x, 160 leaves : ill. ; 26 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Physiology, Waite Agricultural Research Institute, 1971

viii, 181 leaves : ill., appendix / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1972) from the Dept. of Agricultural Biochemistry, University of Adelaide

Thesis (Ph.D.) -- University of Adelaide, Dept. of Plant Physiology, Waite Agricultural Research Institute, 1971.

Thesis (Ph.D. 1972) from the Dept. of Agricultural Biochemistry, University of Adelaide.

Thesis (Ph.D)--University of Adelaide, Dept. of Agricultural Chemistry, 1964. / Typescript. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1980. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1984. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographies.

The synthetic reaction of ribonuclease T_1 has been studied as to its potential for the synthesis of oligoribonucleotides of defined base sequence. Conditions have been determined for maximum synthesis with respect to percentage incorporation of both guanosine 2',3'-cyclic phosphate (donor), and cytidine (acceptor). With regard to maximum acceptor incorporation, a donor blocked at the 5'-hydroxyl has been used to drive the reaction. It has also been shown that acceptor incorporation decreases as the chain-length increases up to the hexanucleotide. Synthesis of a 3'-blocked substrate for polynucleotide phosphorylase for use in chain termination studies was attempted using adenylate kinase. In this regard, adenosine 2'(3'),5'-diphosphate was shown to be a noncompetitive inhibitor with respect to AMP, and a competitive inhibitor with respect to ATP, of adenylate kinase. Polynucleotide phosphorylase was incubated with a protein kinase and a protein phosphatase in an attempt to exploit a postulated phosphorylation- dephosphorylation control mechanism for the production of a primer dependent preparation. No reaction was observed.

No description available.

No description available.