No description available.

Populations of aerobic heterotrophic bacteria, niycelial fungi and yeasts occurring in the production of barley malt were examined by plating on agar media and by scanning electron microscopy. There was an increase in the total number of micro-organisms during germination of barley, although populations declined after kilning. Bacteria dominated numerically in all samples, with progressively lower populations of yeasts and filamentous fungi. There was no obvious pattern of spatial distribution of micro-organisms On/in the samples, although there appeared to be high populations of bacteria and fungal hyphae on the inner surface of kernels. The dominant groups of aerobic heterotrophic bacteria were presumptively identified as Alcaligenes sp., Arthrobacter globiformis, Clavibacter iranicuin, Erwinia herbicola, Lactobacillus spp. and Pseudomonas fluorescens. The principal filainentous fungi were Alternaria alternata, Aspergillus glaucus group, Cladosporium macrocarpum, Epicoccum purpurascens, Fusarium avenaceum, Geotrichum candidum and Penicillium spp. The yeasts isolated most frequently were Candida catenulata, Q. vini, Debaryomyces hansenii, Hansenula polyniorpha, Kloeckera apiculata, Rhodotorula nrncilaginosa, Sporobolomyces roseus and Trichosporon bei gelii. Representative bacteria, mycelial fungi and yeasts were examined for the ability to degrade 8-glucan, starch or arabinoxylan. Approximately 50% of the fungi, <50% of the bacteria and <25% of the yeasts degraded these substrates. A culture filtrate of nivale demonstrated marked ability to reduce -glucan viscometrically and colorimetrically. The organism also degraded raffinose and sucrose. In micro-malting experiments the addition of Fusarium nivale and Geotrichum candidum did not produce substantial changes in terms of the physical and chemical characteristics of the finished malts.

This Master thesis deals with the yield management evaluation and malting quality of chosen varieties of the spring malting barley in terms of half-working experiment in company "Kantor Václav - Agricultural businessman". There was chosen a stand of experiment and the values of yield management were observed, especially the number of plants, their offshoots and spikes per square unit, the quantity of grains in spikes, the weight of a thousand seeds and the yield. In case of the grain quality elements was rated a content of nitrogen substances, the drop above the sieve 2,8, 2,5, 2,2 mm and under the sieve 2,2 mm, then the content of amyloid, germination and its energy as well as the grain moisture. Among the rated varieties were placed: Malz, Sebastian, Laudis 550, Kangoo, Xanadu, Sunshine, Bojos, KWS Irina and Pionier. The results were filed in the tables and charts. The influence of variety on a different yield element was found out in the final comparison of all parameters. According to the aspect of the yield elements as well as the quality, the best values in this year taking experiment were reached by the Kangoo variety.

Licentiatavhandling (sammanfattning) Uppsala : Sveriges lantbruksuniversitet, 2006. / Härtill 2 uppsatser.

The purpose of this study is to explore the history and production processes of beer and examines the components that contribute to its brewing. The narrative will provide a timeline of the processes that are required to create the product of beer beginning with the agricultural products and following the brewing processes that result in beer as the finished product. I will also examine the business of agriculture and grain processing and will provide some historical perspectives of grain, beer, and malting. I will also consider whether the growing, processing, and malting of grains in the East Tennessee and Southwest Virginia Region is feasible as demonstrated through this research and reported findings.

Résumé : Chez lorge, la dégradation des polysaccarides des parois cellulaires et de lamidon est de première importance dans le contexte de sa valorisation brassicole. La présente recherche doctorale a été entreprise dans le but didentifier des marqueurs de sélection utilisables lors de lamélioration génétique de lorge de printemps pour ses qualités brassicoles. Des gènes candidats ont dabord été choisis sur base de leur expression dans les tissus de la graine en germination. Leur position cartographique a été déterminée et située par rapport à des marqueurs QTL connus de la qualité brassicole. Exploitant des variétés cultivées de qualités contrastées, une recherche de polymorphismes nucléotidiques au niveau de ces gènes a été réalisée. Tous les gènes savèrent polymorphes, certaines des variations de séquence conduisant à des modifications de la structure primaire de la protéine encodée. Plusieurs caractères technologiques ont été retenus et définis chez les variétés étudiées, en exploitant les données de la littérature : la teneur en extrait, la viscosité, la teneur en β-glucanes du mout, latténuation finale apparente et le pouvoir diastasique. Plusieurs caractères biochimiques ont été étudiés chez ces mêmes variétés et ont ciblé quatre activités enzymatiques impliquées dans la dégradation des polysaccharides pariétaux et de réserve. Le pouvoir discriminant et la simplicité du test dactivité amylase, ainsi que sa relation avec des critères technologiques importants, en font un test prometteur en vue de faciliter la sélection variétale. La relation entre les sites polymorphes de lADN et les caractères technologiques et biochimiques identifient une soixantaine de sites dont les polymorphismes semblent associés à au moins lun des caractères. Sur tous les sites polymorphes, treize sites sont choisis pour leur potentiel en tant que marqueurs de sélection. Ils distinguent en effet un allèle favorable et un allèle défavorable pour plus dun critère brassicole. Ces treize sites sont localisés sur des gènes de (1-3,1-4)-β-glucanase, un gène de (1-3)-β-glucanase, un gène de (1-4)-β-xylan endohydrolase et un gène dα-amylase. La mise en évidence de ces allèles peut être réalisée par des tests PCR simples (« allèles spécifiques ») et relativement peu coûteux, dont six ont été mis au point au terme de la recherche./ Summary: In barley, the degradation of cell wall polysaccharides and starch is of utmost importance for its malting quality. The aim of this thesis is the identification of selection markers useful in the breeding of spring Barley cultivars for improved malting quality. Candidate genes were first chosen based on their expression profile in tissues of germinating seeds. Their mapping positions were determined and compared with known QTLs for malting quality. Varieties with contrasted malting qualities were searched for DNA polymorphisms for each of these genes. All genes proved to be polymorphic, some of the sequence variations leading to changes in the primary structure of the encoded protein. Technological traits were chosen and used to characterize the varieties based on literature data: extract yield, viscosity, β-glucan content of the wort, final apparent attenuation and diastatic power. Biochemical traits were also investigated on the same varieties and focused on four enzyme activities implicated in cell wall polysaccharides and starch degradation. The Discriminant power and ease of the amylase test, as well as its relation with technological traits, make it a promising selection test in breeding programs. The relationship between the DNA polymorphisms and biochemical and technological traits reveals around sixty polymorphisms displaying apparent relationships with at least one trait. Thirteen out of them were chosen for their potential as selection markers. They are located on (1-3,1-4)-β-glucanase genes, on one (1-3)-β-glucanase gene, on one (1-4)-β-xylan endohydrolase gene and on one α-amylase gene. Detection of these alleles can be achieved by simple and inexpensive PCR tests ( allele specific), and six assay protocols have been set up at the completion of this research.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2007. / Härtill 4 uppsatser.

By purchasing malting barley the quality is evaluated according to standards, techniques, laboratory methods and qualitative indicators, which are set by norm. Evaluation of quality of barleycorn as a material for producing malt is used for setting the price, observing changes of the quality during storing etc. There were evaluated qualitative indicators of 13 sorts of malting barley, which were bought by company ZZN Pelhřimov between 2010-2014 from farmers, agricaltural cooperatives and companies and also self-employed farmers from the region Vysočina. These sorts were represented by different number of samples in connection with bought-up amount and number of supplies of each sort. Weighted averages were counted from results. Average figures of results of analysis were compared with the national average. Research institute of brewer´s and orchardist a.s. in Brno deals with monitoring of quality of malting barley in the Czech Republic and it evaluates annually ca. 500 harvest samples which were received by producers from all regions in the Czech Republic. The quality of malting barley showed a big variability in all parameters across all types and also in particular years. Malting quality is influenced by many factors especially by course of weather in each particular year. Limiting parameter of quality by purchasing malting barley was N- substances content in barleycorn. Germination capacity seems to be the most stable from all parameters. Lower figures of germination capacity were found only by winter types in connection with longer period of ripening after harvest.

The theses presents results of experiments with spring barley started in years 2017 and 2018. The experiments were put into practice at Mr Jiří Nepovím's (BSc) place in Opatovice near Světlá nad Sázavou. Laudis 550 was used as the experimental variety of spring barley and the objective of the experiment was to observe the increasing amount of nitrogen and its effect on the resulting quantity and quality. The experimental amounts of nitrogen were set to 60 kg.ha-1, 75 kg.ha-1, 90 kg.ha-1, 105 kg.ha-1 and 120 kg.ha-1. With the increasing amount of nitrogen the crop increased of 0,2 - 0,4 t.ha-1. The share of nitrogen substances in grain increased of 0,2-0,5 %.