A one-time application of MON1S1S1 or 0.5 lb. ai /a on September 1, 1988, was evaluated for potential overseeding effects. Treated plots, which were overseeded with perennial ryegrass at 10, 20, or 30 lbs. per thousand square feet, were not significantly different compared to untreated plots for percent ryegrass stand and verdure. Only seed rate effects increased percent ryegrass and verdure weights slightly. These data show no detrimental effects of using MON15151 ("Dimension" trade name) six weeks before overseeding.

Anaerobic soil disinfestation (ASD) is a promising alternative to chemical fumigation to control soil-borne plant pathogens and weeds. This research focused on evaluating several locally available carbon sources for ASD on weed control, evaluating the performance of brewers' spent grain (a promising carbon source) under field conditions, and evaluating whether yeast addition enhanced the effectiveness of ASD treatments. A series of greenhouse trials were conducted at the Southern Piedmont AREC (Agricultural Research and Extension Center). The greenhouse trials were conducted in PVC tubes, 20 cm tall and 15 cm in diameter. The first set of trials evaluated ASD conducted over 21-day periods of ASD using locally available carbon sources. The carbon sources included brewer`s spent grain, buckwheat (Fagopyrum esculentum), cowpea (Vigna unguiculata), paper mulch, peanut (Arachis hypogaea) shells, rice bran, sorghum-sudangrass (Sorghum drummondii), and waste coffee grounds applied at 4 mg of C/g of soil. The targeted weed species included common chickweed (Stellaria media (L.) Vill.), redroot pigweed (Amaranthus retroflexus L.), white clover (Trifolium repens L.), and yellow nutsedge (Cyperus esculentus L.). All ASD treatments significantly reduced weed viability compared to the non-treated control. The yeast amendments enhanced weed control over ASD without yeast. The second set of greenhouse trials was focused on ASD using brewer`s spent grain, and on evaluating ASD at the half and one-third carbon dose rates. The target pests were the same weed species in the first set of trials, and Pythium irregulare was added as an additional target pest. This set of trials indicated yeast enhanced addition the effect of BSG in ASD on both weeds and P. irregulare, indicating the potential to reduce carbon input necessary for effective ASD. A follow-up, two seasons, open-field trial conducted over two growing seasons at the Hampton Roads AREC focused on understanding the effects of ASD on weed density and strawberry fruit yield and fruit quality in annual hill strawberry production. The treatments included ASD at standard or half carbon dose rates, with or without yeast. Fumigation (80% chloropicrin + 20% 1,3-dichloropropene) and non-treated plots were used as control groups. Weed suppression with ASD was consistent for most of the broadleaf weed species, and total weed counts were significantly reduced compared to non-treated controls. Yield from ASD with yeast was higher than ASD without yeast and non-treated control in one growing season, while the increase in yield did not occur in another growing season. Yeast may have potentially enhanced the yield effects of ASD but lacked consistency. Yeast may have the potential to enhance ASD effectiveness. / Doctor of Philosophy / Strawberry is a high-value crop known for its brightly colored, sweet tasting, juicy and fleshy fruit that possesses a unique aroma. The southern region is the second large region of strawberry production in the United States. Strawberry is susceptible to soil-borne pests, including weeds and diseases. Preplant control of soil-borne diseases and weeds is important for strawberry production. Early season weeds can compete with newly transplanted strawberry plugs for nutrients, light, and other resources. However, currently, the limited options of pre-plant chemical fumigants and herbicides available in strawberry plasticulture make weed control a challenge in strawberry production. Anaerobic soil disinfestation (ASD) may be an effective alternative to preplant chemical fumigation. Anaerobic soil disinfestation involves three steps- applying carbon sources to the soil, covering the bed with black tarp, and watering the soil to maintain certain soil moisture to field capacity generally for 21 days. However, there are only a few studies on weed control using ASD in the southern region; locally available carbon sources also need to be evaluated. Thus, this study focused on evaluating several locally available carbon sources (cover crops, brewer`s spent grain, used coffee ground, paper mulch, peanut shell) for ASD to control troublesome weeds (common chickweed, redroot pigweed, white clover, yellow nutsedge). This study also explored a new method that involves mixing distiller's yeast with solid carbon sources in order to enhance the ASD weed control effect. Additionally, this study evaluated the effect of ASD using reduced carbon inputs, potentially reducing the total cost of ASD by reducing the carbon input. A series of greenhouse studies were conducted at the Southern Piedmont Agricultural Research and Extension Center (AREC), Blackstone, VA, with a follow-up field study done at the Hampton Roads AREC. The greenhouse trials evaluated carbon sources including brewer`s spent grain, buckwheat, cowpea, paper mulch, peanut shells, rice bran, sorghum-sudangrass, and waste coffee grounds. These greenhouse experiments were conducted in containers made from PVC tubes, and strawberry plants were not involved. The main objective of the greenhouse trial was to test the suppression of four troublesome weeds, including common chickweed, redroot pigweed, yellow nutsedge, and white clover. The most effective treatments in the greenhouse studies were further investigated in the field trial. The brewer`s spent grain was again used in the field trial, and treatments included ASD using a full or half dose of brewer's spent grain, with or without yeast. We evaluated the effects of these treatments on weed control, plant crop growth, and crop yields. Fruit quality factors, including fruit firmness, sweetness, and size, were also evaluated. In summary, all of the carbon sources evaluated provide similar weed control. Adding yeast showed potential to enhance the effect of ASD using brewer`s spent grain. Adding yeast also increased the effectiveness of the half-rate of the carbon source, showing the potential for effective pre-plant pest control for strawberry using ASD treatments with significantly reduced C dose rates.

Cucumber (Cucumis sativus L.), pumpkin (Cucurbita maxima Duch. ex Lam.), and summer squash (Cucurbita pepo L.) are economically important crops in Virginia. Only a few herbicides are registered for weed control in these crops. Halosulfuron is a sulfonylurea herbicide which controls several broadleaf weeds and yellow nutsedge (Cyperus esculentus L.). Cucurbit crops have some tolerance to this herbicide. The efficacy of halosulfuron for control of several weed species and tolerance of four vine crops to halosulfuron were investigated in field and greenhouse studies in 1999, 2000, and 2001. In the field, halosulfuron was applied to cucumber, pumpkin, zucchini squash, and yellow summer squash at 4, 9, 18, and 27 g ai/ha preemergence (PRE) and postemergence (POST) in combination with clomazone at 174 g ai/ha plus ethalfluralin applied PRE at 630 g/ha. Crop injury, weed control, and crop yield was collected from the field studies. Weed control by halosulfuron was dependent upon application method. Halosulfuron applied preemergence controlled only common ragweed (Ambrosia artemisiifolia L.) and smooth pigweed (Amaranthus hybridus L.). Postemergence halosulfuron controlled common ragweed, smooth pigweed, morningglory species (Ipomoea spp.), yellow nutsedge, and rice flatsedge (Cyperus iria L.). All four crops treated with halosulfuron produced yields equal to or higher than the crops receiving clomazone and ethalfluralin alone or the hand-weeded check. In the greenhouse, tolerance of cultivars of each crop to halosulfuron was investigated with the same rates applied in the field. Cultivars responded similar to postemergence halosulfuron applications with respect to fresh and dry weights in all four crops. The response of several populations of acetolactate synthase inhibiting (ALS) resistant smooth pigweed to postemergence halosulfuron was also investigated. Halosulfuron activity against ALS-inhibitor resistant smooth pigweed was population dependent. In the greenhouse, postemergence halosulfuron at the same rates used in the field studies controlled yellow nutsedge. / Master of Science

Cyperus rotundus L. CYPRO (purple nutsedge) and Cyperus esculentus L. CYPES (yellow nutsedge) are problematic weeds on every continent. At present there is no comprehensive means of controling these weeds.. The primary means of control is herbicides, although the weeds are becoming more resistant. Bioherbicide control of purple and yellow nutsedge is an important avenue of research, with much of the focus being to increase the virulence of current fungal pathogens of C. rotundus and C. esculentus. The primary aim of this study was to increase the virulence of a fungal pathogen of C. rotundus and C. esculentus, with the objective of creating a viable bioherbicide. A possible means of increasing the virulence of a pathogen would be to increase the amount of amino acid produced by the fungus. This was proposed as a means of increasing the virulence of Dactylaria higginsii (Luttrell) M. B. Ellis. Overproduction of amino acids such as valine and leucine result in the feedback-inhibition of acetolactate synthase (ALS), an enzyme which is a target for many herbicides currently on the market. By applying various amino acids to tubers of purple nutsedge and comparing the results with a reputable herbicide, glyphosate, it was possible to determine the success of the amino acid applications. Only glutamine treatment at 600 mg.r1 resulted in significantly less (P<O.OOI) germination compared with the water control, while the glyphosate application resulted in no germination. Four treatments were significantly different (P<O.OOI) from the water control in terms of shoot length, but no pattern or conclusion could be drawn from the results. Injecting amino acids and glyphosate into the leaves of the plants gave similar results to those obtained with the tubers, with no visible damage on those plants injected with the amino acids and complete plant death of those injected with glyphosate. Amino acids had little effect on the growth of the C. rotundus plant or tuber. It was later determined by a colleague (Mchunu1 , unpublished) working on the same project, that D. higginsii does not infect the local ecotypes of C. rotundus in Pietermaritzburg, South Africa. A second fungus, Cercospora caricis Oud., was isolated from C. rotundus growing in the region, and confirmed as a Cercospora species by conidial identification. Like many Cercospora species, C. caricis produces a phytotoxin, cercosporin. An increase in production of cercosporin would theoretically lead to an increase in virulence of C. caricis. Mutation of hyphae by i J Makhosi Mchunu: Address: National department ofAgriculture; Private Bag 3917; Port Elizabeth; 6056 Email: Makhosimc@NDA.agric.za ultraviolet-C light was perfected on C. penzigii Sacc., where 5 min exposure to DV-C light resulted in approximately 99% cell death. Surviving colonies were analysed by spectrophoresis, and the surviving mutant gave an absorbance value of approximately 5% more than the median. Samples were analysed by high-performance liquid chromatography (HPLC) to determine the presence of cercosporin. No definitive result was obtained. Exposure of C. caricis to DV-C for 5 min. resulted in approximately 65% hyphal cell death, with 20 min. resulting in approximately 95% death. A spontaneous mutant was observed in a colony that had been exposed to DV-C. This mutant showed sectored growth with red and grey growth patterns. The red section of the mutant was subcultured and analysed by spectrophoresis and HPLC. The red C. caricis gave an absorbance reading of approximately 140 on HPLC compared with about 22 from the grey colony. HPLC analysis of the wild-type C. caricis did not produce a peak corresponding to that of the cercosporin standard, although no conclusion could be obtained on the presence or absence ofthe toxin. The virulence of the mutant C. caricis could not be determined as inoculation experiments were unsuccessful, and had to be discontinued due to time constraints. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2018-03-01T15:11:23Z No. of bitstreams: 1 PGPV17MA226.pdf: 296583 bytes, checksum: 632e5c3fdc904ca285838ae8f37c238d (MD5) / Made available in DSpace on 2018-03-01T15:11:23Z (GMT). No. of bitstreams: 1 PGPV17MA226.pdf: 296583 bytes, checksum: 632e5c3fdc904ca285838ae8f37c238d (MD5) Previous issue date: 2017-02-24 / The Cyperus rotundus L. is a perennial and spontaneous herbaceous plant that infers an area of human interest and interferes detrimentally in agricultural activities. Seeking alternatives for a reduction of competition between cultivated and spontaneous plants that can be applied mainly by agroecological farmers, this work had the objective of evaluating the efficiency of C. rotundus biodynamic ashes and High dynamized dilution on C. rotundus control. The studies were conducted from September 2015 to January 2016, in a greenhouse at the Curitibanos Campus of University Federal of the Santa Catarina. The sample was tray 50.5 x 30 x 8 cm, planted ten tubers, repeated four times. In double blind where there are homeopathies. The experiments with ashes of purple nutsedge differed dates 09/12/15 and 09/26/15: T1-control; T2 -20% acetic acid; T3-ash at 30, 31 and 32 days after plan (DAP); T4-ash at 30, 31 and 32 + 60, 61 and 62 (DAP); T5-ash at 30, 31 and 32 + 60, 61 and 62 + 90, 91 and 92 (DAP); T6 - ashes at 30, 31 and 32 + 60, 61 and 62 + 90, 91 and 92 + 120, 121 and 122 (DAP). The experiment with homeopathies: T1- Magnesia carbonica CH10 (centesimal hahnemanniana), T2- Saccharum officinale CH10, T3-Natrum arsenicum CH10, T4-Limestone CH10, T5-control; 20% T6-acid at 35 and 70 (DAP). The weight of the tubers 4.75 ± 0.15 gr per plot; Seeded in sterilized soil, in a greenhouse with irrigation. The double-blind homeopathic applied at 30, 60, 90 and 120 (DAP). Comments extended around 4.5 months. The results were compared by the student's test and then variance when significant. Treatment with 20% acetic acid seeded (09/26/15) - ash tends to better controls with a mean of 47% up to a maximum of 76% mortality. The acid can collaborate without control, with later researches. / A tiririca roxa (Cyperus rotundus L.) planta herbácea perene prejudicial à agropecuária. Avaliaram-se cinzas dinamizadas de tiririca roxa e preparados homeopáticos no seu controle. A amostra foi bandeja 50,5 X 30 X 8 cm, plantado dez tubérculos, repetida quatro vezes. Em duplo cego onde houver homeopatias. Os experimentos com cinzas de tiririca roxa diferiram datas 12/09/15 e 26/09/15: T1-testemunha; T2 –ácido acético a 20%; T3-cinzas aos 30, 31 e 32 dias após plantio (DAP); T4-cinzas aos 30, 31 e 32 + 60, 61 e 62 (DAP); T5-cinzas aos 30, 31 e 32 + 60, 61 e 62 + 90, 91 e 92 (DAP); T6 – cinzas aos 30, 31 e 32 + 60, 61 e 62 + 90, 91 e 92 + 120, 121 e 122 (DAP). O experimento com homeopatias: T1- Magnesia carbonica na CH10 (centesimal hahnemanniana), T2- Saccharum officinale CH10, T3- Natrum arsenicosum CH10, T4- Calcário dolomítico CH10, T5- testemunha (sem intervenção) e T6 - herbicida alternativo (ácido acético a 20%). O tratamento T6 foi utilizado como testemunha aos 35 e 70 dias após a emergência à aplicação do ácido. Os homeopáticos aplicaram-se aos 30, 60, 90 e 120 (DAP). Em todos os experimentos foi avaliado: a) germinação semanal iniciada um mês após o plantio (MAP), b) altura de plantas, c) diâmetro de cada colmo inicial e dos perfilhos nascidos depois de dois (MAP), d) mortalidade semanal decorrido um (MAP); e) pesagem de fração de 30% da matéria fresca de folhas para o teste de matéria seca; f) pesagem da matéria fresca total aérea; g) pesagem da matéria fresca total das raízes e tubérculos; h) pesagem de fração de 30% da matéria fresca de folhas para o teste de matéria seca de folhas; i) pesagem de fração de 30% da matéria fresca de raízes para o teste de matéria seca de raízes e tubérculos; e j) contagem dos perfilhos totais por tratamento após 30 (DAP). Os dados submetidos à análise de variância e comparados pelo teste t de Student a 5% quando significativo. As cinzas dinamizadas e os preparados homeopáticos avaliados apresentaram efeito discreto a nulo sobre as seguintes variáveis: a, b, c, d, e, f. O ácido acético a 20% promoveu morte de até 76% de plantas de C. rotundus e as médias de mortalidade durante a avaliação foram de 47%, 14,8%, 9,6% para os tratamentos com ácido 17 acético a 20% os experimentos: cinzas 26 09; cinzas 12 09 e homeopatias 12 09 respectivamente. Serão necessários estudos para ajustes de cinzas dinamizados e preparados homeopáticos no controle da tiririca. O preparado a base de ácido acético a 20% pode ser usado com outras técnicas agroecológicas, por apresentar viabilidade

We evaluated the genetic diversity of Penstemon's Centromeric Histone 3 (CENH3), which localizes to chromosome centromeres in eukaryotes. From RNA extractions of 56 species, we successfully amplified two CENH3s (termed CENH3a and CENH3b), which share approximately 69% sequence homology across the length of the gene and about 85% across the histone fold domain (HFD). CENH3a is generally 72 bp longer than CENH3b and contains seven exons while CENH3b is made of five. Unlike studies of CENH3 in other plants, Penstemon's CENH3 N-tail was found to be highly conserved, indicative that the genus has undergone a short evolutionary history. Surprisingly, of the 99 CENH3 sequences obtained during this study, 32 appeared to be mis-spliced and contained premature stop codons. Of those aberrant transcripts, 84.4% originated from CENH3b genomic DNA. Most mis-spliced transcripts resulted from the retention of all or part of an intron. In some cases, all or portions of an exon were missing, including one that was missing the L1 motif. Second, we systematically cataloged interspecific breeding data in Penstemon, on which we then conducted a network analysis. The resulting network provides breeders with a better visualization of successful parental combinations and also identifies gaps in interspecific breeding. This method allowed for the identification of species with a high degree of interspecific compatibility, which we compared to the CENH3 sequencing data. Finally, we studied the performance of soil streaming in high tunnel production of tomatoes (Solanum lycopersicum L.). Our experiments revealed that soil steaming and mulch reduced weed coverage of Palmer amaranth (Amaranthus palmeri S. Watson), large crabgrass (Digitaria sanguinalis L.), and yellow nutsedge (Cyperus esculentus L.). Steam and mulch increased the tomato plant size, fruit size, fruit number, and fruit yield. Additionally, soil steaming reduced tomato southern blight, caused by Sclerotium rolfsii, by 5.8-fold. These findings provide promising results for high tunnel tomato producers, particularly those involved in organic production where pesticide and fumigation use is limited.

Master of Science / Department of Statistics / Leigh Murray / The southern root-knot nematode (SRKN) and the weedy perennials, yellow nutsedge (YNS) and purple nutsedge (PNS) are simultaneously occurring pests in the irrigated agricultural soils of southern New Mexico. Previous research has very well characterized SRKN, YNS and PNS as a mutually-beneficial pest complex and has revealed their enhanced population growth and survival when they occur together. The density of nutsedge in a field could be used as a predictor of SRKN juveniles in the soil. In addition to SRKN, which is the most harmful of the plant parasitic nematodes, in southern New Mexico, other species or categories of nematodes could be identified and counted. Some of them are not as damaging to the plant as SRKN, and some of them may be essential for soil health. The nematode species could be grouped into categories according to trophic level (what nematodes eat) and herbivore feeding behavior (how herbivore nematodes eat). Subsequently, three ratios of counts were calculated for trophic level and for feeding behavior level to investigate the soil nematode community structure. These proportions were modeled as functions of the weed hosts YNS and PNS by generalized linear regression models using the logit link function and three probability distributions: the Binomial, Zero Inflated Binomial (ZIB) and Binomial Hurdle (BH). The latter two were used to account for potential high proportions of zeros in the data. The SAS NLMIXED procedure was used to fit models for each of the six sampling dates (May, July and September) over the two years of the alfalfa study. General results showed that the Binomial pmf generally provided the best fit, indicating lower zero-inflation than expected. Importance of YNS and PNS predictors varied over time and the different ratios. Specific results illustrate the differences in estimated probabilities between Binomial, ZIB and BH distributions as YNS counts increase for two selected ratios.

A cultura da cana-de-açúcar passou por diversas alterações no cenário de manejo de plantas daninhas. Mais recentemente o sistema de plantio de cana-de-açúcar foi diversificado, podendo ser realizado com mudas pré-brotadas (MPB). O manejo de plantas daninhas está em constante mudança, devido à alteração da flora infestante, entretanto, a espécie Cyperus rotundus L. se destaca por aparecer em todos os momentos, devido a dificuldade de controle. Assim, objetivou-se com este estudo: A) Determinar o PAI (Período Anterior à Interferência), o PCPI (Período Crítico de Prevenção à Interferência) e o PTPI (Período Total de Prevenção à Interferência) em cana-de-açúcar plantada por meio de MPB; B) Avaliar o controle químico de plantas daninhas em MPB, com herbicidas utilizados para o manejo de C. rotundus; C) Avaliar o crescimento inicial de MPB após a aplicação de herbicidas utilizados para o manejo de C. rotundus; D) Avaliar o controle e a viabilidade de tubérculos de C. rotundus sob aplicação de herbicidas. Para todos os experimentos foi utilizada a cultivar de cana-de-açúcar IACSP95-5000. Para a determinação dos períodos críticos foi conduzido experimento em campo, no delineamento em blocos ao acaso, com 14 tratamentos e três repetições. Os tratamentos foram compostos por sete períodos de convivência e por sete períodos de controle das plantas daninhas: 0 - 30, 0 - 60, 0 - 90, 0 - 120, 0 - 150, 0 - 210 e 0 - 240 dias após o plantio (DAP). Foram realizadas avaliações de altura, diâmetro, número de perfilhos, peso de dez colmos e produtividade. O experimento de controle foi realizado em campo, no delineamento em blocos ao acaso, com dez tratamentos e quatro repetições. Os tratamentos correspoderam a aplicação de sulfentrazone (800 g i.a. ha-1), diclosulam (193,17 g i.a. ha-1), imazapic (133 g i.a. ha-1) e imazapyr (500 g e.a. ha-1) em pré-plantio (um dia antes do plantio) e, halosulfuron (112,5 g i.a. ha-1), ethoxysulfuron (135 g i.a. ha-1), MSMA (1.975 g i.a. ha-1) e 2,4-D (1.340 g e.a. ha-1) em pós-plantio (30 DAP), além de dois tratamentos controle (com e sem capina). Foram realizadas avaliações de altura, diâmetro, número de perfilhos, controle, sintomas de injúria e produtividade. O experimento de crescimento inicial de MPB foi realizado em casa de vegetação, em delineamento inteiramente casualizado, com nove tratamentos e quatro repetições. Os tratamentos corresponderam aos herbicidas aplicados em pré-plantio: sulfentrazone (200; 400; 800; 1.600 e 3.200 g i.a. ha-1), diclosulam (48,3; 96,6; 193,17; 386,34 e 772,68 g i.a. ha-1), imazapic (33,25; 66,5; 133; 266 e 532 g i.a. ha-1) e imazapyr (125; 250; 500; 1.000 e 2.000 g e.a. ha-1) e, pós-plantio: ethoxysulfuron (33,75; 67,5; 135; 270 e 540 g i.a. ha-1), halosulfuron (28,125; 56,25; 112,5; 225 e 450 g i.a. ha-1), 2,4-D (335; 670; 1.340; 2.680 e 5.360 g e.a. ha-1) e MSMA (493,75; 987,5; 1.975; 3.950 e 7.900 g i.a. ha-1), mais um tratamento controle. Foram avaliados os sintomas de injúria, altura, diâmetro, número de perfilhos, clorofila A, B e total, área foliar, massa seca da parte aérea e de raízes. O experimento de viabilidade de tubérculos foi realizado em casa de vegetação em delineamento inteiramente casualizado, com nove tratamentos e quatro repetições. Os tratamentos foram compostos pelos herbicidas aplicados em pré-emergência: 9 sulfentrazone (800 g i.a. ha-1), diclosulam (193,17 g i.a. ha-1), imazapic (133 g i.a. ha-1) e imazapyr (500 g e.a. ha-1) e em pós-emergência: halosulfuron (112,5 g i.a. ha-1), ethoxysulfuron (135 g i.a. ha-1), MSMA (1.975 g i.a. ha-1) e 2,4-D (1.340 g e.a. ha-1), mais um tratamento controle. Foram avaliados controle e viabilidade de tubérculos. O PAI, PCPI e PTPI foram de 26,26 - 68 e 68 dias, respectivamente. Os herbicidas em pré-plantio proporcionaram os melhores controles até 60 DAP. Entretanto, o diclosulam, imazapic e imazapyr provocaram sintomas de injúria graves reduzindo a produtividade. Os herbicidas de pós-plantio propiciaram sintomas de injúria leves, entretanto no experimento de campo os tratamentos resultaram em queda de produtividade devido ao baixo controle das plantas daninhas. A maior redução na viabilidade de tubérculos foi observada para sulfentrazone, imazapic, halosulfuron, ethoxysulfuron e MSMA. Os herbicidas isolados não foram eficazes para garantir o controle das plantas daninhas até 68 DAP. O correto posicionamento dos herbicidas é necessário para evitar danos severos à cultura. / The sugarcane crop underwent several changes in the weed management scenario. Recently the sugarcane planting system was diversified, being able to be realized with pre-sprouted seedlings (PSS) of sugarcane. Weed management is constantly changing, due to the change in weed flora; however, Cyperus rotundus L. stands out because it appears at all times, due to the difficulty of control. The aim of this study was determine: A) the PPI (Period Prior to Interference), CPIP (Critical Period of Interference Prevention) and TPIP (Total Period of Interference Prevention) in sugarcane in the PSS system; B) Evaluate the chemical control of weeds in PSS with herbicides used for the management of C. rotundus; C) To evaluate the initial growth of PSS of sugarcane after the application of herbicides used for the management of C. rotundus; D) Evaluate the control and viability of C. rotundus tubers under herbicide treatment. For all experiments the sugarcane cultivars used was IACSP95-5000. To determine the critical periods, a field experiment was carried out in a randomized block design with fourteen treatments and three replications. The treatments were composed of seven coexistence periods and seven weed control periods: 0 - 30, 0 - 60, 0 - 90, 0 - 120, 0 - 150, 0 - 210 and 0 - 240 days after planting (DAP). Were evaluated height, diameter, number of tillers, weight of ten stalks and yield. Control experiment was carried out in the field too, in a randomized block design, with ten treatments and four replications. The treatments application corresponding to the herbicides sulfentrazone (800 g a.i. ha-1), diclosulam (193.17 g a.i. ha-1), imazapic (133 g a.i. ha-1) e imazapyr (500 g a.e. ha-1) in pre-planting (um dia antes do plantio) and, halosulfuron (112.5 g a.i. ha-1), ethoxysulfuron (135 g a.i. ha-1), MSMA (1,975 g a.i. ha-1) and 2,4-D (1,340 g a.e. ha-1) in post-planting (30 DAP), and two control treatments (with and without weeding). In the control experiment were evaluated height, diameter, number of tillers, control, symptoms of injury and yield. The experiment of initial growth of PSS was performed in greenhouse, in a completely randomized design, with nine treatments and four replications. The treatments corresponded to the herbicides applied in pre-planting: sulfentrazone (200; 400; 800; 1,600 and 3,200 g a.i. ha-1), diclosulam (48.3; 96.6; 193.17; 386.34 and 772.68 g a.i. ha-1), imazapic (33.25; 66.5; 133; 266 and 532 g a.i. ha-1) and imazapyr (125; 250; 500; 1,000 and 2,000 g a.e. ha-1) and post-planting: ethoxysulfuron (33.75; 67.5; 135; 270 and 540 g a.i. ha-1), halosulfuron (28.125; 56.25; 112.5; 225 and 450 g a.i. ha-1), 2,4-D (335; 670; 1,340; 2,680 and 5,360 g a.e. ha-1) e MSMA (493.75; 987.5; 1,975; 3,950 and 7,900 g a.i. ha-1), plus one control treatment. Were evaluated symptoms of injury, height, diameter, number of tillers, chlorophyll A, B and total, leaf area, shoots and roots dry mass. The C. rotundus tubers viability experiment was carried out in a greenhouse in a completely randomized design, with nine treatments and four replications. The treatments were composed by herbicides applied in pre- emergence: sulfentrazone (800 g a.i. ha-1), diclosulam (193.17 g a.i. ha-1), imazapic (133 g a.i. ha-1) and imazapyr (500 g a.e. ha-1), and post-emergence: halosulfuron (112.5 g a.i. ha-1), ethoxysulfuron (135 g a.i. ha-1), MSMA (1,975 g a.i. ha-1) e 2,4- 11 D (1,340 g a.e. ha-1), plus one control treatment. Were evaluated control and viability of tubers. The results showing that PPI, CPIP and TPIP were 26, 26 - 68 and 68 days, respectively. Pre-planting herbicides provided better controls up to 60 DAP, however, diclosulam, imazapic and imazapyr caused severe injury symptoms reducing yield. Post-planting herbicides were considered selective, however in the field experiment the treatments resulted in a decrease of yield due to low weed control. The lowest viability of tubers was caused by sulfentrazone, imazapic, halosulfuron, ethoxysulfuron and MSMA. The isolated herbicides were not effective to ensure control of weeds up to 68 DAP. The correct herbicides setting is necessary to avoid damage to the crop.

A mudança do sistema de colheita da cana-de-açúcar com o uso de fogo para a colheita mecanizada, chamada de cana verde ou cana crua, é um processo irreversível, gradativo porém contínuo, previsto na legislação estadual paulista. Um dos pontos críticos no processo produtivo da cana-de-açúcar é a interferência imposta pelas plantas daninhas. A tiririca (Cyperus rotundus L.) é uma das plantas daninhas mais prejudiciais à cultura da cana-de-açúcar entretanto, com a implementação da colheita mecanizada, o desenvolvimento desta planta poderá ser afetado. O objetivo do experimento foi verificar se a adição de palha de cana-de-açúcar poderia afetar o desenvolvimento de plantas de tiririca provenientes de tubérculos de tamanho pequeno (0,22 a 0,34 g) e grande (1,01 a 1,14 g), plantados em diferentes épocas do ano. O experimento foi instalado em casa-de-vegetação, em Campinas, SP e disposto em blocos ao acaso com quatro repetições. Tubérculos dormentes de tiririca de tamanho pequeno e grande foram plantados em maio, julho e setembro e adicionou-se sobre o solo palha de cana-de-açúcar RB 855156, nas quantidades correspondentes a 0,0, 5,0, 10,0 e 15,0 t/ha. Em cada época de instalação do experimento, a cada 28 dias até 84 dias, as plantas de tiririca foram avaliadas quanto à altura média, cortadas ao nível do solo. As estruturas subterrâneas e aéreas foram separadas nas diferentes estruturas e avaliou-se suas biomassas frescas e secas e a área foliar. De modo geral, o tamanho maior dos tubérculos favoreceu o desenvolvimento da parte subterrânea. O número de brotações emergidas não foi afetado pelo tamanho de tubérculos mas, a altura média, área foliar e as biomassas das folhas e da parte aérea foram maiores quando originadas de tubérculos de tamanho grande. A adição de palha de cana-de-açúcar ao solo afetou mais as plantas originadas de tubérculos pequenos que de grandes. A adição de quantidades crescentes de palha de cana-de-açúcar causou redução no desenvolvimento nas diferentes estruturas subterrâneas, seguindo para todas as variáveis uma equação do segundo grau. Aplicações de quantidades crescentes de palha de cana-de-açúcar causaram diminuição no número de brotações, área foliar e as biomassas frescas e secas das folhas e da parte aérea total. A formação e o desenvolvimento dos rizomas e de tubérculos + bulbos basais, assim como das biomassas fresca e seca de rizomas e fresca de tubérculos + bulbos basais foram maiores no plantio realizados em setembro e menores nos de julho. Verificou-se que, exceto para altura média, o plantio de setembro proporcionou plantas com maior número de brotações, área foliar e biomassas fresca e seca de folhas e da parte aérea total. Para altura média, os melhores resultados foram observados no plantio de maio. Para todas as variáveis analisadas, o plantio de julho apresentou sempre os menores valores. Quanto ao efeito de época de avaliação, verificou-se que para todas as variáveis analisadas foram observados aumentos lineares em função do tempo. / The change in harvest systems for sugar cane from use of fire to mechanical harvest, is an irreversible process, gradual although continuous, foreseen in the São Paulo State Legislation. One of the critical points in the productive process of sugar cane is the interference imposed by weeds. Purple nutsedge (Cyperus rotundus L.) is one of the most sugar cane crop-damaging weeds, nevertheless with implementation of mechanical harvest technology, which leaves a thick layer straw superior to 20 ton/ha, the development of this plant could be affected. This experiment aimed to verify if the addition of sugar cane straw could affect the development of purple nutsedge plants deriving from tubers of small (0,20-0,34g) and large (1,01-1,14g) sizes, planted at different times of the year. The experiment was carrIED out in glass house, in Campinas, São Paulo, in randomized blocks with four replicates. Tubers of small and large sizes of purple nutsedge were planted in May, July and September, and RB 855156 sugar cane straw was added, in quantities corresponding to 0, 5,0, 10,0 and 15,0 ton/ha. Every 28 days until 84 days after each planting period, the purple nutsedge plants were evaluated as to their average plant height, cut to soil level, and the aerial part was separated from the subterranean. The aerial and subterranean structures were separated, and evaluated as to their fresh and dry weights and foliar area. Generally, the larger sized tubers were more favorable to the development of subterranean parts. The number of emerged sprouts was not affected by tuber size; but plant height, foliar area and aerial part weights were larger when originated from large-sized tubers. The addition of sugar cane straw to the soil affected plants derivated from small tuber more than those originated from large one. The addition of growing quantities of straw caused reduction in the development of different kinds of subterranean structures, following for all variables one second-order equation. Application of growing quantities of straw caused a decrease in the sprouts number, area foliar and fresh and dry leaf and total aerial part weights. The formation and development of rhizomes and tubers + basal bulbs, similarly to the fresh and dry rhizome weights of tubers + basal bulbs were larger in September planting and the smallest in July. It was verified that, with exception of average plant height, the September planting generated a larger number of sprouts, foliar area and fresh and dry leaf and total aerial part weights. As for average plant height, the best results were observed at the May planting. For all analyzed variables, the July plantation always presented the smallest values. As to the time of evaluation, it was verified that all variables increased linearly in function of time.

A mudança do sistema de colheita da cana-de-açúcar com o uso de fogo para a colheita mecanizada, chamada de cana verde ou cana crua, é um processo irreversível, gradativo porém contínuo, previsto na legislação estadual paulista. Um dos pontos críticos no processo produtivo da cana-de-açúcar é a interferência imposta pelas plantas daninhas. A tiririca (Cyperus rotundus L.) é uma das plantas daninhas mais prejudiciais à cultura da cana-de-açúcar entretanto, com a implementação da colheita mecanizada, o desenvolvimento desta planta poderá ser afetado. O objetivo do experimento foi verificar se a adição de palha de cana-de-açúcar poderia afetar o desenvolvimento de plantas de tiririca provenientes de tubérculos de tamanho pequeno (0,22 a 0,34 g) e grande (1,01 a 1,14 g), plantados em diferentes épocas do ano. O experimento foi instalado em casa-de-vegetação, em Campinas, SP e disposto em blocos ao acaso com quatro repetições. Tubérculos dormentes de tiririca de tamanho pequeno e grande foram plantados em maio, julho e setembro e adicionou-se sobre o solo palha de cana-de-açúcar RB 855156, nas quantidades correspondentes a 0,0, 5,0, 10,0 e 15,0 t/ha. Em cada época de instalação do experimento, a cada 28 dias até 84 dias, as plantas de tiririca foram avaliadas quanto à altura média, cortadas ao nível do solo. As estruturas subterrâneas e aéreas foram separadas nas diferentes estruturas e avaliou-se suas biomassas frescas e secas e a área foliar. De modo geral, o tamanho maior dos tubérculos favoreceu o desenvolvimento da parte subterrânea. O número de brotações emergidas não foi afetado pelo tamanho de tubérculos mas, a altura média, área foliar e as biomassas das folhas e da parte aérea foram maiores quando originadas de tubérculos de tamanho grande. A adição de palha de cana-de-açúcar ao solo afetou mais as plantas originadas de tubérculos pequenos que de grandes. A adição de quantidades crescentes de palha de cana-de-açúcar causou redução no desenvolvimento nas diferentes estruturas subterrâneas, seguindo para todas as variáveis uma equação do segundo grau. Aplicações de quantidades crescentes de palha de cana-de-açúcar causaram diminuição no número de brotações, área foliar e as biomassas frescas e secas das folhas e da parte aérea total. A formação e o desenvolvimento dos rizomas e de tubérculos + bulbos basais, assim como das biomassas fresca e seca de rizomas e fresca de tubérculos + bulbos basais foram maiores no plantio realizados em setembro e menores nos de julho. Verificou-se que, exceto para altura média, o plantio de setembro proporcionou plantas com maior número de brotações, área foliar e biomassas fresca e seca de folhas e da parte aérea total. Para altura média, os melhores resultados foram observados no plantio de maio. Para todas as variáveis analisadas, o plantio de julho apresentou sempre os menores valores. Quanto ao efeito de época de avaliação, verificou-se que para todas as variáveis analisadas foram observados aumentos lineares em função do tempo. / The change in harvest systems for sugar cane from use of fire to mechanical harvest, is an irreversible process, gradual although continuous, foreseen in the São Paulo State Legislation. One of the critical points in the productive process of sugar cane is the interference imposed by weeds. Purple nutsedge (Cyperus rotundus L.) is one of the most sugar cane crop-damaging weeds, nevertheless with implementation of mechanical harvest technology, which leaves a thick layer straw superior to 20 ton/ha, the development of this plant could be affected. This experiment aimed to verify if the addition of sugar cane straw could affect the development of purple nutsedge plants deriving from tubers of small (0,20-0,34g) and large (1,01-1,14g) sizes, planted at different times of the year. The experiment was carrIED out in glass house, in Campinas, São Paulo, in randomized blocks with four replicates. Tubers of small and large sizes of purple nutsedge were planted in May, July and September, and RB 855156 sugar cane straw was added, in quantities corresponding to 0, 5,0, 10,0 and 15,0 ton/ha. Every 28 days until 84 days after each planting period, the purple nutsedge plants were evaluated as to their average plant height, cut to soil level, and the aerial part was separated from the subterranean. The aerial and subterranean structures were separated, and evaluated as to their fresh and dry weights and foliar area. Generally, the larger sized tubers were more favorable to the development of subterranean parts. The number of emerged sprouts was not affected by tuber size; but plant height, foliar area and aerial part weights were larger when originated from large-sized tubers. The addition of sugar cane straw to the soil affected plants derivated from small tuber more than those originated from large one. The addition of growing quantities of straw caused reduction in the development of different kinds of subterranean structures, following for all variables one second-order equation. Application of growing quantities of straw caused a decrease in the sprouts number, area foliar and fresh and dry leaf and total aerial part weights. The formation and development of rhizomes and tubers + basal bulbs, similarly to the fresh and dry rhizome weights of tubers + basal bulbs were larger in September planting and the smallest in July. It was verified that, with exception of average plant height, the September planting generated a larger number of sprouts, foliar area and fresh and dry leaf and total aerial part weights. As for average plant height, the best results were observed at the May planting. For all analyzed variables, the July plantation always presented the smallest values. As to the time of evaluation, it was verified that all variables increased linearly in function of time.