Birth weight (BW) (n =1277) and weaning weight (WW) (n = 1090) of calves, pregnancy rate (PR) (n = 1386), calf crop born (CCB) (n = 1386), calf crop weaned (CCW) (n = 1294), cow’s weight at palpation (CW) (n = 1474) and cow body condition score (BCS) (n = 1473) were evaluated from 1994 to 2006 in 143 F1 cows sired by Brahman (B), Boran (Bo) and Tuli (T) bulls and born to Angus and Hereford cows. Mouth scores (MS) (n = 139) were assigned to the remaining cows in 2004 and 2005. Fixed effects included sire breed of cow, dam breed of cow, and calf’s birth year/age of cow; random effects included cow and sire of cow. BW and WW were evaluated using the same model and adding gender for both and age for WW. All two-way interactions were tested for significance. Calf’s birth year/age of dam was significant for all traits (P < 0.05) except WW. BW for calves out of F1 B, Bo and T bulls were 35.08, 34.76 and 34.87 kg, respectively, and were not different. WW differed (P < 0.05) for calves out of F1 B, Bo and T cows (235.87, 221.10 and 208.35 kg, respectively). PR (0.922, 0.955 and 0.936, respectively), CCB (0.881, 0.931, 0.890, respectively), CCW (0.848, 0.898 and 0.869, respectively), did not differ among F1 B, Bo and T cows. CW when cows were 8- or 9-year old were 600.78, 514.63 and 513.14 kg, respectively, for F1 B, Bo and T cows, with those sired by B being heaviest (P < 0.05). BCS for B-, Bo- and T-sired cows were 5.23, 5.48 and 5.18, respectively, with F1 Bo cows having highest scores. Higher MS (P < 0.05) were assigned to Bo and B-sired cows (0.95 and 0.94, respectively) compared to T-sired cows (0.78), when both broken and solid incisors were scored 1, and smooth scored 0. When both smooth and broken were scored 0, and solid were scored 1, higher scores were assigned to B- (0.53) compared to T-sired cows (0.24), the Bo-sired cows being intermediate. Higher reproductive rates were found for Boran-sired cows, but Brahman-sired cows weaned heavier calves.

A series of recent studies using Drosophila melanogaster suggest that while males may benefit from having access to many partners, female fitness is reduced by extended cohabitation and sexual interaction with males. Yet, even if repeated sexual interactions are harmful to females, limited male exposure will ultimately be detrimental due to sperm-depletion and infertility. Females are therefore expected to balance mating opportunities and sperm storage capacity to maximize lifetime reproductive success. I introduced extended mating deprivation as a selective pressure to experimentally evolve lines of D. melanogaster for characters related to mating and postcopulatory sexual selection. Evolution of the mate-deprived lines over several dozen generations demonstrated that restricted sexual access was indeed a potent selective pressure. I consistently found that when males were removed for an extended time period, female fitness declined substantially, suggesting that mate-deprivation over nine days was harmful. Under these conditions, selected-line males responded to mate-reduced conditions and demonstrated a 13% increase in reproductive success compared to controls. Experimental females had a 15% increase in fertility compared to controls. I investigated a series of developmental characteristics that may have been altered by the selection regime, and while there was some evidence of evolved change, these results were not consistent. Although the data at hand do not substantiate a detailed characterization, both sexes in the experimental populations demonstrated increased fitness after extended mate-deprivation, thus evolutionary change appears to have occurred via selection on one or both relevant male ejaculate characteristics: sperm number and survival, and factors affecting female late-life fertility. / Thesis (Master, Biology) -- Queen's University, 2008-04-28 23:05:42.835

clk-1 mutants of the nematode C. elegans have been phenotypically characterized in previous work and found to have decreased rates of development and of periodic adult behaviours, such as defecation, pumping and swimming (Wong et al., 1995). In this study, the defecation periods in older wild type and clk-1 mutant worms were found to be similar despite their differences in young adults. Transgenic strains which express high levels of clk-1 were characterized phenotypically. Over-expression of the clk-1 gene has no observed effect on physiological rates as measured conventionally in young adults but rather has an effect on defecation rates in an age-dependent manner. A proportion of ageing transgenic animals over-expressing clk-1 did not have decreased defecation period, as seen in ageing wild type and clk-1 mutants. This suggests that the action of CLK-1, which functions in controlling physiological rates, is reduced in older wild type worms. As previously characterized, clk-1 mutants have lengthened life spans compared to wild type (Wong et al., 1995). Consistent with this effect, we found transgenic strains with high clk-1 expression to have a shortened average life span. Furthermore, no effect on average life span was found in transgenic control strains in which clk-1 expression was not altered, confirming that the presence of the transgene and the phenotype marking its presence do not influence the observed effects in strains with high clk-1 expression. Together, the findings from both the studies on physiological rates and life span suggest that clk-1 plays a role in determining the rate at which worms age.

The Caenorhabditis elegans gene gro-1 belongs to the Clock group of genes. The four known genes making up this class are believed to be involved in a general mechanism acting to coordinate the time-dependent processes in the organism. Mutation of these genes alter the timing of many disparate processes. This results in the mean lengthening of embryonic and post-embryonic development, as well as in a lengthening of the periods of a number of adult behaviours including pharyngeal pumping, swimming and the defecation cycle. These mutants also exhibit a significantly increased life span. The gene gro-1 has been cloned and encodes a metazoan N6-(Delta2) isopentenyl PPi: tRNA isopentenyl transferase. In S. cerevisiae and bacteria this enzyme has been shown to modify tRNAs that code for codons begining with U. This modification consists of the isopentenylation of the adenine residue at position 37, adjacent to the anti-codon. Interestingly, gro-1 is the fifth member of an operon. Preliminary expression studies with GFP reporter constructs suggest that as in yeast, GRO-1 is expressed in the cytoplasm and mitochondria, as well as in the nucleus.

The gene clk-2 of C. elegans is required in both the germline and the soma, for subsequent embryonic viability, and for developmental and behavioural rates, respectively, clk-2 encodes a protein that is homologous to Tel2p in yeast, which is required for the telomere length regulation. It has been demonstrated that clk-2 affects telomere length also in worms and human cells. By now the exact biochemical function of CLK-2 is unknown. In order to shed light onto the function of the gene clk-2, a two-hybrid screen was carried out to identify the interactors of the protein CLK-2. A potential interactor of CLK-2, Y105C5B.19, was identified in the screen. Y105C5B.19 is a novel gene and does not have homologues in other species. Y105C5B.19 contains an MSP (major sperm protein) domain, therefore it is possible that it could be involved in the processes that regulate oocyte maturation, gonadal sheath contractions, or sperm mobility. Interestingly, given that clk-2 is required for subsequent embryogenesis at some point during a narrow time between the end of oocyte maturation and the 2-cell stage, it is tempting to speculate that the interaction between CLK-2 and Y105C5B.19 might be functionally relevant. The lethality of clk-2(qm37) mutants might result from delayed consequences of defects in ovulation and/or fertilization, and perhaps such defects could result from the disruption of the interaction between CLK-2 and Y105C5B.19. The amino acid substitution C772Y resulting from the clk-2(qm37) mutation was found to disrupt the interaction between CLK-2 and Y105C5B.19 in a two-hybrid assay, lending support to the idea that the interaction between CLK-2 and Y105C5B.19 takes place in vivo.

In an effort to find new genes involved in development and the biological timing, I carried out a new screen for viable maternal-effect mutations similar in protocol to the previous screen in which 24 such genes have been identified. I screened 10,600 genomes and isolated 6 slow-growing mutations and 5 behavioral and morphological mutations. Another maternal-effect slow-growing mutation is isolated from a screen for both maternal-effect and non maternal-effect slow development mutations. Genetic mapping suggests that none of the seven slow growing mutations corresponds to previously identified genes, so seven new clk genes (clk-4, clk-5, clk-6, clk-7 clk-8, clk-9, clk-10) have been identified. Because most identified clk genes (clk-2, clk-4 to clk-10 and gro-1) are defined by single allele, we believe that these genes have not yet been saturated. Mutants of seven new clk genes have typical Clk phenotypes: a mean lengthening of embryonic development, postembryonic development, defecation cycle and life span. As the screening procedure did not involve any measure of life span, it is suggested that slow life is sufficient for long life. As expected, all seven mutations can be maternally rescued.

The nematode worm Caenorhabditis elegans has become a model system for the analysis of the genetics of aging. Previously, mutations in four genes, age-1, clk-1, daf-2 and daf-28 had been shown to lengthen adult life span. Based on the molecular genetic analysis of these genes, the sole function of the dauer genes age-1, daf-2 and possibly daf-28 is to regulate the activity of the forkhead-like transcription factor daf-16. daf-16 may determine life span by regulating the transcription of genes that are necessary for resistance to stresses, especially oxidative stress. Mutations in clk-1 affect behavioral and developmental timing as well as increasing mean and maximum life span. I show that mutations in the genes clk-2, clk-3 and gro-1 affect many of the same processes as clk-1 and that these four genes interact to determine the length of development and adult life span. These four Clock genes lengthen life span in a manner that is distinct from that of the dauer genes. clk-1 has been cloned and has been implicated in the regulation of metabolism. This suggests that Clock mutants may live long because they have reduced metabolic rates. I also show that mutations in 7 genes that affect feeding behavior, eat-1, eat-2, eat-3, eat-6, eat-13, eat-18 and unc-26 lengthen life span. This effect is presumably due to reduced caloric intake (caloric restriction) which has been shown to lengthen the life span of a wide variety of animals. eat-2 lengthens life span by a mechanism that is distinct from that of the dauer mutants but may be similar to that of the Clock mutants. This suggests that caloric restriction may also reduce metabolic rates, possibly through down-regulation of the Clock genes. These results indicate that life span in C. elegans is a polygenic trait, influenced by many different physiological processes. The study of genes that affect aging in C. elegans provides support for the antagonistic pleiotropy and free radical theories of aging.

This dissertation is arranged in three parts which address issues concerning death and immortality. These issues all revolve around the temporal limit imposed by death. I have endeavoured to pose some questions which have relevance to a society clutching onto traditional notions of an afterlife despite its headlong thrust into a technologically advanced future where ethical confusions abound. In the first chapter I examine the metaphysics of the soul because postulating an afterlife is the conventional response to death anxiety. I have noticed that many people cling to hope of immortality without having any understanding of the metaphysical architecture of this hope. Consequently, from a philosophical perspective, I contrast arguments on the soul by Plato, Aristotle, Aquinas and Descartes. The second chapter emerges from a fascination with Epicurus's theory of living and, derivatively, of dying. It involves a comparison with (1) Aristotle's distinction between energeia (actuality) and kinesis (movement), and (2) the sybaritic hedonism of the Cyrenaics. Although the theme of this chapter is pleasure, it will be seen that pleasure lies embedded in the living project and is an important component in the drive for survival. In the third chapter I reflect on the meaning of life in conjunction with length of time lived. Here I compare the meaninglessness experienced by Leo Tolstoy (because his life is too short) and the meaninglessness experienced by the fictional character Elena Makropulos (because her life is too long). The Makropulos "case", both a play by Karel Capek and an opera by Leos Janacek, was introduced into the philosophical world by Bernard Williams.

This dissertation is arranged in three parts which address issues concerning death and immortality. These issues all revolve around the temporal limit imposed by death. I have endeavoured to pose some questions which have relevance to a society clutching onto traditional notions of an afterlife despite its headlong thrust into a technologically advanced future where ethical confusions abound. In the first chapter I examine the metaphysics of the soul because postulating an afterlife is the conventional response to death anxiety. I have noticed that many people cling to hope of immortality without having any understanding of the metaphysical architecture of this hope. Consequently, from a philosophical perspective, I contrast arguments on the soul by Plato, Aristotle, Aquinas and Descartes. The second chapter emerges from a fascination with Epicurus's theory of living and, derivatively, of dying. It involves a comparison with (1) Aristotle's distinction between energeia (actuality) and kinesis (movement), and (2) the sybaritic hedonism of the Cyrenaics. Although the theme of this chapter is pleasure, it will be seen that pleasure lies embedded in the living project and is an important component in the drive for survival. In the third chapter I reflect on the meaning of life in conjunction with length of time lived. Here I compare the meaninglessness experienced by Leo Tolstoy (because his life is too short) and the meaninglessness experienced by the fictional character Elena Makropulos (because her life is too long). The Makropulos "case", both a play by Karel Capek and an opera by Leos Janacek, was introduced into the philosophical world by Bernard Williams.

This dissertation is arranged in three parts which address issues concerning death and immortality. These issues all revolve around the temporal limit imposed by death. I have endeavoured to pose some questions which have relevance to a society clutching onto traditional notions of an afterlife despite its headlong thrust into a technologically advanced future where ethical confusions abound. In the first chapter I examine the metaphysics of the soul because postulating an afterlife is the conventional response to death anxiety. I have noticed that many people cling to hope of immortality without having any understanding of the metaphysical architecture of this hope. Consequently, from a philosophical perspective, I contrast arguments on the soul by Plato, Aristotle, Aquinas and Descartes. The second chapter emerges from a fascination with Epicurus's theory of living and, derivatively, of dying. It involves a comparison with (1) Aristotle's distinction between energeia (actuality) and kinesis (movement), and (2) the sybaritic hedonism of the Cyrenaics. Although the theme of this chapter is pleasure, it will be seen that pleasure lies embedded in the living project and is an important component in the drive for survival. In the third chapter I reflect on the meaning of life in conjunction with length of time lived. Here I compare the meaninglessness experienced by Leo Tolstoy (because his life is too short) and the meaninglessness experienced by the fictional character Elena Makropulos (because her life is too long). The Makropulos "case", both a play by Karel Capek and an opera by Leos Janacek, was introduced into the philosophical world by Bernard Williams.