Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-27T00:25:31Z No. of bitstreams: 1 VERS?O FINAL DISSERTA??O-Luma Passos.pdf: 2866580 bytes, checksum: 631bf5534fde43f51e60719229aabc72 (MD5) / Made available in DSpace on 2016-09-27T00:25:31Z (GMT). No. of bitstreams: 1 VERS?O FINAL DISSERTA??O-Luma Passos.pdf: 2866580 bytes, checksum: 631bf5534fde43f51e60719229aabc72 (MD5) Previous issue date: 2015-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / The genus Lippia (Verbenaceae) is characterized by having species with medicinal and aromatic properties, its economic potential aims to the production of essential oils with important biological and pharmacological activities. Even with all known diversity and potential, few species are the target of agronomic studies. Considering the propagation as the first step in the domestication of a species and the influence of external factors on essential oil production, this study aimed to evaluate the effect of cutting type and auxin concentrations in the vegetative propagation of the species L. insignis, L. lasiocalycina and L. thymoides, as well as different types of fertilization and harvest season in production, and content and chemical composition of the essential oil. In propagation tests two types of cuttings were used (apical and middle) and five IBA concentrations (0, 250, 500, 750 and 1000 mg L-1). In cultivation three forms of fertilization were tested (control, manure and manure + NPK 10:10:10) and two harvest seasons (210 and 360 days after planting). The essential oils are extracted from dry leaves by the hydrodistillation method by Clevenger apparatus, and chemical composition determined by gas chromatography with flame ionization detector (GC / FID) coupled to mass spectrometer (GC / MS). It was concluded that apical cuttings are the most suitable for the propagation of L. insignis and both apical and middle cuttings for L. thymoides and L. lasiocalycina; treatment with IBA is not necessary in the propagation by cuttings of these species; chemical fertilizers (NPK) associated with cattle manure was the best treatment in growth and biomass production of the species L. lasiocalycina and L. thymoides, while for L. insignis to the control treatment was the best; pruning, followed by the second growing season (regrowth) is recommended in the cultivation of the studied species, aiming at the production of essential oil; ?-myrcene, limonene and E-ocimenone are the major components of L. insignis; ?-myrcene and E-ocimenone the major components of L. lasiocalycina and E-caryophyllene and caryophyllene oxide the major components of L. thymoides; fertilization and harvest season promote quantitative variations in the chemical composition of essential oils; the species L. insignis and L. thymoides are the most promising for essential oil production in the conditions of Feira de Santana, Bahia, Brazil. / O g?nero Lippia (Verbenaceae) ? caracterizado por apresentar esp?cies com propriedades medicinais e arom?ticas, sendo seu potencial econ?mico voltado para a produ??o de ?leos essenciais com importantes atividades biol?gicas e farmacol?gicas. Mesmo com toda diversidade e potencial conhecidos, poucas esp?cies est?o sendo alvo de estudos agron?micos. Considerando a propaga??o como o primeiro passo na domestica??o de uma esp?cie e a influ?ncia dos fatores externos na produ??o de ?leo essencial, o presente trabalho teve como objetivo avaliar o efeito do tipo de estaca e concentra??es de auxina na propaga??o vegetativa das esp?cies L. insignis, L. lasiocalycina e L. thymoides, bem como de diferentes tipos de aduba??o e ?pocas de colheita na produ??o, teor e composi??o qu?mica do ?leo essencial. Nos testes de propaga??o foram utilizados dois tipos de estacas (apical e mediana) e cinco concentra??es de AIB (0, 250, 500, 750 e 1000 mg L-1). No cultivo foram testadas tr?s formas de aduba??o (testemunha, esterco bovino e esterco + NPK 10:10:10) e duas ?pocas de colheita (210 e 360 dias ap?s o plantio). Os ?leos essenciais foram extra?dos de folhas secas pelo m?todo de hidrodestila??o por aparelho de Clevenger e a composi??o qu?mica determinada por cromatografia gasosa com detector de ioniza??o em chama (CG/DIC) acoplado a espectr?metro de massas (CG/EM). Foi poss?vel concluir que estacas apicais s?o as mais indicadas na propaga??o de L. insignis e tanto as estacas apicais quanto medianas para L. thymoides e L. lasiocalycina; o tratamento com AIB n?o ? necess?rio na propaga??o por estaquia dessas esp?cies; a aduba??o mineral (NPK) associado a esterco bovino foi o melhor tratamento no crescimento e produ??o de biomassa das esp?cies L. lasiocalycina e L. thymoides, j? para L. insignis o tratamento testemunha foi o melhor; a poda, seguida da segunda ?poca de cultivo (rebrota) ? recomend?vel no cultivo das esp?cies estudadas, visando ? produ??o de ?leo essencial; ?-mirceno, limoneno e E-ocimenona s?o os componentes majorit?rios de L. insignis; ?-mirceno e E-ocimenona os componentes majorit?rios de L. lasiocalycina e E-cariofileno e ?xido de cariofileno os componentes majorit?rios de L. thymoides; a aduba??o e a ?poca de colheita promovem varia??es quantitativas na composi??o qu?mica dos ?leos essenciais; as esp?cies L. insignis e L. thymoides s?o as mais promissoras na produ??o de ?leo essencial nas condi??es de Feira de Santana, Bahia, Brasil.