forming in the wake complicate the vortex dynamics within the adjacent S-cell and L-cell. There is no significant Reynolds number effect on the average spanwise extent of the vortex cells and the two transition regions between neighboring cells. Finally, formation of N-cell vortices is linked to downwash fluctuations near the step. The flow development past a dual step cylinder was studied experimentally for Re = 1050. The dual step cylinder model is comprised of a small diameter cylinder (d) and a large diameter cylinder (D) mounted at the mid-span of the small cylinder. The experiments were completed for a range of large cylinder aspect ratios 0.2 ≤ L/D ≤ 17. The flow development is highly dependent on the aspect ratio of the large cylinder, L/D. The results identify four distinct flow regimes: (i) for L/D = 17, three vortex shedding cells form in the wake of the large cylinder, one central cell and two cells of lower frequency extending over about 4.5D from the large cylinder ends, (ii) for 7 < L/D ≤ 14, a single vortex shedding cell forms in the wake of the large cylinder, whose shedding frequency decreases with decreasing L/D, (iii) for 2 ≤ L/D ≤ 7, vortex shedding in the wake of the large cylinder is highly three-dimensional, with vortices deforming in the near wake, (iv) for 0.2 ≤ L/D ≤ 1, only small cylinder vortices are shed in the wake and can form vortex connections across the wake of the large cylinder.

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Tsunami are a fascinating but potentially devastating natural phenomena that have occurred regularly throughout history along New Zealand's shorelines, and around the world. With increasing population and the construction of infrastructure in coastal zones, the effect of these large waves has become a major concern. Many natural phenomena are capable of creating tsunami. Of particular concern is the underwater landslide-induced tsunami, due to the potentially short warning before waves reach the shore. The aims of this research are to generate a quality benchmark dataset suitable for comprehensive comparisons with numerical model results and to increase our understanding of the physical processes involved in tsunami generation. The two-dimensional experimental configuration is based on a benchmark configuration described in the scientific literature, consisting of a semi-elliptical prism sliding down a submerged 15° slope. A unique feature of these experiments is the method developed to measure water surface variation continuously in both space and time. Water levels are obtained using an optical technique based on laser induced fluorescence, which is shown to be comparable in accuracy and resolution to traditional electrical point wave gauges. In the experiments, the landslide density and initial submergence are varied and detailed measurements of wave heights, lengths, propagation speeds, and shore run-up are made. Particle tracking velocimetry is used to record the landslide kinematics and sub-surface water velocities. Particular attention is paid to maintaining a high level of test repeatability throughout the experimental process. The experimental results show that a region of high pressure ahead of the landslide forces up the water over the front half of the landslide to form the leading wave crest, which propagates ahead of the landslide. The accelerating fluid above, and the turbulent wake behind, the moving landslide create a region of low pressure, which draws down the water surface above the rear half of the landslide to form the leading trough. Differences in the phase and group velocities of the components in the wave packet cause waves to be continually generated on the trailing end of the wave train. The downstream position that these waves form continually moves downstream with time and the wave packet is found to be highly dispersive. The interaction of the landslide pressure field with the free surface wave pressure field is important, as the location of the low pressure around the landslide relative to the wave field acts to reinforce or suppress the waves above. This has a substantial effect on the increase or decrease in wave potential energy. When the low pressure acts to draw down a wave trough, the wave potential energy increases. When the low pressure is below a wave crest, it acts to suppress the crest amplitude, leading to an overall decrease in wave potential energy. Measurements of the efficiency of energy transfer from the landslide to the wave field show that the ratio of maximum wave potential energy to maximum landslide kinetic energy is between 0.028 and 0.138, and tends to increase for shallower initial landslide submergences and heavier specific gravities. The ratio of maximum wave potential energy to maximum landslide potential energy ranges between 0.011 and 0.059 and tends to be greater for shallower initial submergences. For two experimental configurations the ratio of maximum wave potential energy to maximum fluid kinetic energy is estimated to be 0.435 and 0.588. The wave trough initially generated above the rear end of the landslide propagates in both onshore and offshore directions. The onshore-propagating trough causes a large initial draw-down at the shore. The magnitude of the maximum draw-down is related to the maximum amplitude of the offshore-propagating first wave trough. A wave crest generated by the landslide as it decelerates at the bottom of the slope causes the maximum wave run-up observed at the shore. A semi-analytical model, based on inviscid and irrotational theory, is used to investigate the wave generation process of a moving submerged object in a constant depth channel. The simplified geometry allows a variety of phenomena, observed during the experimental tests, to be investigated further in a more controlled setting. The variations in the growth, magnitude, and decay of energy as a function of time is due the interaction of the pressure distribution surrounding the moving slider with the wave field, in particular, the leading crest and trough. The largest energy transfer between slider kinetic energy and wave potential energy occurs when there is prolonged interaction between the slider's low pressure region and the leading wave trough. The generation of onshore propagating waves by a decelerating landslide is confirmed, and the magnitude of the maximum wave run-up is found to be dependent on the magnitude of the slider deceleration. The model also shows that slides with Froude number close to unity convert substantial amounts of energy into offshore propagating waves. The onshore propagating wave potential energy is not as sensitive to Froude number. A further result from the model simulations is that the specific shape of the slider has only a minor influence on the wave response, provided the slider's length and area are known. A boundary element model, based on inviscid and irrotational theory, is used to simulate the laboratory experiments. Model predictions of the wave field are generally accurate, particularly the magnitude and range of wave amplitudes within the wave packet, the arrival time of the wave group, the amplitude of the run-up and run-down at the shore, the time the maximum run-down occurs, and the form and magnitude of the wave potential energy time history. The ratios of maximum wave potential energy to maximum slider kinetic energy are predicted to within ± 29%. The model predictions of the crest arrival times are within 3.6% of the measured times. The inability of the inviscid and irrotational model to simulate the flow separation and wake motions lead to a 45% under prediction of the maximum fluid kinetic energy. Both the semi-analytical and BEM models highlight the need for the correct specification of initial slider accelerations in numerical simulations in order to accurately predict the wave energy.

A new Laser Induced Fluorescence Capillary Zone Electrophoresis (LIF-CZE) bioassay to detect and study the catalytic activity of the sulfur assimilating enzyme commonly found in E. coli species; alkanesulfonate monooxygenase (EC 1.14.14.5) is described for the first time. This technique enables the possibility for direct injection onto a capillary for detection without the need for pre-concentration of sample and with minimal sample preparative steps prior to analysis. In this bioassay, a group of Fischer based cyanine dyes and two Oxazine (Nile red) derivatives were designed for further optimization as key Vis-NIR fluorescent substrate. In developing this technique, the test dyes were first assessed for their photophysical properties, based on four criteria; (1) photostable (2) solvatochromism (3) binding affinity towards both the monooxygenase active site and serum albumin and (4) chemical stability in strong electric field strength. Applying key dye characterization procedures including; molar absorptivity determination, quantum yield determination, photostability, solvatochromism and protein interaction studies it was determined that the Fischer indolium cyanine dyes were most suitable for the method development. The data revealed that under the test conditions, reduced flavin, the oxidative monooxygenase catalytically specifically converts the alkylsulfonate substituted cyanine dyes to the corresponding aldehyde. This new bioassay has proven to be quick, portable, sensitive, reliable and the exhibit the possibility of ‘on-the-spot’ detection; advantages not readily realized with other commonly applied techniques such as PCR, SPR, ELISA and GC used to study bacterial sulfur assimilation processes. In addition, recent literature results proposed by other research groups developing similar techniques showed strong reliance on GC analyses. Those assays involve the use of low molecular weight straight chain non-emissive alkanesulfonate substrates. Once enzyme catalysis occurs the aldehyde is formed becomes rather volatile and requires complex and tedious headspace sampling for GC analyses. This feature limits the in vitro applicability and eliminated the possibility in vivo development. Our goal is to further develop, optimize and present this CZE based bioassay as a suitable alternative to the current trends in the field while creating a more robust and sensitive in vitro monooxygenase detection method with the possibilities of in vivo application.

An isolated wing-tip vortex from a square-tipped NACA 0012 wing at an angle of attack of 5 degrees was studied in a water tunnel at a chord based Reynolds number of approximately 24000. Measurements were taken using stereo particle image velocimetry at three measurement planes downstream of the wing under each of three freestream turbulence conditions. The amplitude of wandering of the vortex axis increased with increasing distance downstream of the wing and with increasing freestream turbulence intensity. The magnitude of the peak azimuthal velocity decreased with increasing distance from the wing as well as with increases in the freestream turbulence intensity. The streamwise velocity in the vortex core was less than the freestream velocity in all cases. Time resolved histories of the instantaneous waveform shape and location of the vortex axis were determined from sequences of images of fluorescent dye released from the wing.

Natural turbulent convection studies encompass a wide range of flows occurring in nature, for example, atmospheric and oceanic flows, con-vection in the Earth’s mantle, convection in the stars and also in many engineering applications. Rayleigh-Benard convection (RBC), i.e. con-vection in a horizontal fluid layer confined between two plates with a temperature differential maintained across them, has been a proto-type problem in the studies of turbulent natural convection. Many small scale and global features of the flow in the turbulent regime of RBC are known, yet the flow dynamics is not fully understood, es-pecially at high Rayleigh numbers (Ra). Present work comprises of experimental investigations of a different type of flow, high Rayleigh number turbulent convection in a long vertical tube (abbreviated as tube convection or TC). The tube of aspect ratio (length to diameter) of about 10, open at both the ends interconnects two large tanks. The flow driven by an unstable density difference created between the two tanks, has some unique features, different from RBC. The net flow at any tube cross-section is zero and the time averages of the velocities, the Reynolds shear stress and the mean shear are also zero. Turbu-lent energy production is therefore solely due to buoyancy. The flow is axially homogeneous and axisymmetric. In the homogeneous region, the mean density gradient is linear. Rayleigh number in TC is conve-niently defined based on the mean (linear) density gradient (denoted by Rag). Two sets of experiments are carried out. In one set of experiments, the density difference is created using brine and fresh water and in another set, it is created using heat. The ranges of Rag achieved are 3 × 108 < Rag < 8 × 109 in the experiments using salt (Schmidt number, Sc ≈ 600) and 5 × 104 < Rag < 5 × 106 in the experiments using heat (Prandtl number, P r ≈ 6). From the measured salt and heat fluxes in both the sets of experiments, the non dimensional flux 1 1 scaling above a certain value of Rag is obtained as N ug ∼ Rag2 P r 2 and from the velocity measurements in the experiments using salt, the 1 Reynolds number scaling is obtained as Re ∼ Rag2 P r− 12 . Both these are as per the predicted scalings by the mixing length model proposed by Arakeri et al. (2000) for high Rag convection in the vertical tube. The flux scaling N u ∼ (RaP r)2 , also known as the ‘ultimate regime’ of convection, expected at very high Ra but not yet observed in the experiments in classical RBC, is easily achieved in TC at relatively lower values of Ra. The fluxes and Reynolds numbers in TC are orders of magnitude higher as compared to those obtained in RBC for similar values of Ra and P r. In the lower range of Rag values for P r ≈ 6, a transition to a new flux scaling, N u ∼ (RaP r)0.29 is found. Similar transitions are also found to be present in the results of Tovar (2002) for Sc ≈ 600 and in the DNS results of Schmidt et al. (2012) for P r = 1, at different values of Rag. Collecting all these data, it is shown that the transition occurs at a fixed Grashof number of 1.6 × 105, independent of P r. Velocity measurements are carried out using particle image velocime-try (PIV) in the salt experiments. Kinetic energy spectra computed from the velocity fields are presented for the locations from the tube axis to the wall, for the lowest and the highest values of Rag achieved in the experiments. The spatial energy spectrum of lateral velocity at the tube axis follows Kolmogorov-Obukhov (KO) scaling (−5/3 scaling exponent) while the spatial spectrum of longitudinal velocity shows a scaling slightly higher than −5/3 but lower than −11/5 (the Bolgiano-Obukhov (BO) scaling). The scalar spectra is computed from the concentration fields obtained from planar laser induced fluorescence (PLIF) in the experiments using salt, and also from the temperature measurements from the experiments using heat. Both the concentra-tion and temperature fluctuations spectra show some evidence of dual scaling - BO scaling (−7/5 scaling exponent) in the inertial subrange followed by Obukhov-Corrsin (OC) scaling (−5/3 scaling exponent) over a narrow range of scales. Light propagation through the buoyancy driven turbulent flow in TC has also been experimentally investigated. Light propagation through convective turbulence is encountered in many situations. In some cases e.g. in observational astronomy it is undesirable, while in some other cases it is useful, e.g. in remote sensing of meteorological parameters. In the present study, light intensity and angle of arrival fluctuations in a parallel beam of light are measured. Laser shadowgraphy is used in the intensity measurements while the angle of arrival is obtained by measuring deflections of narrow laser beams, created by passing collimated laser light through a mask having equispaced grid of holes. Background oriented schlieren (BOS) measurements have also been carried out to obtain the displacements, which are proportional to the angle of arrivals. The equations for frequency spectrum of intensity and angle of arrival from the literature, developed for isotropic, ho-mogeneous turbulent media, are modified for the flow in the present case and the asymptotic scalings for high and low frequency ranges are obtained. The scalings in the frequency spectra computed from the measurements of intensity and angle of arrival fluctuations are com-pared with the obtained asymptotic scalings. The results from the present work are also compared with results from studies in the atmo-sphere and lab experiments.

The present work is concerned with fundamental studies on the liquid fuel transport in the intake manifold of small carburetted engines. This work is motivated by the need for development of technologies to meet the stringent cold-start emission norms that are to be prescribed for two-wheelers in particular. More specifically, visualization studies conducted in a transparent manifold made of quartz in a small four-stroke 110-cc two-wheeler engine have shown the presence of gasoline films on the walls of the inlet manifold under cold start conditions. Advanced Laser diagnostic techniques such as Planar Laser Induced Fluorescence (PLIF) have been utilized to measure the thickness of the fuel films. The Sauter Mean Diameter for the fuel droplets at the carburettor exit is measured using Laser Shadowgraphy technique. It is observed that the films are present both at idling conditions and under load. This large amount of liquid fuel entering the engine leads to incomplete combustion and higher emissions of unburned hydrocarbons. A detailed analysis of the effects of heating the inlet manifold has been performed. The potential of this manifold heating strategy in reducing hydrocarbon emissions has been assessed and found to be promising. In addition, a need of proper control of the fuel exiting the carburettor is shown to reduce emissions and increase fuel efficiency.

An isolated wing-tip vortex from a square-tipped NACA 0012 wing at an angle of attack of 5 degrees was studied in a water tunnel at a chord based Reynolds number of approximately 24000. Measurements were taken using stereo particle image velocimetry at three measurement planes downstream of the wing under each of three freestream turbulence conditions. The amplitude of wandering of the vortex axis increased with increasing distance downstream of the wing and with increasing freestream turbulence intensity. The magnitude of the peak azimuthal velocity decreased with increasing distance from the wing as well as with increases in the freestream turbulence intensity. The streamwise velocity in the vortex core was less than the freestream velocity in all cases. Time resolved histories of the instantaneous waveform shape and location of the vortex axis were determined from sequences of images of fluorescent dye released from the wing.

La fluorescence induite par laser multibande est une technique non intrusive permettant d’accéder à la température de la phase liquide dans des milieux complexes. L’application de cette technique dans des liquides monophasiques où le chemin optique est variable (produit de la concentration moléculaire du traceur et de la distance dans le milieu liquide entre le volume de mesure et l’optique de détection) a été considérée. Le rapport des intensités de fluorescence collectées sur deux bandes spectrales permet de s’affranchir de la concentration en traceur fluorescent, de l’intensité laser incidente et du volume de mesure. Une troisième bande spectrale de détection permet de prendre en compte le terme de ré-absorption de la fluorescence dans le cas de chemins optiques non-négligeables et variables. Puis l’application de la technique à la mesure de la température de la phase liquide d’un spray est présentée. Un traitement spécifique du signal a été développé afin de tenir compte de la nature aléatoire du signal de fluorescence liée à la présence de gouttes dans le volume de mesure. De plus, ce traitement a été adapté afin de rendre possible le couplage des données de fluorescence avec des mesures granulométriques effectuées par la technique phase Doppler dans la perspective finale d’obtenir des températures de goutte par classe de taille. Cependant plusieurs phénomènes perturbateurs ont été mis en évidence : - une dépendance inattendue et non linéaire à la taille de goutte du rapport des intensités de fluorescence collectées sur deux bandes spectrales. - une forte diffusion de la lumière laser incidente par le nuage de gouttelettes induit une fluorescence bien au delà de la zone d’excitation laser. Cette fluorescence parasite est néanmoins détectée dans la profondeur de champ du dispositif optique et se conjugue avec l’effet non-linéaire de la taille des gouttes. Une stratégie de correction de ces différents phénomènes est proposée et une expérience de validation est réalisée sur un spray chauffé injecté dans une cellule saturée en vapeur / Multiband laser-induced fluorescence is a non-intrusive technique able to provide a measurement of the liquid phase of complex media. The application of this technique in single phase liquids, with a variable optical path (product of the fluorescent tracer molecular concentration by the distance between the probe volume and the collection optics in the liquid) was considered. The ratio of the fluorescence intensities collected on two spectral bands allows removing the influence of the fluorescence tracer concentration, incident laser intensity and probe volume. A third spectral band of detection is used to take into account the re-absorption of the fluorescence in the case of non negligible and variable optical paths. Then, the application of this technique to the measurement of the temperature of the liquid phase of a spray is presented. A specific data processing was developed in order to take into account the random presence of droplets in the probe volume. Moreover, the processing was adapted to achieve combined fluorescence and droplet size measurements using the phase Doppler technique. The overall foreseen goal is to measure temperature per droplet size class. However, several disturbing phenomena were highlighted: - an unexpected non-linear dependence on the droplet size of the ratio of the fluorescence intensities collected on two spectral bands, - a strong incident laser light scattering by the droplets cloud, which induces a fluorescence beyond the excitation zone. This fluorescence is also collected in the depth of field of the optical device and combines with the non-linear size dependence. A correction strategy of these phenomena was implemented and a validation experiment on a heated spray injected in a vapour-saturated cell was performed

L’étude des transferts de chaleur et de masse lors de l’évaporation de gouttes en mouvement et en interaction est un domaine complexe à cause des nombreux phénomènes en jeu. Les principaux paramètres influençant l’évaporation ont pu être étudiés indépendamment grâce à l’utilisation de diagnostics optiques de mesure non-intrusifs sur un train de gouttes monodisperse. Une technique basée sur la fluorescence induite par laser (LIF) à deux couleurs a été développée afin d’obtenir la température moyenne de gouttes de combustible mono et multicomposant. Afin de supprimer l'effet optique parasite engendré par des résonances morphologiquement dépendantes, un absorbeur non fluorescent a été ensemencé à faible concentration. L’évolution de la vitesse et de la taille des gouttes ont été investiguées grâce à une technique par ombroscopie quantitative qui permis la mesure très précise des taux d’évaporation. Une enceinte à haute température a été conçue afin de générer des conditions ambiantes maitrisées et propices à la formation d’une forte évaporation. Ainsi, les nombres de Nusselt et Sherwood ont été déterminés expérimentalement pour plusieurs combustibles dans diverses conditions d'injection. L'étude sur différents combustibles et à différentes températures d'injection a confirmé l’influence de la volatilité du combustible sur les transferts. L’influence du nombre de Reynolds a aussi été mise en évidence. L’étude de gouttes multicomposant a permis de montrer différentes phases d’échauffement et d’évaporation lors du temps de transit de la goutte liées aux différences de volatilité des combustibles du mélange. Les effets de différentes compositions ont aussi été investigués / The study of heat and mass transfers during the evaporation of moving and interacting droplets remain a complex field because of the various mechanisms in action. The main parameters influencing the evaporation of droplets have been studied separately thanks to non intrusive optical diagnostics that have been used on a monodisperse droplet stream. A technique based on two colors laser induced fluorescence (LIF) was developed to measure the temperature of mono and multicomponent evaporating fuel droplets. The liquid fuel is seeded by a non-fluorescent absorber to eliminate the effect of morphological dependant resonances. The size evolution was obtained thanks to shadow imaging which allowed precise measurements of evaporation rates. A hot chamber was conceived to create controlled ambient conditions around the droplets. Thereby, the Nusselt and Sherwood numbers, characterizing the heat and mass transfers, were deduced from the experimental data for various experimental conditions. The studies allowed confirming the influence of the volatility of the fuel regarding heat and mass transfers. The results also exhibit an influence of the Reynolds number. Finally, the study of multicomponent droplets had shown different heating and evaporating phases during the droplet transit time. Effects of various compositions have also been investigated