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xiv, 181 leaves : photos. (part col.) tables, graphs ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agronomy, 1979

Thesis (Ph. D.)--University of Adelaide, Dept. of Agr. Biochemistry, Waite Agr. Research Inst., 1984. / Includes bibliographical references (leaves 183-202).

Thesis (Ph.D.)-- University of Adelaide, Dept. of Agricultural Biochemisty, 1979.

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The effects of starch and phosphorus (P) source on P partitioning and ruminal phytase activity were evaluated in eight lactating cows (113 DIM). Four cows were ruminaly cannulated. Cows were randomly assigned to treatments in a duplicated 4x4 Latin square with four, 18-d periods. Diets included dry ground corn (DG) or steam flaked corn (SF), with a no supplemental P (low P diet; 0.34% P) or supplemental purified phytic acid (PA; 0.45% P) to provide additional P from an organic source. Total collection of milk, urine, feces, and feed were sampled each period, while rumen fluid was sampled on d 18. Excretion of feces, urine, P, and N was lower in cows fed SF than in cows fed DG. Milk yield was unaffected by diet despite a lower DMI by cows fed SF. Cows fed SF tended to have a higher feed efficiency and lower milk urea nitrogen (MUN) concentration than cows fed DG. Rumen pH was unaffected by diet, but milk fat content was lower for cows fed SF. Milk yield, DMI, and feed efficiency were not affected by PA. Cows fed PA had increased P intake and excretion, but a lower milk P as a percentage of intake compared with cows fed the low P diet. An interaction of starch source and P source was observed for ruminal phytase activity. Altering dietary sources of starch and P offers opportunity to improve P availability and reduce manure nutrient excretion. / Master of Science

<p>Successful management of nitrogen (N) excretion is important for sustainable pork production. Two experiments were conducted and their objectives were to study the effect of dietary protein and fibre on N excretion patterns and to relate plasma urea (PU) to urinary N excretion.</p> <p>In the first experiment, three dietary protein contents (high, 197; medium, 169; low, 138 g kg^-1 ) and two levels of fibre (low and high) were tested. Diets (wheat, barley, soybean meal; oat-hulls as the fibre source) were formulated to an equal digestible energy (DE) content (3.25 Meal DE kg^-1) and at least 2.18 g digestible lysine per Meal DE, and were supplemented with Lys, Met, Trp, Thr, Ile, or Val. Pigs (32 ± 3.4 kg; n = 42) were housed in confinement-type metabolism crates for 19 d. On d 10 or 11, catheters were installed by cranial vena cava venipuncture. Daily feeding rates were adjusted to three times maintenance and daily rations were halved to two equal meals. Faeces and urine were collected from d 15 to 19. Five blood samples were collected in two-h intervals on d 16 and 19. Faecal N, urinary N, and total N excretion were reduced linearly with a reduction of dietary protein content (P < 0.001). Reduction was greater for urinary (48%) and total N excretion (40%) than for faecal N (23%) excretion. The ratio of urinary N to faecal N was reduced linearly with a reduction of dietary protein content (P < 0.001). Retention of N (g d^-1) was reduced linearly but N retention as percentage of intake was increased linearly with a reduction of dietary protein content (P < 0.001). Addition of oat hulls did not affect N excretion patterns (P > 0.10). Dietary treatments did not affect average daily gain (ADG) or feed efficiency (P > 0.1 0). Plasma urea increased after feeding, peaked at 4 h and then decreased toward pre-feeding concentrations. A linear relationship of urinary N to PU concentration (R² = 0.66) was observed.</p> <p>In the second experiment, effects of dietary protein content (high, 185; and low, 157 g kg^-1) and fibre sources on N excretion patterns were studied in a 2x3 factorial arrangement. The three fibre sources were control, soybean hulls (SH; 15% ), and sugar beet pulp (SBP; 20%). Diets were formulated to 3.3 Meal DE kg^-1 and 2.4 g digestible Lys per Meal DE, and were supplemented with Lys, Met, Trp, Thr, lie, or Val. Pigs (30.5 ± 3 kg; n = 36) were housed in confinement-type metabolism crates, with restricted access to feed (3 x maintenance DE) from d 1 to 18, and free access to feed from d 19 to 26. Faeces and urine were collected from d 15 to 18 and d 23 to 26, and blood samples on d 17 and 25. With restricted access to feed, faecal N (as % of N intake) was increased 3 percentage units for low compared to high protein content, and increased 4 percentage units for SH and 6.5 percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high protein content, and reduced 9 percentage units for SBP compared to control (P < 0.05). Retention of N (g d^-1) was reduced 12% for low compared to high protein content (17.9 compared to 20.4 g d^-1 ; P < 0.05), and was similar among fibre treatments (P > 0.10). With free access to feed, faecal N (as % of N intake) was increased 2.5 percentage units for low compared to high protein content, and increased 5 percentage units for SH and 9 percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high protein content, and reduced 9 percentage units for SH and 10 percentage units for SBP compared to control (P < 0.05). Retention of N (g d^-1) was similar for dietary protein content (30.5 g d^-1 ; P > 0.10), and reduced for SH (27 g d^-1; P < 0.05) compared to control (31 g d^-1), while was intermediate for SBP (29 g d^-1). A linear relationship of urinary N to PU concentration was observed with both restricted (R² = 0.66) and free access to feed (R²= 0.71).</p> <p>Reduction of dietary protein content is an efficient way to reduce total and urinary N excretion, which may reduce ammonia emission. Inclusion of fibre sources high in fermentable fibre shifted N excretion from urine N to faeces N while fibre sources resistant to fermentation did not have any effects on N excretion patterns. Combined effects of dietary protein content and fibre reduced urinary N excretion further than single effects. Level of feed intake is an important consideration when effectiveness of a nutrient management strategy is studied. Diets with a low protein content and containing synthetic AA should be studied using pigs with free access to feed to verify that N retention is maintained. A linear relationship of urinary N to PU concentration was observed under both restricted and free access to feed indicating that urinary N excretion could be predicted from PU concentration.</p>

<p>Successful management of nitrogen (N) excretion is important for sustainable pork production. Two experiments were conducted and their objectives were to study the effect of dietary protein and fibre on N excretion patterns and to relate plasma urea (PU) to urinary N excretion.</p> <p>In the first experiment, three dietary protein contents (high, 197; medium, 169; low, 138 g kg^-1 ) and two levels of fibre (low and high) were tested. Diets (wheat, barley, soybean meal; oat-hulls as the fibre source) were formulated to an equal digestible energy (DE) content (3.25 Meal DE kg^-1) and at least 2.18 g digestible lysine per Meal DE, and were supplemented with Lys, Met, Trp, Thr, Ile, or Val. Pigs (32 ± 3.4 kg; n = 42) were housed in confinement-type metabolism crates for 19 d. On d 10 or 11, catheters were installed by cranial vena cava venipuncture. Daily feeding rates were adjusted to three times maintenance and daily rations were halved to two equal meals. Faeces and urine were collected from d 15 to 19. Five blood samples were collected in two-h intervals on d 16 and 19. Faecal N, urinary N, and total N excretion were reduced linearly with a reduction of dietary protein content (P < 0.001). Reduction was greater for urinary (48%) and total N excretion (40%) than for faecal N (23%) excretion. The ratio of urinary N to faecal N was reduced linearly with a reduction of dietary protein content (P < 0.001). Retention of N (g d^-1) was reduced linearly but N retention as percentage of intake was increased linearly with a reduction of dietary protein content (P < 0.001). Addition of oat hulls did not affect N excretion patterns (P > 0.10). Dietary treatments did not affect average daily gain (ADG) or feed efficiency (P > 0.1 0). Plasma urea increased after feeding, peaked at 4 h and then decreased toward pre-feeding concentrations. A linear relationship of urinary N to PU concentration (R² = 0.66) was observed.</p> <p>In the second experiment, effects of dietary protein content (high, 185; and low, 157 g kg^-1) and fibre sources on N excretion patterns were studied in a 2x3 factorial arrangement. The three fibre sources were control, soybean hulls (SH; 15% ), and sugar beet pulp (SBP; 20%). Diets were formulated to 3.3 Meal DE kg^-1 and 2.4 g digestible Lys per Meal DE, and were supplemented with Lys, Met, Trp, Thr, lie, or Val. Pigs (30.5 ± 3 kg; n = 36) were housed in confinement-type metabolism crates, with restricted access to feed (3 x maintenance DE) from d 1 to 18, and free access to feed from d 19 to 26. Faeces and urine were collected from d 15 to 18 and d 23 to 26, and blood samples on d 17 and 25. With restricted access to feed, faecal N (as % of N intake) was increased 3 percentage units for low compared to high protein content, and increased 4 percentage units for SH and 6.5 percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high protein content, and reduced 9 percentage units for SBP compared to control (P < 0.05). Retention of N (g d^-1) was reduced 12% for low compared to high protein content (17.9 compared to 20.4 g d^-1 ; P < 0.05), and was similar among fibre treatments (P > 0.10). With free access to feed, faecal N (as % of N intake) was increased 2.5 percentage units for low compared to high protein content, and increased 5 percentage units for SH and 9 percentage units for SBP compared to control (P < 0.05). Urinary N was reduced 5 percentage units for low compared to high protein content, and reduced 9 percentage units for SH and 10 percentage units for SBP compared to control (P < 0.05). Retention of N (g d^-1) was similar for dietary protein content (30.5 g d^-1 ; P > 0.10), and reduced for SH (27 g d^-1; P < 0.05) compared to control (31 g d^-1), while was intermediate for SBP (29 g d^-1). A linear relationship of urinary N to PU concentration was observed with both restricted (R² = 0.66) and free access to feed (R²= 0.71).</p> <p>Reduction of dietary protein content is an efficient way to reduce total and urinary N excretion, which may reduce ammonia emission. Inclusion of fibre sources high in fermentable fibre shifted N excretion from urine N to faeces N while fibre sources resistant to fermentation did not have any effects on N excretion patterns. Combined effects of dietary protein content and fibre reduced urinary N excretion further than single effects. Level of feed intake is an important consideration when effectiveness of a nutrient management strategy is studied. Diets with a low protein content and containing synthetic AA should be studied using pigs with free access to feed to verify that N retention is maintained. A linear relationship of urinary N to PU concentration was observed under both restricted and free access to feed indicating that urinary N excretion could be predicted from PU concentration.</p>

Bibliography: leaves 183-202 / xxiv, 202 leaves, [46] leaves of plates : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agricultural Biochemistry, Waite Agricultural Research Institute, 1984