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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Functional analysis of yeast RAP1

Haw, Robin Andrew January 1999 (has links)
No description available.
2

Mechanisms of drug resistance to acridine derivatives mediated by human DNA topoisomerase IIβ

Leontiou, Chrysoula January 2003 (has links)
No description available.
3

Isolation and characterisation of a locus disrupted in a transgenic mouse mutant exhibiting sex-linked cleft palate

Laverty, Hugh Gerard January 1995 (has links)
No description available.
4

Mutants of Tn3 resolvase

Arnold, Patricia Hazel January 1997 (has links)
No description available.
5

Genetical, molecular and physiological analysis of phytochrome B mutants of Arabidopsis

Bradley, Joy Marie January 1994 (has links)
No description available.
6

Site directed mutagenesis and kinetic study of ferrochelatase from chironomidae larvae.

January 2004 (has links)
Wong Kwong Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 97-104). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / List of Figures --- p.x / List of Tables --- p.xi / List of Abbreviations --- p.xii / Chapter Chapter One --- Introduction --- p.1 / General background and objective of this study --- p.1 / Chapter Part One --- Early studies on ferrochelatase from chironomidae larvae / Chapter 1.1 --- Purification of ferrochelatases from different organisms --- p.5 / Chapter 1.2 --- Purification of ferrochelatase from chironomidae larvae --- p.5 / Chapter 1.3 --- Molecular cloning of ferrochelatase from chironomidae larvae --- p.6 / Chapter 1.4 --- Expression of recombinant chironomidae ferrochelatase --- p.8 / Chapter Part Two --- Study on the iron-sulfur cluster of chironomidae ferrochelatase / Chapter 2.1 --- Presence of iron-sulfur cluster at chironomidae ferrochelatase --- p.13 / Chapter 2.2 --- Possible roles of iron-sulfur cluster at ferrochelatase --- p.13 / Chapter 2.3 --- Mutagenesis study of iron-sulfur cluster at chironomidae ferrochelatase --- p.14 / Chapter Part Three --- Study on the catalytic mechanism of ferrochelatase / Chapter 3.1 --- Binding and distortion of porphyrin --- p.17 / Chapter 3.2 --- Binding of ferrous iron to ferrochelatase --- p.18 / Chapter Part Four --- Study on the effect of metal ions on ferrochelatase / Chapter 4.1 --- Previous studies on the effects of metal ions on different ferrochelatases --- p.24 / Chapter 4.2 --- Effects of metal ions on chironomidae ferrochelatase --- p.24 / Chapter Chapter Two --- Methods and Materials --- p.28 / General procedure --- p.28 / Chapter Part One --- Cloning of chironomidae ferrochelatase coding fragment / Chapter 1.1 --- Amplification of chironomidae ferrochelatase coding cDNA --- p.29 / Chapter 1.2 --- Construction of the expression vector (pETBlue-FECH) --- p.30 / Chapter Part Two --- Mutagenesis / Chapter 2.1 --- Choices of residue replacements --- p.31 / Chapter 2.2 --- Constructions of mutant plasmids --- p.31 / Chapter 2.3 --- Screenings of mutant plasmids --- p.32 / Chapter Part Three --- Construction of chironomidae-human hybrid plasmid / Chapter 3.1 --- Human RNA isolation --- p.33 / Chapter 3.2 --- Human cDNA synthesis --- p.33 / Chapter 3.3 --- Construction of human ferrochelatase expression plasmid (pETBlue-HFECH) --- p.34 / Chapter 3.4 --- Construction of chironomidae-human hybrid plasmid (pETBlue-HYB-FECH) --- p.34 / Chapter 3.4.1 --- First round PCR --- p.34 / Chapter 3.4.2 --- Second round PCR --- p.34 / Chapter 3.4.3 --- Overlapping PCR --- p.35 / Chapter 3.4.4 --- Cloning of HYB-FECH --- p.35 / Chapter Part Four --- Protein expression and purification / Chapter 4.1 --- Protein expression in bacterial expression system --- p.36 / Chapter 4.2 --- Cell harvest and ammonium sulfate fractionation --- p.36 / Chapter 4.3 --- Protein purifications by Blue Sepharose CL-6B chromatography --- p.37 / Chapter 4.4 --- Precipitation of protein samples for SDS-PAGE analysis --- p.37 / Chapter 4.5 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.38 / Chapter 4.6 --- Fast protein liquid chromatography (FPLC) --- p.38 / Chapter Part Five --- Functional study of expressed ferrochelatase / Chapter 5.1 --- Kinetic measurements --- p.40 / Chapter 5.2 --- Metal titration and fluorescence spectroscopy --- p.40 / Chapter Part Six --- Homology modeling of chironomidae ferrochelatase --- p.42 / Chapter Part Seven --- Other routine laboratory methods / Chapter 7.1 --- Determination of nucleic acid --- p.44 / Chapter 7.2 --- Determination of protein --- p.44 / Chapter 7.3 --- Agarose gel electrophoresis of DNA --- p.44 / Chapter 7.4 --- Agarose gel electrophoresis of RNA --- p.45 / Chapter 7.5 --- Nucleic acid purification --- p.45 / Chapter 7.6 --- Preparation of chemically competent bacterial cells --- p.46 / Chapter 7.7 --- Heat shock transformation of competent bacterial cells --- p.46 / Chapter 7.8 --- Colony PCR --- p.47 / Chapter 7.9 --- Plasmid rescue by alkaline lysis --- p.48 / Chapter 7.10 --- Washing of Blue Sepharose CL-6B column --- p.48 / Chapter Chapter Three --- Results --- p.49 / Chapter Part One --- Protein expression and purification --- p.49 / Chapter Part Two --- Kinetic study of chironomidae ferrochelatase --- p.53 / Chapter Part Three --- Mutagenesis study of iron-sulfur cluster --- p.56 / Chapter Part Four --- Mutagenesis study of metalation by ferrous iron / Chapter 4.1 --- Mutagenesis study at His234 --- p.58 / Chapter 4.2 --- "Mutagenesis study at Glu310, Glu313 and Glu317" --- p.58 / Chapter Part Five --- Copper activation of chironomidae ferrochelatase / Chapter 5.1 --- Kinetic analysis of copper activation --- p.62 / Chapter 5.2 --- Copper activation on chironomidae-human hybrid (HYB-FECH) --- p.63 / Chapter 5.3 --- Mutagenesis study on copper activation --- p.63 / Chapter Part Six --- Homology modeling of chironomidae ferrochelatase --- p.70 / Chapter Chapter Four --- Discussion --- p.72 / Chapter Part One --- Protein expression and purification --- p.72 / Chapter Part Two --- Mutant constructions by QuikChange´ёØ approach --- p.73 / Chapter Part Three --- Kinetic study of expressed chironomidae ferrochelatase --- p.74 / Chapter Part Four --- Mutagenesis study of iron sulfur cluster / Chapter 4.1 --- Location of cysteine residues forming the iron-sulfur cluster --- p.77 / Chapter Part Five --- Mutagenesis study of metalation by ferrous iron / Chapter 5.1 --- Role of His234 --- p.79 / Chapter 5.2 --- Catalytic roles of Glu310,Glu313 and Glu317 --- p.80 / Chapter 5.3 --- "Substrate binding on Glu310, Glu313 and Glu317" --- p.83 / Chapter 5.4 --- Partial picture of metalation and future work on active site residues --- p.84 / Chapter 5.5 --- Future works on the active site residues --- p.84 / Chapter Part Six --- Copper activation of chironomidae ferrochelatase / Chapter 6.1 --- Chironomidae ferrochelatase interaction with copper (II) ion --- p.88 / Chapter 6.2 --- Putative copper-binding sites on chironomidae ferrochelatase --- p.89 / Chapter 6.3 --- The hypothesized model of copper activation --- p.90 / Chapter 6.4 --- Direct copper involvement in catalysis --- p.91 / Chapter Chapter Five --- Summary --- p.94 / Chapter Chapter Six --- References --- p.97
7

Mutagenesis of human lymphoblast cells

Kotek, Joseph John January 2011 (has links)
Digitized by Kansas Correctional Industries
8

The basis of restoration mutagenesis in lambda bacteriophage

Elsevier, Susan Marie, January 1970 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1970. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
9

Factors contributing to the formation of CC-->TT tandem mutations /

Skinner, Amy M. January 1900 (has links)
Thesis (Ph. D.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 134-145). Also available on the World Wide Web.
10

Structure variations in lipoxygenase mutagenesis of soybean lipoxygenase-1 /

Imber, Ann Nicole. Gaffney, Betty Jean. January 2006 (has links)
Thesis (M. S.)--Florida State University, 2006. / Advisor: Betty J. Gaffney, Florida State University, College of Arts and Sciences, Dept. of Biological Science. Title and description from dissertation home page (viewed June 7, 2006). Document formatted into pages; contains viii, 52 pages. Includes bibliographical references.

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