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Geographic Information Systems (GIS) a computerized mapping system for capturing, storing, retrieving, analysing, and displaying spatial data (the type of data that has a geographic location). It is one of the fastest growing high tech fields and has been widely used in my areas where analysis of spatial referenced data is needed. In this paper we developed and implemented a GIS-based personalized Travel Planning application. Our goal is to provide a map based information system that gives recommendation information (e.g. closeby sights, must-see sights) to travellers according to their destinations, locations and preferences. Furthermore, the TP application was developed as a client-side service under the Tourist Information Provider System. It runs on client machines and uses the spatial referenced data stored at the TIP server-side database.

Thesis (Ph. D.)--Texas State University-San Marcos, 2006. / Vita. Appendices: leaves 162-247. Includes bibliographical references (leaves 248-262).

Integration of two or more genomic maps provides a higher density of markers and greater genome coverage than can be obtained with the resources available for a single mapping study. Map integration is important in any species for which an annotated complete genome sequence is not available. For organisms currently being sequenced, a pre-sequence integrated map is essential to provide the "backbone" for assembly of the sequence. Map integration also facilitates the identification and resolution of discrepancies among different maps; mapping of QTLs, ESTs, and BACs; and positioning of candidate genes. However, the inconsistencies in markers and populations used in individual mapping studies limit our ability to fully integrate the available data. By concentrating on marker orders rather than marker distances, one can join together published map data to include a majority of markers with the best estimate of their order in the genome. In this study, a comprehensive reference map was constructed from 28 published cotton AD genome maps. The output reference map contains 7,424 markers and represents over 93% of the combined mapping information from the 28 individual AD genome genetic maps. This study applied the use of bioinformatics and computational biology in cotton genome mapping integration. The output will be stored and displayed through CottonDB (http://www.cottondb.org), a public cotton genome database.

This thesis was made for Cybercom in Ostersund. They wanted an evaluation of HTML5 cross platform applications which studied HTML5 features as well as JavaScript libraries and frameworks. The resources put into mobile application development can be reduced bymaking applications that can work on all platforms instead of only natively. These types ofapplications are called cross platform applications and can be developed with specic tools.One way to develop cross platform applications is by using HTML5, which can either beused as a web application or packed into native applications using plug-ins. The purpose ofthis thesis was to create a mobile web app that can save maps to be used oine. The workwas done by evaluating frameworks for web applications. Frameworks that provide userinterface elements and features similar to those in native applications, and libraries that render maps served by map servers. Development environments for web development were also tested and evaluated. The results of the research and development were documented experience, and a HTML5 application that shows a map, has GPS functionality and can beused oine.

Integration of two or more genomic maps provides a higher density of markers and greater genome coverage than can be obtained with the resources available for a single mapping study. Map integration is important in any species for which an annotated complete genome sequence is not available. For organisms currently being sequenced, a pre-sequence integrated map is essential to provide the "backbone" for assembly of the sequence. Map integration also facilitates the identification and resolution of discrepancies among different maps; mapping of QTLs, ESTs, and BACs; and positioning of candidate genes. However, the inconsistencies in markers and populations used in individual mapping studies limit our ability to fully integrate the available data. By concentrating on marker orders rather than marker distances, one can join together published map data to include a majority of markers with the best estimate of their order in the genome. In this study, a comprehensive reference map was constructed from 28 published cotton AD genome maps. The output reference map contains 7,424 markers and represents over 93% of the combined mapping information from the 28 individual AD genome genetic maps. This study applied the use of bioinformatics and computational biology in cotton genome mapping integration. The output will be stored and displayed through CottonDB (http://www.cottondb.org), a public cotton genome database.

More and more corporations deem knowledge not only as their nuclear competitiveness when the face challenge, but also as an intangible asset. Each corporation has different demand and urgency for knowledge management. To realize its demand, you must have enough information first to understand its own knowledge. Therefore, some scholars advance a concept to use ¡§Knowledge Map¡¨ to depict the knowledge a corporation owns and thus help the corporation to find out the distribution and intensity of its knowledge. This study submits a set of methods to construct knowledge map and establishes dimensions of knowledge through literature review and experts¡¦ suggestions. Using this category and knowledge measurement index, a questionnaire evaluation distribution and intensity of a corporation¡¦s knowledge has been established. Academically, this questionnaire can be a reference for relevant studies about constructing knowledge map. In practice, corporations can also use this evaluation tool to conduct knowledge measurement, management and enhancement. In addition, this study has chosen five kinds of industries to conduct a survey and experimental research with this questionnaire. The result finds that there are different distributions and intensity of knowledge to certain extends between knowledge-intensive industries and non-knowledge-intensive industries. This could be a reference for further related study.

Stallion fertility is of significant economic importance in the multibillion dollar equine industry. Presently, the underlying genetic causes of infertility in stallions are unknown. Analysis of the human genome has shown that in more than 25% of cases, male infertility is associated with deletions/rearrangements in the Y chromosome. Presently there is no gene map for the Y chromosome in the horse. Therefore, the primary aim of this study is to build a detailed physical map of the chromosome with a long-term aim to identify and analyze Y-specific factors affecting fertility in stallions. To materialize this, we constructed the first radiation hybrid and FISH map of the euchromatic region of the horse Y chromosome. This basic map was used to obtain Y-specific BAC clones that provided new STS markers from the end sequences. Chromosome walking provided 73 BACs comprising 7 contigs that were built across the euchromatic region using 124 markers for content mapping. The results were validated by restriction fingerprinting and Fiber FISH. The map is presently the most informative among the domestic species and second to only human and mouse Y chromosome maps. The construction of this map will pave the way for isolation and functional characterization of genes critical for normal male fertility and reproduction and will in the future lead to the development of a diagnostic test to facilitate early identification of deletions/rearrangements on the Y chromosome of potentially affected foals/stallions. The second part of the study comprised the first extended investigation to assess genetic variation in the horse Y chromosome. Approximately 4.5Mb of the euchromatic region was screened for polymorphic microsatellite markers. Of the 27 markers that were characterized and screened for polymorphism in 14 breeds of the domestic horse and eight extant equids, only one was polymorphic in the domestic horse, suggesting a low level of genetic variation on the chromosome. However, 21 of the markers showed noteworthy variation (on average four alleles/marker) among the eight equids. These markers will be vital in future studies aimed at elucidating the genetic relationships between the various equids through phylogenetic analysis.

Due to their role in cellular signaling, mitogen activated protein (MAP) kinases represent targets of pharmaceutical interest. Most MAP kinase inhibitors target the highly conserved ATP binding site. This conservation promotes cross-reactivity and toxicities that may limit their potential as drugs. These drawbacks motivate the search for non-ATP competitive inhibitors with acceptable specificity and potency and also drive efforts to understand MAPK regulation. We applied a virtual screening (VS) workflow to discover novel scaffolds for ATP-independent JNK (C-Jun N-terminal Kinase) inhibitors targeting the JNK-JIP (JNK Interacting Protein) interaction. (-)-Zuonin A was identified as an inhibitor of JNK, exhibiting 100-fold selectivity for the JNKs over other MAP kinases. (-)-Zuonin A was characterized extensively both in vitro and in cell-based assays. The JNK2 isoform has been reported to regulate breast cancer cell migration. Accordingly, we engineered a JNK2-selective peptide inhibitor. Peptides derived from the JIP scaffolds linked to the cell-penetrating peptide TAT are used widely to investigate JNK-mediated signaling events without exibiiting isoform selectivity. Herein, Several JIP-based peptide sequences were designed and tested. A JIP sequence connected through a flexible linker to either the N-terminus of an inverted TAT sequence [mathematical equation], or to a poly-arginine sequence [mathematical equation] enabled the potent inhibition of JNK2 (IC₅₀~90 nM) with 10-fold selectivity over JNK1 and JNK3. Both peptides revealed a potent ability to inhibit the induction of JNK activation and c-Jun phosphorylation in HEK293 cells treated with anisomycin, and inhibited the migration of Polyoma Middle-T Antigen Mammary Tumor (P[subscript y]VMT) cells through the selective inhibition of JNK2. ERK2 dimerization has been reported to regulate its nuclear translocation and signaling. Our analysis using light scattering, analytical ultracentrifugation and NMR provide strong evidence that ERK2 is monomeric under physiological conditions. / text