The rate of motility of lymphocytes from anemic mice of the WW[superscript V] genotype and their normal ++ littermates was determined in vitro by time-lapse cinephotomicrography. A comparison of their speeds on an isogenic kidney monolayer or feeder layer suggests that the speed of locomotion of lymphocytes from the WW[superscript V] mice may be somewhat reduced. If subsequently verified, this would suggest that cells produced in the lymphatic tissue show, in addition to the well known abnormalities in the erythrocytes, pigmented cells of the neural crest and the primordial germ cells, a further defect caused by mutation at the W locus. / Science, Faculty of / Zoology, Department of / Graduate

No description available.

No description available.

Cell image analysis of lymphocytes from (1) control and (2) killed coccidioides immitis arthrospore immunized guinea pigs was performed. Lymphocytes from peripheral blood and lymph nodes were obtained two and tree weeks after immunization from each group of animals. Histograms from peripheral lymphocytes taken at two weeks from immunized animals showed no significant difference from those of the control animals. A significant difference, however, was seen in the conturs of the histograms of the cells taken at three weeks from four of six immunized animals. These histograms extended into the higher optical density range when compared to histograms of control animals. Smaller nuclear areas were also found in the majority of three week peripheral cells. In material from the nodes of immunized animals two weeks after immunization, the ratio of densely staining to lightly staining cells reverses from 3:1 to .7:1. Samples taken from nodes three weeks after immunization show no such differences in their cellular composition between control and immunized animals.

Thesis (Ph. D.)--West Virginia University, 2004. / Title from document title page. Document formatted into pages; contains xi, 196 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (doctoral)--Københavns universitet.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (Ph. D.)--University of Missouri-Columbia, 2006. / Title from title screen of research.pdf file (viewed December 22, 2006). The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "May 2006" Includes bibliographical references.

Les amplificateurs (aussi appelés par le terme anglais enhancers) ont été initialement identifiés comme des séquences d'ADN agissant en cis qui augmentent la transcription d'une manière qui est indépendante de leur orientation et de leur distance par rapport au site d’initiation de la transcription. En outre, les gènes d'identité cellulaire sont souvent associés à des regroupements ou clusters d’enhancers, structures également appelées super-enhancers, censés assurer une régulation correcte de l'expression des gènes tout au long du développement et de la différenciation des cellules. Pour mieux comprendre la régulation des gènes à partir de ces réseaux régulateurs complexes, nous avons étudié la régulation du gène Ikzf1 qui code pour un facteur de transcription essentiel à la différenciation lymphoïde et également impliqué dans la leucémogenèse. En combinant différents types de données épigénomiques, nous avons privilégié l’étude d’un élément enhancer situé à 120 kb en amont du gène Ikzf1. Nous avons trouvé que la délétion de l'enhancer IkE120 entraine une réduction significative de l'ARNm d’Ikzf1. Cependant, nous avons observé que la transcription immature ainsi que l’usage des promoteurs et exons alternatifs d’Ikzf1 sont différemment affectée dans les cellules délitées par IKE120. Ces résultats semblent indiquer que l'élément IkE120 pourrait avoir des fonctions supplémentaires au-delà de la seule régulation de l'initiation de la transcription. / Enhancers were originally identified as cis-acting DNA sequences that increase transcription in a manner that is independent of their orientation and distance relative to the RNA start site. In addition, cell identity genes are often associated with cluster of enhancers, also termed super-enhancers, which are believed to ensure proper regulation of gene expression throughout cell development and differentiation. To better understand gene regulation based on these complex regulatory networks, we studied the regulation of the Ikzf1 gene which encoded for a lymphoid-specific transcription factor essential for lymphoid differentiation and also involved in leukemogenesis. By combining different epigenomics data sets we prioritize an enhancer element located 120 kb upstream the IKZF1 gene. We found that deletion of the E120 enhancer resulted in significant reduction of Ikzf1 mRNA. However, we observed that immature transcription, promoter and exon usage were differentially affected in the IKE120-deleted cells. The results indicated that E120 element might have additional functions over solely regulating transcription initiation. We suggest that expression of some tissue-specific and cell identity genes might, at least partially, be regulated at the level of mRNA maturation and that components of enhancer’s clusters are directly involved in this process.