Magnetické vortexy jsou charakterizovány směrem stáčení magnetizace a polarizací vortexového jádra, přičemž každá z těchto veličin nabývá dvojice stavů. Ve výsledku jsou tak k dispozici čtyři možné stabilní konfigurace, čehož může být využito v multibitových paměťových zařízeních. Tato dizertační práce se zabývá selektivním zápisem stavů magnetického vortexu v magnetickém disku pulzem elektrického proudu stejně jako jejich následným elektrickým čtením. Před samotnou realizací elektrických měření byla provedena statická měření přepínání stavů vortexu pomocí různých proudových pulzů v kombinaci s technikami MFM a následně MTXM. Následně byl realizován dynamický odečet stavu vortexu kompletně založený na elektrických měřeních. Ovládání cirkulace vortexu je založeno na geometrické asymetrii vytvořené oříznutím magnetického disku a vytvořením fazety. Plochý okraj disku definuje preferenční smysl stáčení cirkulace během procesu nukleace vortexu. Řízení polarity se obvykle provádí ve dvou krocích. V prvním kroku, homogenně magnetizovaná vrstva s kolmou magnetickou anizotropií umístěná na dně disku definuje výchozí polaritu vortexu v době nukleace. V druhém kroku, je-li to nutné, je polarita vortexu přepnuta pomocí rychlého proudového pulzu. Proto je možné nastavit požadovaný stav cirkulace vysláním nanosekundového pulsu s nízkou amplitudou, následované nastavením polarity pikosekundovým pulsem s vysokou amplitudou. Stavy vortexů jsou pak detekovány elektrickou spektroskopií prostřednictvím anizotropní magnetorezistence. Vzorky pro všechna statická a dynamická měření byly připraveny pomocí elektronové litografie v kombinaci s lift-off procesem.

This thesis deals with optical distance measurement and 3D scene measurement using focusing techniques with focus on confocal microscopy, depth from focus and depth from defocus. Theoretical part of the thesis is about different approaches to depth map generation and also about micro image defocusing technique for measuring refractive index of transparent materials. Then the camera calibration for focused techniques is described. In the next part of the thesis is described experimentally verification of depth from focus and depth from defocus techniques. For the first technique are shown results of depth map generation and for the second technique is shown comparison between measured distance values and real distance values. Finally, the discussed techniques are compared and evaluated.

This diploma thesis deals with welding of two dissimilar materials by laser. The samples were welded by direct beam guidance and wobbling, with and without using high alloy filler material and with the use of a diffusion barrier in the form of nickel. Welding was done with different shielding atmospheres. Nondestructive (VT, PT, UT-PA, RT) and destructive tests were performer (tensile test at room and elevated temperatures, microhrardness). Metalography was suplemented by EDX analysis of selected elements.

This diploma thesis deals with the issue of motility analysis in leukemia cells. An accurate description of the cell movement and the detection of differences in motility under experimental conditions can be obtained by quantitative analysis of cell motility using time-lapse recording. The first part of this work describes various types of tumor cell migraton. The second part focuses on methods of analysis of cell motility in tissue culture using time-lapse recording, which include image acquisition and processing. Part of this chapter describes a coherence-controlled holographic microscope, which was used in the practical part and for which an insert was designed to ensure the exact and stable position of the individual chambers. The last part is focused on the research of leukemic cell motility, which is concluded by a discussion of the obtained results. The appendix contains a published study included acknowledgement to the author of this diploma thesis for participation in the project.

Studium chování opticky zachycených částic nám umožňuje porozumět základním fyzikálním jevům plynoucím z interakce světla a hmoty. Předkládaná práce podává vysvětlení zesílení tažné síly působící na opticky svázané částice ve strukturovaném světelném poli, tzv. tažném svazku. Ukazujeme, že pohyb dvou opticky svázaných objektů v tažném svazku je silně závislý na jejich vzájemné vzdálenosti a prostorové orientaci, což rozšiřuje možnosti manipulace hmoty pomocí světla. Následně se práce zaměřuje na levitaci opticky zachycených částic ve vakuu. Představujeme novou metodologii na charakterizaci vlastností slabě nelinearního Duffingova oscilátoru reprezentovaného opticky levitující částicí. Metoda je založena na průměrování trajektorií s určitou počáteční pozicí ve fázovém prostoru sestávajícím z polohy a rychlosti částice a poskytuje informaci o parametrech oscilátoru přímo ze zaznamenaného pohybu. Náš inovativní postup je srovnán s běžně užívanou metodou založenou na analýze spektrální hustoty polohy částice a za využití numerických simulací ukazujeme její použitelnost i v nízkých tlacích, kde nelinearita hraje významnou roli.

The diploma thesis deals with the determination of thickness and refractive index of thin organic films using image analysis. In the theoretical part there are described principles of the methods, which are used to prepare the films (spin coating, inkjet printing, vapour deposition), the characteristics of thin films, ways of finding out the thickness and refractive index of substances (weight methods, electric methods, method based on measurement of absorption coefficient of light, interference microscopy, ellipsometry) and also image analysis (harmonic and wavelet analysis). Interference microscope Epival - Interpako (Carl Zeiss Jena), digital camera Nikon Coolpix 5400 and computer were used for the determination of thickness and refractive index. The thicknesses of layers were set on the basis of interference images of edges and grooves – both from the side of the metal contact and the side of underlying glass. The refractive indices of thin layers were then set using the recorded figures. In the final part of the thesis there are discussed the results of interference images photographed along the full length of the aluminium contact which are used for measuring electrical characteristics of DPP structures. The produces are thicknesses and refractive indices of individual layers.

This master’s thesis deals with a design and optimization of an experimental scintillation secondary electron detector for the environmental scanning electron microscope and with a description of a detector operation principle. The experiment is founded on simulations of a gas flow in detector inner sections and on simulations of secondary electron trajectories in electrostatic fields of the detector. On the basis of the simulations, new solutions of the detector designs are proposed. For these designs, same simulations as previous are performed and designs that seem to be feasible for the secondary electron detection in environmental scanning electron microscope are selected.

The thesis deals with the natural substances in context with the cancer disease. The natural substances have a positive effect on the human organism and they are able to influence the viability and the growth of the cancer cells. The main mechanical device is to influence the mechanisms needed to start the apoptosis of the cancer cells and stopping further proliferation. The cancer cell lines utilization in the cancer disease is discussed in the thesis too. The thesis states common methods of determining the natural substances cytotoxicity. For the experimental part of the thesis it was chosen the MTT test method and the xCELLigence system for monitoring in real time. The mechanical device of the tested substance capsaicin in application on the prostate cell lines, tumorous PC3 and nontumorous PNT1A influence will be observed within the experimental part of the thesis.

The aim of this work is to make a literature search in the measurement of membrane voltage using the voltage-sensitive dyes and suggest a method of measuring the membrane voltage on the available cells using the voltage-sensitive dye Di-4-ANEPPS and RH237. The work contains an introduction to electrophysiology of cell, explains fluorescence and typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document was largely devoted to characterization and distribution of voltage-sensitive dyes. The output of a design solution is a real experiment.

The present thesis deals with optimization of the formation of thin layers of metallic materials. In the theoretical part was elaborated overview of the implementation of the formation of thin- film structures, particularly using vacuum techniques. Chemical and physical vapor deposition. They were described methods of pretreatment of thin layers. The findings were discussed methods of controlling the pre-treated surface, quality control and mutual adhesion of coating layers. In the experimental part of the pre-treatment methods were selected and applied to selected substrates. Pretreatments were evaluated using a control surface by wetting angles and by atomic force microscopy. Then, the metal structure formed of copper on the pretreated substrates. Implementation by means of magnetron sputtering. Adhesion layers was checked by testing the surface lattice method. The quality was observed by scanning electron microscopy. The results were used to design the technological process of making metal layers.