The study characterized the morphological differences among Cisco between geographic arms and depth strata, and determined the trophic positioning of various morphs, based on twelve years of capture data and specimens using morphological measurements, meristic counts, stomach contents, stable isotopes and fatty acids. Two main Cisco morpho-types were identified, a shallow water form and a deep water form that mainly differed in traits associated with swimming and feeding. These results were reinforced by the results on trophic ecology of morphs that demonstrated that each morph occupied a distinct niche. Results showed that shallow water groups had a more varied diet with a high percentage of copepods being ingested whereas deep-water groups showed preferences towards mysids. Although a consistent pattern in differences among shallow and deep morphs was observed, there was some degree of variation among arms of the lake suggesting a degree of isolation and adaptation to local conditions in each area of the lake. For example, in Smith Arm, morphological and dietary differences were found such as narrower body depth and a dietary preference towards daphnia. / February 2017