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The aetiology of the proliferative enteropathiesMcOrist, S. January 1988 (has links)
The entezopathogenicity and antigens of Campylobacter-like organisms and Campylobacter app associated with the proliferative enteropathies were investigated. Two gnotobiotic pigs exposed orally to a filtered suspension of intestinal mucosa designated 284/86 from a naturally infected pig subsequently developed lesions of proliferative enteritis. Culture of the successful mucosal inoculum only revealed a moderate number of C. coli, however an apparently greater number of Campylobacter-like organisms was evident in smears of this inoculum. The pathogenesis of porcine proliferative enteritis was clearer from the results of this study. Ten days after infection, curved bacilli had colonised the ileal and large intestinal crypts. Attachment and entry of Campylobacter-like organisms into crypt enterocytes was also evident, with some proliferation of both bacteria within cells and of the enterocytes themselves. Twenty days after infection there was similar intracellular colonisation of bacteria and proliferative activity, although no luminal bacteria were evident. A moderate sub-acute inflammatory reaction was evident throughout. Conventional hamsters dosed with C. jejuni developed varying degrees of localised acute intestinal inflammation. Hamsters dosed with C. hyointestinalis or C. cola did not develop any lesions. Lesions of proliferative enteritis were detected in hamsters dosed with porcine tissue 284/86. Numerous intra-cytoplasmic Campylobacter-like organisms were detected within enterocytes in affected portions of intestine. Weanling hamsters this proved to be susceptible to the agent of porcine proliferative enteritis by cross-species transmission. Whole cell antigen preparations were made of various Camoylobacter sp. -Indirect immunofluorescence assays incorporating rabbit antisera to each Campylobacter sp gave specific endpoints for each antiserum of 1: 160 to 1: 320. Rabbit antisera prepared to Campylobacter-like organisms partly purified from proliferative enteritis mucosa, by a homogenisation and filtration technique, also gave specific reactions in this assay, up to 1: 610. Intracellular Campylobacter-like organisms were also compared in gel electrophoresis protein profiles and immunoblotting reactions to Campylobacter spp. The intracellular organisms tested had a distinctive protein profile dissimilar to the profiles of the known Campylobacter spp. In immunoblotting reactions, each of the Camoylobacter sp antisera reacted strongly with homologous antigens, but none reacted with Camcylobacter-like organisms prepared from lesions, except for a minor reaction seen with one serum. Similarly antisera to Campylobacter-like organisms showed a strong reaction to 25K to 27K components of homologous antigens, with only minor reactions to various other components of the cultivated Campylobacter app. Therefore it is likely that the intracellular Campylobacter-like organisms have a distinctive antigenic profile and that the 25 and 27K components are major antigenic components. Mouse monoclonal antibodies were produced that were apparently specific to the intracellular Camoylobacter-like organisms. Immunoblotting results showed that these antibodies only bound to a 251 to 27K outer membrane component present in the intracellular organisms. Reactions with this component could not be detected in assays with normal pig intestine, or Camoylobacter sp antigen. Restriction endonuclease digestion of Camoylobacter sp with Bgl II gave suitably resolved DNA fragments of between 2kb and 25kb. Patterns obtained with Bgl II digestion of Camoylobacter sp were dissimilar to those of Camoylobacter-like organisms, and each Camoylobacter sp had a characteristic distinct pattern. Digestion of DNA from porcine tissue samples with Bgl II produced a diffuse smear of fragmented DNA bands between 0.5 and19kb, with no recognizable "ladder" effect. The genome of the Carylobacter-like organisms within enterocytes in proliferative enteritis therefore is different to that of known Camoylobacter spp associated with the disease. This suggests that the differences in antigenic structures between these bacteria axe due to genetic differences. Only a limited number of strains were examined. Looking at the evidence provided by this study, the overall tenor of the results suggests that the intracellular organisms could be a separate, new species of Camp lobacter. If indeed the intracellular organisms are a single, new Cammylobacter PGS/ABST ecies, then a new name may be proposed, such as "C. intracellulare". Verifica nthis side Oni of the validity of such a proposal would require further DNA studies.
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Mammals of the Huntington Reservoir, Huntington and Wells Counties, IndianaReising, Johnny W. January 1974 (has links)
A survey of the Mammalian fauna of the Huntington Reservoir Area in Huntington and Wells Counties was conducted from March 1, 1973 through February 28, 1974. Collected specimens were identified to species, catalogued, and made into study skins and/or skulls prior to deposition in the Ball State University Mammal Collection. Data were recorded on body measurements, weights, reproductive condition, and skull measurements. These data were compared to previous mammal investigations in Indiana.The study revealed the presence of 27 species of mammals on or near the Huntington Reservoir Study Area. Twenty-one species were Collected and/or examined by the author, and the additional six species were collected and/or observed by Reservoir personnel.
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Reproductive energetics of the Tenrecidae (Mammalia: Insectivora)Stephenson, Peter James January 1991 (has links)
No description available.
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The life history of the narwhal, Monodon monoceros l., in the eastern Canadian arctic /Hay, Keith Alexander. January 1984 (has links)
The life history of the narwhal Monodon monoceros was studied utilizing animals captured by the Inuit in northern Baffin Island. Segregation by age and sex within this population is evident, with summering groups consisting of mature females with calves, immature and maturing males, and large mature males. The diet consists of arctic cod, shrimp, and squid during June and July, but feeding activity declines markedly during the open-water months of August and September. Growth layers in the unerupted teeth and periosteal zone of the mandible were found to be related to age but absolute rates of accumulation of these layers are uncertain. The maximum life span is estimated to be 40 to 50 years. Male narwhals, which mature sexually at lengths exceeding 390 cm and at 16-17 growth layers, display protracted maturation and a possible annual cycle of spermatogenesis. Females, which mature sexually at lengths exceeding 340 cm and at 12 growth layers, are seasonally polyoestrous, experiencing up to four consecutive ovulations during the breeding season. The gestation period is estimated to be 15.3 months. The season of conceptions is March to May and calving occurs during July and August. Since the lactation period exceeds 12 months, the interval between successive conceptions is usually three years, but about 20% of females conceive at the first breeding season following birth of their calves. The annual population birth rate is calculated to be about 0.07. The basic life history features of the narwhal are similar to those of other medium-sized toothed whales.
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The biology of mammalian spermatozoa in the oviductSmith, Todd Timothy January 1990 (has links)
Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1990. / Includes bibliographical references (leaves 97-104) / Microfiche. / ix, 109 leaves, bound ill. 29 cm
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Small mammal habitat associations in a fragmented agricultural landscapeHamrick, William Wall. January 2007 (has links) (PDF)
Thesis (M.S.)--Georgia Southern University, 2007. / "A thesis submitted to the Graduate Faculty of Georgia Southern University in partial fulfillment of the requirements for the degree Master of Science." In Biology, under the direction of C. Ray Chandler. ETD. Electronic version approved: December 2007. Includes bibliographical references (p. 68-74) and appendices.
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Mixodectidae, Microsyopidae, and the insectivore-primate transitionSzalay, Frederick S. January 1969 (has links)
Thesis(Ph.D.)--Columbia University. / Bibliography: p. 324-330.
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Genetic characterization of the Preimplantation Embryo Development (PED) gene using the congenic B6.K1 and B6.K2 strains of mice a dissertation /Byrne, Michael J., January 1900 (has links)
Thesis (Ph. D.)--Northeastern University, 2008. / Title from title page (viewed March 27, 2009). Graduate School of Arts and Sciences, Dept. of Biology. Includes bibliographical references (p. 140-156).
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The effects of vegetation, fire and other disturbance factors on small mammal ecology and conservationWilson, Barbara Anne. January 1990 (has links)
Thesis (Ph.D.)--Deakin University, Victoria, 1990. / Submitted to the Dept. of Biological Sciences of the School of Sciences, Deakin University. Appendices are separately published papers. Bibliography: leaves 274-302.
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Solving mammalian riddles : a reconstruction of the Tertiary and Quaternary distribution of mammals and their palaeoenvironments in island South-East Asia /Meijaard, Erik. January 2004 (has links)
Thesis (Ph.D.)--Australian National University, 2004.
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