Regensburg, Univ., Diss., 2009.

Applied research project (D. Min.)--School of Theology and Missions, Oral Roberts University, 2004. / Includes abstract and vita. Includes bibliographical references (leaves 164-170).

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 157-169).

<p>ABSTRAKT</p><p>Det här är en sammanfattning av den formella delen av vårt projektarbete. Under hösten 2007 och våren 2008 har vi på uppdrag av Egon Nilsson överhalat en hjälpmaskin ombord på Calmare Nyckel. Maskinen var i stort behov av en renovering, det läckte både kylvatten och smörjolja på olika ställen. Det har varit mycket väntetid på reservdelar eftersom maskinen är ovanlig och tillverkas i England. Mot slutet av arbetet råkade vi på bekymmer med vattenläckage i smörjoljan som gjorde att jobbet blev försenat några veckor. Läckaget lagades och maskinen provkördes. Resultatet blev bra, maskinen fungerade och gick som den skulle. Under arbetets gång har vi försökt i möjligaste mån följa de instruktioner som finns i instruktionsboken.</p><p>När man genomför ett projektarbete av den här typen, skall all dokumentation rörande projektet redovisas. Materialet som presenteras i den här sammanställningen är upplagt enligt följande. Inledning till ämnet och projektet vilket följs av avsnitten projektprocessen och resultat. Till denna dokumentation har vi bifogat, arbetsdagbok och offert.</p> / <p>ABSTRACT</p><p>This is a summary of the formal part of our project. During the autumn of 2007 and spring 2008 we have done an overhaul on one of the auxiliary engines onboard Calmare Nyckel. The assignment was given from Egon Nilson. The engine was in bad shape, lubrication oil and coolingwater was leaking from several cylinders and thereby in big need of a service. There have been some waiting time during the order of new parts because of the rare engine type which are British and therefore all the parts had to be ordered from England. At the end of the project we discovered some difficulties with coolingwater leaking in to the oil sump, this causing further delays finishing the project. The problem were solved and at the test run the engine run satisfactory.</p><p>During the project we have as far as possible followed the manual and other instructions available.</p><p>During a project like this it is important that all documentation of the project is shown.</p><p>The materials that are shown are presented with an introduction followed by the process and the result. To this documentation we have attached a working diary and quotations.</p>

No Abstract Provided

Defect control in colloidal crystals is essential for these nanostructures to be effective as photonic bandgap (PBG) materials. We have used in-situ monitoring of the PBG of a colloidal crystal to study the structural changes during colloidal self assembly, with a focus on the formation of macroscopic defects such as cracks. These findings allow us to model the final stages of colloidal self assembly and explain the formation of growth defects in colloidal crystal. Our model suggests that cracks are intrinsic to self assembly growth methods. . However, by tuning the interaction potential between the colloids, it is possible to minimize the cracks in colloidal crystals. / Singapore-MIT Alliance (SMA)

Thesis (Ph. D.)--University of Chicago, Dept. of Physics, March 2001. / Includes bibliographical references. Also available on the Internet.

Resumo: A acirrada competitividade entre as empresas de manufatura, presente no cenário atual, exige a busca por sistemas produtivos cada vez mais eficientes e que estejam inseridos num ambiente de constante aprimoramento. Visando à redução de estoques, e agilidade na produção, o conceito de produção enxuta se apresenta como um conjunto de importantes ferramentas operacionais. Desde que surgiram os conceitos associados à produção enxuta, vários estudos que tratam da utilização eficaz de Linhas de Montagem de Modelos Mistos - LMMM se concentram no sequenciamento de tais linhas. Neste trabalho, linhas de montagem múltiplas para modelos mistos, em ambiente de produção enxuta, são estruturas operacionais nas quais vários fornecedores internos abastecem várias linhas de montagem de modelos mistos simultaneamente, de modo que todas as linhas possam receber peças ou subconjuntos de todos os fornecedores. Para otimizar este sistema, a sequência de programação deve buscar o consumo constante de peças ou subconjuntos, minimizando, assim, o dimensionamento dos kanbans e estoques intermediários e, ainda, nivelar a carga de trabalho em cada posto, minimizando as paradas de linha. Baseado no clássico Problema de Monden, que determina o sequenciamento de uma única linha, este trabalho desenvolve um modelo computacional utilizando a Meta-heurística Simulated Annealing e a Simulação a Eventos Discretos para o sequenciamento do sistema de abastecimento cruzado com várias linhas. O modelo proposto busca o sequenciamento das linhas de montagem que mais aproxima o consumo real de um consumo ideal constante e atende a um padrão de eficiência predefinido para as linhas / Abstract: The fierce competition between manufacturing companies, in this current scenario requires the search for production systems more efficient, and which are embedded in an environment of continuous improvement. Seeking to reduce inventories, and speed of production, the concept of lean production is presented as a range of important operational tools. Since the rise of concepts associated with lean production, several studies dealing with the effective use of assembly lines mixed models - LMMM focus on the sequencing of such lines. In this work, multiple assembly lines for mixed models, in an environment of lean production, are operating structures where several domestic suppliers supply many assembly lines mixed models simultaneously, so that all lines can get parts or subsets of all suppliers. To optimize this system, the programming sequence must seek constant consumption of parts or subassemblies, thus minimizing the scaling of kanbans, and intermediate stocks and also level the workload at each station to minimize line stoppages. Based on the classic problem Monden, which determines the sequencing of a single line, this paper develops a computational model using the Simulated Annealing metaheuristic and Discrete Event Simulation for the sequencing of the supply system with crossed several lines. The proposed model seeks the sequencing of assembly lines that most approximates the actual consumption of a consumption ideal constant and respects a predefined standard of efficiency for the lines / Orientador: Fernando Augusto Silva Marins / Coorientador: Jose Arnaldo Barra Montevechi / Banca: Antonio Augusto Chaves / Banca: Jorge Muniz Júnior / Banca: Dagoberto Alves de Almeida / Banca: Rodrigo Scarpel / Doutor

miRNAs are a species of small-regulatory RNA that post-transcriptionally regulate gene expression via the RNA induced silencing complex (RISC). They are encoded ubiquitously among animals and plants, and have recently been shown to be encoded by the majority of herpesviruses. It seems likely that herpesvirus encoded miRNAs have evolved as a tool for the manipulation of host-cellular and viral-gene expression during infection. Human cytomegalovirus (HCMV) is a clinically important herpesvirus that represents a significant cause of morbidity and mortality in the immune-compromised. HCMV encodes as many as 25 miRNAs during infection, but the function of the majority of these is not known. Identifying the targets of HCMV miRNAs will not only establish a basis for understanding the role of miRNAs within the context of HCMV infection, but also provide a means for discovering novel host-virus interactions. Using RISC immunoprecipitation and siRNA screening, host-cellular targets of viral miRNAs that play important roles in the biology of HCMV were identified. ATP6VOC, a key component of the vacuolar-ATPase, was shown to be a target of miR-US25-1 and subsequent siRNA knockdown of ATP6VOC resulted in the almost complete inhibition of infectious virion production. Despite this, ATP6VOC knock-down did not inhibit viral entry, DNA synthesis, or gene expression, highlighting a possible role for ATP6VOC in the assembly and egress of HCMV. A critical step in HCMV assembly and egress is the formation of the juxta-nuclear virion assembly compartment (VAC). The HCMV VAC is derived from host-cellular endocytic and secretory vacuoles, and is crucial for the efficient nuclear egress of nucleocapsids, cyotplasmic tegumentation, final envelopment, and the egress of mature virions. Using siRNA knock-down, immunofluorescence-microscopy, and western-blot analysis, a crucial role for ATP6VOC and v-ATPase function in the formation of the VAC was demonstrated. siRNA knock-down of ATP6VOC resulted in a failure in the reorganisation of trans-golgi and early-endosomal compartments during infection, resulting in a failure in VAC formation. These findings demonstrate a crucial role for ATP6VOC during infection, and in so doing identify a novel host factor that is required for HCMV assembly.

abstract: Coronaviruses are medically important viruses that cause respiratory and enteric infections in humans and animals. The recent emergence through interspecies transmission of severe acute respiratory syndrome coronavirus (SARS-CoV) strongly supports the need for development of vaccines and antiviral reagents. Understanding the molecular details of virus assembly is an attractive target for development of such therapeutics. Coronavirus membrane (M) proteins constitute the bulk of the viral envelope and play key roles in assembly, through M-M, M-spike (S) and M-nucleocapsid (N) interactions. M proteins have three transmembrane domains, flanked by a short amino-terminal domain and a long carboxy-terminal tail located outside and inside the virions, respectively. Two domains are apparent in the long tail - a conserved region (CD) at the amino end and a hydrophilic, charged carboxy-terminus (HD). We hypothesized that both domains play functionally important roles during assembly. A series of changes were introduced in the domains and the functional impacts were studied in the context of the virus and during virus-like particle (VLP) assembly. Positive charges in the CD gave rise to viruses with neutral residue replacements that exhibited a wild-type phenotype. Expression of the mutant proteins showed that neutral, but not positive, charges formed VLPs and coexpression with N increased output. Alanine substitutions resulted in viruses with crippled phenotypes and proteins that failed to assemble VLPs or to be rescued into the envelope. These viruses had partially compensating changes in M. Changes in the HD identified a cluster of three key positive charges. Viruses could not be recovered with negatively charged amino acid substitutions at two of the positions. While viruses were recovered with a negative charge substitution at one of the positions, these exhibited a severely crippled phenotype. Crippled mutants displayed a reduction in infectivity. Results overall provide new insight into the importance of the M tail in virus assembly. The CD is involved in fundamental M-M interactions required for envelope formation. These interactions appear to be stabilized through interactions with the N protein. Positive charges in the HD also play an important role in assembly of infectious particles. / Dissertation/Thesis / Ph.D. Microbiology 2010