The Best M.Phil Thesis in the Faculties of Dentistry, Engineering, Medicine and Science (University of Hong Kong), Li Ka Shing Prize,1995-1997 / published_or_final_version / Chemistry / Master / Master of Philosophy

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy

No description available.

<p>Artemisia Afra, a popular South African traditional herbal medicine is commonly administered as a tea infusion of the leaves. However, clinical trials proving it safety and efficacy are lacking mainly due to the absence of good quality dosage forms and credible placebos for the plant. The objectives of this study were to prepare a standardized preparation of the plant leaves and freeze-dried aqueous extract powder of the leaves, in a tea bag dosage form and to design and prepare credible placebos for these plant materials.</p>

<p>In South Africa, the crude aqueous extract from Hypoxis hemerocallidea is used by AIDS patients to treat opportunistic infections, such as tuberculosis. The rapid emergence of multidrug-resistant tuberculosis, and extreme drug resistant tuberculosis, in recent years, is a major threat to human health. The treatment of TB, nosocomial bacterial infections, and fungal infections is now a clinical challenge, especially in the immuno-compromised individual. There is a dire need for novel antibiotic alternatives with phytotherapies and plant-derived compounds as potentially promising alternatives. The main objective of this study was to investigate the antimycobacterial activity of Hypoxis hemerocallidea, a South African medicinal plant, using Mycobacterium smegmatis.</p>

Mini-dissertation submitted in partial compliance with the requirements for the Master's Degree in Technology: Homoeopathy, Durban University of Technology, 2007. / This research study was conducted to elucidate the total symptomatology that the plant Peucedanum galbanum in the thirtieth centesimal potency would produce on healthy individuals so that it may be prescribed according to the Law of Similiars. A further aim of the investigation was to compare this remedy to other remedies yielding the highest numerical values and total number of rubrics on repertorisation of the proving symptoms. Wagner (2007), in a concurrent study, analysed the proving results of Peucedanum galbanum in relation to the doctrine of signatures. It was hypothesised that Peucedanum galbanum 30CH would produce recognizable signs and symptoms in healthy provers, and that the comparative study of this remedy would highlight the differences and similarities between remedy symptoms and thus confusion as to indication is eliminated, plus a fuller understanding of the remedy and its relationship to other remedies is gained. / M

Submitted in fulfillment of the Master’s Degree in Homoeopathy, Department of Homoeopathy, Durban University of Technology, Durban, South Africa, 2016. / Aim The aim of this homoeopathic proving study was to determine and document the arising symptomatology of Acacia xanthophloea (Fever tree) in the potentised homoeopathic form, 30CH, and to provide this data for inclusion to the homoeopathic materia medica. The results of this proving study and comparative analysis to African traditional medicinal uses of this substance confirms the potential therapeutic value of the remedy. Methodology The homoeopathic drug proving of Acacia xanthophloea 30CH was conducted in the form of a double blinded placebo controlled study. The investigation consisted of a total of 30 provers divided equally between two researchers ((A. Gobind and G. Zondi). The sample was randomly divided into two groups in which 24 provers(80%) were assigned into the verum group and the remaining six provers (20%) were allocated to the placebo group. All provers were requested to record their daily symptoms on the physical, mental and emotional planes in their journals for one week prior to administration of the proving substance. This formed as a mode of control for the comparison of symptomatology for the pre-proving and post proving period. A thorough case history was taken and physical examination performed on each prover before the commencement of the proving and after the duration of the proving period. Each prover received a total of nine powders. Starting on day 8 of the study the provers consumed one powder three times a day for three days and documented their daily symptoms in a journal. The duration of the proving term was six weeks in total. During this interval the researcher maintained consistent contact with the provers. Upon completion of the proving period all journals were collected and the information contained within these journals was translated into the materia medica and repertory format. This facilitated the establishment of the remedy portrait of Acacia xanthophloea 30CH. A subsequent comparison between the symptomatology that materialised in the provers and the African traditional medicinal uses was duly conducted. Results An extensive range of symptoms was reported by the provers. The outstanding themes that emanated from this proving on the mental plane include anger, anxiety, aversion to company, cheerful, depression, irritability, mood swings, restlessness, tranquillity and stress. A broad range of headaches were described with some headaches being associated with the eye. There were many eye symptoms displayed by the provers which include itching, redness, burning sensation and pain. The stomach symptoms revealed marked increased thirst, changeable appetite, bloating, constipation and diarrhoea with watery stools.The female genitalia / sex indicated several symptoms ranging from painful menstruation, bleeding and copious blood flow.The greatest number of symptoms in a system was associated with extremities, producing the greatest number of rubrics in the repertory section. Dream themes depicted by the provers were especially visionary, about family and friends in addition to other themes. The correlation process between the homoeopathic drug proving of Acacia xanthophloea 30CH and the African traditional medicinal use of the substance brought several resemblances to light.There were clear similarities with the eye symptoms, gastrointestinal symptoms and headaches. Conclusion As hypothesised Acacia xanthophloea 30CH did produce distinctly observable signs and symptoms when administered to healthy provers. The symptoms that emerged during the proving provide evidence that an overlay exists between the remedy Acacia xanthphloea 30CH and the traditional use of the crude substance Acacia xanthophloea. The researcher proposes that further research should be conducted to determine the symptomatology of various homoeopathic potencies so that a complete image of the remedy Acacia xanthophloea 30CH can be established and the clinical applications can be broadened. / M

Submitted in fulfillment of the Master’s Degree in Homoeopathy, Department of Homoeopathy, Durban University of Technology, Durban, South Africa, 2016. / Aim The aim of this homoeopathic proving study was to determine and document the arising symptomatology of Acacia xanthophloea (Fever tree) in the potentised homoeopathic form, 30CH, and to provide this data for inclusion to the homoeopathic materia medica. The results of this proving study and comparative analysis to African traditional medicinal uses of this substance confirms the potential therapeutic value of the remedy. Methodology The homoeopathic drug proving of Acacia xanthophloea 30CH was conducted in the form of a double blinded placebo controlled study. The investigation consisted of a total of 30 provers divided equally between two researchers ((A. Gobind and G. Zondi). The sample was randomly divided into two groups in which 24 provers(80%) were assigned into the verum group and the remaining six provers (20%) were allocated to the placebo group. All provers were requested to record their daily symptoms on the physical, mental and emotional planes in their journals for one week prior to administration of the proving substance. This formed as a mode of control for the comparison of symptomatology for the pre-proving and post proving period. A thorough case history was taken and physical examination performed on each prover before the commencement of the proving and after the duration of the proving period. Each prover received a total of nine powders. Starting on day 8 of the study the provers consumed one powder three times a day for three days and documented their daily symptoms in a journal. The duration of the proving term was six weeks in total. During this interval the researcher maintained consistent contact with the provers. Upon completion of the proving period all journals were collected and the information contained within these journals was translated into the materia medica and repertory format. This facilitated the establishment of the remedy portrait of Acacia xanthophloea 30CH. A subsequent comparison between the symptomatology that materialised in the provers and the African traditional medicinal uses was duly conducted. Results An extensive range of symptoms was reported by the provers. The outstanding themes that emanated from this proving on the mental plane include anger, anxiety, aversion to company, cheerful, depression, irritability, mood swings, restlessness, tranquillity and stress. A broad range of headaches were described with some headaches being associated with the eye. There were many eye symptoms displayed by the provers which include itching, redness, burning sensation and pain. The stomach symptoms revealed marked increased thirst, changeable appetite, bloating, constipation and diarrhoea with watery stools.The female genitalia / sex indicated several symptoms ranging from painful menstruation, bleeding and copious blood flow.The greatest number of symptoms in a system was associated with extremities, producing the greatest number of rubrics in the repertory section. Dream themes depicted by the provers were especially visionary, about family and friends in addition to other themes. The correlation process between the homoeopathic drug proving of Acacia xanthophloea 30CH and the African traditional medicinal use of the substance brought several resemblances to light.There were clear similarities with the eye symptoms, gastrointestinal symptoms and headaches. Conclusion As hypothesised Acacia xanthophloea 30CH did produce distinctly observable signs and symptoms when administered to healthy provers. The symptoms that emerged during the proving provide evidence that an overlay exists between the remedy Acacia xanthphloea 30CH and the traditional use of the crude substance Acacia xanthophloea. The researcher proposes that further research should be conducted to determine the symptomatology of various homoeopathic potencies so that a complete image of the remedy Acacia xanthophloea 30CH can be established and the clinical applications can be broadened. / M

Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2016. / In Africa, herbal medicines are often used as primary treatments for a variety of ailments and diseases including HIV/AIDS and for HIV-related problems. In general, traditional medicines are not well researched scientifically in controlled studies, and are poorly regulated. Since the pharmacological effectiveness of natural products is affected by several native and foreign factors, studies on the variations of chemical composition and biological activity of these medicines are necessary. The processes of investigating plants to identify chemical substances are of great interest to natural product researchers because there is a need to discover new drugs for treating old and new diseases. These facts underscore an urgent need to develop new anti HIV and AIDS drugs with fewer or no side effects. Research into drug discovery and development using natural products is increasingly becoming better established. Marine organisms as a source of natural products delivered numerous novel compounds with multiple pharmacological properties. Natural products give endless opportunities for discovering novel compounds that can be used as drugs or backbones of drug leads. Manayi is a natural product that has been used to treat and manage people with HIV, but no scientific studies have been done to prove its efficacy on the HIV under controlled conditions. For the purposes of this study, cooked and uncooked Manayi product was evaluated for its efficacy on HIV in vitro. Manayi samples were collected in East London, Eastern Cape and sequentially extracted with hexane, chloroform, dichloromethane, butanol, methanol, and water as a series of increasingly polar solvents for its bioactive chemical constituents.

Thesis (PhD (Biomedical Sciences))--Cape Peninsula University of Technology, 2017. / The use of medicinal plants in the management of various health problems date back to the ancient times. However, only in recent years, researchers are starting to focus on the use of natural plant products as alternative treatment in disease control. Basella alba (Ba), commonly called Ceylon or Indian spinach is one of such medicinal plants, wildly cultivated and consumed mostly as vegetable. Studies have established many beneficial effects of Ba, including androgenic effects as well as antidiabetic effects which have been described in rats following oral administration of the leave extract. However, the actual mechanisms underlying the antihyperglyceamic effect of Ba have not been reported in any study and little or no research details are yet available on the potential beneficial effects of Ba in reproductive dysfunction resulting from diabetes mellitus. This study was aimed at investigating the mechanisms underlying the antidiabetic effect of Ba and the possibility of a role for the plant in correcting diabetic-induced reproductive dysfunctions in male Wistar rats. The first part of the study involved comparing of three different solvent extracts of Ba leaves namely ethyl acetate, methanolic and aqueous extracts for their antioxidant potentials, after which the aqueous extract was selected for further use in the experiments. Animal experimentation involved male rats (n=40) aged 8-10 weeks, randomly divided into four equal groups as follows: Healthy Control, Diabetic Control, Healthy Treatment and Diabetic Treatment. Diabetes was induced via a single intraperitoneal injection of streptozotocin (55mg/kg) and all animals subsequently received treatment via gavage (Rats in Control groups received 0.5ml/100g normal saline daily and treatment groups received 200mg/kg plant extract daily) for a period of four weeks. Fasting blood sugar and body weights were recorded weekly throughout the study. Animals were sacrificed upon completion of the treatment and blood samples and tissues collected for further analysis which included computer aided sperm analysis, Luminex® technology and enzyme-linked immunosorbent hormonal assays, inflammatory cytokine assays, analysis of oxidative stress markers and Histopathological analysis. The single intraperitoneal injection of a high streptozotocin dose resulted in hyperglycaemia, weight loss, subnormal sperm parameters, negative balance of inflammatory cytokines and endogenous antioxidants and degenerative changes in the pancreas, testes and epididymis as observed in the diabetic control rats. Oral administration with the aqueous extract of Ba for four weeks in diabetic treatment rats led to a significant reduction in blood sugar and improvement of sperm parameters by modulating the production of gonadal hormones, in vivo antioxidants and inflammatory cytokines. There was also significant recovery of normal islet histology and reduction in testicular and epididymal degeneration in the diabetic treatment rats when compared to their diabetic control counterparts. It was concluded from the findings of this study that the antidiabetic and profertility effects of Ba are largely dependent on the modulation of in vivo production of antioxidants, gonadal hormones and inflammatory cytokines, probably stimulated by one or more phytochemical component(s) that can be isolated in the aqueous extract of the plant.