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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Aspects of growth and metabolism in the musculature of Antarctic fishes, with particular reference to Notothenia neglecta Nybelin

Fitch, Neil Antony January 1986 (has links)
1. Aspects of the physiology and biochemistry of Antarctic fish muscle have been examined, principally for the nototheniid, Notothenia neglecta Nybelin. 2. Sampling was most successful during the late summer/autumn. The trammel net was the best method for catching fish above 200mm standard length. Smaller fish were best caught by SCUBA divers using hand-nets. 3. The condition factor for fish caught was highest during the summer (high food availability) and remained low during the winter months. Analysis of trunk muscle constituents (water, protein, lipid) indicated no evidence for marked depletion at any time of the year. Seasonal variation in relative gonad size and relative liver size were observed. 4. Metabolically the pectoral abductor muscle of N.neglecta had higher activities of anaerobic and aerobic enzymes than either the red or white trunk muscles. This is in line with the fact that this species normally swims using enlarged pectoral fins, while sprint swimming in short bursts is carried out by the white trunk muscle. 5. Differences in the metabolic profiles of the trunk muscles of 3 Antarctic species (N.neglecta, Notothenia gibberifrons and Trematomus newnesi) are related to their different lifestyles, particularily feeding behaviours. 6. Muscle growth in N.neglecta is probably both by hypertrophy, and recruitment of myosatellite cells. Both the fibres and capillaries in Antarctic species are much larger in cross-sectional area than for temperate species. 7. Lactate dehydrogenase isozymes in the trunk and cardiac muscles of N.neqlecta are electrophoretically and kinetically similar. This, along with its high activities of anaerobic enzymes indicates that the cardiac muscle is adapted to utilise fuels anaerobically if necessary. 8. The majority of physiological and cellular adaptations can be explained on the basis of physiological/ecological constraints rather than cold temperature per se.
2

Molecular systematics and antifreeze biology of sub-Antarctic notothenioid fishes

Miya, Tshoanelo Portia January 2014 (has links)
Fishes of the perciform suborder Notothenioidei are found in Antarctic and sub-Antarctic waters that are separated by the Antarctic Polar Front (APF), with some species being distributed on both sides of this front. In this wide latitudinal range, these fishes are exposed to different temperatures ranging from -2 °C in the High Antarctic regions to 12 °C in the sub-Antarctic regions. To survive in icy Antarctic waters, the Antarctic notothenioid species have evolved antifreeze glycoproteins (AFGPs) that prevent their body fluids from freezing. The findings of past research on the AFGP attributes of several notothenioid species inhabiting ice-free sub-Antarctic environments have presented a complex picture. Furthermore, previous taxonomic studies split widely distributed notothenioids into different species and/or subspecies, with other studies disagreeing with these splits. To understand the response of the sub-Antarctic notothenioids to warmer, ice-free environments, it is necessary to have a good understanding of their antifreeze biology and systematics. Therefore, this study aimed to determine the association, if any, between the antifreeze attributes of sub-Antarctic notothenioid fishes and their taxonomic status. And more...
3

Histoire évolutive des remaniements chromosomiques en liaison avec la mobilisation d'éléments transposables chez les téléostéens antarctiques Nototheniidae : la radiation adaptative du groupe " Trematomus " / Evolutionary history of chromosomal rearrangements linked with the mobilization of transposable elements within the Antarctic teleosts Nototheniidae : the adaptive radiation of the group “Trematomus”

Auvinet, Juliette 19 October 2018 (has links)
L’alternance de périodes glaciaires et interglaciaires durant les 20 derniers Ma a mené à des changements environnementaux répétés au niveau du plateau continental antarctique. C’est dans ce contexte que les téléostéens de la famille des Nototheniidae se sont adaptés et diversifiés à travers plusieurs vagues de radiations (dont les Trematominae), dominant l’Ichtyofaune australe. Parmi les Nototheniidae, le groupe « Trematomus » (genres Cryothenia, Pagothenia, Trematomus et Indonotothenia) est celui où l’on observe la plus grande diversité chromosomique, avec des nombres diploïdes de chromosomes allant de 24 à 58, impliquant de nombreux réarrangements ayant accompagné les spéciations. Nous avons cherché à caractériser ces remaniements chromosomiques. Avec un caryotype ancestral inféré de 2n = 48, une conservation des unités chromosomiques entre espèces, et une constance des tailles de génome, l’hypothèse de réarrangements structuraux sans polyploïdisation préalable est la plus probable. Afin de reconstruire l’histoire évolutive de ces événements, nous avons recherché les homologies chromosomiques interspécifiques. Ceci nous a permis de reconstituer les remaniements (majoritairement des fusions) que nous avons repositionnés sur la phylogénie résolue des « Trematomus ». Contrairement à ce qui a été publié pour le genre Notothenia, nos résultats suggèrent des acquisitions multiples et indépendantes. Les éléments transposables (ETs) peuvent être impliqués dans les remaniements chromosomiques par le biais de recombinaisons ectopiques. Ils participent alors à la diversification des lignées au cours de l’évolution. En raison de leur régulation épigénétique, leur mobilisation massive peut être induite en cas de variations environnementales importantes. Nous nous sommes intéressés à trois super-familles d’ETs (DIRS, Gypsy and Copia) dans ces génomes. Les DIRS1 ont montré des patrons d’insertions en points chauds dans les régions centromériques et péricentromériques. Etant donné leur mode de transposition décrit et leur propension à s’insérer dans des copies préexistantes, nous proposons un rôle des éléments DIRS1 comme facilitateurs des fusions observées lors de la diversification des « Trematomus ». / In the last 20 My, multiple glacial-interglacial cycles led to strong and repeated environmental changes on the Antarctic continental shelf. In this changing environment, nototheniid fishes diversified through several rounds of species radiation (one of which within Trematominae), and now constitute the dominant group in Antarctic teleosts. Among Nototheniidae, the group « Trematomus » (genera Cryothenia, Pagothenia, Trematomus and Indonotothenia) exhibits the highest chromosomal diversity, with diploid chromosome numbers ranging between 24 and 58, involving many rearrangements probably linked to speciation. We characterized the nature of these chromosomal repatternings. With an inferred ancestral state of 2n = 48 acrocentric chromosomes, a conserved number of chromosomal structural units, and a constancy of the genomes sizes we measured; the hypothesis of structural modifications is favored rather than a whole genome duplication associated to drastic reductions. In order to reconstruct an evolutionary scenario of such chromosomal rearrangements accompanying the trematomine diversification, we identified interspecific chromosomal homologies. This allowed us to reconstruct the rearrangements events (mostly centric and tandem fusions). We plotted them on a phylogeny we reconstructed based on our own ddRAD-seq data. Contrary to what was reported for the Notothenia, our results are in favor of independent acquisitions. Transposable elements (TEs) can lead to chromosomal rearrangements through ectopic recombination events, hinting at a role as drivers of specific-lineage diversification. Moreover, due to their epigenetic regulation, TEs can be mobilized when thermic changes occur. We focused on three retrotransposon superfamilies (DIRS, Gypsy and Copia) in nototheniid genomes. The DIRS1 showed unexpected accumulation patterns of insertion in the centromeric and pericentromeric regions. Given the mechanism of DIRS1 transposition and their tendency to sometimes insert on pre-existing copies (homing), we suggest a role of DIRS1 elements as facilitators of the fusions that occurred during the trematomine radiation.

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