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To prevent melting of turbine blades, numerous cooling schemes have been developed to cool the blades using cooler air from the compressor. Unfortunately, the clearance gap between adjacent hub sections allows coolant to leak into the hub region. Coolant flow also leaks into the hub region through gaps between individual stages. The results of a combined experimental and computational study of cooling along the hub of a first stage turbine blade caused by leakage flows are discussed in detail. Additionally, this study examines a novel cooling feature, called a microcircuit, which combines internal convective cooling with external film cooling. For the experimental investigation, scaled up blades were tested in a low speed wind tunnel. Adiabatic effectiveness measurements were made with infrared thermography of the entire hub region for a range of leakage flow conditions. For the computations, a commercially available computational fluid dynamics (CFD) code, FLUENT 6.0, was used to simulate the various flows. Results show that featherseal leakage flows provide small cooling benefits to the hub. Increases in featherseal flow provide no additional cooling to the hub region. Unlike the featherseal, leakage flows from the front rim provide ample cooling to the hub region, especially the leading edge of the blade passage. None of the leakage flows provide significant cooling to the pressure side region of the hub or trailing edge suction side. With the addition of the hub microcircuits, there is improved hub cooling of the suction side of the blades. Though the coolant exit uniformity was low and affected by the featherseal flow, the microcircuits were shown to provide more cooling along the hub region. Good agreements were observed between the computational and experimental results, though computations over-predicted front rim cooling and microcircuit uniformity. / Master of Science

Efficiency and power output of gas turbines improve with an increase in turbine inlet temperatures, and blade designers continually seek out new methods of increasing these temperatures. Increases in turbine inlet temperatures are achieved by utilizing a combination of internal convective cooling and external film-cooling. This study will evaluate several novel cooling schemes for turbine airfoils, called microcircuits. Microcircuits are placed inside the turbine blade wall, and the features turbulate the air and increase heat transfer surface area, thereby augmenting convective cooling. The coolant flow then exits internal cooling passages to the external side of the blade. Here the coolant forms a protective layer along the external surface of the blade to protect the blade from the heated mainstream flow. In the current study, a low-speed large-scale wind tunnel facility was developed to measure internal heat transfer coefficients and external adiabatic effectiveness, using thermal liquid crystallography and infrared thermography. This test facility is unique in that it can be used to test the effects of internal cooling features on external film cooling. Results show that the highest augmentations in internal heat transfer were seen at the lowest Reynolds numbers. Internal features affected the shapes of external film-cooling contours, but the magnitudes of the spanwise averaged values did not change significantly with changes in internal geometry. / Master of Science

In a gas turbine engine, turbine blades are exposed to temperatures above their melting point. Film-cooling and internal cooling techniques can prolong blade life and allow for higher engine temperatures. This study examines a novel cooling technique called a microcircuit, which combines internal convection and pressure side injection on a turbine blade tip. Holes on the tip called dirt purge holes expel dirt from the blade, so other holes are not clogged. Wind tunnel tests are used to observe how effectively dirt purge and microcircuit designs cool the tip. Tip gap size and blowing ratio are varied for different tip cooling configurations. Results show that the dirt purge holes provide significant film cooling on the leading edge with a small tip gap. Coolant injected from these holes impacts the shroud and floods the tip gap reducing tip leakage flow. With the addition of a microcircuit, coolant is delivered to a larger area of the tip. In all cases, cooling levels are higher for a small tip gap than a large tip gap. Increased blowing ratio does not have a dramatic effect on microcircuit film-cooling at the midchord but does improve internal cooling from the microcircuit. While the combined dirt purge holes and microcircuit cool the leading edge and midchord areas, there remains a small portion of the trailing edge that is not cooled. Also, results suggest that blowing from the microcircuit diminishes the tip leakage vortex. Overall, the microcircuit appears to be a feasible method for prolonging blade life. / Master of Science

Turbine airfoils are exposed to the hottest temperatures in the gas turbine with temperatures typically exceeding the melting point of the blade material. Cooling methods investigated in this computational study included parasitic cooling flow losses, which are inherent to engines, and microcircuit channels. Parasitic losses included dirt purge holes, located along the blade tip, and platform leakage flow, which result from gaps between various turbine components. Microcircuits are a novel cooling technique involving small air passages placed near the airfoil surface to enhance internal cooling. This study evaluated the benefit of external film-cooling flow exhausted from strategically placed microcircuits. Along the blade tip, predictions showed mid-chord cooling was independent of the blowing from microcircuit exits. The formation of a pressure side vortex was found to develop for most microcircuit film-cooling cases. Significant leading edge cooling was obtained from coolant exiting from dirt purge holes with a small tip gap while little cooling was seen with a large tip gap. Along the blade platform, the migration of coolant from the front leakage was shown to cool a considerable part of the platform. Several hot spots were predicted along the platform, which were circumvented through the placement of microcircuit channels. Ingestion of hot mainstream gas was predicted along the aft portion of the gutter and agreed with distress exhibited by actual gas turbine engines. / Master of Science

Aircraft engine manufacturers strive for greater performance and efficiency by continually increasing the turbine inlet temperature. High turbine inlet temperatures significantly degrade the lifetime of components in the turbine. Modern gas turbines operate with turbine inlet temperatures well above the melting temperature of key turbine components. Without active cooling schemes, modern turbines would fail catastrophically. This study will evaluate a novel cooling scheme for turbine airfoils, called microcircuit cooling, in which small cooling channels are located extremely close to the surface of a turbine airfoil. Coolant bled from the compressor passes through the microcircuits and exits through film cooling slots. On further cooling benefit is that the microcircuit passages are filled with irregular pin fin features that serve to increase convective cooling through the channels. Results from this study indicate a strong interaction between the internal microcircuit features and the external film-cooling from the slot exit. Asymmetric cooling patterns downstream of the slot resulted from the asymmetric pin fin design within the microcircuit. Adiabatic effectiveness levels were found to be optimum for the slot design at a blowing ratio of 0.37. The pin fin arrangement along with the impingement cooling at the microcircuit entrance increased the area-averaged heat transfer by a factor of three, relative to an obstructed channel, over a Reynolds range of 5,000 to 15,000. / Master of Science

Contexte La connectomique, ou la cartographie des connexions neuronales, est un champ de recherche des neurosciences évoluant rapidement, promettant des avancées majeures en ce qui concerne la compréhension du fonctionnement cérébral. La formation de circuits neuronaux en réponse à des stimuli environnementaux est une propriété émergente du cerveau. Cependant, la connaissance que nous avons de la nature précise de ces réseaux est encore limitée. Au niveau du cortex visuel, qui est l’aire cérébrale la plus étudiée, la manière dont les informations se transmettent de neurone en neurone est une question qui reste encore inexplorée. Cela nous invite à étudier l’émergence des microcircuits en réponse aux stimuli visuels. Autrement dit, comment l’interaction entre un stimulus et une assemblée cellulaire est-elle mise en place et modulée? Méthodes En réponse à la présentation de grilles sinusoïdales en mouvement, des ensembles neuronaux ont été enregistrés dans la couche II/III (aire 17) du cortex visuel primaire de chats anesthésiés, à l’aide de multi-électrodes en tungstène. Des corrélations croisées ont été effectuées entre l’activité de chacun des neurones enregistrés simultanément pour mettre en évidence les liens fonctionnels de quasi-synchronie (fenêtre de ± 5 ms sur les corrélogrammes croisés corrigés). Ces liens fonctionnels dévoilés indiquent des connexions synaptiques putatives entre les neurones. Par la suite, les histogrammes peri-stimulus (PSTH) des neurones ont été comparés afin de mettre en évidence la collaboration synergique temporelle dans les réseaux fonctionnels révélés. Enfin, des spectrogrammes dépendants du taux de décharges entre neurones ou stimulus-dépendants ont été calculés pour observer les oscillations gamma dans les microcircuits émergents. Un indice de corrélation (Rsc) a également été calculé pour les neurones connectés et non connectés. Résultats Les neurones liés fonctionnellement ont une activité accrue durant une période de 50 ms contrairement aux neurones fonctionnellement non connectés. Cela suggère que les connexions entre neurones mènent à une synergie de leur inter-excitabilité. En outre, l’analyse du spectrogramme dépendant du taux de décharge entre neurones révèle que les neurones connectés ont une plus forte activité gamma que les neurones non connectés durant une fenêtre d’opportunité de 50ms. L’activité gamma de basse-fréquence (20-40 Hz) a été associée aux neurones à décharge régulière (RS) et l’activité de haute fréquence (60-80 Hz) aux neurones à décharge rapide (FS). Aussi, les neurones fonctionnellement connectés ont systématiquement un Rsc plus élevé que les neurones non connectés. Finalement, l’analyse des corrélogrammes croisés révèle que dans une assemblée neuronale, le réseau fonctionnel change selon l’orientation de la grille. Nous démontrons ainsi que l’intensité des relations fonctionnelles dépend de l’orientation de la grille sinusoïdale. Cette relation nous a amené à proposer l’hypothèse suivante : outre la sélectivité des neurones aux caractères spécifiques du stimulus, il y a aussi une sélectivité du connectome. En bref, les réseaux fonctionnels «signature » sont activés dans une assemblée qui est strictement associée à l’orientation présentée et plus généralement aux propriétés des stimuli. Conclusion Cette étude souligne le fait que l’assemblée cellulaire, plutôt que le neurone, est l'unité fonctionnelle fondamentale du cerveau. Cela dilue l'importance du travail isolé de chaque neurone, c’est à dire le paradigme classique du taux de décharge qui a été traditionnellement utilisé pour étudier l'encodage des stimuli. Cette étude contribue aussi à faire avancer le débat sur les oscillations gamma, en ce qu'elles surviennent systématiquement entre neurones connectés dans les assemblées, en conséquence d’un ajout de cohérence. Bien que la taille des assemblées enregistrées soit relativement faible, cette étude suggère néanmoins une intrigante spécificité fonctionnelle entre neurones interagissant dans une assemblée en réponse à une stimulation visuelle. Cette étude peut être considérée comme une prémisse à la modélisation informatique à grande échelle de connectomes fonctionnels. / Background ‘Connectomics’— the mapping of neural connections, is a rapidly advancing field in neurosciences and it promises significant insights into the brain functioning. The formation of neuronal circuits in response to the sensory environment is an emergent property of the brain; however, the knowledge about the precise nature of these sub-networks is still limited. Even at the level of the visual cortex, which is the most studied area in the brain, how sensory inputs are processed between its neurons, is a question yet to be completely explored. Heuristically, this invites an investigation into the emergence of micro-circuits in response to a visual input — that is, how the intriguing interplay between a stimulus and a cell assembly is engineered and modulated? Methods Neuronal assemblies were recorded in response to randomly presented drifting sine-wave gratings in the layer II/III (area 17) of the primary visual cortex (V1) in anaesthetized cats using tungsten multi-electrodes. Cross-correlograms (CCGs) between simultaneously recorded neural activities were computed to reveal the functional links between neurons that were indicative of putative synaptic connections between them. Further, the peristimulus time histograms (PSTH) of neurons were compared to divulge the epochal synergistic collaboration in the revealed functional networks. Thereafter, perievent spectrograms were computed to observe the gamma oscillations in emergent microcircuits. Noise correlation (Rsc) was calculated for the connected and unconnected neurons within these microcircuits. Results The functionally linked neurons collaborate synergistically with augmented activity in a 50-ms window of opportunity compared with the functionally unconnected neurons suggesting that the connectivity between neurons leads to the added excitability between them. Further, the perievent spectrogram analysis revealed that the connected neurons had an augmented power of gamma activity compared with the unconnected neurons in the emergent 50-ms window of opportunity. The low-band (20-40 Hz) gamma activity was linked to the regular-spiking (RS) neurons, whereas the high-band (60-80 Hz) activity was related to the fast-spiking (FS) neurons. The functionally connected neurons systematically displayed higher Rsc compared with the unconnected neurons in emergent microcircuits. Finally, the CCG analysis revealed that there is an activation of a salient functional network in an assembly in relation to the presented orientation. Closely tuned neurons exhibited more connections than the distantly tuned neurons. Untuned assemblies did not display functional linkage. In short, a ‘signature’ functional network was formed between neurons comprising an assembly that was strictly related to the presented orientation. Conclusion Indeed, this study points to the fact that a cell-assembly is the fundamental functional unit of information processing in the brain, rather than the individual neurons. This dilutes the importance of a neuron working in isolation, that is, the classical firing rate paradigm that has been traditionally used to study the encoding of a stimulus. This study also helps to reconcile the debate on gamma oscillations in that they systematically originate between the connected neurons in assemblies. Though the size of the recorded assemblies in the current investigation was relatively small, nevertheless, this study shows the intriguing functional specificity of interacting neurons in an assembly in response to a visual input. One may form this study as a premise to computationally infer the functional connectomes on a larger scale.

The goal of this thesis is to investigate the functional role of the cortical column architecture within some of the existing brain coding theories. Here I focus on the hierarchical models of predictive coding and the 'phase of firing' coding hypothesis. Using an oddball paradigm consisting of a sequence of identical sounds interspersed with rare, unexpected sounds, one can observe a difference between the scalp potentials evoked by oddball and common sounds. This difference has been linked to predictive coding and novelty detection, and Stimulus Specific Adaptation (SSA) has been suggested as a likely substrate at the single neuron level. In order to simultaneously constrain hierarchical models of predictive coding, and so as to investigate the contributions that neural processing within the different cytoarchitectonic layers of the primary auditory cortex (A1) may make to SSA, I simultaneously recorded multi-unit activity and current source density (CSD) profiles across all layers in A1 of the rat in response to standard and oddball tones. Our results suggest that SSA arises at the level of the thalamocortical synapse and is further enhanced in the supragranular layers. The phase of low-frequency Local Field Potentials (LFPs) in primary sensory cortices carries stimulus related information and disambiguates the information about different stimuli evoking similar spike rates. However, it is yet unclear how these informative LFP phase values arise within the laminar organization of cortical columns. To address this issue, I performed CSD recordings in the area A1 of anaesthetized rats during the presentation of complex naturalistic sounds. Information theoretic analysis revealed that most LFP phase information originates from discrete CSD events consisting of strong granular-superficial-layer dipoles, likely triggered by bursts of thalamocortical activation. These events, which occur at rates of 2-4 Hz, reliably reset LFP phases at times of strong network excitation. They therefore provide a useful reference frame to measure neural activity with respect to salient times of stimulus history. CSD events display a diverse, stimulus-dependent morphology: these reflect the outcomes of cortical computations which result in varying extents of activation of infragranular output layers.

HIV is capable of causing a set of neurological diseases collectively termed the HIV Associated Neurocognitive Disorders (HAND). Worsening pathology is observed in HIV+ individuals who use opioid drugs. Memory problems are often observed in HAND, implicating HIV pathology in the hippocampus, and are also known to be exacerbated by morphine use. HIV-1 Tat was demonstrated to reduce spatial memory performance in multiple tasks, and individual subsets of CA1 interneurons were found to be selectively vulnerable to the effects of Tat, notably nNOS+/NPY- interneurons of the pyramidal layer and stratum radiatum, PV+ neurons of the pyramidal layer, and SST+ neurons of stratum oriens. Each of these interneuron subsets are hypothesized to form part of a microcircuit involved in memory formation. Electrophysiological assessment of hippocampal pyramidal neurons with Tat and morphine together revealed that Tat caused a reduction in firing frequency, however, chronic morphine exposure did not have any effect. When morphine was removed after chronic exposure, non-interacting effects of Tat and morphine withholding on firing frequency were observed, suggesting that a homeostatic rebalancing of CA1 excitation/inhibition balance takes place in response to chronic morphine exposure independently of any Tat effects. Additionally, differential morphological effects of Tat and morphine were observed in each of the three major dendritic compartments, with SR being less affected, suggesting complex circuit responses to these insults reflecting local change and potentially changes in inputs from other brain regions. Behaviorally, Tat and morphine interactions occur in spatial memory, with morphine potentially obviating Tat effects.