Thesis (Doctoral)--Universität Zürich, 1976.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted. Incorrect ISBN on t.p. verso.

Thesis (Doctoral)--Universität Zürich, 1976.

Thesis (doctoral)--University of Helsinki, 1989. / Includes bibliographical references (p. 92-101).

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted. Incorrect ISBN on t.p. verso.

Thesis (Ph.D.) -- McMaster University, 1999. / Includes bibliographical references (leaves 212-219). Also available via World Wide Web.

Made available in DSpace on 2015-12-10T14:22:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-23. Added 1 bitstream(s) on 2015-12-10T14:29:04Z : No. of bitstreams: 1 000851515.pdf: 761120 bytes, checksum: 102e5964647d8ea3227709946562ac69 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Neste trabalho foi investigado a participação de MMP's na intoxicação aguda por chumbo, bem como se a doxiciclina reverte a hipertensão induzida pelo chumbo e a alteração dos níveis plasmáticos de CGRP- (peptídeo relacionado ao gene da calcitonina), adrenomedulina ou endotelina após hipertensão induzida pelo chumbo. Utilizamos 40 ratos machos Wistar adultos jovens (pesando 200g). Grupo Pb (n=12) foi intoxicado com acetato de chumbo (1ª dose 8 μg /100g do rato, e subsequentes doses 0.1μg/100g intra-peritoneal para cobrir a perda diária, durante sete dias) e tratados com doxiciclina 30mg/kg/dia do rato (Pb+Doxy = 12) via oral uma vez ao dia. Grupo controle (n=8) recebeu acetato de sódio para oferecer quantidade equimolar de moléculas de acetato do grupo Pb. Grupo controle também foi tratado com doxiciclina (Grupo Doxy, n=8). Todos os tratamentos foram realizados por sete dias. A concentração em chumbo em sangue total foi determinada em forno de grafite por espectrometria de absorção atômica. Concentrações plasmáticas de adrenomedulina, CGRP, endotelina e MMP-2 foram avaliadas usando kits comerciais de imunoensaio enzimático (ELISA). O comitê de ética institucional (protocolo nº 458/2013) aprovou todos os experimentos. Houve aumento na pressão sistólica (grupo Pb 167 ± mmHg vs. 142 ± 2 ou 143 ± 3 mmHg no grupo controle) (P<0.05), entretanto, doxiciclina mostrou diminuir valores (137 ± 3 mmHg no grupo Pb+Doxy). A concentração de chumbo no sangue foi significativamente aumentada em ambos os grupos intoxicados (Pb e Pb + Doxy) que apresentou 10μg/dL (± 1,0) vs. 2,0 μg/dL (± 1,0) em grupos não intoxicados (grupos Controle ou Doxy). Doxiciclina reverteu a redução de 45% nos níveis de adrenomedulina induzido pelo chumbo (grupo Pb apresentou 0.43 (± 0,2) vs. 1.0 pg/mL (± 0,2) em grupo Pb+Doxy). Não observamos mudança nos níveis plasmáticos de CGRP, endotelina e MMP-2. Sugerimos que a hipertensão... / In this work was investigated the participation of MMP's in acute lead intoxication, as well as if doxycycline reverses the lead-induced hypertension,and whether there arechanges in CGRP (calcitonin gene related peptide),adrenomedullin or endothelinplasma levels afterlead-induced hypertension.40 Wistar young adult male rats(weighting 200g) were used. Pb group (n=12) was intoxicated with lead acetate (1st dose 8μg/100g of body weight, and subsequent doses 0.10 μg/100g, intra-peritoneal to cover daily loss, during seven days) and treated with doxycycline (30mg/kg of body weight) (Pb+Doxy group, = 12) or saline (Pb group, = 12) by gavage, once a day. Control groups (n=8) received sodium acetate in order to offer the same amount of acetate molecules asPb group. Control group was also treated with doxycycline (Doxy group, n=8). All treatments were performed for seven days. Lead concentrations in whole blood were determined by graphite furnace atomic absorption spectrometry. Plasma adrenomedullin, CGRP, endothelin and MMP-2 concentrations were evaluated using commercials enzyme immunoassays (ELISA) kits. All experiments were approved by the Institution ethics committee (protocol nº 458/2013). There was an increase (P<0.05) in the systolic blood pressure of Pb group (167 mmHg vs. 142 ± 2 ou 143± 3 mmHg in Control group), however, doxycycline showed a decrease in these values (137± mmHg in Pb+Doxy group). The lead concentrations in blood were significantly increased in both intoxicated groups (Pb and Pb+Doxy groups) which presented 10μg/dL±1,0 μg/dLvs. 2μg/dL±1,0 μg/dL, in non-intoxicated groups (Saline or Doxy groups). Doxycycline reversed the reduction of 45% in the levels ofadrenomedullin induced by lead (Pb group presented 0.43 pg/mL vs. 1.0 pg/mL in Pb+Doxy group). We did not observe changes in the plasmatic levels of CGRP, endothelin and MMP-2.We suggest that hypertension induced by lead exposure (8 μg/100g ...

Trace elements, because they are effective in catalytic amounts, must be held in delicate balance with each other as well as with the macronutrients of the diet. Disturbances of these balances result in deficiency and toxicity symptoms. Toxic doses of selenium, lead, and zinc, for example, are known to cause anemia (1, 2, 3, 4) and growth depression (5, 3) in experimental animals.

Hair from small mammals of roadside, urban parkland, wilderness, and antique populations was analyzed for lead by atomic absorption spectrophotometry. Hair from roadside populations had a significantly greater amount of lead than hair from the ether groups. Hair from antique populations did not differ in lead content from that of current populations which are removed from immediate exposure to automotive exhausts.

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