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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

Enlightening Medicago truncatula transformation and shading GFP fluorescence

Zhou, Xin 01 November 2005 (has links)
Medicago truncatula (M. truncatula) has been proposed as a model legume for molecular and genetic studies of legumes. While many genetic resources have been developed for this model legume, genetic transformation of the M. truncatula line A17 proved to be a problem. A reproducible transformation method is described for M. truncatula A17. Procedures are detailed that yielded an average regeneration frequency of 35% for recovery of transgenic shoots from cotyledonary node explants. Previously, rooting of transgenic shoots of this line has proven difficult, but media and culture procedures are described that yielded an average frequency of 39% for root induction from 419 phosphinothricin-resistant shoots. Fertile M. truncatula A17 plants transgenic for 35S-GFP, phas-GUS or phas-GFP were obtained. The presence of transgenes was confirmed by expression of transgenes and by genomic DNA blots. Interestingly, although GUS and GFP driven by the phas promoter were very strongly and uniformly expressed in seed cotyledons of most transgenic M. truncatula lines, silencing of the GUS expression from the phas promoter was observed in several lines, indicating the occurrence of novel epigenetic events. The diminution of GFP fluorescence in transgenic M. truncatula occurs despite the presence of GFP transcript and protein. To evaluate the generality and causes of this phenomenon, fluorescence during leaf development from the same 35S-GFP transgene was compared in M. truncatula, rice and Arabidopsis. A substantial decrease in fluorescence early in the development of M. truncatula and rice leaves was found to correlate with chlorophyll accumulation. Several approaches showed that chlorophyll is causally involved in the loss of GFP fluorescence. Removal of chlorophyll from leaves of transgenic M. truncatula, rice or Arabidopsis through etiolation or by extraction with ethanol yielded up to a tenfold increase in fluorescence. Direct evidence that chlorophyll is implicated in the loss of fluorescence from GFP was obtained by mixing solutions of chlorophyll and GFP. At low concentration, fluorescence loss was fourfold greater for chlorophyll b than for chlorophyll a, reflecting their relative interference with GFP excitation and emission. Thus, substantial errors in estimating promoter activity from GFP fluorescence can occur if pigment interference is not considered.
12

Nodulation and nitrogen fixation in Medicago species at low pH / by Yulin Lestari.

Lestari, Yulin January 1993 (has links)
Bibliography: leaves 151-177. / xxiii, 177 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1993
13

Factors associated with variability in the growth of lucerne (Medicago sativa L)

Nikandrow, Alexander January 1979 (has links)
viii, 329 leaves : graphs, tables, photos, (1 col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1981
14

Factors associated with variability in the growth of lucerne (Medicago sativa L)

Nikandrow, Alexander January 1979 (has links)
viii, 329 leaves : graphs, tables, photos, (1 col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1981
15

Improving medic pastures in pasture-wheat rotations in the Mallee district of North-Western Victoria /

Latta, Roy A. January 1994 (has links) (PDF)
Thesis (M. Ag. Sc.)--University of Adelaide, Dept. of Plant Science, 1995? / Includes bibliographical references (leaves 115-130).
16

Variation in the sensitivity of nodulation and nitrogen fixation to nitrate in annual "Medicago" species /

Heidari Sharif Abad, Hossein. January 1994 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, (1995). / Includes bibliographical references (leaves 153-179).
17

The influence of soil, climatic and management factors on nitrogen accretion by annual Medicago species in a semi-arid environment of South Australia.

Dahmane, Ali Ben Kilani. January 1978 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Dept. of Agronomy, 1979.
18

Factors associated with variability in the growth of lucerne (Medicago sativa L).

Nikandrow, Alexander. January 1979 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Department of Plant Pathology, 1981.
19

Physical map between marker 8O7 and 146O17 on the Medicago truncatula linkage group 1 that contains the NIP gene

Lee, Yi-Ching. Dickstein, Rebecca, January 2007 (has links)
Thesis (M.S.)--University of North Texas, Dec., 2007. / Title from title page display. Includes bibliographical references.
20

Analyse der Genregulation in Medicago truncatula und Pisum sativum während der Entwicklung arbuskulärer Mykorrhiza

Grunwald, Ulf. January 2004 (has links) (PDF)
Marburg, Universiẗat, Diss., 2004.

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