Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-25T23:14:34Z No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-04T20:58:54Z (GMT) No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) / Made available in DSpace on 2016-08-04T20:58:54Z (GMT). No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) Previous issue date: 2016-02-18 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Os nevos melanoc?ticos (NMs) s?o prolifera??es benignas de c?lulas n?vicas, que podem serencontradas na pele e em mucosas de revestimento, incluindo a mucosa oral. Contudo, osNMs cut?neos s?o mais comuns, quando comparados os que acometem a mucosa oral. Osmecanismos moleculares envolvidos no desenvolvimento dos nevos e os fatores que podeminfluenciar no padr?o de migra??o das c?lulas n?vicas s?o pouco explorados. O objetivo destapesquisa foi analisar a express?o imuno-histoqu?mica das prote?nas E-caderina e Bcl-2 emNMs orais/cut?neos e relacion?-las com as caracter?sticas cl?nicas (sexo, idade, localiza??o,exposi??o ? radia??o solar) e tipos histopatol?gicos. Foram analisados 36 casos de NMs orais34 de NMs cut?neos. Foi utilizada a t?cnina de imuno-histoqu?mica das prote?nas E-caderinae bcl-2, na qual foram analisados a intensidade (fraca, intermedi?ria e forte) e distribui??o demarca??o (focal e difusa). A imunoexpress?o tamb?m foi analisada quanto aos tipos de c?lulasn?vicas (A, B e C). A an?lise estat?stica foi realizada atrav?s dos testes de Qui-Quadradode Pearson e Correla??o de Spearman com n?vel de signific?ncia estabelecido em 5%. Dos 70casos de NMs, 82,9% eram do sexo feminino, 48,6% com idade entre 26-50 anos, 60% eramda ra?a branca, 51,4% foram diagnosticados histopatologicamente como nevos intrad?rmicos/intramucosos e 80% eram NMs adquiridos. A express?o imuno-histoqu?mica da bcl2 e Ecaderinaforam vari?veis na amostra e n?o exibiram associa??o com os par?metros cl?nicos. Aexpress?o da bcl-2 e E-caderina foram vari?veis de acordo com os tipos de c?lulas n?vicas (A,B e C) (p=0,001). A express?o da bcl-2 foi mais difusa em NMs cong?nitos (p=0,002). A Ecaderinafoi positiva em 83,3% dos NMs <1cm (p=0,001) e em exibiu uma fraca marca??oem 73,9% dos NMs que se encontravam em ?reas expostas (p=0,010). Com base nestes resultados,sugere-se que a E-caderina tenha um controle na determina??o dos tipos histopatol?gicosdos NMs, e que a bcl-2 seja um poss?vel marcador de NMs com maior susceptibilidade aodesenvolvimento de les?es malignas. / Melanocytic nevi (MNs) are benign melanocytic proliferations of cells, which can be found in the skin and mucous coat, including the oral mucosa. However, skin NMs are more common when compared to those that affect the oral mucosa. The molecular mechanisms involved in the development of nevi and the factors that can influence the migration pattern of the nevus cells are little explored. The aim of this study was to analyze the immunohistochemical expression of E-cadherin protein and Bcl-2 in oral / skin NMs and relate them to the clinical characteristics (gender, age, location, exposure to solar radiation) and histopathological types. 36 cases of oral NMs and 34 Skin NMs were analyzed. The immunohistochemistry was used of the protein E-cadherin and bcl-2, which were analyzed the intensity (weak, moderate and strong) and distribution marking (diffuse and focal). The immunoreactivity also analyzed as to the types of nevus cells (epithelioid cells -A, -B lymphocyte and fibroblast-like -C). Statistical analysis was performed using the chi-square tests of Pearson and Spearman correlation with significance level set at 5%. Of the 70 cases of NMs, 82.9% were female, 48.6% aged 26-50 years, 51.4% were diagnosed histologically as intradermal / intramucosal nevi and 80% were NMs acquired. Immunohistochemical expression of BCL2 and E-cadherin were variables in the sample and showed no association with clinical parameters. The expression of bcl-2 and E-cadherin were variable according to the types of nevus cells (A, B and C) (P = 0.001). The expression of bcl-2 was more diffuse in congenital MNs (p = 0.002). E-cadherin was positive in 83.3% of MNs <1cm (p = 0.001) and exhibited weak staining in 73.9% of MNs that were in exposed areas (p = 0.010). Based on these results, it is suggested that the E-cadherin has a modulating effect on the migratory properties of NMs, and bcl-2 is a marker of MNs with increased proliferative capacity.