Woodrats (Neotoma spp.) are imperiled in the north-central and north eastern United States. In Illinois, eastern woodrats (N. floridana) experienced range reductions and population bottlenecks over the past century. Hypothesized reasons for the decline of many woodrat populations that inhabit rock outcrops in the eastern United States include parasitism by raccoon roundworms (Baylisascaris procyonis), hard mast shortages, owl predation, and reductions in crevice availability for nest construction. During 2004-2005, the isolated remnant populations along the Mississippi bluffs in southwestern Illinois were genetically augmented with 47 eastern woodrats from Arkansas and Missouri resulting in 40% admixture within the largest population. In 2009, a strong windstorm created canopy gaps and woody debris throughout this area, potentially improving habitat for eastern woodrats. During 2003–2009, 422 eastern woodrats were reintroduced to 5 sites in the southeastern Illinois, and 172 eastern woodrats to 2 southern Illinois state parks during 2013–2014. These reintroductions are the only woodrat reintroductions to date with >50 individuals released per site. Most previous woodrat reintroduction attempts have released small numbers of individuals (10–15 per site and 10–54 total) and either failed to establish populations or required frequent management for populations to persist. My objectives were to (1) investigate the status of augmented eastern woodrat populations in southwestern Illinois, (2) evaluate the success of the southern Illinois reintroductions, (3) investigate whether eastern woodrats demographics within a reintroduced metapopulation could be predicted by factors underlying hypothesized reasons for woodrat declines, and (4) develop and evaluate noninvasive alternatives to live-trapping and sign surveys for monitoring woodrat populations.

Key Largo woodrats are an endangered subspecies with an extremely limited habitat. This study sought to understand woodrat habitat preferences in order to guide management. Woodrats build stick nests from natural and artificial materials, so nest distribution and nest occupancy were used as indicators of preference. Distribution was determined by nest surveys, and remote cameras were used to assess occupancy. Forest structure, human disturbance, nest, and animal presence metrics were also collected. More nests were found along abandoned roads than along forest transects and more artificial nests were occupied than natural nests. These findings indicate that woodrats prefer areas with human disturbance, rather than forest age and structure as previously believed. This may have consequences on woodrat management, as it suggests that man-made materials are currently needed by woodrats even in a protected natural area.