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Desproteinização do esmalte associada à técnica de remineralização no clareamento em consultório / Enamel deproteinization associated to remineralization technique on in-office bleachingGomes, Mauricio Neves 27 September 2011 (has links)
Objetivo: avaliar cor, brilho, rugosidade e alterações ultraestruturais do esmalte dental clareado com peróxido de hidrogênio a 35 %, submetido ao tratamento prévio com agente desproteinizante, ou ao tratamento posterior com o agente remineralizador fosforopeptídeo de caseína/fosfato de cálcio amorfo (ACP-CPP). Material e Métodos: Os grupos experimentais foram: GC (controle/consultório): H2O2 a 35 % - 4 sessões de 8 min; GE1 (primer+consultório): NaOCl 5,25 % por 1 min, aplicação do H2O2 a 35 % como no GC; e GE2 (consultório+ACP): GC + ACP-CPP diariamente por 7 dias. Fragmentos contendo esmalte e dentina (n=8), obtidos de dentes bovinos, foram utilizados para avaliar cor, brilho e a rugosidade. Alteração de cor (E), parâmetros L* e b* foram determinados com colorímetro e o brilho superficial com glossímetro antes, imediatamente após (1h), 4 e 7 dias após o tratamento. Parâmetros de rugosidades, Ra, Rt e RSm, foram obtidos com perfilômetro de contato antes, imediatamente após o tratamento e 7 dias após os tratamentos. Os resultados de E, brilho superficial e rugosidade foram avaliados separadamente usando ANOVA 2 fatores e teste de Tukey (p=0,05). Para avaliar a alteração ultraestrutural, dentes pré-molares humanos, seccionados nos sentidos vestibulo-lingual e mesio distal foram observados em microscópio eletrônico de varredura, por emissão de campo, e realizada a quantificação de elementos químicos por EDS. Análise tridimensional da estrutura do esmalte foi realizada por microtomografia computadorizada (micro-CT) com resolução 11,24 m (n=8). Foram realizadas análises dos parâmetros estruturais: espessura estrutural (St.Th.), separação estrutural (St.Sp.) e índice de fragmentação (Fr.I.) antes e após os tratamentos em duas regiões: ROI 1= 56,2 m e ROI 2= 110,2 m, ambas a partir da superfície vestibular. Foi utilizado o teste t pareado para análise estatística de cada parâmetro estrutural. Resultados: Não houve diferença estatística entre os diferentes tratamentos de superfície para E, Ra e RSm. Imediatamente após o clareamento (1h) ocorreu maior aumento do L* e queda do brilho superficial que se manteve por 7 dias. O uso de agente desproteinizante em dentes bovinos não acentuou a redução do brilho do esmalte, mas a aplicação de ACP-CPP acarretou em maior perda brilho e aumento nos valores de rugosidade após 7 dias para Rt. A aplicação do agente desproteinizante previamente ao clareamento em dente humano revelou uma superfície mais lisa, sem alterar os parâmetros estruturais. Há uma maior quantidade de cálcio, formação de um manto de recobrimento após aplicação de ACP-CPP em torno dos prismas de esmalte, aumento de St.Th de 4,1m, menor espaçamento entre os cristais de hidroxiapatita e redução de St.Sp em 0,8 m e de Fr.I em 0,01 no ROI-1 após 7 dias. Conclusão: O uso de agente desproteinizante não altera a cor, brilho e a ultraestrutura inorgânica. A aplicação de ACP-CPP após a técnica de clareamento de consultório não contribui para alteração de cor, mas reduz o brilho e altera a ultraestrutura da porção mais externa do esmalte após 7 dias / Purpose: To evaluate color, gloss, roughness and ultrastructural changes of enamel bleached with 35% hydrogen peroxide, subjected to previous treatment with deproteinized agent, or later treatment with remineralizing agent casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Materials and Methods: The experimental groups were: GC (control + in-office): 35% H2O2 - 4 sessions of 8 min; GE1(primer+in-office): 5.25% NaOCl during 1 min before the application of 35% H2O2 as done in GC, and GE2 (in-office+ACP-CPP): GC + ACP-CPP, daily applied during 7 days. Enamel and dentin blocks (n=8), obtained from bovine tooth, were used to evaluate color, gloss and roughness. Color changes (E), L* and b* parameters were done with a colorimeter and surface gloss with a glossimeter, before, immediately after (1h), 4 and 7 days after treatment. Roughness parameters, Ra, RT and Rsm, were done with a contact perfilometer before, immediately after and 7 days after treatments. ANOVA two-way and Tukeys test were performed to evaluate E, gloss and roughness separately (p=0.05). To access human pre-molar ultrastructural changes, teeth were cross-sectioned buccal-lingual and disto-mesio observed by scanning electron microscope, field emission gun, EDS to quantify chemical elements. Enamel three-dimensional images were analysed with microcomputed tomography (micro-CT) with resolution 11,24m (n=8). Structural parameters were analyzed: structural thickness (St.Th.), structural separation (St.Sp.) and fragmentation index (Fr.I.) before and after treatments in two regions of interest:ROI 1= 56,2m and ROI 2= 110,2 m, both from buccal surface. Paired t-test was done for analyses of each structural parameter. Results: There was no statistical difference among surface treatments to E, Ra and Rsm. Immediately after bleaching (1h) occured highest L* increase and decrease of surface gloss which remained until 7 days. Deproteinized agent applied on bovine tooth not emphasized enamel gloss reduction, but the CPP-ACP has resulted in a higher gloss reduction and roughness increase (Rt parameter) after 7 days. Deproteinized agent application previous to in- office bleaching observed a smooth surface, without structural parameters changes. There is a greater calcium quantity, forming a cover mantle after CPP-ACP application around enamel prisms, St.Th increase of 4,1m, less spacing between hydroxyapatite crystals and reductions of St.Sp of 0,8 m and Fr.I of 0,01 on ROI-1 after 7 days. Conclusion: Application of deproteinized agent does not change bovine enamel color, gloss and human enamel inorganic ultrastructure. CPP-ACP application after in-office bleaching does not contribute to color change, but decrease gloss of bovine enamel and change human enamel outermost ultrastructure portion after 7 days.
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Desproteinização do esmalte associada à técnica de remineralização no clareamento em consultório / Enamel deproteinization associated to remineralization technique on in-office bleachingMauricio Neves Gomes 27 September 2011 (has links)
Objetivo: avaliar cor, brilho, rugosidade e alterações ultraestruturais do esmalte dental clareado com peróxido de hidrogênio a 35 %, submetido ao tratamento prévio com agente desproteinizante, ou ao tratamento posterior com o agente remineralizador fosforopeptídeo de caseína/fosfato de cálcio amorfo (ACP-CPP). Material e Métodos: Os grupos experimentais foram: GC (controle/consultório): H2O2 a 35 % - 4 sessões de 8 min; GE1 (primer+consultório): NaOCl 5,25 % por 1 min, aplicação do H2O2 a 35 % como no GC; e GE2 (consultório+ACP): GC + ACP-CPP diariamente por 7 dias. Fragmentos contendo esmalte e dentina (n=8), obtidos de dentes bovinos, foram utilizados para avaliar cor, brilho e a rugosidade. Alteração de cor (E), parâmetros L* e b* foram determinados com colorímetro e o brilho superficial com glossímetro antes, imediatamente após (1h), 4 e 7 dias após o tratamento. Parâmetros de rugosidades, Ra, Rt e RSm, foram obtidos com perfilômetro de contato antes, imediatamente após o tratamento e 7 dias após os tratamentos. Os resultados de E, brilho superficial e rugosidade foram avaliados separadamente usando ANOVA 2 fatores e teste de Tukey (p=0,05). Para avaliar a alteração ultraestrutural, dentes pré-molares humanos, seccionados nos sentidos vestibulo-lingual e mesio distal foram observados em microscópio eletrônico de varredura, por emissão de campo, e realizada a quantificação de elementos químicos por EDS. Análise tridimensional da estrutura do esmalte foi realizada por microtomografia computadorizada (micro-CT) com resolução 11,24 m (n=8). Foram realizadas análises dos parâmetros estruturais: espessura estrutural (St.Th.), separação estrutural (St.Sp.) e índice de fragmentação (Fr.I.) antes e após os tratamentos em duas regiões: ROI 1= 56,2 m e ROI 2= 110,2 m, ambas a partir da superfície vestibular. Foi utilizado o teste t pareado para análise estatística de cada parâmetro estrutural. Resultados: Não houve diferença estatística entre os diferentes tratamentos de superfície para E, Ra e RSm. Imediatamente após o clareamento (1h) ocorreu maior aumento do L* e queda do brilho superficial que se manteve por 7 dias. O uso de agente desproteinizante em dentes bovinos não acentuou a redução do brilho do esmalte, mas a aplicação de ACP-CPP acarretou em maior perda brilho e aumento nos valores de rugosidade após 7 dias para Rt. A aplicação do agente desproteinizante previamente ao clareamento em dente humano revelou uma superfície mais lisa, sem alterar os parâmetros estruturais. Há uma maior quantidade de cálcio, formação de um manto de recobrimento após aplicação de ACP-CPP em torno dos prismas de esmalte, aumento de St.Th de 4,1m, menor espaçamento entre os cristais de hidroxiapatita e redução de St.Sp em 0,8 m e de Fr.I em 0,01 no ROI-1 após 7 dias. Conclusão: O uso de agente desproteinizante não altera a cor, brilho e a ultraestrutura inorgânica. A aplicação de ACP-CPP após a técnica de clareamento de consultório não contribui para alteração de cor, mas reduz o brilho e altera a ultraestrutura da porção mais externa do esmalte após 7 dias / Purpose: To evaluate color, gloss, roughness and ultrastructural changes of enamel bleached with 35% hydrogen peroxide, subjected to previous treatment with deproteinized agent, or later treatment with remineralizing agent casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Materials and Methods: The experimental groups were: GC (control + in-office): 35% H2O2 - 4 sessions of 8 min; GE1(primer+in-office): 5.25% NaOCl during 1 min before the application of 35% H2O2 as done in GC, and GE2 (in-office+ACP-CPP): GC + ACP-CPP, daily applied during 7 days. Enamel and dentin blocks (n=8), obtained from bovine tooth, were used to evaluate color, gloss and roughness. Color changes (E), L* and b* parameters were done with a colorimeter and surface gloss with a glossimeter, before, immediately after (1h), 4 and 7 days after treatment. Roughness parameters, Ra, RT and Rsm, were done with a contact perfilometer before, immediately after and 7 days after treatments. ANOVA two-way and Tukeys test were performed to evaluate E, gloss and roughness separately (p=0.05). To access human pre-molar ultrastructural changes, teeth were cross-sectioned buccal-lingual and disto-mesio observed by scanning electron microscope, field emission gun, EDS to quantify chemical elements. Enamel three-dimensional images were analysed with microcomputed tomography (micro-CT) with resolution 11,24m (n=8). Structural parameters were analyzed: structural thickness (St.Th.), structural separation (St.Sp.) and fragmentation index (Fr.I.) before and after treatments in two regions of interest:ROI 1= 56,2m and ROI 2= 110,2 m, both from buccal surface. Paired t-test was done for analyses of each structural parameter. Results: There was no statistical difference among surface treatments to E, Ra and Rsm. Immediately after bleaching (1h) occured highest L* increase and decrease of surface gloss which remained until 7 days. Deproteinized agent applied on bovine tooth not emphasized enamel gloss reduction, but the CPP-ACP has resulted in a higher gloss reduction and roughness increase (Rt parameter) after 7 days. Deproteinized agent application previous to in- office bleaching observed a smooth surface, without structural parameters changes. There is a greater calcium quantity, forming a cover mantle after CPP-ACP application around enamel prisms, St.Th increase of 4,1m, less spacing between hydroxyapatite crystals and reductions of St.Sp of 0,8 m and Fr.I of 0,01 on ROI-1 after 7 days. Conclusion: Application of deproteinized agent does not change bovine enamel color, gloss and human enamel inorganic ultrastructure. CPP-ACP application after in-office bleaching does not contribute to color change, but decrease gloss of bovine enamel and change human enamel outermost ultrastructure portion after 7 days.
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Diagnostic accuracy and reproducibility of conventional and novel caries detection methods as determined by histology and micro-CT : a study in vitroAl Jamaan, Tamer Saleh January 2016 (has links)
No description available.
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Ichnology, sedimentology, stratigraphy, and trace fossil-permeability relationships in the Upper Cretaceous Medicine Hat Member, Medicine Hat gas field, southeast Alberta, CanadaLa Croix, Andrew David Unknown Date
No description available.
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Inferring mode of locomotion through microscopic cortical bone analysis: a comparison of the third digits of Homo sapiens and Ursus americanus using Micro-CTHarrison, Kimberly D. 18 December 2012 (has links)
Bone is a 3D dynamic and unique tissue that structurally adapts in response to mechanical stimuli. Comparative skeletal morphology is commonly utilized to infer ancient hominins' modes of locomotion; however, instances of remarkable gross similarity despite different modes of locomotion do occur. A common cited example is the similarity between the skeletal elements of bipedal human (Homo sapiens) hands/feet and quadrupedal black bear (Ursus americanus) front/hind paws. Through novel 3D Micro-CT and 2D histomorphology analysis, this thesis tests the hypothesis that a 3D microscopic analysis of biomechanically regulated cortical bone structures provides a more representative and accurate means to infer a species' mode of locomotion. Micro-CT data were collected at the mid-diaphysis of human (n=5) and bear (n=5) third metacarpal/metatarsal pairs and compared with independent and paired t-tests, Pearson correlation coefficients and Bland-Altman plots. Bone microarchitecture is quantifiable in 3D and accessible through non-destructive Micro-CT. Interspecies variation was present, however no significant cortical differences between elements of humans and bears was found. Histological inspection revealed further variation between and within species and element. A key limitation was sample size and further investigation of the relationship between mechanical loading and mode of locomotion is warranted.
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Spectral Micro-CT Imaging of Ex Vivo Atherosclerotic PlaqueZainon, Rafidah Binti January 2012 (has links)
The goal of this research was to demonstrate the potential of spectral CT for the discrimination of vulnerable atherosclerotic plaques. It was proposed that spectral CT has the potential to identify the presence of specific markers for vulnerable plaques: iron deposits and lipid core. A spectral micro-CT system incorporating the latest Medipix spectroscopic photon- counting detectors was commissioned for this purpose. Using spectroscopic methods developed with this system, it was possible to distinguish the presence of iron deposits and lipid core within ex vivo atherosclerotic plaques. Atherosclerosis or hardening of arteries is a systemic disease of the vessel wall that occurs in the aorta, carotid, coronary and peripheral arteries. It is characterised by the deposition of calcified plaques on the innermost layer of the artery wall. Vulnerable plaques are unstable, prone to rupture and put the person at risk of cardiovascular events and strokes. Factors that may lead to plaque instability are lipid content and iron deposits. This preclinical study is a precursor to the development of a clinical technique that will enable vulnerable atherosclerotic plaques to be identified in vivo prior to treatment or removal. Following a preliminary study on atherosclerotic plaques with a prototype system, the MARS-CT3 spectral micro-CT system incorporating Medipix3 was developed and commissioned for further plaque studies. The spectral CT data sets acquired by this system were assessed visually for morphology and analysed for material composition using a linear algebra method. The results were correlated with photography and histology (the histology is the current gold standard).
The presence of iron and lipid can be differentiated from the background soft-tissue using a linear algebra method. However the quantification of iron in the presence of calcium is not currently possible without additional data or constraints. Nevertheless the presence of iron deposits within the plaques can be distinguished in the high resolution MARS-CT images and has been correlated with photographic and histological evidence. Thus, using the high spatial resolution spectral data from MARS-CT, the discrimination of lipid core and iron deposits within ex vivo advanced human atherosclerotic plaques is feasible. This may provide the basis for the development of a clinical technique that will identify vulnerable plaques in vivo by high resolution spectral CT.
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Inferring mode of locomotion through microscopic cortical bone analysis: a comparison of the third digits of Homo sapiens and Ursus americanus using Micro-CTHarrison, Kimberly D. 18 December 2012 (has links)
Bone is a 3D dynamic and unique tissue that structurally adapts in response to mechanical stimuli. Comparative skeletal morphology is commonly utilized to infer ancient hominins' modes of locomotion; however, instances of remarkable gross similarity despite different modes of locomotion do occur. A common cited example is the similarity between the skeletal elements of bipedal human (Homo sapiens) hands/feet and quadrupedal black bear (Ursus americanus) front/hind paws. Through novel 3D Micro-CT and 2D histomorphology analysis, this thesis tests the hypothesis that a 3D microscopic analysis of biomechanically regulated cortical bone structures provides a more representative and accurate means to infer a species' mode of locomotion. Micro-CT data were collected at the mid-diaphysis of human (n=5) and bear (n=5) third metacarpal/metatarsal pairs and compared with independent and paired t-tests, Pearson correlation coefficients and Bland-Altman plots. Bone microarchitecture is quantifiable in 3D and accessible through non-destructive Micro-CT. Interspecies variation was present, however no significant cortical differences between elements of humans and bears was found. Histological inspection revealed further variation between and within species and element. A key limitation was sample size and further investigation of the relationship between mechanical loading and mode of locomotion is warranted.
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Ichnology, sedimentology, stratigraphy, and trace fossil-permeability relationships in the Upper Cretaceous Medicine Hat Member, Medicine Hat gas field, southeast Alberta, CanadaLa Croix, Andrew David 11 1900 (has links)
The Upper Cretaceous Medicine Hat Member (Niobrara Formation) in western Canada contains abundant reserves of biogenic natural gas. In the Medicine Hat gas field area of southeast Alberta, nineteen cored intervals were examined and classified based on primary physical and biogenic sedimentary structures. Core analysis and stratigraphic mapping determined that the Medicine Hat Member strata consist of stacked, regionally extensive, lobate geobodies that prograde to the north. Employing spot-minipermeametry, the effect of biogenic rock fabrics on the reservoir characteristics was assessed. X-ray micro-computed tomography was conducted on four samples from a reservoir interval to visualize the geometry and distribution of burrow-associated heterogeneity. The results demonstrate that planiform bioturbate textures locally enhance the storage and transmission of natural gas in Medicine Hat reservoirs.
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An in-vitro comparative micro-computed tomographic evaluation of three obturation systemsKabini, S.N. January 2017 (has links)
Magister Chirurgiae Dentium - MChD (Prosthodontics) / Gaps or voids between walls of root canal and obturation material may lead to re-infection of
the obturated root canal. Therefore, adaptation of the obturation material to dentine walls is
essential for the success of root canal treatment.
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Craniofacial Bone Density is Regulated by Thyroid Hormone in Zebrafish:May, Catherine M. January 2019 (has links)
Thesis advisor: Sarah McMenamin / Thyroid hormone (TH) facilitates developmental transitions, particularly by modulating cell proliferation and differentiation. Its role in regulating skeletal growth is well documented. Previous work from our lab and others have demonstrated that hypo- and hyperthyroid fish display changes in bone shape, ossification and the timing of ossification5. Zebrafish (Danio rerio) develop bone quickly, grow indefinitely throughout their lives, are highly amenable for imaging, and are a valuable model for skeletal biology research. Using Danio rerio, we sought to study the long-term effects of TH on bone density by rearing and comparing normal euthyroid (Eu) with a transgenically thyroid-ablated hypothyroid (TH-) and mutant hyperthyroid (TH+) fish. We found that TH strongly affects bone density and volume. We further hypothesize that TH is critical for the timing and fidelity of skeletogenesis. In hormone-dysregulated fish, we found that massive bone and cartilage exostoses grow on the dentary. Thyroid hormone’s effects are highly bone-specific: in TH- fish, we see reduced density in many craniofacial bones, but also increased volume and mineralization in other regions of the dentary. These data suggest that TH plays a critical role in coordinating bone mineralization with growth. / Thesis (MS) — Boston College, 2019. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Biology.
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