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Isolation and characterisation of phages infecting gram positive food bacteriaLee, Wan-Jing January 2008 (has links)
Bacteriophage (phage), virus of bacteria, has been proposed as a mean to inactivate bacteria that are pathogens of humans. Applied prophylatically to food, phage might decrease the numbers of potential pathogens we ingest. Much active research on using the phages of bacteria to control Gram negative foodborne pathogens are described in the literatures, but comparatively little research describes the phages of Gram positive bacteria and their use as biocontrol agents on food. In this work, previous undescribed phages, able to infect Bacillus cereus and Listeria monocytogenes, were isolated from soil and ruminants faecal material, respectively. As the first step in assessing their potential as biocontrol agents, the isolated phages were purified, concentrated and characterised (albeit to different degrees). The Bacillus phages had a narrow host range while the Listeria phages had a broad host range. Listeria phages also infected L. monocytogenes 2000/47, a strain which recurs in New Zealand clinical cases. Both Bacillus and Listeria phages appeared to be of the Myoviridae family judging by their structure in electron micrographs. The Bacillus FWLBc1 and FWLBc2 phages were lytic phages with a latent period of 106 and 102 min at 37°C, and an average burst size of 322 and 300 phages per infected cell, respectively. Moreover, they both had genomes of approximately 134 kb. All newly isolated and characterized phages were chloroform resistant and survived storage better at 4°C than at room or freezing temperatures. Bacillus phages significantly reduced the bacterial population in mashed potatoes within 24 h at room temperature, when applied at a phage to host ratio of 1000. Listeria phages rapidly inactivated the host population to a low optical density. The findings of this thesis will add to the current knowledge of phages in the context of various environmental conditions for different bacteria and will demonstrate the potential of phages as food safety biocontrol agents.
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Influence of environmental factors and the host's susceptibility on the development of listeria monocytogenes infection in the guinea pig modelPang, Hoan-Jen E. January 2007 (has links)
Thesis (Ph. D.)--Rutgers University, 2007. / "Graduate Program in Food Science." Includes bibliographical references (p. 89-91).
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Attachment of Listeria monocytogenes to austenitic stainless steelMai, Le Phuong Tam. January 2006 (has links) (PDF)
Dissertation (Ph.D.)--Auburn University, 2006. / Abstract. Vita. Includes bibliographic references.
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Listeria monocytogenes, zoonotic exposure, rural residency, and preventionKersting, Ann L., January 2008 (has links)
Thesis (Ph. D.)--Ohio State University, 2008. / Title from first page of PDF file. Includes bibliographical references (p. 142-164).
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Exploring the Listeria monocytogenes secretome : identification and functional characterization of novel virulence factors and secretion systems /Port, Gary C. January 2007 (has links)
Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 170-192).
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Molecular characterization of fur and transcriptional profiling of fur- and iron- regulated gene expression in Listeria monocytogenesLedala, Nagender. Jayaswal, Radheshyam K. January 2007 (has links)
Thesis (Ph. D.)--Illinois State University, 2007. / Title from title page screen, viewed on July 16, 2008. Dissertation Committee: Radheshyam K. Jayaswal (chair), Brian J. Wilkinson, Anthony J. Otsuka, Wade A. Nichols, Laura A. Vogel. Includes bibliographical references and abstract. Also available in print.
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Influence of immunoenhancement by dietary vitamin E supplementation on the development of Listeria monocytogenes infection in aged and young guinea pigsWu, Wen-Hsua. January 2009 (has links)
Thesis (M.S.)--Rutgers University, 2009. / "Graduate Program in Food Science." Includes bibliographical references (p. 58-64).
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Development of a novel FRET-based biosensor for detection of foodborne pathogensKo, Sungho, January 2004 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2004. / Typescript. Vita. Includes bibliographical references (leaves 115-120). Also available on the Internet.
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Development of a novel FRET-based biosensor for detection of foodborne pathogens /Ko, Sungho, January 2004 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2004. / Typescript. Vita. Includes bibliographical references (leaves 115-120). Also available on the Internet.
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Ultra high pressure inactivation of Saccharomyces cerevisiae and Listeria innocua on fruitChauvin, Maite Andrea, January 2004 (has links) (PDF)
Thesis (M.S. in food science)--Washington State University. / Includes bibliographical references.
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