by Sze, Lung Yam. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 106-112). / Abstract --- p.i / Acknowledgements --- p.iv / Contents --- p.v / Chapter Chapter 1 --- General Introduction / Chapter 1.1. --- Morphology of the Mammalian Somite and Limb --- p.1 / Chapter 1.1.1. --- The Somite --- p.1 / Chapter 1.1.2. --- The Limb --- p.3 / Chapter 1.2. --- Somite-Limb Relationship --- p.5 / Chapter 1.2.1. --- Somite Contribution to the Appendicular Musculature --- p.5 / Chapter 1.2.2. --- Somite Contribution to Limb Morphogenesis --- p.6 / Chapter 1.3. --- Control of Directionality of Somitic Cell Migration in Appendicular Environment --- p.8 / Chapter 1.4. --- Reasons and Objective of the Present Study --- p.10 / Chapter Chapter 2 --- The Origin of the Mammalian Limb Skeletal Muscles / Chapter 2.1. --- Introduction --- p.16 / Chapter 2.2. --- Materials and Methods --- p.19 / Chapter 2.2.1. --- Embryo Collection --- p.19 / Chapter 2.2.2. --- Isolation of Somites --- p.20 / Chapter 2.2.3. --- DiI-labelling of Rat Donor Somites --- p.21 / Chapter 2.2.4. --- Somite Transplantation --- p.21 / Chapter 2.2.5. --- Embryo Culture --- p.22 / Chapter 2.2.6. --- Analysis of Cultured Embryos --- p.22 / Chapter 2.2.7. --- Cryosection --- p.23 / Chapter 2.2.8. --- Limb Explant Cultures --- p.23 / Chapter 2.2.9. --- Immunohistochemistry --- p.24 / Chapter 2.2.10. --- X-gal Staining --- p.25 / Chapter 2.2.11. --- Histology --- p.25 / Chapter 2.3. --- Results --- p.27 / Chapter 2.3.1. --- Gross Morphology of Cultured Embryos --- p.27 / Chapter 2.3.2. --- Distribution of DiI Labelled Somitic Cells in Rat Embryos --- p.27 / Chapter 2.3.3. --- Histogenetic Potential of Labelled Somitic Cells in the Limbs --- p.29 / Chapter 2.3.4. --- Chimeaic Limb Culture --- p.30 / Chapter 2.4. --- Discussion --- p.32 / Chapter 2.4.1. --- Relationship Between the Somites and the Limb Musculature in Rat Embryos --- p.33 / Chapter 2.4.2. --- Myogenic Potential of Somitic Cells in the Mouse Limb Bud --- p.36 / Chapter 2.4.3. --- The Regulatory Potentials of Mammalian Somites --- p.37 / Chapter Chapter 3 --- The Migration of Somitic Cells into the Mammalian Fore- limb Bud / Chapter 3.1. --- Introduction --- p.52 / Chapter 3.2. --- Materials and Methods --- p.57 / Chapter 3.2.1. --- Embryo Collection --- p.57 / Chapter 3.2.2. --- Embryo Culture and Analysis of Cultured Embryos --- p.58 / Chapter 3.2.3. --- Experimental Series I --- p.58 / Chapter A. --- Micro-injection of DiI --- p.58 / Chapter B. --- Explant Cultures of Rat Fore-limb Bud --- p.59 / Chapter C. --- Histology and Immunohistochemistry --- p.60 / Chapter 3.2.4. --- Experimental Series II --- p.61 / Chapter A. --- Preparation of Conditioned and Unconditioned Medium --- p.61 / Chapter B. --- Coating of Nucleopore Membrane with Fibronectin --- p.62 / Chapter C. --- Preparation of Somitic Cells --- p.62 / Chapter D. --- Analysis of Chemotatic Effect --- p.63 / Chapter 3.2.5. --- Experiment Series III --- p.64 / Chapter A. --- Micro-injection of Latex Beads --- p.64 / Chapter B. --- Isolation of Somatopleure and Transplantation --- p.64 / Chapter C. --- "Somite Isolation, Labelling, and Transplantation" --- p.65 / Chapter D. --- Histology --- p.65 / Chapter 3.3. --- Results --- p.66 / Chapter 3.3.1. --- Development of Embryos In vitro --- p.66 / Chapter 3.3.2. --- Experimental Series I --- p.66 / Chapter A. --- Distribution of Somitic Cells in DiI Injected Embryos --- p.66 / Chapter B. --- Histogenetic Potential of Limb Explants Cultured Under the Kidney Capsule --- p.68 / Chapter C. --- Histogenetic Potential of Limb Explants Cultured In vitro --- p.69 / Chapter 3.3.3. --- Experimental Series II --- p.70 / Chapter A. --- Chemotatic Behaviour of Somitic Cells --- p.70 / Chapter 3.3.4. --- Experimental Series III --- p.71 / Chapter A. --- Ability of Latex Beads to Invade the Limb Bud --- p.71 / Chapter B. --- Distribution Pattern of Somatopleural and Somitic Cells --- p.71 / Chapter 3.4. --- Discussion --- p.75 / Chapter 3.4.1. --- Experimental Series I --- p.75 / Chapter A. --- Distribution of Somitic Cells in DiI-Injected Rat Embryos --- p.75 / Chapter B. --- Histogenetic Potential of Rat Fore-limb Bud --- p.77 / Chapter 3.4.2. --- Experimental Series II --- p.80 / Chapter A. --- Chemotatic Behaviour of Somitic Cells --- p.80 / Chapter 3.4.3. --- Experimental Series III --- p.80 / Chapter A. --- Ability of Latex Beads to Invade the Limb Bud --- p.80 / Chapter B. --- Ability of Somatopleure and Somite to Invade Limb Bud --- p.83 / Chapter 3.4.4. --- Conclusion --- p.83 / References --- p.106 / Appendix --- p.113