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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Bridging cell growth and proliferation : identification and characterization of binding partners for pescadillo, a novel nucleolar protein involved in tumorigenesis and DNA damage /

Ho, Joseph Tsung-yo. January 2004 (has links)
Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 105-122).
2

An investigation into the ability of Xbra, a Brachyury homologue in Xenopus laevis, to induce neural tissue

Hartman, Deborah Jane January 2000 (has links)
No description available.
3

Molecular and functional characterization of a novel ring-H2 finger protein, ANAPC11, the anaphase-promoting complex subunit 11. / CUHK electronic theses & dissertations collection

January 2003 (has links)
by Chan Hei. / "July 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 235-247). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.
4

Identification of protein interactors of dribble: a single KH-domain nucleolar protein in Drosophila.

January 2007 (has links)
Choi, Ching Gee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 127-132). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ABSTRACT (Chinese version) --- p.iii / ACKNOWLEDGEMENTS --- p.iv / LISTS OF ABBREVIATIONS --- p.vi / LISTS OF TABLES --- p.ix / LISTS OF FIGURES --- p.x / Chapter 1. --- INTRODUCTION / Chapter 1.1 --- The nucleolus ´ؤ a major site for ribosome biogenesis --- p.1 / Chapter 1.1.1 --- Ribosome biogenesis --- p.1 / Chapter 1.1.1.1 --- Eukaryotic ribosomal RNA processing --- p.4 / Chapter 1.1.1.2 --- Ribosome assembly --- p.5 / Chapter 1.1.2 --- Trans-acting proteins in ribosome biogenesis --- p.8 / Chapter 1.1.3 --- rRNA processing and ribosome assembly are two coordinated process --- p.9 / Chapter 1.2 --- Dribble (DBE) ´ؤ an essential nucleolar RNA-binding protein --- p.11 / Chapter 1.2.1 --- DBE carries a RNA-binding KH-domain --- p.12 / Chapter 1.2.2 --- RNA-binding properties of DBE --- p.13 / Chapter 1.2.3 --- DBE is involved in rRNA processing --- p.15 / Chapter 1.2.4 --- Yeast homolog of DBE 一 Krrlp is associated with ribosome biogenesis --- p.19 / Chapter 1.3 --- Aims of Research --- p.22 / Chapter 2. --- MATERIALS AND METHODS / Chapter 2.1 --- Expression and purification of DBE protein in Escherichia coli --- p.24 / Chapter 2.1.1 --- DNA construct --- p.24 / Chapter 2.1.2 --- Bacterial culture --- p.24 / Chapter 2.1.3 --- Purification of DBE protein --- p.25 / Chapter 2.2 --- Drosophila cell culture and preparation of protein lysates --- p.27 / Chapter 2.2.1 --- Drosophila S2 cell culture --- p.27 / Chapter 2.2.2 --- Preparation of protein lysates from Drosophila cultured cells --- p.27 / Chapter 2.2.3 --- Ribonuclease A (RNase A) treatment of protein lysates from Drosophila cultured cells --- p.28 / Chapter 2.3 --- Drosophila culture and genetics --- p.28 / Chapter 2.3.1 --- Drosophila culture --- p.28 / Chapter 2.3.2 --- GAL4/UAS transgene expression in Drosophila --- p.29 / Chapter 2.4 --- Affinity pull-down --- p.29 / Chapter 2.4.1 --- Immobilization of proteins onto AminoLink Plus coupling beads --- p.29 / Chapter 2.4.2 --- Affinity pull-down using protein coupled-beads --- p.32 / Chapter 2.5 --- Trichloroacetic acid protein precipitation --- p.33 / Chapter 2.6 --- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) --- p.33 / Chapter 2.7 --- Coomassie blue staining and destaining --- p.35 / Chapter 2.8 --- Protein identification by tandem mass spectrometry --- p.35 / Chapter 2.8.1 --- In-gel trypsin digestion of protein bands --- p.35 / Chapter 2.8.2 --- Peptide extraction --- p.37 / Chapter 2.8.3 --- Desalting of digested peptides --- p.38 / Chapter 2.8.4 --- Mass spectrometric analysis of protein candidates --- p.38 / Chapter 2.9 --- Electrotransfer and Western blotting --- p.39 / Chapter 2.10 --- Sucrose gradient sedimentation --- p.41 / Chapter 2.11 --- Glutathione S-transferase (GST) pull-down assay --- p.42 / Chapter 2.11.1 --- Construction of pRSETA-GST-r̐ư̐ưأ̐ơة plasmid --- p.42 / Chapter 2.11.1.1 --- Total RNA preparation from fly heads --- p.42 / Chapter 2.11.1.2 --- DNase treatment on extracted RNA --- p.43 / Chapter 2.11.1.3 --- Reverse Transcription --- p.44 / Chapter 2.11.1.4 --- PCR amplification of DNA fragment for cloning --- p.45 / Chapter 2.11.1.5 --- Agarose gel electrophoresis --- p.47 / Chapter 2.11.2 --- Overexpression of GST and GST-RpS9 proteins --- p.47 / Chapter 2.11.3 --- GST pull-down assay --- p.48 / Chapter 2.12 --- Immunoprecipitation ofFLAG-DBE in Drosophila --- p.49 / Chapter 2.13 --- Reagents and buffers --- p.50 / Chapter 2.13.1 --- Bacterial culture medium --- p.50 / Chapter 2.13.2 --- Buffers for purification of DBE protein in Escherichia coli --- p.51 / Chapter 2.13.3 --- Reagent for preparing protein lysates from Drosophila cultured cells --- p.53 / Chapter 2.13.4 --- Reagents for Drosophila culture and genetics --- p.53 / Chapter 2.13.5 --- Buffers for immobilization of proteins onto AminoLink Plus coupling gel --- p.55 / Chapter 2.13.6 --- Buffers for affinity pull-down using protein coupled-beads --- p.56 / Chapter 2.13.7 --- Reagents for SDS-PAGE --- p.56 / Chapter 2.13.8 --- Reagent for Coomassie Blue Staining and Destaining --- p.58 / Chapter 2.13.9 --- Reagents for protein identification by tandem mass spectrometry --- p.59 / Chapter 2.13.10 --- Reagents for electrotransfer and Western blotting --- p.62 / Chapter 2.13.11 --- Reagents for sucrose gradient sedimentation --- p.63 / Chapter 2.13.12 --- Reagents for agarose gel electrophoresis --- p.64 / Chapter 2.13.13 --- Reagents for immunoprecipitation --- p.65 / Chapter 2.13.14 --- Other common buffer --- p.66 / Chapter 3. --- RESULTS / Chapter 3.1 --- Identification of DBE protein interactors by affinity pull-down --- p.67 / Chapter 3.1.1 --- Introduction --- p.67 / Chapter 3.1.2 --- Results --- p.68 / Chapter 3.1.2.1 --- Expression and purification of DBE protein in E. coli --- p.68 / Chapter 3.1.2.2 --- Affinity pull-down using DBE-coupled beads and the control using lysozyme-coupled beads --- p.70 / Chapter 3.1.2.3 --- Affinity pull-down using DBE-coupled beads and the control using heat-denatured DBE-coupled beads --- p.73 / Chapter 3.1.2.4 --- Tandem mass spectrometric identification of proteins pulled down by DBE-coupled beads --- p.75 / Chapter 3.1.2.5 --- Study of the RNA-dependence of the interactions between DBE and its interactors --- p.83 / Chapter 3.1.3 --- Discussion --- p.89 / Chapter 3.1.3.1 --- Most DBE-interactors pulled down by DBE-coupled beads were shown to be specific --- p.89 / Chapter 3.1.3.2 --- Most of the potential DBE-interactors were found in the nucleolar proteome --- p.91 / Chapter 3.1.3.3 --- Comparison of protein interactions identified in DBE and Krrlp --- p.92 / Chapter 3.1.3.4 --- The implications of DBE-interacting ribosomal proteins on the role of DBE in ribosome biogenesis --- p.94 / Chapter 3.2 --- Study of the sedimentation behavior of DBE by sucrose gradient sedimentation --- p.97 / Chapter 3.2.1 --- Introduction --- p.97 / Chapter 3.2.2 --- Results --- p.98 / Chapter 3.2.2.1 --- The sedimentation behaviors of endogenous DBE and overexpressed FLAG-DBE --- p.98 / Chapter 3.2.3 --- Discussion --- p.100 / Chapter 3.2.3.1 --- The sedimentation behaviors of endogenous DBE and overexpressed FLAG-DBE was similar --- p.100 / Chapter 3.2.3.2 --- DBE associates with a macromolecular complex --- p.101 / Chapter 3.3 --- Study of the interaction between DBE and RpS9 by GST pull-down assay --- p.103 / Chapter 3.3.1 --- Introduction --- p.103 / Chapter 3.3.2 --- Results --- p.104 / Chapter 3.3.2.1 --- The constructs for GST and GST-RpS9 expression --- p.104 / Chapter 3.3.2.2 --- Purified DBE protein was pulled down by GST-RpS9 --- p.104 / Chapter 3.3.3 --- Discussion --- p.107 / Chapter 3.3.3.1 --- Further investigations on the interaction between DBE and RpS9 --- p.107 / Chapter 3.3.3.2 --- The implications of interaction between DBE and RpS9 on ribosome biogenesis --- p.107 / Chapter 3.3.3.3 --- The RNA dependence of the interaction between RpS9 and DBE --- p.111 / Chapter 3.4 --- Study of the association between DBE and histone proteins by co-immunoprecipitation and sucrose gradient sedimentation --- p.112 / Chapter 3.4.1 --- Introduction --- p.112 / Chapter 3.4.2 --- Result --- p.113 / Chapter 3.4.2.1 --- Histone H3 was not co-immunoprecipitated with FLAG-DBE --- p.113 / Chapter 3.4.2.2 --- Histone H3 did not co-sediment with DBE in sucrose gradient sedimentation --- p.115 / Chapter 3.4.3 --- Discussion --- p.116 / Chapter 3.4.3.1 --- Association between DBE and histone H3 could not be found --- p.116 / Chapter 3.4.3.2 --- Further investigation on the association between DBE and histone proteins --- p.118 / Chapter 4. --- GENERAL DISCUSSION --- p.119 / Chapter 5. --- CONCLUSION --- p.125 / Chapter 6. --- REFERENCES --- p.127
5

Tctex-1 : a candidate gene family for a mouse t-complex distorter (tcd-1) locus /

Lader, Eric Scott. January 1989 (has links)
Thesis (Ph. D.)--Cornell University, 1989. / Vita. Includes bibliographical references.
6

Proteins of the cell nucleus

Stevely, W. S. January 1968 (has links)
No description available.
7

Structure and dynamics of the receptor kinase interacting FHA domain of kinase associated protein kinase from arabidopsis /

Lee, Gui-in, January 2003 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 2003. / "August 2003." Typescript. Vita. Includes bibliographical references (leaves 158-174). Also issued on the Internet.
8

Characterisation of the G protein controlled tyrosine kinase, ACK1 and its interaction with nucleolar partner proteins

Krishnan, Kadalmani January 2012 (has links)
No description available.
9

Understanding the role of cofactor of breast cancer susceptinility gene 1 (COBRA1) in transcriptional regulation /

Blair, Ashley Lauren. January 2007 (has links)
Thesis (Ph. D.)--University of Virginia, 2007. / Includes bibliographical references. Also available online through Digital Dissertations.
10

Proteomic analysis of mycobacteria and mammalian cells

Wang, Rong, January 1900 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.

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