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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
31

The Optimization and Applications of Magic Angle Spinning Surface Micro-Coil Probes in Nuclear Magnetic Resonance

Ke, Wea-len 25 May 2011 (has links)
The most critical problem of NMR spectroscopy and magnetic resonance imaging (MRI) is the relatively low sensitivity compared to other forms of spectroscopy, limiting the applicability of these techniques. Most of the existing research focused on trying to alleviate these problems through hyperpolarization techniques, strong magnetic fields, low temperature experiments (<25K), and pulse sequence development. Going beyond the previously stated methods, researchers found that the implementation of smaller resonant coils is a more convenient and effective way to alleviate the problem of low sensitivity in NMR spectroscopy and magnetic resonance imaging. In this work, a systematic optimization of the parameters for micro-coil probes and magic-angle coil spinning (MACS) probes was carried out with various common NMR nuclear species such as 1H, 31P, 23Na, 79Br on two NMR spectrometers (200 MHz and 500 MHz). The optimized wire diameter, coil diameter, number of turns, the inner and outer diameters of the capillary, the matching capacitors etc have been obtained and demonstrated with a real biological system. In addition, we found that the conventional placement of the sample within the glass tube wrapped by a coil yields a lower signal and sensitivity when compared to coating the sample onto the coil prior to wrapping around the capillary. A new method of performing MACS experiment with micro-coil technology, therefore, was subsequently proposed, namely, micro surface coil magic angle spinning (MISCMAS). The optimized experimental conditions were then determined with both liquid and solid state samples.
32

Design of Mixing Pulses for NMR Spectroscopy by Repeated Rotating Frames

Coote, Paul William 06 June 2014 (has links)
In protein NMR spectroscopy, homonuclear mixing pulses are used to reveal correlations amongst chemically bonded nuclear spins. / Engineering and Applied Sciences
33

Structural and Conformational Studies of Oligo- and Polysaccharides

Zaccheus, Mona January 2012 (has links)
The focus of this thesis is to examine the structural properties of polysaccharides produced by bacteria, as well as the dynamic and conformational behavior of a synthetically derived oligosaccharide. The primary structures of the O-polysaccharide repeating units of four different Escherichia coli (E. coli) strains, namely O175, O177, O103 and TD2158, as well as the first report of a capsular polysaccharide produced by lactic acid bacteria Leuconostoc mesenteroides ssp. cremoris PIA2 are reported in paper I–V. Structural analyses have been performed using a combination of nuclear magnetic resonance spectroscopy and chemical component analysis. The elucidated structures in paper I–III, as well as paper V, are composed of linear repeating units of varying composition and length. In paper IV, the structure of the O-polysaccharide repeating unit of E. coli TD2158 is determined to be a branched hexasaccharide structure with a heterogeneous substitution pattern, with either a β-GlcpNAc or β-Glcp residue branching to the backbone chain. Incubation with bacteriophage HK620 tailspike protein shows that the polysaccharide is selectively cleaved at the α-GlcpNAc-(1→2)-α-Rhap-linkage of the backbone chain, yielding a 9:1 ratio of β-GlcpNAc/β-Glcp containing hexasaccharides after digestion. In paper VI the conformational properties of a trisaccharide, which constitutes an internal epitope of the LeaLex hexasaccharide over-expressed on the surface of squamous lung cancer cells, have been analyzed using NMR spectroscopy and molecular dynamics simulations. The β-(1→3)-linkage of the trisaccharide was shown to be highly flexible. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Submitted. Paper 6: Submitted.</p>
34

Structural and functional characterization of E2A:KIX interactions in leukemia

Denis, Christopher 15 September 2012 (has links)
The E2A proteins are transcription factors critical for B-lymphopoiesis. A chromosomal translocation involving the E2A gene promotes acute lymphoblastic leukemia (ALL) through expression of the oncoprotein E2A-PBX1. Two activation domains of E2A-PBX1, AD1 and AD2, have been implicated in transcription mediated by recruitment of the transcriptional co-activator CBP/p300. A motif has been identified within AD1 that is important for recruiting CBP/p300, known as PCET. This recruitment requires an interaction between the activation domains of E2A-PBX1 and the KIX domain of CBP/p300. The KIX domain recognizes a generic ΦXXΦΦ sequence (Φ corresponds to a hydrophobic residue) found in the activation domains of numerous transcription factors. Mutation of leucine 20 in PCET has been shown to abrogate ex vivo immortalization of murine bone marrow and oncogenesis in a murine bone marrow transplantation model. A similar sequence is also found in AD2 and is implicated in E2A transcriptional activity and recruitment of CBP/p300. The structural details of these interactions remain largely unknown. NMR spectroscopy was used to determine the solution structure of the PCET:KIX complex, and the functional consequences of the Leu20Ala mutation were structurally rationalized. Other PCET mutations informed by this structure were tested and correlations were found between in vitro binding affinities and both transcriptional activation and immortalization. The binding site of the ΦXXΦΦ-containing E2A AD2 peptide was mapped to the same site on the KIX domain used by the PCET motif. A model of this complex was generated and mutations were tested using a similar approach as was used for PCET. E2A AD2 binds the KIX domain with lower affinity than the PCET motif and is not required for immortalizing bone marrow. A mutation that increases the affinity of E2A AD2 for the KIX domain to levels approaching that seen for the PCET:KIX interaction restores transcriptional activation and immortalization, demonstrating that immortalization by E2A-PBX1 is an affinity dependent process involving the KIX domain of CBP/p300. These studies indicate that the activation domains of E2A-PBX1 serve to support the in vivo function of the oncoprotein and that the PCET:KIX complex is a potential target for novel therapeutics in E2A-PBX1+ leukemia. / Thesis (Ph.D, Biochemistry) -- Queen's University, 2012-09-13 13:30:48.848
35

NMR studies of metabolites and xenobiotics: From time-points to long-term metabolic regulation

Ehlers, Ina January 2015 (has links)
Chemical species carry information in two dimensions, in their concentrations and their isotopic signatures. The concentrations of metabolites or synthetic compounds describe the composition of a chemical or biological system, while isotopic signatures describe processes in the system by their reaction pathways, regulation, and responses to external stimuli. Stable isotopes are unique tracers of these processes because their natural abundances are modulated by isotope effects occurring in physical processes as well as in chemical reactions. Nuclear magnetic resonance (NMR) spectroscopy is a prime technique not only for identification and quantification of small molecules in complex systems but also for measuring intramolecular distribution of stable isotopes in metabolites and other small molecules. In this thesis, we use quantitative NMR in three fields: in food science, environmental pollutant tracing, and plant-climate science. The phospholipid (PL) composition of food samples is of high interest because of their nutritional value and technological properties. However, the analysis of PLs is difficult as they constitute only a small fraction of the total lipid contents in foods. Here, we developed a method to identify PLs and determine their composition in food samples, by combining a liquid-liquid extraction approach for enriching PLs, with specialized 31P,1H-COSY NMR experiments to identify and quantify PLs. Wide-spread pollution with synthetic compounds threatens the environment and human health. However, the fate of pollutants in the environment is often poorly understood. Using quantitative deuterium NMR spectroscopy, we showed for the nitrosamine NDMA and the pesticide DDT how intramolecular distributions (isotopomer patterns) of the heavy hydrogen isotope deuterium reveal mechanistic insight into transformation pathways of pollutants and organic compounds in general. Intramolecular isotope distributions can be used to trace a pollutant’s origin, to understand its environmental transformation pathways and to evaluate remediation approaches. The atmospheric CO2 concentration ([CO2]) is currently rising at an unprecedented rate and plant responses to this increase in [CO2] influence the global carbon cycle and will determine future plant productivity. To investigate long-term plant responses, we developed a method to elucidate metabolic fluxes from intramolecular deuterium distributions of metabolites that can be extracted from historic plant material. We show that the intramolecular deuterium distribution of plant glucose depends on growth [CO2] and reflects the magnitude of photorespiration, an important side reaction of photosynthesis. In historic plant samples, we observe that photorespiration decreased in annual crop plants and natural vegetation over the past century, with no observable acclimation, implying that photosynthesis increased. In tree-ring samples from all continents covering the past 60 – 700 years, we detected a significantly smaller decrease in photorespiration than expected. We conclude that the expected “CO2 fertilization” has occurred but was significantly less pronounced in trees, due to opposing effects. The presented applications show that intramolecular isotope distributions not only provide information about the origin and turnover of compounds but also about metabolic regulation. By extracting isotope distributions from archives of plant material, metabolic information can be obtained retrospectively, which allows studies over decades to millennia, timescales that are inaccessible with manipulation experiments.
36

Multidimensional in vivo NMR

Welch, John January 2001 (has links)
A proton nuclear magnetic resonance spectrum of the brain in vivo contains peaks from every proton-containing molecule in the brain. Sensitivity limitations mean that only those molecules present at concentrations of at least a few millimolar are detectable in a reasonable period of time; this still leaves many important molecules such as amino acids and other small metabolites. Most of their resonance frequencies fall in the region between 1.0 and 4.5 p.p.m. A typical linewidth in vivo is about 0.05 p.p.m., so the number of distinct peaks observable is restricted. The use of two-dimensional NMR techniques such as COSY can spread peaks out into a second dimension enabling otherwise overlapping peaks to be resolved. This thesis describes the development, testing and application of two such 2D NMR pulse sequences, dubbed ISIS-COSY and ISIS-JRES. They are based on an existing magnetisation localisation sequence and excite detected magnetisation in a manner analogous to the high-resolution sequences COSY and 2D J-resolved spectroscopy. A method for quantifying the metabolites visible in an ISIS-COSY spectrum from their cross-peak intensities is described, and results presented from both control rat brains and those of animals treated with vigabatrin, an inhibitor of GABA-transaminase that has the effect of increasing brain γ-amino butyric acid (GABA) levels. Further applications mentioned are in the study of neutrophil-infiltrated rat brain and adaptation of the ISIS-COSY technique for human use.
37

Protein Design Based on a PHD Scaffold

Kwan, Ann Hau Yu January 2004 (has links)
The plant homeodomain (PHD) is a protein domain of ~45�100 residues characterised by a Cys4-His-Cys3 zinc-binding motif. When we commenced our study of the PHD in 2000, it was clear that the domain was commonly found in proteins involved in transcription. Sequence alignments indicate that while the cysteines, histidine and a few other key residues are strictly conserved, the rest of the domain varies greatly in terms of both amino acid composition and length. However, no structural information was available on the PHD and little was known about its function. We were therefore interested in determining the structure of a PHD in the hope that this might shed some light on its function and molecular mechanism of action. Our work began with the structure determination of a representative PHD, Mi2b-P2, and this work is presented in Chapter 3. Through comparison of this structure with the two other PHD structures that were determined during the course of our work, it became clear that PHDs adopt a well-defined globular fold with a superimposable core region. In addition, PHDs contain two loop regions (termed L1 and L3) that display increased flexibility and overlay less well between the three PHD structures available. These L1 and L3 regions correspond to variable regions identified earlier in PHD sequence alignments, indicating that L1 and L3 are probably not crucial for the PHD fold, but are instead likely to be responsible for imparting function(s) to the PHD. Indeed, numerous recent functional studies of PHDs from different proteins have since demonstrated their ability in binding a range of other proteins. In order to ascertain whether or not L1 and L3 were in fact dispensable for folding, we made extensive mutations (including both insertions and substitutions) in the loop regions of Mi2b-P2 and showed that the structure was maintained. We then went on to illustrate that a new function could be imparted to Mi2b-P2 by inserting a five-residue CtBP-binding motif into the L1 region and showed this chimera could fold and bind CtBP. Having established that the PHD could adopt a new binding function, we next sought to use combinatorial methods to introduce other novel functions into the PHD scaffold. Phage display was selected for this purpose, because it is a well-established technique and has been used successfully to engineer zinc-binding domains by other researchers. However, in order to establish this technique in our laboratory, we first chose a control system in which two partner proteins were already known to interact in vitro. We chose the protein complex formed between the transcriptional regulators LMO2 and ldb1 as a test case. We have examined this interaction in detail in our laboratory, and determined its three-dimensional structure. Furthermore, inappropriate formation of this complex is implicated in the onset of T-cell acute lymphoblastic leukemia. We therefore sought to use phage display to engineer ldb1 mimics that could potentially compete against wild-type ldb1 for LMO2, and this work is described in Chapter 4. Using a phage library containing ~3 x 10 7 variants of the LMO2-binding region of ldb1, we isolated mutants that were able to interact with LMO2 with higher affinity and specificity than wild-type ldb1. These ldb1 mutants represent a first step towards finding potential therapeutics for treating LMO-associated diseases. Having established phage display in our laboratory, we went on to search for PHD mutants that could bind selected target proteins. This work is described in Chapter 5. We created three PHD libraries with eight randomized residues in each of L1, L3 or in both loops of the PHD. These PHD libraries were then screened against four target proteins. After four rounds of selection, we were able to isolate a PHD mutant (dubbed L13-FH6) that could bind our test protein Fli-ets. This result demonstrates that a novel function can be imparted to the PHD using combinatorial methods and opens the way for further work in applying the PHD scaffold to other protein design work. In summary, the work detailed in Chapters 3 and 5 demonstrates that the PHD possesses many of the properties that are desirable for a protein scaffold for molecular recognition, including small size, stability, and a well-characterised structure. Moreover, the PHD motif possesses two loops (L1 and L3) of substantial size that can be remodeled for target binding. This may lead to an enhancement of binding affinities and specificities over other small scaffolds that have only one variable loop. In light of the fact that PHDs are mainly found in nuclear proteins, it is reasonable to expect that engineered PHDs could be expressed and function in an intracellular environment, unlike many other scaffolds that can only function in an oxidizing environment. Therefore, our results together with other currently available genomic and functional information indicate PHD is an excellent candidate for a scaffold that could be used to modify cellular processes. Appendices 1 and 2 describe completed bodies of work on unrelated projects that I have carried out during the course of my PhD candidature. The first comprises the invention and application of DNA sequences that contain all N-base sequences in the minimum possible length. This work is presented as a reprint of our recently published paper in Nucleic Acids Research. The second Appendix describes our structural analysis of an antifreeze protein from the shorthorn sculpin, a fish that lives in the Arctic and Antarctic oceans. This work is presented as a manuscript that is currently under review at the Journal of the American Chemical Society.
38

Localized Proton Magnetic Resonance Spectroscopy of Mouse Brain In Vivo at High Magnetic Field Strength

Abaei Tafresh, Alireza 13 May 2013 (has links)
No description available.
39

¹⁷O Solid-state NMR spectroscopy of functional oxides for energy conversion

Halat, David Michael January 2018 (has links)
The main aim of this thesis is the development of $^{17}$O solid-state nuclear magnetic resonance (NMR) spectroscopic techniques to study the local structure and ion dynamics of functional oxide materials for applications in energy conversion, in particular as electrodes and electrolytes in solid oxide fuel cells (SOFCs). Broadly, the work comprises two related areas: (1) application of a combined experimental and computational methodology to enable the first $^{17}$O solid-state NMR studies of paramagnetic oxides, in particular a class of perovskite-derived structures used as mixed ionic-electronic conductors (MIECs) for SOFC cathodes, and (2) further uses of multinuclear variable-temperature NMR spectroscopy, with emphasis on $^{17}$O NMR results, to elucidate mechanistic details of oxide-ion motion and sublattice exchange in a novel family of promising SOFC electrolyte materials based on $\delta$-Bi$_{2}$O$_{3}$. In the first section, $^{17}$O magic-angle spinning (MAS) NMR spectra of the paramagnetic MIEC, La$_{2}$NiO$_{4+\delta}$, are presented and rationalized with the aid of periodic DFT calculations. Advanced NMR pulse programming and quadrupolar filtering techniques are coupled to extract high-resolution spectra. In particular, these data reveal local structural distortions in La$_{2}$NiO$_{4+\delta}$ that arise from incorporation of interstitial oxide defects. Moreover, variable-temperature spectra indicate the onset of oxide-ion motion involving the interstitials at 130 °C, which is linked to an orthorhombic$-$tetragonal phase transition. By analyzing the ion dynamics on the spectral timescale, specific motional mechanisms are elucidated that prove relevant to understanding the functionality and conductivity of this phase. Next, a similar methodology is applied to the Sr-doped analogues, La$_{2-x}$Sr$_{x}$NiO$_{4+\delta}$, in an exploration of the defect chemistry and electronic structure of these phases (0 $\leq {x} \leq$ 1). By following the doping-induced evolution of spectral features assigned to interstitial and equatorial oxygen environments, changes in the ionic and electronic conductivity, respectively, are rationalized. This approach has been extended to the acquisition and assignment of $^{17}$O NMR spectra of isostructural Sm$_{2-x}$Sr$_{x}$NiO$_{4+\delta}$ and Pr$_{2-x}$Sr$_{x}$NiO$_{4+\delta}$ phases, promising SOFC cathode materials that exhibit paramagnetism on the A site (A = Sm, Pr). The final section details the characterization of oxide-ion motion in the fluorite-type phases Bi$_{1-x}$V$_{x}$O$_{1.5+x}$ and Bi$_{1-x}$P$_{x}$O$_{1.5+x}$ ($x$ = 0.087 and 0.148) developed as SOFC electrolytes. Variable-temperature NMR experiments between room temperature and 923 K reveal two distinct mechanisms. For the V-doped phases, an oxide-ion conduction mechanism is observed that involves oxygen exchange between the Bi-O sublattice and rapidly rotating VO$_{4}$ tetrahedral units. The more poorly conducting P-doped phase exhibits only vacancy conduction with no evidence of sublattice exchange, a result ascribed to the differing propensities of the dopants to undergo variable oxygen coordination. These initial insights suggest chemical design rules to improve the next generation of oxide-ion conducting materials.
40

Analysis of secondary metabolites in plant and cell culture tissue of <em>Hypericum perforatum</em> L and <em>Rhodiola rosea</em> L.

Tolonen, A. (Ari) 22 November 2003 (has links)
Abstract Sensitive chromatographic methods were developed for the quantitative analysis of secondary metabolites in Hypericum perforatum (St. John's wort) and Rhodiola rosea (Golden root, rose root) extracts. Sample preparation methods were developed for plant, cell culture and biotransformation suspension matrixes. High performance liquid chromatography (HPLC) was used for the separation of analytes, and chromatographic data was acquired using photodiode array (PDA) detection or atmospheric pressure ionization mass spectrometry (API-MS). Ionization efficiencies with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were compared under different conditions. Specific mass spectrometric detection methods such as multiple reaction monitoring (MRM) and selective ion monitoring (SIM) were utilized. For identification of known and new secondary metabolites in plant tissues, mass spectrometric methods with triple quadrupole and time-of-flight mass spectrometers were used together with one- and two-dimensional nuclear magnetic resonance spectroscopy (NMR).

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