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Fabricação de microambientes para crescimento celular utilizando polimerização via absorção de dois fótons / Fabrication of micro-environments to study cell growth by two-photon absorption polymerizationSalas, Oriana Ines Avila 25 November 2013 (has links)
Neste trabalho, demonstramos a fabricação de microambientes tridimensionais para investigar o crescimento celular. Inicialmente, desenvolvemos um sistema de microfabricação que utiliza fotopolimerização via absorção de dois fótons, com o qual se pode fabricar um conjunto de microestruturas com formas e espaçamentos pré-determinados. Este sistema de fabricação utiliza pulsos de femtossegundos, provenientes de um laser de Ti:safira operando em 790 nm. A intensidade destes pulsos é alta o bastante para induzir a absorção de dois fótons no fotoiniciador, o qual é responsável por promover a polimerização em uma resina acrílica. A natureza não linear da absorção de dois fótons confina a excitação ao volume focal, permitindo a fabricação de estruturas tridimensionais com alta resolução espacial. Para a obtenção dos microambientes, foi necessário o desenvolvimento de um sistema opto-mecânico de movimentação, tanto do feixe quando do substrato da amostra. Com esta técnica, fabricamos microambientes compostos de estruturas com diferentes formas (paralelepípedos, cilindros e cones) e espaçamentos, os quais foram caracterizados através de microscopia óptica e eletrônica. Para demonstrar a viabilidade destes microambientes para a investigação do crescimento celular, estes foram utilizados para monitorar o desenvolvimento da célula Michigan Cancer Foundation-7 (MCF-7), uma linhagem celular de adenocarcinoma de mama que apresenta fenótipo tumoral amplamente utilizada como modelo de estudo para câncer de mama. Observamos, via microscopia óptica de transmissão e fluorescência, o desenvolvimento das células MCF-7 nos distintos microambientes. Nossos resultados indicam uma melhor aderência e, portanto, desenvolvimento celular nas microestruturas cilíndricas. Observamos ainda uma maior densidade de células nos microambientes com estruturas separadas de 12 µm, a qual diminui com o aumento do espaçamento, de tal forma que para o microambiente com 30 µm, por exemplo, poucas células são observadas. Portanto, nossos resultados demonstram que os microambientes desenvolvidos são viáveis para estudos mais aprofundados em biologia celular, com potenciais aplicações em engenharia de tecido. / In this work we have demonstrated the fabrication of tridimensional microenvironments for the investigation of cell growth. Initially we have developed a two-photon absorption photopolymerization microfabrication system, which allows producing a set of microstructures with predetermined forms and spacing. This fabrication system uses femtosecond pulses from a Ti: sapphire laser operating at 790 nm. These pulses are intense enough to induce two-photon absorption by the photoinitiator, that is responsible for promoting the polymerization in an acrylic resin. The nonlinear nature of the two-photon absorption confines the excitation to the focal volume, allowing the fabrication of tridimensional structures with high spatial resolution. In order to obtain the microenvironments, it was necessary to develop a movement system for both the laser beam and the sample substrate. With this technique we have fabricated microenvironments composed by structures with different geometries (parallelepipeds, cylinders and cones) and spacing, which were characterized by optical and scanning electron microscopes. To demonstrate the feasibility of the microenvironments for the investigation of cell growth, the samples were used to monitor de development of the Michigan Cancer Foundation-7 cell (MCF-7), a lineage of breast adenocarcinoma that has a tumoral phenotype and is highly used as a model in breast cancer studies. We have observed, through conventional optical and fluorescence microscopy, the growth of the MCF-7 cells in the various microenvironments. Our results indicate a better adhesion and, therefore, better development of cells on the cylindrical microstructures. We also observe a higher cell density in the microenvironments with microstructures having a spacing of 12 µm, which reduces as the distance between microstructures increases, in such a way that for the microenvironment with 30 µm spacing, for example, just a few cells are observed. Thus, our results demonstrate that the produced microenvironments are applicable in deeper studies in microbiology, with potential application in tissue engineering.
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Expression of sigma receptors in human cancer cell lines and effects of novel sigma-2 ligands on their proliferationAbbas, Haider January 2018 (has links)
Sigma receptors originally thought to be an opioid receptor is now categorized as a distinct class of receptor. There are two main subtypes, the sigma-1 receptor and an uncharacterised binding site, named the sigma-2 binding site. The presence of the sigma-2 binding site shows high correlation with proliferation of cells and is associated with cancer. I have categorized sigma-1 and sigma-2 binding sites in 11 human tumour cell lines. I have demonstrated that tumour cell lines from a range of tissues express both sigma-1 and sigma-2 binding sites. One exception is the MCF7 breast cancer cell line, which lacks sigma-1 receptors. I show that the quantitation of sigma-2 binding sites using the "masking" protocols are flawed, significantly overestimating levels of sigma-2 binding sites. I propose novel protocols to determine levels of sigma-1 receptors and sigma-2 binding sites in cell lines and tissue. Using radioligand binding assays in MCF7 cells, I have characterised novel sigma-2 ligands. These ligands are simple ammonium salts containing a single nitrogen atom. They are simpler than the previously recognised pharmacophore for the sigma-2 site. I have shown that these simple ammonium salts show graded affinity for the sigma-2 binding site. The highest affinity ligands were dihexylammonium (pKi 7.58) and dioctylammonium (pKi 7.9). I have used these ammonium salts and previously characterised ligands to determine sigma-2 binding site biology. I have shown that the biological activity of these drugs is related neither to their hydrophobicity nor their ability to effect calcium signalling in cells. I propose that the Hill slope of binding is inversely related to the efficacy of a ligand to inhibit metabolic activity of cancer cells. Furthermore, I offer an explanation as to why concentrations of sigma-2 ligands far higher than their determined binding affinities are required to inhibit metabolic activity.
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Fabricação de microambientes para crescimento celular utilizando polimerização via absorção de dois fótons / Fabrication of micro-environments to study cell growth by two-photon absorption polymerizationOriana Ines Avila Salas 25 November 2013 (has links)
Neste trabalho, demonstramos a fabricação de microambientes tridimensionais para investigar o crescimento celular. Inicialmente, desenvolvemos um sistema de microfabricação que utiliza fotopolimerização via absorção de dois fótons, com o qual se pode fabricar um conjunto de microestruturas com formas e espaçamentos pré-determinados. Este sistema de fabricação utiliza pulsos de femtossegundos, provenientes de um laser de Ti:safira operando em 790 nm. A intensidade destes pulsos é alta o bastante para induzir a absorção de dois fótons no fotoiniciador, o qual é responsável por promover a polimerização em uma resina acrílica. A natureza não linear da absorção de dois fótons confina a excitação ao volume focal, permitindo a fabricação de estruturas tridimensionais com alta resolução espacial. Para a obtenção dos microambientes, foi necessário o desenvolvimento de um sistema opto-mecânico de movimentação, tanto do feixe quando do substrato da amostra. Com esta técnica, fabricamos microambientes compostos de estruturas com diferentes formas (paralelepípedos, cilindros e cones) e espaçamentos, os quais foram caracterizados através de microscopia óptica e eletrônica. Para demonstrar a viabilidade destes microambientes para a investigação do crescimento celular, estes foram utilizados para monitorar o desenvolvimento da célula Michigan Cancer Foundation-7 (MCF-7), uma linhagem celular de adenocarcinoma de mama que apresenta fenótipo tumoral amplamente utilizada como modelo de estudo para câncer de mama. Observamos, via microscopia óptica de transmissão e fluorescência, o desenvolvimento das células MCF-7 nos distintos microambientes. Nossos resultados indicam uma melhor aderência e, portanto, desenvolvimento celular nas microestruturas cilíndricas. Observamos ainda uma maior densidade de células nos microambientes com estruturas separadas de 12 µm, a qual diminui com o aumento do espaçamento, de tal forma que para o microambiente com 30 µm, por exemplo, poucas células são observadas. Portanto, nossos resultados demonstram que os microambientes desenvolvidos são viáveis para estudos mais aprofundados em biologia celular, com potenciais aplicações em engenharia de tecido. / In this work we have demonstrated the fabrication of tridimensional microenvironments for the investigation of cell growth. Initially we have developed a two-photon absorption photopolymerization microfabrication system, which allows producing a set of microstructures with predetermined forms and spacing. This fabrication system uses femtosecond pulses from a Ti: sapphire laser operating at 790 nm. These pulses are intense enough to induce two-photon absorption by the photoinitiator, that is responsible for promoting the polymerization in an acrylic resin. The nonlinear nature of the two-photon absorption confines the excitation to the focal volume, allowing the fabrication of tridimensional structures with high spatial resolution. In order to obtain the microenvironments, it was necessary to develop a movement system for both the laser beam and the sample substrate. With this technique we have fabricated microenvironments composed by structures with different geometries (parallelepipeds, cylinders and cones) and spacing, which were characterized by optical and scanning electron microscopes. To demonstrate the feasibility of the microenvironments for the investigation of cell growth, the samples were used to monitor de development of the Michigan Cancer Foundation-7 cell (MCF-7), a lineage of breast adenocarcinoma that has a tumoral phenotype and is highly used as a model in breast cancer studies. We have observed, through conventional optical and fluorescence microscopy, the growth of the MCF-7 cells in the various microenvironments. Our results indicate a better adhesion and, therefore, better development of cells on the cylindrical microstructures. We also observe a higher cell density in the microenvironments with microstructures having a spacing of 12 µm, which reduces as the distance between microstructures increases, in such a way that for the microenvironment with 30 µm spacing, for example, just a few cells are observed. Thus, our results demonstrate that the produced microenvironments are applicable in deeper studies in microbiology, with potential application in tissue engineering.
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Characterizing substances into pharmacological classes using theirmorphological and metabolic profilesNygård, Emma January 2015 (has links)
Treatment of cancers has been improved and new findings in research communities areconstantly found, but there are still many questions about how to treat these complex diseases.One way to treat cancer is to expose cancer cells to drugs that kill the cancer cells to a largerextent than the normal cell from the same as well as other tissue types. Different drugcompounds have diverse molecular effects on the cancer cells and to evaluate them, studies ondifferent cell lines were performed.Experiments were performed to study morphological and metabolic changes on treatedcells. Morphological changes in growing populations of MCF-7 cells and MCF-10A cellswere studied by using a phase contrast video microscopy (IncuCyte) image analysis. Changesin levels of metabolites and proteins were analyzed using two different mass spectrometricmethods. Hierarchical clustering was used to study the relationship between the collectedspectra and the most outstanding subgroup (cluster) was a set of compounds related toestrogens.There were apparent morphological differences between the two different cell lines, bothwhen untreated and after induction of apoptosis. This study shows that, when examining themetabolic patterns, there are tendencies among the substances studied to form clustersaccording their pharmacological classes. Although more studies have to be performed in thisarea it has been showed that there are possibilities to determine which pharmacological class asubstance belongs to by examining the morphological and metabolic patterns.
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