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Studies of the mechanism and function of NADH:ubiquinone oxidoreductase (complex I)Sherwood, Steven January 2006 (has links)
No description available.
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The mechanisms of flavin site reactions in NADH:ubiquinone oxidoreductaseBirrell, James Alexander January 2013 (has links)
No description available.
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Understanding the mechanisms of superoxide production by mitochondrial NADH:ubiquinone oxidoreductasePryde, Kenneth Robert January 2013 (has links)
No description available.
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Studies of the catalytic activity of NADH:ubiquinone oxidoreductase (complex I) from bovine mitochondriaSharpley, Mark Stephen January 2005 (has links)
No description available.
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Identification and characterization of a novel transcription factor that regulates NCF2 expression via the TNF-alpha responsive regionAnderson, Mary Cloud Bosworth Ammons. January 2007 (has links) (PDF)
Thesis (Ph. D.)--Montana State University--Bozeman, 2007. / Typescript. Chairperson, Graduate Committee: Mark T. Quinn. Includes bibliographical references (leaves 147-162).
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Etude de la famille génétique des NAD(P)H déshydrogénases de type II chez lalgue verte unicellulaire Chlamydomonas reinhardtii et étude de la fonction dune déshydrogénase chloroplastique.Jans, Frédéric 20 September 2010 (has links)
Les NAD(P)H déshydrogénases de type II (Ndh-II) sont des enzymes de faible poids moléculaire capables doxyder le NAD(P)H et de transférer les électrons à un groupement quinone (plastoquinone ou ubiquinone). On les appelle « de type II » par opposition aux déshydrogénases de type I qui correspondent au complexe I mitochondrial. Chez Arabidopsis thaliana, des protéines Ndh-II ont été identifiées sur les faces interne et externe de la membrane interne mitochondriale, sur la membrane des peroxysomes, et au niveau de la membrane thylacoïdale du chloroplaste.
Au niveau de la chaîne de transport délectrons mitochondriale, les protéines Ndh-II constituent une voie alternative aux complexes I et II pour lapport des électrons au pool dubiquinones. Cette voie alternative permettrait une adaptation de la chaîne de transport délectrons en fonction du métabolisme de lalgue. Au niveau de la chaîne de transport délectrons chloroplastique, les protéines Ndh-II participeraient à plusieurs mécanismes dadaptation de la chaîne à la quantité et à la qualité de la lumière disponible : transitions détats, transport cyclique délectrons autour du photosystème II. Leur fonction serait de catalyser la réduction non-photochimique du pool de plastoquinones.
En 2005, sept open reading frame correspondant à des NAD(P)H déshydrogénases de type II hypothétiques (NDA1 à NDA7) ont été identifiées dans le génome nucléaire de Chlamydomonas. Ces séquences étaient cependant largement incomplètes du fait de régions non séquencées dans le génome de Chlamydomonas. Les données récoltées au cours de ce travail ont permis lobtention dune version complète de la séquence codante des gènes NDA de Chlamydomonas. Ces analyses ont démontré que le gène putatif NDA4 correspondait, en fait, à des régions internes non attribuées au gène NDA2.
Chez Arabidopsis thaliana et Solanum tuberosum, une corrélation entre le positionnement phylogénétique des gènes NDH-II et la localisation subcellulaire de la protéine correspondante a été mise en évidence. Lanalyse phylogénétique des séquences des protéines Nda de Chlamydomonas montre que les gènes NDA1, 2 et 3 seraient proches phylogénétiquement et seraient à positionner dans le clade des protéines Ndh-II mitochondriales des plantes supérieures. A linverse, la protéine Nda5 serait dorigine cyanobactérienne et se positionne dans le même clade que les protéines identifiées dans le chloroplaste des plantes supérieures. Les protéines Nda6 et 7 sont très proches du point de vue de la séquence, suggérant une duplication récente des gènes NDA6 et 7. Ces deux protéines se positionnent dans un nouveau clade, apparemment intermédiaire entre le domaine eucaryote et le domaine procaryote.
Une étude dexpression des gènes NDA de Chlamydomonas a permis de mettre en évidence lexpression apparemment majoritaire du gène NDA2. Pour étudier la fonction spécifique de NDA2, nous avons inactivé lexpression de ce gène par RNA interférence afin détudier le phénotype des mutants obtenus. Contrairement aux prédictions in silico, il est apparu que la protéine Nda2 se localise au niveau du chloroplaste. Létude de la fluorescence chlorophyllienne de deux mutants montre que la capacité de ces mutants à réduire de manière non-photochimique le pool de plastoquinones est largement diminuée. Dautre part, les mutants sont largement affectés dans leur capacité à modifier la distribution de lénergie dexcitation entre les deux photosystèmes (transition détat) lorsque la respiration mitochondriale est inhibée.
Il est connu que les transitions détat sont initiées par des changements de létat rédox du pool de plastoquinones, qui est lui-même dépendant de létat rédox de la cellule. Dans ce cadre, nous proposons que la protéine Nda2 pourrait servir de « senseur » du métabolisme cellulaire de lalgue et permettrait dadapter les flux délectrons chloroplastiques en réponse aux changements du contexte énergétique cellulaire.
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The mechanisms of NADH oxidation and electron transfer in NADH:ubiquinone oxidoreductaseYakovlev, Gregory January 2007 (has links)
No description available.
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NADPH oxidase in PC12 cell differentiation and apoptosisBegdache, Lina. January 2008 (has links)
Thesis (Ph. D.)--State University of New York at Binghamton, Department of Biological Sciences, 2008.
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Contribuição do complexo NAD(P)H oxidase na disfunção de aorta torácica de camundongos jovens tratados com colesterol / Contribution of NAD(P)H oxidase complex in dysfunction of the thoracic aorta of young mice treated with cholesterolMoreira, Rafael Pires 04 April 2014 (has links)
Vários fatores são responsáveis pelo desenvolvimento de doenças cardiovasculares, dentre eles o alto consumo de colesterol. Segundo a AHA, o consumo recomendado de colesterol é de 300mg/dia. No entanto esses valores nem sempre são respeitados. Na maioria das vezes a consequência do alto consumo de colesterol é o desenvolvimento da dislipidemia, a qual favorece o desenvolvimento da disfunção endotelial. É descrito que o alto consumo de colesterol pode levar ao risco do desenvolvimento de doenças cardiovasculares antes mesmo do desenvolvimento da dislipidemia, uma vez que, em alguns casos o consumo recomendado de colesterol na dieta diária é ultrapassado, e mesmo em estágios crônicos desse consumo não se observa o seu desenvolvimento. Dessa forma o consumo crônico de altas concentrações de colesterol poderia favorecer alterações no sistema vascular, antes mesmo da instalação do processo dislipidêmico. O objetivo desse trabalho foi estudar os efeitos da ingestão de altas concentrações de colesterol por camundongos sobre as repostas de angiotensina II (Ang II) em aorta torácica, bem como avaliar a participação da NAD(P)H oxidase nesse processo. Os animais foram divididos em grupo tratados com ração colesterol 1% (RC) ou ração colesterol padrão (RP), durante 1 mês ou 3 meses. O tratamento com RC não alterou o peso dos animais nem foram observadas alterações morfológicas em aorta torácica, sugerindo que a alteração na funcionalidade deste tecido não foi decorrente de modificações estruturais. O tratamento com RC acarretou no aumento dos níveis basais de O-2 produzido em parte pela NOX-1 e NOX-4, além do aumento da expressão proteica de NOX-1 em aorta torácica de animais tratados 3 meses. No estudo funcional foi observado um aumento do Emax da Ang II em animais tratados durante 1 mês e 3 meses, uma reposta independente de endotélio. Esse aumento foi restaurado na presença de Tiron, mostrando a contribuição de O-2 no aumento do Emax de Ang II em aorta torácica. Na presença de inibidores de NOX-1 e NOX-4 também foi observada à restauração do Emax observada na ausência dos mesmos, mostrando a participação das subunidades na produção de O-2. Os animais tratados com RC não apresentaram alterações no perfil lipídico, esse fato deve-se principalmente pela falta do ácido cólico na dieta. O Emax da Ang II foi aumentado em aorta torácica de camundongos tratados com RC em decorrência de aumento nos níveis basais de O-2 produzido pela NOX-1 e NOX-4. Os níveis basais de O-2 aumentados possivelmente devem esta reagindo com o NO diminuindo sua disponibilidade, aumentando assim os efeitos contrateis da Ang II em aorta torácica. Algumas hipóteses são levantadas para explicar o aumento basal de O-2 em aorta torácica. 1º Aumento na concentração de colesterol nos bolsões lipídicos presente nas caveolas. 2º Formação de oxisterois, 3º formação de micropartículas. Os resultados obtidos e as hipóteses levantadas nesse trabalho, como, aumento na produção de oxisterois e micropartículas podem servir como possíveis alvos de estudos para o desenvolvimento de novos marcadores, e no diagnostico precoce de alterações vasculares decorrentes do consumo excessivo de colesterol iniciada em indivíduos jovens. / Several factors are responsible for the development of cardiovascular disease, including high cholesterol intake. According to AMH, the recommended intake of cholesterol is 300 mg/ daily, however these values are not always respected. Most often the consequence of high cholesterol consumption is the development of dyslipidemia, which favors the development of endothelial dysfunction. It is described that a high intake of cholesterol can lead to the risk of developing cardiovascular disease even before the development of dyslipidemia, since in some cases the recommended daily intake of cholesterol in the diet is exceeded, and even chronic stages of consumption not observing the development. Thus, chronic exposure to high concentrations of cholesterol could promote changes in the vascular system, even before the installation process dyslipidemic. The aim of this work was to study the effects of ingestion of high concentrations of cholesterol in mice on the responses to angiotensin II (Ang II) in the thoracic aorta, as well as evaluate the involvement of NAD(P)H oxidase in this process. The animals were divided into groups treated with 1% cholesterol diet (CD) or standard chow (SC) for 1 month or 3 months. Treatment with SC did not change the weight of the animals was not observed morphological changes in the thoracic aorta, suggesting that the change in the functionality of this tissue was not due to structural changes. Treatment with SC resulted in increased basal levels of O-2, produced in part by NOX- 1 and NOX- 4, in addition to increased protein expression of NOX- 1 in the thoracic aorta of animals treated three months . In the functional study, we observed an increase in the Emax of Ang II in animals treated for 1 month and 3 months, an independent response of endothelium. This increase was restored in the presence of Tiron, showing the contribution of O-2 in increasing the Emax of Ang II in the thoracic aorta. In the presence of inhibitors of NOX-1 and NOX-4 was also observed Emax restoration observed in the absence thereof, showing the involvement of subunits for the production of O-2. The animals treated with CD showed no changes in lipid profile, this is due largely to the lack of cholic acid in the diet. The Emax of Ang II was increased in the thoracic aorta of mice treated with CD as a result of an increase in basal levels of O-2, produced by NOX- 1 and NOX- 4. The basal levels of O-2 increased this should possibly reacting with NO decreasing its availability, thereby increasing the contractile effects of Ang II in thoracic aorta. Some hypotheses were proposed to explain the increased basal O-2 in the thoracic aorta. 1º Increase in the concentration of cholesterol in lipid pockets present in caveolae. 2º Formation of oxysterols; 3º microparticle formation . The results obtained and the hypotheses in this work, as an increase in the production of oxysterols and microparticles can serve as potential targets for the development of studies of new markers, and early diagnosis of vascular alterations caused by excessive consumption of cholesterol began in young individuals.
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Miogeninių kamieninių ląstelių morfologinių ir biocheminių pokyčių diferenciacijos metu tyrimas / Investigation of morphologic and biochemical change in myogenic stem cells during differentiationJakštaitė, Aldona 20 June 2012 (has links)
Suaugusio organizmo miogeninės kamieninės ląstelės yra potencialus šaltinis širdies raumens audinio regeneravimui po išemijų. Dėka šių kamieninių ląstelių daugelis raumeninių organizmo audinių turi galimybę regeneruoti.
Tam, kad šias ląsteles būtų galima naudoti gydymo tikslais, reikia patvirtinti ląstelių diferenciaciją į raumenines ląsteles. Tam naudojami brangūs, daug laiko atimantys imunofluorescenciniai metodai, kurie yra invaziniai ir ardantys ląsteles, tačiau tikslingiausia būtų naudoti neinvazinius ir ląstelių neardančius metodus.
Yra žinoma, kad diferenciacijos metu kinta daugelis ląstelės struktūrų. Šio darbo tikslas buvo įvertinti triušio miogeninių Fr3 linijos kamieninių ląstelių morfologinius ir biocheminius pokyčius, atsirandančius diferenciacijos metu. Todėl šiame darbe buvo tiriamas Fr3 kamieninių ir tos pačios linijos diferencijuotų ląstelių mitochondrijų išsidėstymas ląstelėse, įvertinti branduolių ir bendro baltymų kiekio, mikroskopinio vaizdo dydžio, formos bei morfologiniai pokyčiai, išmatuota NAD(P)H būdingoji ir nuo NADH priklausoma fluorescencija diferenciacijos eigoje.
Atlikus tyrimus nustatėme, kad kamieninių ląstelių mitochondrijos yra linkusios išsidėstyti šalia branduolio, o diferencijuotose - tolygiai pasiskirstyti citoplazmoje. Taip pat pastebėjome, kad nediferencijuotose ląstelių kultūrose yra 6 % ląstelių, turinčių daugiau nei vieną brandulį, o po diferenciacijos tokių ląstelių aptinkama jau 25%. Ląstelių dydis po 7 dienų diferenciacijos... [toliau žr. visą tekstą] / Myogenic stem cells of adult organism are the potential source of cells for tissues repairing. One of the applications is the cell transplantation for the repair of the injured myocardium after ischemia.
The use of stem cells in the medicine has a high potential but first it must be proved that isolated stem cells differentiate in to the muscle cells. To prove the differentiation, invasive, cell-destroying, time-consuming and expensive imunofluorescence methods are used. The best method would be noninvasive and nondestroying to the cells.
The fact, that cells are changing morphologically and biochemically during differentiation, can be used to establish easier and cheaper methods to assess the differentiation level.
The aim of this study was to evaluate the morphologic and biochemical changes in miogenic Fr3 stem cells during differentiation.
In the present study, myogenic stem cells derived from rabbit femur muscle Fr3 were analyzed. We have studied the changes in mitochondrial distribution, number of multinuclear cells, cell size, shape, protein amount during differentiation. We have also evaluated NAD(P)H and NADH fluorescence in undifferentiated and differentiated stem cells.
We have found that mitochondria in undifferentiated stem cells have perinuclear localization, and mitochondria of differentiated stem cells are even scattered in the cytoplasm. The number of multinuclear cells in undifferentiated stem cells culture was 6 %, and in differentiated cells culture was 25... [to full text]
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