published_or_final_version / Orthopaedics and Traumatology / Master / Master of Philosophy

Fast medio-lateral movements, frequent in a number of sports activities, are associated with lower extremity injuries. These injuries may occur as a result of excessive musculoskeletal stresses on the joints and their associate structures. The purpose of this study was to investigate the effect of running speed and turning movement on the three-dimensional moments at the ankle, knee, and hip joints. Data were collected using video cameras and force plate. Eight male recreational basketball players were tested during slow (1.5 m/s), moderate (3.0 m/s), and fast running (4.5 m/s) and when cutting to the right or left (+60, +30, 0, -30, and -60��). The inverse dynamics approach was used to integrate the body segment parameter, kinematic and force plate data, and to solve the resultant joint moments. At the ankle joint, inversion/eversion, dorsi/plantar flexion, and internal/external rotation moments of the ankle joint increased with running speed (p<.05). At the knee joint, flexion/extension and abduction/adduction moments increased with running speed except flexion moment that decreased with running speed (p<.05). At the hip joint, internal/external rotation, flexion/extension, and abduction/adduction moments increased with running speed (p<.05). In medial cutting movements, greater abduction moments of the ankle, adduction moments of the knee and external rotation and adduction of the hip were found (p<.05). In lateral cutting movements, greater inversion and adduction moments of the ankle, abduction moments of the knee and hip were found (p<.05). These findings reinforce the intuitive notion that fast medio-lateral turning movements produce substantially greater musculoskeletal loading on the joint structures than does straight running and consequently have greater potential for inducing lower extremity injuries such as ankle sprain or anterior cruciate ligament injury. / Graduation date: 1999

This thesis presents a method for determining range of body motion for a walking machine with feet fixed on the ground. The darkling beetle was selected as the sample subject in this study. A closed form inverse kinematic solution is used to determine if a point in space is within range of body motion. An algorithm for tracing workspace boundary is also presented. The software, developed in Microsoft QuickC, has three main parts: (1) a module for searching the workspace contours and recording the contour points, (2) a plotting program for presentation of the workspace on the screen, (3) a module to determine ranges of roll, pitch and yaw for specified foot positions. The plotting program shows four views of the workspace, including front, top, and side views, and user specified axonometric projection. Body range of motion for a representative set of foot positions is presented and analyzed. Results are presented for normal resting height (10mm) and for 8mm and 12mm heights. Body range of motion for feet positioned for the alternating tripod gait is also presented. Ranges of roll, pitch and yaw have been determined and are discussed. / Graduation date: 1991

This thesis examines the influence on range-of-motion of beetle body of changes in leg segment parameters. From beetle's leg orientation, influence of the following leg segment parameters are investigated: coxa length, coxa twist and body-coxa joint. Kinematic equations are derived for legs of the beetle. Roots of quartic polynomials obtained while solving the kinematic equations are found by using the Bairstow (1966) numerical method. Inverse kinematic solutions are obtained for each leg and used to determine whether a point is within the body range of motion or not. An algorithm developed by Mason (1957) and Cordray (1957) for tracing closed boundaries is used to find ranges of motion of the body and feet. Changes in body range of motion caused by alteration in leg segment parameters are complex and not easy to explain. Similarities between changes in body range of motion and foot range of motion are observed. A great deal more work is necessary to fully understand the importance of observed changes. / Graduation date: 1992

For animals to walk properly, motor neurons (MNs) need to reach their target muscles in the leg and they need to arborize properly. Cell adhesion molecules (CAMs) have pivotal roles in a variety of neural development processes including arborization. Two Immunoglobulin Super Family (IgSF) CAMs, DIP-α and its interacting partner Dpr10, have been shown to play key roles in leg MN arborization in flies. DIP-α is expressed by three leg MNs and dpr10 is expressed by leg muscles. When either DIP-α or dpr10 are removed in flies MNs reach their target but fail to arborize their branches. Interestingly, DIP-α and Dpr10 have some of the highest binding affinities relative to other DIPs and Dpr interacting pairs. Therefore, in this thesis I ask how important are these relatively high affinities and do the MNs have different affinity requirements? I show that decreasing and increasing the affinity between these two proteins in vivo has adverse effects on MN arborization and that different MNs rely on different thresholds of DIP-α::dpr10 binding affinities to arborize properly. Based on these experiments, I then ask how DIPs and Dprs interact when they are co-expressed by the same neuron. Here I provide evidence that altering the ratio of DIP-a::dpr10::dpr6 can inhibit DIP-α’s ability to bind in trans with Dpr10. Furthermore, together with Dr. Nick Morano in the Shapiro lab, we show that DIP-α has a longer linker domain compared to DIPs that are not able to bind in cis and that removing this linker domain in vivo phenocopies some of the effects seen by removing co-expressed dpr6 or dpr10 in DIP-α MNs. Therefore, although DIP-α and its partners Dpr6 and Dpr10 are expressed in MNs, relative concentrations and cis interactions in MNs dictate how DIP-α interacts in trans with Dpr10. Together, this thesis provides a better understanding as to how interactions between CAMs can be modified via affinities, concentration, and co-expression in vivo to ultimately shape axon morphology of Drosophila leg MNs.

The purpose of this study was to examine the relationship between leg dominance and knee mechanics to provide further information about the etiology of ACL injury. Sixteen healthy females between the ages of 18 and 22 who were NCAA Division I varsity soccer players participated in this study. Subjects were instructed to perform a cutting maneuver; where they sprinted full speed and then performed an evasive maneuver (planting on one leg and pushing off to the other leg in a new direction) at a 45° angle with their dominate leg (DL) and non-dominate leg (NDL). Subjects were required to perform five successful cuts on each side given in a random order. Bilateral kinematic and kinetic data were collected during the cutting trials. After the cutting trials, subjects performed bilateral isometric and isokinetic testing using a Cybex Norm dynamometer at a speed of 60°/sec to evaluate knee muscle strength. During the braking phase the NDL showed greater (P=0.003) power absorption, greater (P=0.01) peak internal rotation angle and greater (P=0.005) peak flexion velocity. During the propulsive phase the DL showed greater (P=0.01) power production, greater (P=0.038) peak internal adductor moment and greater (P=0.02) peak extension velocity. In addition, no differences (P>0.05) in knee extensor and flexor isometric and isokinetic torques between the two limbs were shown. The results of this study show that a difference in knee mechanics during cutting does exist between the DL and NDL. The findings of this study will increase the knowledge base of ACL injury in females and aid in the design of more appropriate neuromuscular, plyometric and strength training protocols for injury prevention. / School of Physical Education, Sport, and Exercise Science

Two experiments were undertaken. The objective of Experiment One was to identify the lower limb muscles that were most frequently active during the early period of a step turning task for further testing in Experiment Two. In Experiment Two participants undertook multiple trials of a step-turning task, 30 and 60° to the left and right of midline, at a self-selected pace in response to a visual cue. There were five objectives to Experiment Two. Firstly, to identify the predominant order in the onset of foot movement so that anticipatory muscle activity could be defined for this task. Secondly, to identify whether there is a consistent temporal order in movement onset between the head and the feet. Thirdly, to identify whether and how consistently anticipatory lower limb muscle activity is present bilaterally. Fourthly, to assess whether there is a consistent sequence in the onset of anticipatory muscle activity among muscles active in at least 80% of trials. The final objective was to identity whether there was a consistent temporal relationship in the onset of the anticipatory muscle activity present in at least 80% of trials, with the onset of head and foot movement. Study Design: A repeated measures design was used. Background: Anticipatory lower limb muscle activity in gait initiation and forward stepping studies has been reported to be consistently present, and associated with initial and important balance responses. Falls during turning are associated with a high incidence of hip fractures in the elderly population. The presence of anticipatory lower limb muscle activity turning has not been previously reported. Participants: There were five participants in Experiment One, and ten in Experiment Two. All were between 18 and 40 years of age and did not have neurological or musculoskeletal disorders, or severe visual loss. Results: In Experiment One, four muscles were consistently active bilaterally, during the early period of step-turning and were: tibialis anterior, gastrocnemius, biceps femoris and gluteus medius. In Experiment Two the ipsilateral foot moved before the contralateral foot in 68% of trials towards the left, and 79% of trials towards the right. The onset of head movement consistently occurred before the onset of foot movement during turns towards both directions. The percentage of trials in which the four muscles were active in an anticipatory manner was low bilaterally, ranging from 12 to 38% of trials. Objectives that involved the further analysis of muscles active in at least 80% of trials were unable to be completed. Conclusions: During a step-turning task young healthy adults predominantly move their ipsilateral foot before their contralateral foot. The consistent onset of head movement prior to that of the feet, indirectly suggests that the visual system might influence the temporal onset of the feet. The low levels of anticipatory muscle activity during step-turning suggest that the lower limbs are not involved with the initial balance responses for this task thus making it inherently different to gait initiation and forward stepping.

This thesis investigates a simple dynamic model of the hind leg of a beetle during initiation of a step. The primary assumption was that the full load of the body was carried on the hind leg during this time. That is, the only forces on the body were that of the hind leg and gravity and their resultant produced forward acceleration. Only two dimensional models were used in this study. This was justified since the beetle is bilaterally symmetrical. However, it required the assumption that hind legs were positioned symmetrically and it limited the investigation to forward acceleration in a straight line. Models with two and three links were tested. The two link model assumed the body has no motion relative to the upper legs; that is the muscles were strong enough to prevent movement at the joint between body and leg. The three link model assumed only friction prevented movement at the joint between body and leg. Dynamic equations were developed using Lagrangian mechanics. These equations were integrated using the 4th order Runge-Kutta algorithm. Both models were driven by applying a constant torque at the joint between upper and lower segments. Driving torque was adjusted to minimize verical movement of body center of mass. Initial position of body center of mass relative to foot was varied to examine it's influence on both horizontal travel of body, center of mass and driving torque required for this travel. For both models horizontal travel was less dependent on initial height of body center-of-mass than on initial horizontal position. For both models required driving torque increased with decrease in initial height of body center-of-mass and with increase of initial horizontal distance from foot to body center-of-mass. For both models maximum horizontal travel was attained with minimum initial height of body center-of-mass and minimum initial horizontal distance between foot and body center-of-mass. For the two link model, maximum horizontal travel was approximately half of the total leg length while for the three link model the equivalent number was approximately one quarter, of total leg length. / Graduation date: 1992

The purpose of this study was to determine the jump landing impact absorption kinematic and kinetic characteristics of the four subject groups: a) male high school athletes, b) female high school seasonal athletes, c) female high school year-round athletes, and d) female college athletes who train year-round. Subjects dropped onto a force platform from a height of 40 centimeters. Variables analyzed were flexibility, strength, Fz, Fy, and Fx force, free moment, range of motion, contact and maximum angles, time to maximum angle, contact and maximum angular velocities, and time to maximum angular velocity. Significant differences among subject groups were determined through use of a factorial analysis of variance and Scheffe' post hoc test.A difference was noted in the landing patterns of males and females. Male subjects exhibited a significantly longer time to first vertical peak force. This longer time may allow for greater initial force attenuation; however, the male athletes had a significantfy greater second peak force. Males also differed significantly from females in anteroposterior and mediolateral force.The differences in method of force attenuation may be due to significantly greater ankle eversion shown by the males and greater knee flexion used by the females. Additionally, the males had significantly greater leg strength, which may have allowed them to increase the time to first peak.The year round female high school athletes were found to use the best biomechanical landing method. They had a greater range of motion in the knee and ankle, and subsequently produced the lowest amount of vertical and anterio-posterior force. / School of Physical Education

Two experiments were undertaken. The objective of Experiment One was to identify the lower limb muscles that were most frequently active during the early period of a step turning task for further testing in Experiment Two. In Experiment Two participants undertook multiple trials of a step-turning task, 30 and 60° to the left and right of midline, at a self-selected pace in response to a visual cue. There were five objectives to Experiment Two. Firstly, to identify the predominant order in the onset of foot movement so that anticipatory muscle activity could be defined for this task. Secondly, to identify whether there is a consistent temporal order in movement onset between the head and the feet. Thirdly, to identify whether and how consistently anticipatory lower limb muscle activity is present bilaterally. Fourthly, to assess whether there is a consistent sequence in the onset of anticipatory muscle activity among muscles active in at least 80% of trials. The final objective was to identity whether there was a consistent temporal relationship in the onset of the anticipatory muscle activity present in at least 80% of trials, with the onset of head and foot movement. Study Design: A repeated measures design was used. Background: Anticipatory lower limb muscle activity in gait initiation and forward stepping studies has been reported to be consistently present, and associated with initial and important balance responses. Falls during turning are associated with a high incidence of hip fractures in the elderly population. The presence of anticipatory lower limb muscle activity turning has not been previously reported. Participants: There were five participants in Experiment One, and ten in Experiment Two. All were between 18 and 40 years of age and did not have neurological or musculoskeletal disorders, or severe visual loss. Results: In Experiment One, four muscles were consistently active bilaterally, during the early period of step-turning and were: tibialis anterior, gastrocnemius, biceps femoris and gluteus medius. In Experiment Two the ipsilateral foot moved before the contralateral foot in 68% of trials towards the left, and 79% of trials towards the right. The onset of head movement consistently occurred before the onset of foot movement during turns towards both directions. The percentage of trials in which the four muscles were active in an anticipatory manner was low bilaterally, ranging from 12 to 38% of trials. Objectives that involved the further analysis of muscles active in at least 80% of trials were unable to be completed. Conclusions: During a step-turning task young healthy adults predominantly move their ipsilateral foot before their contralateral foot. The consistent onset of head movement prior to that of the feet, indirectly suggests that the visual system might influence the temporal onset of the feet. The low levels of anticipatory muscle activity during step-turning suggest that the lower limbs are not involved with the initial balance responses for this task thus making it inherently different to gait initiation and forward stepping.