Research on network traffic measurement is becoming popular because it could find the reason and valuable information regarding the occurrence of timestamp accuracy error. In this thesis the performance of UDP traffic between application level and link level has been investigated on three different Smartphone operating systems. One Java ME traffic generator and sink was developed for this thesis work. Distributed Passive Measurement Infrastructure with Measurement Point was used for the experiment. To get high timestamp accuracy DAG 3.5E card was used which was synchronized with GPS. Through the different tests under different protocol layers, it can be concluded that the Android mobile performs better as Java ME application works like a native application, whereas Symbian and Windows mobile struggles with incompatible implementation for poor JVM support. Time stamping at link level is more accurate and far different from application level. This thesis also investigated the performance evaluation of different JVM for Windows mobile.