Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 223-235).

Thesis (Ph.D.)--University of Delaware, 2009. / Principal faculty advisor: Lynn Snyder-Mackler, Dept. of Physical Therapy. Includes bibliographical references.

This is an interdisciplinary research project in which the methods of biomechanical and design engineering are focused upon a problem in orthopaedics. The anterior cruciate ligament (ACL) is the major ligament in the knee and is often torn during athletic competition as well as every day activity. The ACL is made up of two functional bundles, which help to stabilize the knee. Until recently, ACL reconstruction only replaced one of these bundles; however, research shows that both bundles should be replaced to more fully restore normal knee functionality. The aim of the research was, therefore, to evaluate the mechanical aspects of the double-tunnel-double-bundle ACL reconstruction technique. The research was directed towards designing a new and improved surgical device to improve ACL reconstruction: The current study used a computational model and experimental testing to explore the mechanical parameters of the tendon graft and knee bones to investigate the effects of double tunnel drilling in tibia and femur during ACL reconstruction. The thesis presents the findings of research into three aspects of double-tunnel-double-bundle ACL reconstruction. The first aspect of the study involves clinical and computational analysis of a single-tunnel-singlebundle (SB) ACL reconstructed knee with a double-tunnel-double-bundle (DB) ACL reconstructed example. The study tried to show the advantage of the DB technique over the conventional SB technique. The anatomical geometries of both SB and DB examples were used to create a finite element model and investigate the relative merits of single and double tunnelling, the variations of graft pretension, and tunnel placement on bone stress. The experimental and computational results of both methods were compared and discussed. The second study investigated whether tripling a tendon when using suspensory fixation provides inferior graft strength and a greater cyclical elongation than a doubled tendon graft with suspensory fixation. The tensile stress was found to be lower in the third strand than in the doubled portion. The study was focussed on the mechanical assessment of two different methods of tripling tendons when using suspensory fixation. The third aspect of the study focussed on the design of a new device for fixation of the femoral tripled tendon graft in DB ACL reconstruction technique. The study describes a series of designs and prototypes that were iteratively developed and experimentally tested, leading to a novel tripled tendon graft device. The function of the new device was compared with the conventional methods and tested with a number of animal tendons and bones. The new device with a tripled tendon graft resulted in higher pull-out strength and less graft elongation than that seen using a conventional tripling method.

An in vitro model system utilizing de-mineralized periodontium tissues was developed for investigating the molecular controls involved in the spatial deposition of minerals on collagenous tissues. Preferential mineral deposition was observed when the de-mineralized tissue sections were incubated in solutions containing a supersaturation of calcium and phosphate ions with respect to hydroxyapatite (HA). Energy dispersive X-Ray (EDX) analysis demonstrated that these minerals are likely to be octacalcium phosphate (OCP) or dicalcium phosphate dihydrate (DCPD); further characterization with a secondary technique is required to draw a more definitive conclusion. The role of collagen fibrils in mineralization was tested by removing all the non-collagenous components from the tissue sections with proteolytic enzymes and exposing them to similar mineralization conditions as the control samples. A substantially less amount of minerals were formed in these samples; this correlates well with the hypothesis in the literature that collagen fibrils alone cannot initiate mineral formation.

An in vitro model system utilizing de-mineralized periodontium tissues was developed for investigating the molecular controls involved in the spatial deposition of minerals on collagenous tissues. Preferential mineral deposition was observed when the de-mineralized tissue sections were incubated in solutions containing a supersaturation of calcium and phosphate ions with respect to hydroxyapatite (HA). Energy dispersive X-Ray (EDX) analysis demonstrated that these minerals are likely to be octacalcium phosphate (OCP) or dicalcium phosphate dihydrate (DCPD); further characterization with a secondary technique is required to draw a more definitive conclusion. The role of collagen fibrils in mineralization was tested by removing all the non-collagenous components from the tissue sections with proteolytic enzymes and exposing them to similar mineralization conditions as the control samples. A substantially less amount of minerals were formed in these samples; this correlates well with the hypothesis in the literature that collagen fibrils alone cannot initiate mineral formation.

The purpose of this investigation was to document the influence of the anterior cruciate ligament (ACL) in controlling the dynamic interaction between the femur and tibia. Twenty subjects were initially selected to participate based on the results of arthroscopic and proprioceptive tests which established the presence of a uni-lateral, third degree rupture of the ACL. A spline function established the best fitting curve of the instant center (IC) coordinates obtained throughout the ROM. Displacement variables were extrapolated from joint rotation profiles generated from these coordinates. Abnormal displacement migrations of the IC parameter were demonstrated to occur in extension. Migration displacements evaluated between 30$ sp circ$ and 0$ sp circ$ shifted anteriorly 5.07 $ pm$ 1.86 mm for intact knees and 6.97 $ pm$ 2.06 mm for involved knees. This was determined to be significant at the 0.05 level of confidence and correlated well with clinical findings. Mean migration displacements evaluated in flexion (between 100$ sp circ$ and 70$ sp circ)$ were not found to differ significantly between knee conditions.

No description available.

Movement variability that occurs while performing repetitions of any particular motion can be due to errors in one’s ability to select the required parameters for executing the movement. However, it has been suggested that an optimal amount of variability exists in a healthy system providing adaptability to varying situations while producing negligible errors. Investigation of the consistency of movement variability in a system may provide information regarding joint control and integrity since the functionality of a system can be disrupted by injury. Rupture of the anterior cruciate ligament is associated with deficits in knee joint stability and mobility, as well as altered movement patterns. There is value in developing a simple clinical test that can measure knee joint stability and evaluate the degree of knee damage and rate of progress during rehabilitation. The main objectives were to investigate the normal range of variability during repeated knee extensions in healthy subjects and subjects with a reconstructed anterior cruciate ligament, and to evaluate the reliability of the method. There were 30 participants in the control group and 8 in the subject group. All were physically active a minimum of 2-3 times per week for a total of 2-3 hours. Mean variance and mean correlation were used to evaluate variability in this study. Change in mean variance and mean correlation, standard error of measurement and intra-class correlation coefficient were used to evaluate reliability. The results showed the range of values for movement variability in control and subject groups. Standard error of measurement indicated mean correlation (1.31% to 2.38%) was more reliable than mean variance (21.80% to 54.87%). Mean variance and mean correlation significantly increased with speed, and mean correlation was significantly higher for dominant legs during trials at 70 beats per minute. / Thesis (Master, Kinesiology & Health Studies) -- Queen's University, 2014-06-02 12:18:42.802

This study used vital bone markers to investigate mineralized tissue adaptation in the periodontium of rats after a hypothermic insult to their maxillary first molars. This hypothermic insult has been shown in previous studies to induce aseptic root resorption with variable effects on ankylosis. A secondary objective was to assess the pulpal changes that occurred concurrent with the changes in the periodontium. Four groups of 7, eight-week old male Sprague Dawley rats were assigned to be euthanased at the day 7, 14, 21 and 28 observation periods. At day 0, 4 groups of 6 rats were subject to a single 20 minute application of dry ice on their maxillary right first molar. The remaining 1 rat within each group did not receive the dry ice. All rats were given 2 sequential bone labels, calcein 5mg/kg and alizarin red 30mg/kg, administered intraperitoneally 8 days apart. The timing of the labels was such that all rats were euthanased 2 days after the last label. The rat maxillae were fixed in ethanol and embedded undecalcified in methylmethacrylate. Ten micrometre coronal sections were obtained through the furcation of the first molars with three of each group of ten consecutive sections being unstained, stained with von Kossa/ hematoxylin and eosin counterstain, or decalcified and stained with hematoxylin and eosin, respectively. Unstained sections were viewed under fluorescence, while transmitted light microscopy was used for the other sections. Mineral apposition rates along the bone, root and pulpal surfaces as well as periodontal ligament width were measured using histomorphometry. Semiquantitative measurements of the resorptive surfaces within the periodontium were also noted. Multivariate and negative binomial regression statistical analyses were used to identify influencing variables. A focal pattern of ankylosis was observed at days 14 and 21 in 3 rats and was not seen at day 28. In both the treated and control teeth, appositional activity was greatest along bone and least along the root surface. Mineral tissue apposition rates along the bone and root surface displayed an initial spike during day 14 but declined to levels of the control teeth by day 28. A longer time lag was observed with appositional activity in the pulp which also displayed a declining trend towards the control teeth values by day 28. Resorption levels along the root surface continued to remain significantly (p<0.0001) elevated. The significantly (p<0.0001) wider periodontal ligament width in the treated molars showed a declining trend towards that of the control teeth by day 28. There was a temporary disturbance of mineralized tissue adaptation on the bone and root surfaces with a recovery of cellular vitality within the periodontium and pulp and a trend towards homeostasis of the periodontal ligament width. The null hypotheses that a single prolonged thermal insult on a rat has no effect on mineralized tissue adaptation within the periodontium and pulp chamber and that the periodontal ligament width within the interradicular region does not change in response to thermal trauma induced by the present study were rejected. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1345386 / Thesis (D.Clin.Dent) - University of Adelaide, School of Dentistry, 2008

Thesis (MAppSc in Physiotherapy)--University of South Australia, 1995