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BIOCHEMICAL CHARACTERIZATION OF HUMAN MISMATCH RECOGNITION PROTEINS MUTSα AND MUTSβTian, Lei 01 January 2010 (has links)
The integrity of an organism's genome depends on the fidelity of DNA replication and the efficiency of DNA repair. The DNA mismatch repair (MMR) system, which is highly conserved from prokaryotes to eukaryotes, plays an important role in maintaining genome stability by correcting base-base mismatches and insertion/deletion (ID) mispairs generated during DNA replication and other DNA transactions. Mismatch recognition is a critical step in MMR. Two mismatch recognition proteins, MutSα (MSH2-MSH6 heterodimer) and MutSβ (MSH2-MSH3 heterodimer), have been identified in eukaryotic cells. MutSα and MutSβ have partially overlapping functions, with MutSα recognizing primarily base-base mismatches and 1-2 nt ID mispairs and MutSβ recognizing 2-16-nt ID heteroduplexes. The goal of this dissertation research was to understand the mechanism underlying differential mismatch recognition by human MutSα and MutSβ and to characterize the unique functions of human MutSα and MutSβ in MMR.
In this study, recombinant human MutSα and MutSβ were purified. Binding of the proteins to a T-G mispair and a 2-nt ID mispair was analyzed by gel-mobility assay; ATP/ADP binding was characterized using a UV cross-linking assay; ATPase activity was measured using an ATPase assay; MutSα amd MutSβ’s mismatch repair activity was evaluated using a reconstituted in vitro MMR assay.
Our studies revealed that the preferential processing of base-base and ID heteroduplexes by MutSα and MutSβ respectively, is determined by the significant differences in the ATPase and ADP binding activities of MutSα and MutSβ, and the high ratio of MutSα:MutSβ in human cells. Our studies also demonstrated that MutSβ interacts similarly with a (CAG)n hairpin and a mismatch, and that excess MutSβ does not inhibit (CAG)n hairpin repair in vitro.
These studies provide insight into the determinants of the differential DNA repair specificity of MutSα and MutSβ, the mechanism of mismatch repair initiation, and the mechanism of (CAG)n hairpin processing and repair, which plays a role in the etiology and progression of several human neurological diseases.
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MOLECULAR MECHANISM OF HUMAN MISMATCH REPAIR INITIATIONLee, Sanghee 01 January 2014 (has links)
DNA mismatch repair (MMR) is a highly conserved pathway that maintains genomic stability primarily by correcting mismatches generated during DNA replication. MMR deficiency leads to microsatellite instability (MSI), which is a hallmark of HNPCC (Hereditary Nonpolyposis Colorectal Cancer). Human mismatch repair is initiated by MutSα, a heterodimer of MSH2 and MSH6 subunits. Mismatch binding by MutSα triggers a series of downstream MMR events including interacting and communicating with other MMR proteins. The ATPase domain of MutSα is situated in the C-termini of its both subunits, and ATP binding is required for dissociation of MutSα from a mismatch. In eukaryotic cells, a strand break, which resides either 3’ or 5’ to the mismatch up to several hundred base pair away, determines the strand specificity of MMR. However, in spite of extensive studies, the mechanism by which MutSα locates and senses a nick from the mismatch, and coordinates the subsequent steps of MMR remains poorly understood. Two controversial models have been proposed to explain how the mismatch and the strand break communicate each other. Sliding model proposes that MutSα slides along the DNA helix from the mismatch to the strand break in an ATP binding-dependent but not ATP hydrolysis-dependent manner. Stationary model postulates that MutSα remains bound at the mismatch, and a protein-mediated DNA loop forms, physically bringing the mismatch and the nick in contact. Here, we tested these models in vitro, using a circular plasmid DNA substrate with a single GT mismatch and two Lac repressor (Lac I) binding sites as conditional physical 'roadblocks', one on either side of the mismatch, which when present, prevent MutSα from sliding bi-directionally along the DNA. The results showed that DNA excision initiates under conditions that block MutSα sliding, suggesting that initiation of excision is independent of whether MutSα slides from the mismatch to the nick. This result implies that the communication between the mismatch and the nick is likely through interactions between the mismatch-bound MutSα and other MMR components at the strand break, supporting the stationary model. Therefore, these studies provide significant insight into the mechanisms of mismatch correction in human cells.
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