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Micro analytical observation of elemental distribution in arbuscular mycorrhizal (AM) roots from mining sites in South Africa and identification of their AM fungiZamxaka, Mtutuzeli January 2016 (has links)
A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg,
in fulfilment of the requirements for the degree of Doctor of Philosophy.
Johannesburg, 2016. / South Africa, as one of the leaders in mining industry, due to the variety and quantity of minerals produced, has been and is still producing a number of mine tailings which are contaminated by heavy metals. Heavy metals are very harmful to plants and especially to human beings and animals due to their non-biodegradable nature. The problem of environmental metal pollution could be combated by the establishment of Arbuscular Mycorrhiza (AM) vegetation on the surface of mine tailings. Besides the toxicity of the substrate, such areas usually lack essential nutrients (mainly N, P, and K) and organic matter. AM fungi contribute to soil structure by forming micro- and macro- soil aggregates within the net of external hyphae. Their presence may reduce stress caused by lack of nutrients or organic matter and increase plant resistance to pathogens, drought and heavy metals. Therefore, mycorrhizal fungi may become the key factor in successful plant revegetation of heavy-metal-polluted areas by promoting the success of plant establishment and increasing soil fertility and quality.
The aim of this project was to identify AM fungi from a number of heavy metal sites in South Africa using both morphological and molecular techniques, followed by the evaluation of heavy metal distribution and localisation in mycorrhizal roots. Soil samples were collected from three different provinces, namely: Gauteng, Mpumalanga and North West provinces. The sites were selected based on their historical and current heavy metal contamination. Indigenous AM fungal isolates (which are adapted to local soil conditions) can stimulate plant growth better than non-indigenous isolates. AM fungal spores were isolated from 100g of representative soil sample by the wet sieving and decanting method, followed by assessment of spore numbers and infective propagules. The spores of a subset of the pot samples were mounted on microscope slides in polyvinyl lactic acid glycerol and identified by morphological characteristics to the level of genus or species. Most of the spores counted were observed in a 45 μm sieve. These spores were tiny and had different sizes, colours and shapes. The majority of the observed spores were small, brown
and oval in shape. For morphological identification, plant roots were stained and hyphae were found to be the most abundant in roots.
For molecular identification, two sets of nested PCR primers, namely NS1 & NS4 coupled with AML1 & AML2, were employed in this study due to their ability to amplify all subgroups of arbuscular mycorrhizal fungi (AM fungal, Glomeromycota), while excluding sequences from other organisms. Through both morphological characteristics and molecular identification, the following fungal genera were identified for the first time in the studied sites in South Africa. The study identified a total of 14 AM fungal genera and 55 AM fungal species, which are: Glomus (15), Acaulospora (11), Scutellospora (6), Gigaspora (6), Rhizophagus (3), Funneliformis (3), Archaeospora (2), Claroideoglomus (2), Ambispora (2), Sclerocystis (1), Fuscutata (1), Entrophospora (1), Diversispora (1), Paraglomus (1). Both Glomus and Acualospora have been observed to be the highest occurring genera in the analysed soil samples, followed by Scutellospora and Gigaspora and others mentioned.
PIXE technique was successful in localising elemental concentration in both plant roots and AM fungal structures, as well as in indicating the large vesicles in root tissue. AM fungal structures in the outer cortex or outer epidermal layer of the root cross-sections were observable, as shown by the more significantly enriched Si in the vesicles and arbuscules. Distinctive elemental maps can be used to localise sites of colonisation and verification of the symbiotic nature of the tissue. This indicates that a range of metals can be sequestered in AM fungal structures above levels in surrounding host root tissue, and demonstrates the potential of Micro-PIXE to determine metal accumulation and elemental distribution in mycorrhizal plant roots and inter-and intracellular AM fungal structures.
This research highlights the potential of AM fungi for inoculation of plants as a prerequisite for successful restoration of heavy metal contaminated soils. It also illustrates the importance of AM fungal diversity in selected high heavy metal
(HM) sites in RSA, particularly in the North West and the Gauteng gold mining slime dams. Therefore, phytoremediation of mine tailings by mycorrhizal plants seems to be one of the most promising lines of research on mine tailings contamination by heavy metals. The strategies which evolved during this project have great potential for phytoremediation of toxic mining sites, and thus can help mitigate the environmental problems, especially in the mining waste sites. / LG2017
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Seismological and mineralogical studies of the world’s deepest gold-bearing horizon, the Carbon Leader Reef, West Wits Line goldfields (South Africa): implications for its poor seismic reflective characterNkosi, Nomqhele Zamaswazi January 2016 (has links)
A dissertation submitted to the Faculty of Science, University of the Witwatersrand
in fulfilment of the requirements for the degree of Master of Science, School of Geosciences. Johannesburg, 2016. / The measurements of physical rock properties, seismic velocities in particular, associated with ore
deposits and their host rocks are crucial in interpreting seismic data collected at the surface for
mineral exploration purposes. The understanding of the seismic velocities and densities of rock units
can help to improve the understanding of seismic reflections and thus lead to accurate interpretations
of the subsurface geology and structures. This study aims to determine the basic acoustic properties
and to better understand the nature of the seismic reflectivity of the world’s deepest gold-bearing reef,
the Carbon Leader Reef (CLR). This was done by measuring the physical properties (ultrasonic
velocities and bulk densities) as well as conducting mineralogical analyses on drill-core samples.
Ultrasonic measurements of P- and S-wave velocities were determined at ambient and elevated
stresses, up to 65 MPa. The results show that the quartzite samples overlying and underlying the
CLR exhibit similar velocities (~ 5028 m/s-5480 m/s and ~ 4777 m/s-5211 m/s, respectively) and bulk
densities (~ 2.68 g/cm3 and 2.66 g/cm3). This is due to similar mineralogy and chemical compositions
observed within the units. However, the CLR has slightly higher velocity (~ 5070 m/s-5468 m/s) and
bulk density (~ 2.78 g/cm3) than the surrounding quartzite units probably due to higher pyrite content
in the reef, which increases the velocity. The hangingwall Green Bar shale exhibits higher velocity
(5124 m/s-5914 m/s) and density values (~ 2.89 g/cm3-3.15 g/cm3) compared to all the quartzite units
(including the CLR), as a result of its finer grain size and higher iron and magnesium content. In the
data set it is found that seismic velocities are influence by silica, iron and pyrite content as well as the
grain size of the samples, i.e., seismic velocities increase with (1) decreasing silica content, (2)
increasing iron and pyrite content and (3) decreasing grain size. Reflection coefficients calculated
using the seismic velocities and densities at the boundaries between the CLR and its hangingwall and
footwall units range between ~0.02 and 0.05, which is below the suggested minimum of 0.06 required
to produce a strong reflection between two lithological units. This suggests that reflection seismic
methods might not be able to directly image the CLR as a prominent reflector, as observed from the
seismic data.
The influence of micro-cracks is observed in the unconfined uniaxial compressive stress tests where
two regimes can be identified: (1) From 0 - 25 MPa the P-wave velocities increase with progressive
loading, but at different rates in shale and quartzite rocks owing to the presence of micro-cracks and
(2) above stresses of ~20 - 25 MPa, the velocity stress relationship becomes constant, possibly
indicating total closure of micro-cracks.
The second part of the study integrates 3D reflection seismic data, seismic attributes and information
from borehole logs and underground mapping to better image and model important fault systems that
might have a direct effect on mining in the West Wits Line goldfields. 3D seismic data have delineated
first-, second- and third-order scale faults that crosscut key gold-bearing horizons by tens to hundreds
of metres. Applying the modified seismic attribute has improved the imaging of the CLR by
sharpening the seismic traces. Conventional interpretation of the seismic data shows that faults with
throws greater than 25 m can be clearly seen. Faults with throws less than 25 m were identified
through volumetric (edge enhancement and ant-tracking seismic attributes) and horizon-based (dip,
dip-azimuth and edge detection seismic attributes) seismic attribute analysis. These attributes
provided more accurate mapping of the depths, dip and strikes of the key seismic horizon
(Roodepoort shale), yielding a better understanding of the relationship between fault activity, methane
migration and relative chronology of tectonic events in the goldfield. The strato-structural model
derived for the West Wits Line gold mines can be used to guide future mine planning and designs to
(1) reduce the risks posed by mining activities and (2) improve the resource evaluation of the goldbearing
reefs in the West Wits Line goldfields. / LG2017
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"A legal analysis of the Mineral and Petroleum Resources Development Act (MPRDA) 28 of 2002" and its impact in the Limpopo Province"Ramatji, Kanuku Nicholas January 2013 (has links)
Thesis (LLM. (Development and Management Law)) -- University of Limpopo, 2013 / In terms of the previous mining legislation in South Africa, mineral rights were held privately and in some instances by the state. The Mineral and Petroleum Resources Development Act (MPRDA) now vests all mineral rights in the state. Through the transitional provisions included in the MPRDA, mining companies can convert their existing ‘old order’ rights to prospect and/or mine (previously granted under the now repealed Minerals Act) to the ‘new order’ rights introduced by the MPRDA. The purpose of the MPRDA is to ensure the sustainable utilisation of South Africa’s mineral and petroleum resources within a national environmental framework policy which primarily protects sensitive environments and the interests of affected communities, organisations and individuals, while promoting socio-economic development.
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The applicability of passive treatment systems for the mitigation of acid mine drainage at the Williams Brothers Mine, Mariposa County, California: bench- and pilot-scale studies /Clyde, Erin Jane. January 1900 (has links) (PDF)
Thesis (MSc., Geology) -- Queen's University, 2008. / Includes bibliographical references.
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The placer deposits in the Upper Kougarok, Seward Peninsula, Alaska /Shallit, A. B. January 1900 (has links) (PDF)
Thesis (Engineer of Mines)--University of Alaska. Thesis summarizes survey of geology, geography, access, gold analysis and type classification, and economic potential of development on the Seward Peninsula Mining Region, Bendeleben Quadrangle. / Alaska "Territorial Department of Mines reports" or "TDM reports" are a collection of reports, notes and maps written by Dept. employees working out of several field offices throughout the territory. Series titles and numbers within the collection were retrospectively assigned with the exception of the few written after statehood (1959). Preliminary leaf is a letter from the author dated "April 29, 1941." Plate I "Outline map of Seward Peninsula showing location of Taylor Region," 1:150,000, sheet unfolds to 22 x 30 cm.; plate II, "Topographic map of Taylor vicinity," 1:12,000, sheet unfolds to 39 x 41 cm.; plate III "Detail sketch Placer deposits in vicinity of Taylor, Seward Peninsula, Alaska," 1:4800, sheet unfolds to 34 x 68 cm.; plate IV Topography - Geology drainage of a part of the Upper Kougarok River," 1 inch = 1.6 miles, sheet unfolds to 68 x 59 cm.; plate V "Cross section sketch - Structure along lines A-B, C-D, E-F," scale varies, sheet unfolds to 38 x60 cm.; plate VI "Contour sketch map surface and bedrock," 1:9600, sheet unfolds to 39 x 41 cm.; and plate VII "Sections along lines shown on Plate II - Kougarok River Bench Placers," scale varies, sheet unfolds to 59 x 45 cm. Includes bibliographical references (leaf [29]). Also available in electronic format via Internet.
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Indigenous development and self-determination in West Papua : socio-political and economic impacts of mining upon the Amungme and Kamoro communities of West Papua /Hisada, Toru. January 2008 (has links)
Thesis (M.A.)--University of Waikato, 2007. / Originally presented as the author's M.A thesis, Waikato University, 2008.
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The importance of integrated sustainability reporting to the mining industry in Namibia.Eagleton, Stephen. January 2013 (has links)
M.Tech. Internal Auditing / Mining can have a positive impact on Namibia by contributing towards economic growth and infrastructure development, by creating jobs, and by increasing foreign currency earnings; but on the other hand, the exploration of natural resources often runs in parallel with corruption, exploitation, environmental devastation, and population displacement through migrant labour. It is therefore imperative for mines to be sustainable in their operations: in other words, mines must protect and enhance the economic, social and environmental milieus of Namibia. The main objective of a mining company is profit maximisation through exploitation of natural resources, and the company can therefore be defensive, even antagonistic, towards sustainable operations and sustainability reporting. The pressure from mining companies' stakeholders for them to be transparent in their operations, and to report on all sustainability-related issues is increasing, and research has indicated that mining companies are, in the majority, supportive of the initiative, and realise the advantages of pursuing sustainable operations. Research results from the study indicated that between the different types of companies, and between countries, there is a significant variation in the sustainability reporting performance, both delivered and required. Improvement opportunities were identified in a number of areas with regards to the quality, consistency and extent of sustainability reporting. The rationale and the motivation for this study was therefore to determine the standard of integrated sustainability reporting being achieved by mining companies in Namibia, in order to determine the needs and shortcomings in the sustainability reporting arena. The final deliverable of this study was to develop a reporting framework which could enable enhanced integrated sustainability reporting by providing the mining industry in Namibia with a structured framework that conforms to world class standards.
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The structural geology of the Yauli Dome Region, Cordellera Occidental, PeruLepry, Louis Anthony, Jr. January 1981 (has links)
No description available.
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Mineralogy, zoning, and paragenesis of sulfide ores at the Ground Hog mine, central district, New MexicoCatlin, Steven Allen January 1981 (has links)
No description available.
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Response of the Black Mountain, South Africa, sulfide deposit to various geophysical techniques and implications for exploration of similar depositsStevenson, Frederick January 1985 (has links)
No description available.
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