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Určení vztahu spiroergometrického vyšetření při jízdě na rychlostní kanoi a při pádlování na trenažéru u elitních českých kanoistů / Determining the relationship of spiroergometric testing on the canoe ergometer and in canoe sprint by elite competitorsMiškovský, Radek January 2018 (has links)
Title: Determining the relationship of spiroergometric testing on the canoe ergometer and in canoe sprint by elite competitors. Objectives: The aim of the study was to determine the relationship between the results of aerobic exercise diagnostics in canoeing and on the canoe ergometer by Czech national team members in canoe sprint. Methods: Within our study we tried to find out and compare the physiological response of two functional load exercise diagnostics in canoeing and on the canoe ergometer. To obtain the functional values of physiological indicators in both tests we used mobile spiroergometric equipment Cortex Metamax 3B and sport tester Polar RS 800. Results: Testing canoeists paddling on flat water reached the following average functional values: maximal heart rate (HR) 181 min-1 (±3,9), maximal values of blood lactate (LA) 8,04 ml.l-1 (±1,6), maximal oxygen consumption (VO2max) 51,6 ml.kg.min-1 (±6,7), maximal ventilation (VEmax) 140,2 l.min-1 (±26,5), respiratory rate (RR) 51 min-1 (±3,7), metabolic load (Rmax) 1,14 (±0,07) and anaerobic threshold (ANT) 166 (±7,2). When paddling the canoe ergometer, they reached the average maximum values: maximal heart rate (HR) 181 min-1 (±5,2), maximal values of blood lactate (LA) 9,1 mmol.l-1 (±2,9), maximal oxygen consumption (VO2max) 56,4...
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Porovnání vnitřního zatížení u střídavého běhu dvoudobého a soupažného běhu prostého při sprintu na kolečkových lyžích / Comparison of load during diagonal striding and double poling on the roller ski during sprintČechová, Anna January 2020 (has links)
Title: Comparison of internal loading on the organism by double poling and diagonal striding during the sprint on the roller ski Objectives: The aim of the diploma thesis is to compare the internal loading using the Cortex Metamax 3B and the SenzoStar GL devices in a two-hundred meters long section performed by the maximum effort between the double poling and diagonal striding about the 10.4% climb on roller skis. Methods: The diploma thesis is conceived as an empirical field research, the aim of which is to compare a double poling and diagonal striding in terms of internal loading on roller skis during sprint. The test subject will be a group of cross-country skiers and long-distance skiers. Results: Using the measured values of the internal loading and the measured time, we found differences during comparing the diagonal striding and double polling in specific values of SF, VO2 / kg, VE. the conclusion was that diagonal striding is faster than double poling to the uphill on roller skis. Keywords: XC-skiing, roller skis, Cortex MetaMax 3B, oxygen consumption, heart rate, lactate
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Vision Beyond Optics: Standardization, Evaluation and Innovation for Fluorescence Microscopy in Life SciencesHuisman, Maximiliaan 01 April 2019 (has links)
Fluorescence microscopy is an essential tool in biomedical sciences that allows specific molecules to be visualized in the complex and crowded environment of cells. The continuous introduction of new imaging techniques makes microscopes more powerful and versatile, but there is more than meets the eye. In addition to develop- ing new methods, we can work towards getting the most out of existing data and technologies. By harnessing unused potential, this work aims to increase the richness, reliability, and power of fluorescence microscopy data in three key ways: through standardization, evaluation and innovation.
A universal standard makes it easier to assess, compare and analyze imaging data – from the level of a single laboratory to the broader life sciences community. We propose a data-standard for fluorescence microscopy that can increase the confidence in experimental results, facilitate the exchange of data, and maximize compatibility with current and future data analysis techniques.
Cutting-edge imaging technologies often rely on sophisticated hardware and multi-layered algorithms for reconstruction and analysis. Consequently, the trustworthiness of new methods can be difficult to assess. To evaluate the reliability and limitations of complex methods, quantitative analyses – such as the one present here for the 3D SPEED method – are paramount.
The limited resolution of optical microscopes prevents direct observation of macro- molecules like DNA and RNA. We present a multi-color, achromatic, cryogenic fluorescence microscope that has the potential to produce multi-color images with sub-nanometer precision. This innovation would move fluorescence imaging beyond the limitations of optics and into the world of molecular resolution.
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