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A Comparison of Microbial Contamination in Commercial Intravenous Fluids Packaged in Glass and Flexible Plastic ContainersGuynn, James Bruce 01 January 1973 (has links)
An original study was undertaken in the pharmacy and at the nursing unit simulating conditions of actual use to determine contamination rates for intravenous fluids in glass bottles and flexible plastic bags.
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Microbial contamination of enteral feedsLee, Cheuk-hung, 李卓雄 January 2004 (has links)
published_or_final_version / Ecology and Biodiversity / Doctoral / Doctor of Philosophy
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The effects of varying extrinsic parameters and specific pretreatments in whole fish and prepared fish filletsLambropoulou, Kyriaki A. January 1999 (has links)
No description available.
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Microbial and physico-chemical quality of some surface water resources in Durban, South Africa.Naicker, Kovashnee. January 2010 (has links)
Microbial and chemical contamination of inland and coastal waters in Southern Africa is a major
challenge facing the water industry and regulatory authorities. Increased stresses on these surface water
resources through human and environmental influences have resulted in deteriorating water quality that
has severely encumbered the country’s capability to provide sufficient water to meet its needs and to
ensure environmental sustainability. In addition, indiscriminate use of antibiotics has resulted in
widespread contamination of surface waters, leading to accelerated development of antibiotic resistance
and proliferation of resistant water-borne diarrhoeal-related pathogens, such as Escherichia coli and
Vibrio cholerae. Despite the high level of contamination of South African surface waters, the
microbiological quality of rivers and beaches in Durban, South Africa, have not been adequately
investigated. Therefore, the current study assessed the seasonal fluctuations of the microbial and physicochemical
quality of two rivers (Umgeni River and Umdloti River) and six beaches (Virginia Aerodome,
Beachwood, Umgeni South, Battery, Sunkist, Addington) in Durban, using several bacterial indicator
organisms and physico-chemical parameters as indices. The antibiotic resistance profiles (ARPs) of E.
coli and V. cholerae strains, recovered from the water samples, were determined and changes in the
microbial community of the water samples were monitored over a seasonal cycle, using denaturing
gradient gel electrophoresis (DGGE). Spatial and seasonal fluctuations of the physico-chemical
parameters differed significantly (p < 0.05) among the water samples with high heavy metal
concentrations detected across the seasonal cycle. Temperature profiles ranged from 13°C to 26.5°C for
the Umgeni River, 13°C to 27°C for the beaches and 12°C to 26°C for the Umdloti River while pH
ranged from 6.30 to 8.45 (Umgeni River), 6.37 to 8.30 (beaches) and 5.96 to 7.94 (Umdloti River).
Turbidity ranged from 0.53 NTU to 15.6 NTU (Umgeni River); 0.57 NTU to 2.37 NTU (beaches) and
2.23 NTU to 18.8 NTU (Umdloti River). During spring and summer, all river and beach water samples
had < 500 μg/L phosphate concentrations; however, these concentrations increased significantly (p <
0.05) during autumn and winter in both rivers. Majority of the samples had low concentrations of
ammonia and nitrates. Sulphate concentrations for the beach samples ranged from 2355 mg/L (B5 –
summer) to 2899 mg/L (B2 – winter) as compared to the Umgeni and Umdloti Rivers which ranged from
3.90 mg/L (A4 – autumn) to 2762 mg/L (A1 – summer) and 4.47 mg/L (C4 – winter) to 168 mg/L (C1 –
winter), respectively. According to the South African Target Quality Range guidelines for the heavy
metals (in surface waters), all river and beach water samples exceeded the set limits for lead (Pb2+),
mercury (Hg2+) and cadmium (Cd2+) across all seasons. During spring and summer all water samples
complied with the aluminium guideline of 0 – 0.15 mg/L. Bacterial population profiles indicated that all
sampling points failed to comply with the set guidelines (domestic use) for presumptive total coliform
(TC), faecal coliform (FC) and total heterotrophic bacterial (THB) counts during all four seasons.
Estimated TC, FC and THB populations as high as 8.6 x 101, 3.7 x 101 and 2.15 x 105 cfu/100ml,
respectively, were obtained for some of the samples with peak indicator levels and generally a higher
microbial load observed during the summer season. High prevalence of resistance to ampicillin [67.82%
(Umgeni River)] was encountered among the E. coli isolates from the water samples followed by
amikacin [53.33% (Umdloti River)], augmentin [49.6% (Umdloti River)], tetracycline [42% (Umgeni
River)], streptomycin [37.1% (beaches)] and cotrimoxazole [33% (Umgeni River)]. The most frequently
encountered form of resistance among the V. cholerae isolates was against cotrimoxazole [93.34%
(Umgeni River)], streptomycin [84% (beaches)], erythromycin [78.7% (Umgeni River)], trimethoprim
[77.7% (Umdloti River)], rifampicin [70% (Umgeni River)] and cefoxitin [45% (Umdloti River)]. Multidrug
resistance among the E. coli isolates was indicated by twenty nine (Umgeni River), twenty six
(beaches) and fourteen (Umdloti River) different resistance patterns, while the V. cholerae isolates
produced eighteen (Umgeni River), thirty five (beaches) and twenty nine (Umdloti River) different
resistance patterns. In addition, proportional resistances of the E. coli and V. cholerae strains to the
different classes of antibiotics ranged from six to eleven and four to eleven different antibiotic classes,
respectively. The present study suggests that the bacterial communities detected in the water samples
collected from the rivers and beaches in Durban, followed seasonal dynamics and could possibly be the
consequence of fluctuations in certain environmental factors. A total of 87 different DGGE bands were
detected among the Umgeni River water samples, 127 different DGGE bands among the six beach water
samples and 107 bands in the Umdloti River samples, over the four seasons. Twenty one dominant bands
were found among all sampling sites, indicating widespread phylotypes, whereas 14 bands were
exclusively detected at only one sampling site (C1) potentially indicating unique phylotypes. Some bands
appeared all year-round, whereas some other bands were specific to a particular season. Overall, the
present study successfully demonstrated the poor microbiological quality of the investigated river and
beach water resources which raise concerns over the management of these water resources and the
subsequent deleterious effects these waters could have on the end users. This emphasizes the need for
implementation of improved management strategies of these river catchments and beaches for continued
sustainability. Furthermore, the high level of multi-antibiotic resistance demonstrated by the E. coli and V.
cholerae strains, recovered from the water samples, reiterates the need to continuously monitor the
changing trends in antimicrobial resistance patterns of these diarrhoeal-related bacterial pathogens.
Therefore, continued surveillance of these surface waters used for recreational or domestic purposes and
development of adequate prevention strategies are needed for public health reasons. Lastly, combining the
use of conventional faecal indicators with molecular-based techniques, such as DGGE, can provide more
information on the microbial load and diversity of surface waters. In addition, information regarding the
effects of seasonal variations on microbial diversity as observed in this study is important for the
sustainable management of surface water resources. / Thesis (M.Sc.)-University of KwaZulu-Natal, 2010.
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The use of microbial community fingerprinting as a marker for tracking the source of water application to pathogen and groundwater source tracking /Clement, Mary January 2010 (has links)
Thesis (M.S.)--West Virginia University, 2010. / Title from document title page. Document formatted into pages; contains ix, 49 p. : ill. (some col.), col. maps. Includes abstract. Includes bibliographical references (p. 47-49).
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Quantitative analyses of F+ specific RNA coliphages /Kirs, Marek. January 2005 (has links)
Thesis (Ph. D.)--University of Rhode Island, 2005. / Typescript. Includes bibliographical references (leaves 129-143).
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Health and safety aspects of the use of products from urine-diversion toiletsPhasha, Mmolawa Cynthia. January 2005 (has links)
Thesis (M. Sc.)(Microbiology)--University of Pretoria, 2005. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.
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Rhizosphere microbial diversity in PAH's contaminated and uncontaminated soilRandima, Livhuwani Priscilla. January 2009 (has links)
Thesis (M.Sc.)(Microbiology))--University of Pretoria, 2009. / Summary in English. Includes bibliographical references.
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Investigation of environmental microorganisms associated with the intrinsic microbial contamination of an alcohol-free mouthwashTrickett, Michelle D. January 2007 (has links)
Theses (M.S.)--Marshall University, 2007. / Title from document title page. Includes abstract. Document formatted into pages: contains viii, 37 pages. Bibliography: p. 36-37.
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Control of microbial proliferation on sorghum during maltingLefyedi, Mathoto Lydia. January 2007 (has links)
Thesis (Ph.D.)(Food Science)--University of Pretoria, 2006. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.
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