Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T15:33:12Z No. of bitstreams: 2 lorena_leao_jesus.pdf: 826450 bytes, checksum: 3a10a7ec4a4676a5ccb38e308ea778fb (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T15:33:34Z (GMT) No. of bitstreams: 2 lorena_leao_jesus.pdf: 826450 bytes, checksum: 3a10a7ec4a4676a5ccb38e308ea778fb (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T15:34:18Z (GMT) No. of bitstreams: 2 lorena_leao_jesus.pdf: 826450 bytes, checksum: 3a10a7ec4a4676a5ccb38e308ea778fb (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2014-12-22T15:34:18Z (GMT). No. of bitstreams: 2 lorena_leao_jesus.pdf: 826450 bytes, checksum: 3a10a7ec4a4676a5ccb38e308ea778fb (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2014 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / A cultura do gergelim, Sesamum indicum L., por possuir cerca de 50% de teor de ?leo em suas sementes, surge como uma alternativa para produ??o de biocombust?veis podendo competir com outras oleaginosas. No entanto, a utiliza??o de sementes de alta qualidade ? de fundamental import?ncia para o estabelecimento da cultura. Para a cultura do gergelim n?o existem metodologias adequadas para avalia??o da qualidade fisiol?gica de suas sementes. Objetivou-se com esta pesquisa adequar as metodologias dos testes de condutividade el?trica, envelhecimento acelerado, lixivia??o de pot?ssio e tetraz?lio para avalia??o da qualidade de sementes de gergelim. Foram testadas as metodologias do teste de condutividade el?trica utilizando embebi??o por 4, 8, 12, 16, 20 e 24 horas e combina??es n?mero de sementes/volume de ?gua deionizada (25/25 mL; 25/50 mL; 25/75 mL; 50/25 mL; 50/50 mL e 50/75 mL), para o teste de envelhecimento acelerado testou-se a metodologia tradicional com ?gua e com solu??o saturada de NaCl por 0, 24, 48, 72 e 96 horas, para o teste de lixivia??o de pot?ssio, embebi??o por 30, 60, 90, 120, 150 e 180 minutos, nas combina??es n?mero de sementes/volume de ?gua destilada (25/25 mL; 25/50 mL; 50/25 mL; 50/50 mL) e no teste de tetraz?lio foram testadas as concentra??es de 0,075%; 0,5% e 1,0% de solu??o de tetraz?lio a 25 ?C, por 3, 6, 8, 12, 18 e 24 horas. Concluiu-se que no teste de condutividade el?trica o per?odo de embebi??o de 4 horas e a combina??o de 25 sementes com 75 mL e 50 sementes com 75 mL ? eficiente para a distin??o da qualidade fisiol?gica de sementes de gergelim. Para o teste de envelhecimento acelerado o per?odo de envelhecimento de 48 horas pelo m?todo tradicional ou 72 horas e 96 horas com solu??o saturada de NaCl permite separar as cultivares de gergelim em diferentes n?veis de vigor. No teste de lixivia??o de pot?ssio a combina??o 25 sementes, 25 mL de ?gua e 60 minutos ou 90 minutos de embebi??o ? adequada para avalia??o do potencial fisiol?gico das sementes. A embebi??o das sementes sobre papel por 60 minutos, seguida de corte longitudinal e imers?o em solu??o de tetraz?lio na concentra??o de 0,075% por 12 horas e 1,0% por 3 horas ? eficiente na avalia??o da viabilidade das sementes de gergelim. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT The culture of, Sesamum indicum L., possess a circa of 50% content of oil within the seeds, surge as an alternative for the production of biofuels able to compete with other oilseeds. However, the utilization of high quality seeds is of fundamental importance for the establishment of cultivation. For the cultivation of sesame seeds, there exists no adapted methodologies for the evaluation of physical quality of the seeds. The objective of this study is to adapt a methodology to tests of electrical conductivity, accelerated aging, tetrazolium and potassium leachate to evaluate the quality of the seeds. The methodology of the electrical conductivity test were tested using imbibition for 4, 8, 12, 16, 20 and 24 hours and a number of combinations of seeds/volume in deionized water (25/25 mL; 25/50 mL; 25/75 mL; 50/25 mL; 50/50 mL e 50/75 mL), for the accelerated aging test tested the traditional methodology applied was with water and saturated solution NaCl for 0, 24, 48, 72 and 96 hours, for potassium leachate, imbibition for 30, 60, 90, 120 and 180 minutes with a number of combinations of seeds/volume in distilled water (25/25 mL; 25/50 mL; 50/25 mL and 50/50 mL) and tetrazolium test concentrations were 0.075%, 0.5% and 1.0% of the tetrazolium solution, at 25 ?C, for 3, 6, 8, 12, 18 and 24 hours. The conclusion of the electrical conductivity test demonstrate that the period of imbibition for 4 hours and the combination of 25 seeds with 75 mL of water and 50 seeds with 75 mL of water is efficient for the distinction of the physiological quality of the sesame seeds. For the test of accelerated aging, the period of aging for 48 hours by the traditional method or 72 hours and 96 hours with the saturated solution NaCl, permits the separation of the cultivars of sesame seeds in different levels of vigor. In the potassium leachate test, a combination of 25 seeds with 25 mL of water for 60 minutes or 90 minutes of imbibition is adequate for the evaluation of the physiological potential of the seeds. The imbibition of the seeds on paper for 60 minutes, following a longitudinal cut and immersion in the tetrazolium solution with a concentration of 0.075% for 12 hours and 1.0% for 3 hours is efficient in the evaluation of the viability of the sesame seeds.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-06-27T17:39:00Z No. of bitstreams: 1 MariaLuizaDeSouzaMedeiros_DISSERT.pdf: 778297 bytes, checksum: 3012a0a9e0e06a0f632900e02f88e311 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-06-29T20:16:49Z (GMT) No. of bitstreams: 1 MariaLuizaDeSouzaMedeiros_DISSERT.pdf: 778297 bytes, checksum: 3012a0a9e0e06a0f632900e02f88e311 (MD5) / Made available in DSpace on 2016-06-29T20:16:49Z (GMT). No. of bitstreams: 1 MariaLuizaDeSouzaMedeiros_DISSERT.pdf: 778297 bytes, checksum: 3012a0a9e0e06a0f632900e02f88e311 (MD5) Previous issue date: 2015-12-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A avalia??o do vigor de sementes ? um fator importante para a detec??o de lotes de sementes de alta qualidade, de modo que o desenvolvimento de procedimentos para avaliar o potencial fisiol?gico tem sido importante ferramenta dentro de programas de controle de qualidade de sementes. Nesse sentido, este trabalho teve como objetivo adequar as metodologias dos testes de envelhecimento acelerado, condutividade el?trica e lixivia??o de pot?ssio para a avalia??o do vigor de sementes de Moringa oleifera LAM.. Para tanto, quatro lotes de sementes foram submetidos aos testes de germina??o, emerg?ncia de pl?ntula, ?ndice de velocidade de emerg?ncia, primeira contagem de emerg?ncia, comprimento e massa seca de pl?ntulas e teste frio, para a sua caracteriza??o fisiol?gica, al?m dos testes de envelhecimento acelerado, condutividade el?trica e lixivia??o de pot?ssio. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repeti??es de 50 sementes, e as m?dias comparadas pelo teste de Tukey a 5% de probabilidade, sendo realizada a an?lise de regress?o polinomial no teste de condutividade el?trica. Para o teste de envelhecimento acelerado, foram estudados os per?odos de envelhecimento de 12, 24 e 72 horas, a 40, 42 e 45 ?C. Para o teste de condutividade el?trica, utilizou-se a temperatura de 25 ?C por per?odos de 4, 8, 12, 16 e 24 horas de imers?o, em 75 e 125 mL de ?gua destilada, utilizando-se 25 e 50 sementes e, para o teste de lixivia??o de pot?ssio, utilizou-se amostras de 25 e 50 sementes, colocadas em copos pl?sticos contendo 70 e 100 mL de ?gua destilada ? 25 ?C por per?odos de 1, 2, 3, 4, 5 e 6 horas. A partir dos resultados obtidos, pode-se inferir que as metodologias que melhor se ajustaram para o teste de envelhecimento acelerado em sementes de moringa foram a temperatura de 40 ?C por 12 e 72 horas, 42 ?C a 72 horas e 45 ?C a 24 horas. Para o teste de condutividade el?trica em sementes de moringa, a combina??o de 50 sementes em 75 mL de ?gua destilada por per?odo de embebi??o de 4 horas e, 50 sementes em 125 ml por 4 horas foram eficiente para a diferencia??o dos lotes de sementes de moringa quanto ao vigor e, para o teste de lixivia??o de pot?ssio em sementes de moringa, a combina??o de 50 sementes /100 mL de ?gua destilada permitiu a separa??o dos lotes em quatro n?veis de vigor, a partir de 2 horas de imers?o, mostrando-se promissor na avalia??o da qualidade de sementes de moringa. / The evaluation of seed vigor is an important factor for detection of lots of high quality seeds, so that development of procedures to evaluate the physiological potential has been an important tool in quality control programs seeds. In this sense the study aimed to adapt the methodologies of accelerated aging, electrical conductivity and potassium leaching to evaluate Moringa oleifera seed vigor LAM.. Therefore, four lots of moringa seeds were subjected to the germination tests, seedling emergence, speed of emergence index, emergence first count, length and dry mass of seedlings and cold test for their physiological characterization, in addition to accelerated aging, electrical conductivity and potassium leaching. The experimental design was completely randomized with four replications of 50 seeds and the means compared by Tukey test at 5% probability. For accelerated aging the periods were studied aging 12, 24 and 72 hours at 40, 42 and 45?C. For the electrical conductivity test was used to a temperature of 25?C for periods of 4, 8, 12, 16 and 24 hours of immersion in 75 to 125 mL of distilled water, using 25 to 50 seeds, and for potassium leaching test samples were used 25 to 50 seeds, placed in plastic cups containing 70 and 100 mL of distilled water at 25?C for periods of 1, 2, 3, 4, 5 and 6 hours. From the results obtained, it can be inferred that the methods best fit for the accelerated aging test Moringa seeds were a temperature of 40?C for 12 to 72 hours, 42?C 72 hours 45?C 24 hours . In the electrical conductivity test Moringa seeds, the combination of 50 seeds in 75 mL distilled water for a period of immersion of 4 hours and 50 seeds in 125 mL of 4 hours were efficient for the differentiation of lots of Moringa seeds as to vigor and for potassium leaching test moringa seeds, the combination of 50 seeds in 100mL of distilled water allowed the separation of lots of four levels of vigor, at 2 hours of immersion, showing promise in evaluate the quality of moringa seeds.

Made available in DSpace on 2014-12-17T14:17:54Z (GMT). No. of bitstreams: 1 MirellaCSA_DISSERT.pdf: 648930 bytes, checksum: 06627ea14cbf2543775d82cecd4ee6ec (MD5) Previous issue date: 2014-03-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Mimosa caesalpiniaefolia Benth. is a forest species of the Mimosaceae family, recommended for recovery of degraded areas. The evaluation of vigor by biochemical tests have been an important tool in the control of seed quality programs, and the electrical conductivity and potassium leaching the most efficient in the verifying the physiological potential. The objective, therefore, to adjust the methodology of the electrical conductivity test for seeds of M. caesalpiniaefolia, for then compare the efficiency of this test with the potassium in the evaluation of seed vigor of different lots of seeds M. caesalpiniaefolia. To test the adequacy of the electrical conductivity were used different combinations of temperatures , 25 ?C and 30 ?C, number of seeds , 25 and 50, periods of imbibition , 4 , 8 , 12 , 16 and 24 hours , and volumes deionized water, 50 mL and 75mL. For potassium leaching test, which was conducted from the results achieved by the methodology of the adequacy of the electrical conductivity test, to compare the efficiency of both tests , in the classification of seeds at different levels of vigor, and the period 4 hours also evaluated because the potassium leaching test can be more efficient in the shortest time . The best combination obtained in experiment of electrical conductivity is 25 seeds soaked in 50 mL deionized or distilled water for 8 hours at a temperature of 30 ? C. Data were subjected to analysis of variance, the means were compared with each other by F tests and Tukey at 5 % probability, and when necessary polynomial regression analysis was performed. The electrical conductivity test performed at period eight hour proved to be more efficient in the separation of seed lots M. caesalpiniaefolia at different levels of vigor compared to the potassium test / Mimosa caesalpiniaefolia Benth. ? uma esp?cie florestal pertencente ? fam?lia Mimosaceae, recomendada para recomposi??o de ?reas degradadas. A avalia??o do vigor por meio de testes bioqu?micos tem sido importante ferramenta dentro de programas de controle de qualidade de sementes, sendo os testes de condutividade el?trica e lixivia??o de pot?ssio um dos mais utilizados na verifica??o do potencial fisiol?gico. Objetivou-se, portanto, adequar a metodologia do teste de condutividade el?trica para sementes de M. caesalpiniaefolia, para ent?o comparar a efici?ncia deste teste com o de lixivia??o de pot?ssio na avalia??o de vigor das sementes dos diferentes lotes de M. caesalpiniaefolia. Para o teste de adequa??o da condutividade el?trica foram utilizadas diferentes combina??es de temperaturas, 25 e 30 ?C, de n?mero de sementes, 25 e 50, de per?odos de tempo de embebi??o, 4, 8, 12, 16 e 24 horas, e de volumes de ?gua deionizada, 50 e 75 mL. Para o teste de lixivia??o de pot?ssio, que foi realizado a partir dos resultados alcan?ados pela metodologia da adequa??o do teste de condutividade el?trica, p?de-se comparar a efici?ncia de ambos os testes, na classifica??o dos lotes das sementes em diferentes n?veis de vigor, sendo o per?odo de 4 horas tamb?m avaliado devido o teste de lixivia??o de pot?ssio poder ser mais eficiente em menor espa?o de tempo. A melhor combina??o obtida no experimento de adequa??o da condutividade el?trica ? a combina??o de 25 sementes embebidas em 50 mL de ?gua deionizada durante 8 horas a uma temperatura de 30 ?C. Os dados foram submetidos ? an?lise de vari?ncia, as m?dias comparadas entre si pelos testes F e de Tukey a 5% de probabilidade, e quando necess?rio foi realizada an?lise de regress?o polinomial. O teste de condutividade el?trica realizado no per?odo de oito horas mostra ser mais eficiente na separa??o de lotes de sementes de M. caesalpiniaefolia em diferentes n?veis de vigor comparativamente ao teste de lixivia??o de pot?ssio