The inconstancy of legume seed set has been recognized for some time, but the basic factors responsible for this irregularity have remained somewhat of a mystery. The literature on the subject is quite extensive and contains a diversity of opinions. Oddly enough the role of micronutrients seems scarcely to have been considered as a possible basic factor in legume seed setting. A study therefore has been made of the effects certain elements have on legume seed yields. The addition of lime to plots provided with molybdenum or phosphate on an acid clay soil in the Fraser Valley of British Columbia, with 900-1200 P.P.M. available calcium, markedly increased red clover seed yields. Without lime, the only response was obtained from additions of phosphate and potash. The addition of nitrogen to plots, on similar soil, which received molybdenum, sulphur, and phosphate resulted in markedly increased seed yields of double-cut red clover over the control. Without nitrogen there was a greater response on this soil to additions of phosphate, molybdenum and sulphur. The response to molybdenum was much greater, in both seed and forage, where plants were not provided with combined nitrogen, but where they largely depended on symbiotic nitrogen fixation for their nitrogen supply. The increased yield from sulphur attributable to this element having an indirect effect upon legumes through its direct' action, or effect, upon the nitrogen fixing organisms. Pole beans grown on a sandy loam soil with a pH 5.5 and 900 P.P.M. "available" calcium, produced more seed following applications of phosphate. The response was noted at several levels of lime and nitrogen. Molybdenum with lime, and sulphur with nitrogen also Improved seed yields. On a clay soil with a pH 4.8 and 500 P.P.M. "available calcium" the addition of lime increased all seed yields regardless of other treatments. The only other treatment to show a uniform and greatly increased seed yield, was an application of boron. On this clay soil pole bean plots receiving nitrogen outyielded the "no nitrogen" plots, but only phosphate, or potash, in combination with nitrogen showed much higher yields than the check. An experiment with double-cut red clover on a sandy loam soil with pH 5.5 and 900 P.P.M. "available" calcium showed a direct response to nitrogen regardless of the micronutrients added. On this soil boron with molybdenum gave higher seed yields than boron or molybdenum alone. These results were eon-firmed by a greenhouse experiment using silica sand. / Land and Food Systems, Faculty of / Graduate

Examination of Vicia amen'cana and Astragalus miser var. serotinus for estrogenic and anti-estrogenic activity demonstrated low potency uterotrophic compounds interfering with synthetic hormones in mammalian reproductive tracts. The potency of the extracts was affected by stage of growth. A toxic fraction was present in Astragalus miser var. serotinus at full bloom stage. Hormonal activity was not correlated with proximate analysis results for both species. Examination of the extract components revealed the overall structures of phenolic and aromatic compounds, including isoflavones, in a dynamic state throughout the growing season. The effects of topical fertilizer application on alsike clover (Trifolium hybridum), ladino clover (Trifolium repens var. ladino) and alfalfa (Medicago sativa) indicated that dry matter yields were slightly but insignificantly affected by fertilizer application. A method for the quantitative analysis of the free estrogenic isoflavones biochanin A and genistein was developed. Estimation of these two isoflavones, in addition to coumestrol and formononetin, illustrated that N, P and K applications would significantly affect the level of these plant sterols in the legume species. Alsike clover increased in total isoflavone content with phosphate addition; ladino clover increased total isoflavones to phosphate deficiency and complete fertilizers; alfalfa did not respond to fertilizer treatment. Total estrogenic compounds on a dry matter basis were less affected in the three species than were individual isoflavone components. / Land and Food Systems, Faculty of / Graduate

No description available.

Thesis (Ms. Sc)--University of Western Sydney, Hawkesbury, 1994. / "A thesis submitted in fulfillment of the requirements for the degree of Master of Science (Hons.) in Environmental Sciences." Includes bibliographical references (p. 170-177).

Lau Wing-Sze. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 131-149). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abstract (Chinese version) --- p.iv / Table of Contents --- p.vi / List of Tables --- p.x / List of Figures --- p.xii / List of Abbreviations --- p.xiv / Chapter Chapter One: --- An overview of Vigna sinensis seeds / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Food and functional food --- p.2 / Chapter 1.3 --- Edible legumes as an important food --- p.4 / Chapter 1.4 --- Nutritional an extra-nutritional values of V. sinensis seeds --- p.5 / Chapter Chapter Two: --- Purification of phenolic antioxidants from V. sinensis seeds / Chapter 2.1 --- Introduction --- p.11 / Chapter 2.1.1 --- Reactive oxygen species and antioxidants --- p.12 / Chapter 2.1.2 --- Phenolic flavonoids --- p.15 / Chapter 2.2 --- Materials and Methods / Chapter 2.2.1 --- Chemicals and reagents --- p.24 / Chapter 2.2.2 --- Plant material --- p.25 / Chapter 2.2.3 --- Optimization and extraction of V. sinensis seeds constituents --- p.25 / Chapter 2.2.4 --- Chromatographic separation of phenolic components --- p.26 / Chapter 2.2.5 --- Determination of phenolic contents --- p.27 / Chapter 2.2.6 --- Determination of free radical scavenging ability using trolox equivalent antioxidant capacity (TEAC) assay --- p.28 / Chapter 2.2.7 --- Statistical analysis --- p.29 / Chapter 2.3 --- Results and Discussion / Chapter 2.3.1 --- Optimization on the extraction of V. sinensis seeds --- p.30 / Chapter 2.3.2 --- Extraction and fractionation of V. sinensis seeds constituents --- p.31 / Chapter 2.3.3 --- Yield of various V sinensis seed fractions --- p.31 / Chapter 2.3.4 --- Phenolic contents in various V. sinensis seed fractions --- p.32 / Chapter 2.3.5 --- Free radical scavenging abilities of various V sinensis seed fractions --- p.33 / Chapter Chapter Three: --- Effect of V. sinensis seed extract on high fat and cholesterol - feeding mice / Chapter 3.1 --- Introduction --- p.41 / Chapter 3.1.1 --- Cholesterol in bloodstream circulation --- p.42 / Chapter 3.1.2 --- "Relationship between LDL oxidation, atherosclerosis and coronary heart disease" --- p.43 / Chapter 3.1.3 --- Diet supplements with beneficial effects on preventing coronary heart disease --- p.44 / Chapter 3.2 --- Materials and Methods --- p.47 / Chapter 3.2.1 --- Chemicals and reagents --- p.47 / Chapter 3.2.2 --- Preparation of diets --- p.48 / Chapter 3.2.3 --- Animals --- p.48 / Chapter 3.2.4 --- Feeding experiments --- p.49 / Chapter 3.2.5 --- Post-feeding analysis --- p.50 / Chapter 3.2.5.1 --- Caecal content and health indices --- p.50 / Chapter 3.2.5.2 --- Serum triglycerides --- p.51 / Chapter 3.2.5.3 --- Serum total cholesterol --- p.52 / Chapter 3.2.5.4 --- High-density lipoprotein (HDL) cholesterol --- p.53 / Chapter 3.2.5.5 --- Low-density lipoprotein (LDL) cholesterol --- p.54 / Chapter 3.2.5.6 --- Hepatic lipid and cholesterol --- p.55 / Chapter 3.2.6 --- Statistical analysis --- p.55 / Chapter 3.3 --- Results and Discussion --- p.56 / Chapter 3.3.1 --- Food intakes and body weights of animals --- p.56 / Chapter 3.3.2 --- Caecal contents and health indices --- p.56 / Chapter 3.3.3 --- Effects of V sinensis seed extract on serum and hepatic levels of triglycerides and cholesterol --- p.57 / Chapter Chapter Four: --- Antiproliferative activities of V. sinensis seed extracts / Chapter 4.1 --- Introduction --- p.66 / Chapter 4.1.1 --- Cancer and antioxidant --- p.67 / Chapter 4.1.2 --- Dietary cancer prevention agents --- p.68 / Chapter 4.2 --- Materials and Methods --- p.71 / Chapter 4.2.1 --- Chemicals and reagents --- p.71 / Chapter 4.2.2 --- Cell lines --- p.71 / Chapter 4.2.3 --- Maintenance of cell lines --- p.72 / Chapter 4.2.4 --- Antiproliferation assays --- p.73 / Chapter 4.2.4.1 --- MTT assay --- p.73 / Chapter 4.2.4.2 --- BrdU assay --- p.73 / Chapter 4.2.5 --- Cytotoxic activity determined by lactate dehydrogenase (LDH) assay --- p.77 / Chapter 4.2.6 --- Time-course assay --- p.79 / Chapter 4.2.7 --- Determination of IC50 --- p.79 / Chapter 4.2.8 --- Statistical analysis --- p.79 / Chapter 4.3 --- Results and Discussion --- p.80 / Chapter 4.3.1 --- Antiproliferative activities of V. sinensis seed extracts on HepG2 cells --- p.80 / Chapter 4.3.2 --- Cytotoxic activities of V. sinensis seed extracts on HepG2 cells --- p.82 / Chapter 4.3.3 --- Antiproliferative activities of phenolic fraction on MCF-7cells --- p.83 / Chapter 4.3.4 --- Cytotoxic activity of phenolic fraction on MCF-7 cells --- p.84 / Chapter 4.3.5 --- Time-course study of antiproliferative activities of phenolic fraction on cancer cells --- p.85 / Chapter 4.3.6 --- Effect of phenolic fraction on normal cells --- p.86 / Chapter Chapter Five: --- Antioxidant and antiproliferative activities of selected content flavonoids from V. sinensis seeds / Chapter 5.1 --- Introduction --- p.93 / Chapter 5.1.1 --- Cell cycle progression and regulation --- p.94 / Chapter 5.1.2 --- Bioavailability of plant flavonoids --- p.96 / Chapter 5.1.3 --- Characterization of flavonoids in V. sinensis seed --- p.98 / Chapter 5.2 --- Materials and Methods --- p.102 / Chapter 5.2.1 --- Chemicals and reagents --- p.102 / Chapter 5.2.2 --- Determination of free radical scavenging ability of identified flavonoids from V sinensis seeds using trolox equivalent antioxidant capacity (TEAC) assay --- p.103 / Chapter 5.2.3 --- Antiproliferation assays --- p.104 / Chapter 5.2.4 --- Cytotoxicity assay --- p.104 / Chapter 5.2.5 --- Time-course assay --- p.104 / Chapter 5.2.6 --- Determination of cell cycle distribution by flow cytometry --- p.105 / Chapter 5.2.7 --- Statistical analysis --- p.106 / Chapter 5.3 --- Results and Discussion --- p.107 / Chapter 5.3.1 --- Free radical scavenging abilities of identified flavonoids from V sinensis seeds --- p.107 / Chapter 5.3.2 --- Antiproliferative activities of selected flavonoids on cancer cells --- p.109 / Chapter 5.3.3 --- Cytotoxic activities of selected flavonoids on cancer cells --- p.111 / Chapter 5.3.4 --- Time -course study of antiproliferative activities on cancer cells --- p.112 / Chapter 5.3.5 --- Cytotoxic activities of selected flavonoids on normal cells --- p.114 / Chapter 5.3.6 --- Determination of the effects of cyanidin on cancer cells by analyzing cell cycle pattern --- p.115 / Chapter Chapter Six: --- Conclusion --- p.128 / References --- p.131

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 182-187).

Call number: LD2668 .T4 1932 T61

Research was conducted in the summers of 1985 and 1986 at the University of Arizona Marana Agricultural Center, with the objectives of (1) determining the best intercropping species combination under near optimum irrigation, using three cereals (sorghum (Sorghum bicolor), maize (Zea mays), and pearl millet (Pennisetum americanum) and three legumes (field bean (Phaseolus vulgaris), cowpea (Vigna unguiculata), and soybean (Glycine max) in all combinations, and (2) identifying species genotypes best adapted to intercropping. Results from 1985 determined sorghum x soybean as the most appropriate intercropping combination for the environment of the Marana Agricultural Center. In the 1986 cropping season, three sorghum genotypes (Pioneer 8493, Funks G-522DR, and California IO80H40) were combined with three soybean genotypes (Asgrow A6242, Asgrow A6520, and Rillito), to identify the best genotype combination for intercropping. Both 1985 and 1986 experiments were carried out in a randomized complete block design with four replications. Pearl millet was the cereal with the greatest decrease in yield when intercropped, indicating that this cereal was not a good competitor with legumes. Sorghum was the best cereal competitor with the legumes and soybean was the best legume competitor with the cereals. Among the three sorghum genotypes studied in 1986, only Pioneer 8493 showed higher yield in monocrop whereas the other two genotypes yielded higher in intercropping, indicating some benefit from this system. On the average, all three sorghum genotypes showed intercropping to be advantageous, with high Land Equivalent Ratio values. Soybean genotypes showed drastic decreases in yield when intercropped. Asgrow A6520 soybean had the highest yield in intercropping. Sorghum #3 (California IO80H40) and soybean #3 (Rillito) were chosen as the most appropriate genotypes for intercropping, for the environmental conditions of the study.

No description available.

No description available.