Shallow lakes in Muskoka-Haliburton have been largely ignored in previous limnological and paleolimnological studies, as many are considered to be less desirable for cottage development and other cultural activities. Nonetheless, shallow lakes offer important habitat for many animals. 30 oligo- and mesotrophic, shallow lakes were chosen for a paleolimnological study to assess the impacts of multiple stressors on cladoceran invertebrates. Fossil cladoceran remains preserved in the sediment samples of the study lakes were used to evaluate the nature and magnitude of any changes in assemblages from both modern and pre-industrial times. Relationships between present-day assemblages and key environmental variables were investigated using redundancy analysis, which identified that lake area (p<0.01) and Secchi depth (p<0.05) were significant predictors of assemblage composition in the shallow lakes. Secchi depth was not correlated to water clarity measures, as it usually is, but rather to macrophyte abundance. The modern-day assemblages were compared to the pre-industrial assemblages using the snapshot “top-bottom” paleolimnological approach. The top and bottom assemblages were compared using an ANOSIM which was not significant (p=0.2), confirming that modern assemblages were similar to pre-industrial assemblages in shallow lakes. Full core analysis from three shallow lakes also showed only subtle changes in littoral assemblage composition through time, further supporting results from the top/bottom study. The changes recorded were mainly in the pelagic taxa, with the littoral taxa relatively very stable through time. These small, shallow lakes were then compared and combined with a deep lake set from the same region. The results show some striking differences between shallow and deep lakes. For example, pelagic taxa appear to be driving changes within assemblages across a depth gradient, with most of the changes in the deeper lakes. Water chemistry has an increasingly important role in structuring cladoceran assemblage as lakes become deeper, although lake morphometry does play an important role in defining cladoceran assemblages in all the study lakes. Cladoceran assemblages in shallow lakes appear to be more stable than deeper lakes, despite being exposed to the same regional stressors, such as acidification, calcium decline, and climate change. / Thesis (Master, Biology) -- Queen's University, 2012-12-31 13:41:52.662

Decreases in ocean pH through ocean acidification has shown to have direct negative impacts on the early life stages of the Pacific purple sea urchin, Strongylocentrotus purpuratus. Research has suggested that multiple stressors could exacerbate, cancel, or even alleviate the impacts of ocean acidification on echinoderms. This study assessed the combined effects of changes in pCO2 concentrations (390, 800, 1500 ppm), salinities (28, 31, 34 ppt) and temperatures (12, 15, 18°C) on fertilization and larval development in S. purpuratus. Increased pCO2 was the predominant stressor, with additive and antagonistic effects from temperature changes, and no effect from salinity changes. Stressor combinations significantly decreased the rate of normal larval development by 28 – 68%, whereas fertilization and larval survival were unaffected. The strong impact on normal larval development likely indicates that later development stages could be detrimentally affected and could influence the population dynamics of S. purpuratus.

Amphibian populations are rapidly declining worldwide. Although individual factors may have large local influences, worldwide declines are attributed to interacting global stressors including rising temperatures and environmental pollutants. Globally, water temperatures closely track rising air temperatures and increase the metabolic rates of aquatic ectotherms, changing the rates of uptake, biotransformation, and excretion of contaminants, such as metals. To test how interactions of multiple, chronic stressors affect amphibian growth, I exposed Cope's gray treefrogs (Hyla chrysoscelis) in an outdoor mesocosm facility to three temperature regimes (ambient, ambient +1.5 oC, ambient +2.5 oC) in filtered lake water amended with mixtures of cadmium, copper, and lead ranging from 3.7 to 26.7 risk quotients (relative to the chronic concentration criteria protective of freshwater organisms). Temperature shifts and sublethal metals concentrations significantly affected the energetics of tadpoles as assessed by non-parametric and parametric analyses. Regression analyses indicated no significant relationship between temperature and time to forelimb emergence at the three lowest metals concentrations, but the relationship was parabolic at the three highest concentrations, indicating a differential effect of temperature across a gradient of water quality. The concept that tadpoles increase developmental rates to escape unfavorable aquatic conditions was supported at each temperature by shortened time to forelimb emergence (Gosner Stage 42) at the second highest metals concentration. This response, however, was overwhelmed by the energetic costs associated with the highest concentration and the highest temperature. Moreover, these parabolic relationships match predictions based on species specific thermal windows and pejus temperatures. My findings indicate that global warming may affect contaminated waters to a greater extent than pristine aquatic ecosystems, and that the primary effect may be diminished pejus and critical thermal maximum temperatures, and a narrowing of the species' thermal window.

Sensory pollution from artificial night-lighting and anthropogenic noise have increased at a dramatic rate over the last several decades. Alterations to the sensory environment have been found to affect wildlife in a wide variety of ways including behavioral changes, physiological responses, changes in species interactions, and altered community structure. Increased levels of light and noise pollution can originate from many sources including roads, energy development and infrastructure, and urbanization. Even remote or protected areas are not immune to the effects of increased sensory disturbances with 63 percent of protected areas within the United States found to have been exposed to a doubling of background noise levels due to anthropogenic activity and skyglow, the scattering of artificial light by the atmosphere, extending hundreds of kilometers from the source. Despite a large body of work investigating the effects of light or noise pollution acting alone, relatively few studies have examined the effects of both stimuli acting together even though they frequently co-occur. Better understanding how these stressors, especially when present simultaneously, are affecting ecosystems is critical to ongoing mitigation and conservation efforts. In Chapter 1, we investigated the effects of increased levels of light and noise pollution, both singularly and in tandem, on pinyon mouse (Peromyscus truei) activity and body condition. Using a full factorial study design allowed us to isolate the effects of both stimuli when acting alone as well as any potential interactions between the two when both were present. We used standard trapping methods across a gradient of light, noise, and both combined while also accounting for variations in moonlight, vegetative structure, and weather. We found that an increased level of artificial night-lighting resulted in lower trap success of pinyon mice while there was no effect of noise on trap success. There was no effect of elevated light levels on body condition but there was a negative effect of noise on body condition early in the season. Later in the season, neither light nor noise influenced body condition. No interactive effects between light and noise were found. In Chapter 2, we studied the effects of anthropogenic light and noise, singularly and in tandem, on species richness and community structure using camera traps in a manipulative field experiment. We investigated these effects at both the species level and the taxonomic level (nocturnal mammals, diurnal mammals, lagomorphs, birds, mesocarnivores, and ungulates). We showed that both light and noise pollution did alter species richness and that these effects can differ depending on the scale of observation. Increased levels of night-lighting had a scale-dependent effect on species richness such that increases in light levels had a negative effect on richness at the camera level, but light-treated sites had the highest estimated cumulative richness. In contrast, noise was found to have a negative effect on richness for birds. When both stimuli were present, the addition of night-lighting mitigated the effects of noise for birds. For community structure, noise-treated sites were the most dissimilar from other treatments, indicating that increased levels of anthropogenic noise likely have the largest effect on community structure in this study. We also found evidence of a possible rescue effect of light that counteracts the negative effect of noise. That is, combined treatment sites were significantly dissimilar from both light and noise sites but not from the control sites. Together, our results provide evidence that alterations to the sensory environment from anthropogenic activity can affect wild animal populations in multiple ways. As human development increases to meet the demands of growing human populations, more ecosystems will be exposed to increased levels of sensory disturbance, making the understanding of how these changes affect wildlife critical to ongoing conservation efforts.

No description available.

Estressores múltiplos, decorrentes de atividades antropogênicas, podem gerar efeitos combinados em organismos marinhos. Efeitos da acidificação oceânica associados a outros impactos antrópicos são pouco compreendidos, especialmente em zonas costeiras. Nestas regiões, o sombreamento causado por infraestruturas, como portos, pode potencialmente interagir com a redução do pH da água do mar e afetar populações de crustáceos. O objetivo foi avaliar os efeitos de pH reduzido e sombreamento na mortalidade, crescimento, calcificação e respostas comportamentais de deslocamento a odores de predadores vivos (sinal de perigo) e gastrópodes mortos (sinal de recurso disponível) usando juvenis do ermitão Pagurus criniticornis, coletados na Baía do Araçá (São Paulo, SE Brasil). Após 98 dias de experimento, os estressores apresentaram interação significativa sobre a mortalidade dos organismos, além de efeito aditivo sobre o crescimento. A calcificação não diferiu entre tratamentos, indicando que indivíduos foram capazes de manter a calcificação em condições de pH reduzido. Quando expostos a odor de predadores, o deslocamento dos ermitões foi afetado somente pelo sombreamento. Entretanto, uma interação entre ambos os estressores foi observada para o odor de gastrópodes, causando a redução do deslocamento. Este estudo mostra como impactos locais podem amplificar efeitos de mudanças ambientais globais em populações de crustáceos do entremarés. / Multiple stressors due to anthropogenic activities may cause combined impacts in marine organisms. Effects of ocean acidification associated with other anthropogenic impacts are poorly understood, especially in coastal regions. In these areas, shading caused by infrastructure development, such as harbor construction, may potentially interact with CO2-induced pH reduction and affect invertebrate populations. Here we evaluated reduced pH and shading effects on mortality, growth, calcification and displacement behavior to live predator (danger signal) and dead gastropod (resource availability signal) odors using juveniles of the hermit crab Pagurus criniticornis collected in Araçá Bay (São Paulo state, SE Brazil). After a 98 days experiment period, both stressors had a significant interaction on mortality and an additive effect on total growth. No difference in calcification was recorded among treatments, indicating that individuals were able to maintain calcification under reduced pH. When exposed to odor of live predators, crabs\' responses were affected only by shading. However, an interactive effect between both stressors was observed in response to gastropod odor, leading to reduced displacement behavior. This study shows how local disturbance impacts may enhance effects of global environmental changes on intertidal crustacean populations.

Estressores múltiplos, decorrentes de atividades antropogênicas, podem gerar efeitos combinados em organismos marinhos. Efeitos da acidificação oceânica associados a outros impactos antrópicos são pouco compreendidos, especialmente em zonas costeiras. Nestas regiões, o sombreamento causado por infraestruturas, como portos, pode potencialmente interagir com a redução do pH da água do mar e afetar populações de crustáceos. O objetivo foi avaliar os efeitos de pH reduzido e sombreamento na mortalidade, crescimento, calcificação e respostas comportamentais de deslocamento a odores de predadores vivos (sinal de perigo) e gastrópodes mortos (sinal de recurso disponível) usando juvenis do ermitão Pagurus criniticornis, coletados na Baía do Araçá (São Paulo, SE Brasil). Após 98 dias de experimento, os estressores apresentaram interação significativa sobre a mortalidade dos organismos, além de efeito aditivo sobre o crescimento. A calcificação não diferiu entre tratamentos, indicando que indivíduos foram capazes de manter a calcificação em condições de pH reduzido. Quando expostos a odor de predadores, o deslocamento dos ermitões foi afetado somente pelo sombreamento. Entretanto, uma interação entre ambos os estressores foi observada para o odor de gastrópodes, causando a redução do deslocamento. Este estudo mostra como impactos locais podem amplificar efeitos de mudanças ambientais globais em populações de crustáceos do entremarés. / Multiple stressors due to anthropogenic activities may cause combined impacts in marine organisms. Effects of ocean acidification associated with other anthropogenic impacts are poorly understood, especially in coastal regions. In these areas, shading caused by infrastructure development, such as harbor construction, may potentially interact with CO2-induced pH reduction and affect invertebrate populations. Here we evaluated reduced pH and shading effects on mortality, growth, calcification and displacement behavior to live predator (danger signal) and dead gastropod (resource availability signal) odors using juveniles of the hermit crab Pagurus criniticornis collected in Araçá Bay (São Paulo state, SE Brazil). After a 98 days experiment period, both stressors had a significant interaction on mortality and an additive effect on total growth. No difference in calcification was recorded among treatments, indicating that individuals were able to maintain calcification under reduced pH. When exposed to odor of live predators, crabs\' responses were affected only by shading. However, an interactive effect between both stressors was observed in response to gastropod odor, leading to reduced displacement behavior. This study shows how local disturbance impacts may enhance effects of global environmental changes on intertidal crustacean populations.

Non-indigenous species can profoundly alter the ecosystems they invade and impact local economies. Growth and body condition declines of commercially fished Great Lakes lake whitefish coincide with the establishment of non-native dreissenid mussels and the cladoceran Bythotrephes longimanus. Declines in lake herring abundance—a key prey item for other commercially important species—have also been reported. Though additional stressors such as climate change may have contributed to changes in coregonid populations, they have not been thoroughly evaluated. Here, I present data that condition and contaminant declines in coregonids are associated with increasing density or warming climate, but growth declines in lake whitefish are likely due to ecosystem changes associated with dreissenids and Bythotrephes. In South Bay, Lake Huron, changes in lake whitefish diet composition and stable isotope signatures were consistent with increased reliance on nearshore resources after dreissenid establishment; lake whitefish occupied shallower habitats and experienced declines in mean diet energy densities post-dreissenid invasion. Growth of South Bay lake whitefish declined after environmental effects were statistically removed, whereas condition declines were explained best by changes in lake whitefish density. Among four lake whitefish populations, growth declined after dreissenids established, but not in uninvaded reference populations. Growth also declined among four lake whitefish populations after the establishment of Bythotrephes relative to reference populations. In contrast with growth, condition of lake whitefish did not change as a result of dreissenid or Bythotrephes invasion. Bioenergetic models revealed that activity rates increased and conversion efficiencies decreased in lake whitefish populations exposed to dreissenids, despite higher consumption rates in populations with dreissenids present. Condition declines among many lake whitefish and lake herring populations (and declines in mercury among herring populations) reflected regional differences and were not related to the presence of Bythotrephes or Mysis relicta. Declines in condition were more pronounced in northwest Ontario populations where climate has changed more dramatically than in southern Ontario. This work suggests that projected range expansions of dreissenid mussels and Bythotrephes will likely affect native fisheries, and their effect on these fisheries may be exacerbated by declining fish condition associated with climate change.

Non-indigenous species can profoundly alter the ecosystems they invade and impact local economies. Growth and body condition declines of commercially fished Great Lakes lake whitefish coincide with the establishment of non-native dreissenid mussels and the cladoceran Bythotrephes longimanus. Declines in lake herring abundance—a key prey item for other commercially important species—have also been reported. Though additional stressors such as climate change may have contributed to changes in coregonid populations, they have not been thoroughly evaluated. Here, I present data that condition and contaminant declines in coregonids are associated with increasing density or warming climate, but growth declines in lake whitefish are likely due to ecosystem changes associated with dreissenids and Bythotrephes. In South Bay, Lake Huron, changes in lake whitefish diet composition and stable isotope signatures were consistent with increased reliance on nearshore resources after dreissenid establishment; lake whitefish occupied shallower habitats and experienced declines in mean diet energy densities post-dreissenid invasion. Growth of South Bay lake whitefish declined after environmental effects were statistically removed, whereas condition declines were explained best by changes in lake whitefish density. Among four lake whitefish populations, growth declined after dreissenids established, but not in uninvaded reference populations. Growth also declined among four lake whitefish populations after the establishment of Bythotrephes relative to reference populations. In contrast with growth, condition of lake whitefish did not change as a result of dreissenid or Bythotrephes invasion. Bioenergetic models revealed that activity rates increased and conversion efficiencies decreased in lake whitefish populations exposed to dreissenids, despite higher consumption rates in populations with dreissenids present. Condition declines among many lake whitefish and lake herring populations (and declines in mercury among herring populations) reflected regional differences and were not related to the presence of Bythotrephes or Mysis relicta. Declines in condition were more pronounced in northwest Ontario populations where climate has changed more dramatically than in southern Ontario. This work suggests that projected range expansions of dreissenid mussels and Bythotrephes will likely affect native fisheries, and their effect on these fisheries may be exacerbated by declining fish condition associated with climate change.

<p>Aquatic systems are impacted by a wide variety of natural and anthropogenic stressors. Laboratory studies often focus on impacts of a single stressor, ignoring how these stressors may interact. This dissertation focuses on the interactions between polycyclic aromatic hydrocarbons (PAHs) and two physical stressors (hypoxia and solar radiation). PAHs are ubiquitous environmental pollutants that occur in the environment as complex mixtures, the components of which may interact. Some PAHs are agonists of the aryl hydrocarbon receptor (AhR), which regulates expression of a number of genes (such as CYP1A) involved in metabolism, often of the same compounds that induced the AhR. PAHs that are AhR agonists have been shown to interact synergistically with PAHs that inhibit activity of CYP1A, inducing developmental deformities in fish. AhR shares a dimerization partner, the aryl hydrocarbon receptor nuclear translocator (ARNT), with the protein responsible for regulating hypoxia-induced gene expression, hypoxia-inducible factor 1á (HIF1&alpha;), possibly resulting in competition between the two pathways. Competition would result in decreased induction of CYP1A under hypoxic conditions, possibly resulting in synergistic embryonic toxicity between AhR agonist PAHs and hypoxia. In this dissertation, the results are presented of experiments regarding the occurrence of AhR/HIF1&alpha; crosstalk in fish and the developmental consequences of co-exposure to hypoxia and PAHs. In vitro testing revealed competition for ARNT in which HIF1&alpha; appeared to out-compete AhR. Induction of an AhR-responsive luciferase reporter by several AhR agonists (benzo[a]pyrene (BaP), benzo[k]fluoranthene (BkF), and polychlorinated biphenyl (PCB126)) was reduced under hypoxia and this effect was eliminated by overexpression of ARNT. Induction of a HIF1&alpha;-responsive reporter was unaffected by BkF and PCB126. BaP caused an ARNT-independent decrease in both basal and induced HIF1&alpha; reporter activity. Attempts to verify this crosstalk pattern in vivo revealed that BaP-induced CYP1A expression was further increased under hypoxic conditions. Induction of hypoxia-inducible genes VEGF and LDHa were unaffected by BaP. The effect of hypoxia on CYP1A expression was not repeated with BkF or pyrene (PY) and the exact conditions that result in hypoxic changes in CYP1A expression remain to be determined. Embryonic toxicity in zebrafish (Danio rerio), including pericardial edema, heart malformations, and death were synergistically induced by hypoxia and BaP, BkF or complex, environmentally relevant PAH mixtures. There was no effect of hypoxia on PCB126 toxicity and hypoxia protected from the pericardial edema induced by PY. CYP1A knockdown mimicked the effects of hypoxia on BkF and PY toxicity and even further exacerbated BkF toxicity under hypoxic conditions, suggesting a role for metabolism in the toxicity. Additionally, since two CYP1A inhibitors (fluoranthene (FL) and á-naphthoflavone (ANF)) had been previously reported to interact synergistically with hypoxia, three other CYP1A inhibitors (dibenzothiophene, carbazole and 2-aminoanthracene) were tested. None of them induced toxicity in combination with hypoxia, indicating that CYP1A inhibition was not the reason for the interaction of FL and ANF with hypoxia. </p><p>A second stressor known to interact with PAHs to induce toxicity is solar radiation. While most studies of the effects of solar radiation on PAH toxicity have focused on the effects of UV light on PAHs already absorbed by an organism (photoactivation), less attention has been paid to the toxic effects of photomodified PAHs. We exposed carbazole to ambient sunlight and subsequently exposed developing zebrafish to the resulting mixture. Photo-exposed carbazole caused developmental toxicity including edema, heart malformations, craniofacial malformations and death that were not caused by parent carbazole; these effects were severely exacerbated by hypoxia and significant mortality was also observed. Additionally, photo-exposed carbazole induced expression of CYP1A and GSTp, likely resulting from agonism of the AhR and toxicity of this mixture was alleviated by morpholino knockdown of AhR. Some photoproducts were identified, but none of them appeared to be involved in the toxicity or supposed AhR induction observed with photoexposed carbazole. The results of these experiments underscore the importance of consideration of the interactive effects of physical and chemical stressors when assessing risks to wildlife populations inhabiting polluted areas.</p> / Dissertation