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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

Cocriação didática - o processo colaborativo de produção de material didático para curso semipresencial / Didactic co-creation - the collaborative process of didactic material production for blended learning course

Gabarrone, Melissa Rocha 24 August 2017 (has links)
O preparo e produção de material didático para cursos on-line demanda o trabalho de uma equipe multidisciplinar (com técnicos e especialistas de diferentes áreas) junto aos professores-autores. A organização e o processo de produção são complexos e específicos para cada projeto e instituição de ensino. Nesta pesquisa, o objetivo é analisar como ocorreu o processo colaborativo de produção de material didático no curso semipresencial de Licenciatura em Ciências da USP/UNIVESP (no período de 2013). Este estudo é definido como pesquisa qualitativa, identificada como estudo de caso. Para alcançar os objetivos previstos foi realizada coleta de dados documentais e entrevistas com profissionais que participaram do processo de criação. Para análise dos dados obtidos foi realizado inicialmente o levantamento de posicionamentos teóricos ligados aos principais termos que permeiam o estudo. Por meio de análise de conteúdo foram estabelecidas as relações entre a teoria estudada e os dados coletados. Ao final, conclui-se que, por se tratar de um trabalho em equipe, o conteúdo (texto) original precisa passar por alterações e adequações ao longo de todo o processo até se tornar material didático do curso (em seus diferentes formatos). Vale destacar que esse processo de produção é acompanhado, discutido e validado pelo professor em todas as suas etapas. Como em toda equipe multidisciplinar, a comunicação e as inter-relações entre os profissionais são constantes e significativas para o processo. É nesse contexto de trabalho em equipe (junto ao professorautor) que ocorrem momentos de cocriação didática, resultado de um trabalho colaborativo e de criação. Verificou-se que esse processo de atuação das equipes na produção de material didático tem momentos intercalados de trabalho individualizado e colaborativo. Apesar desse fluxo estar desenhado de forma linear, com etapas predefinidas, é nas inter-relações e trocas de conhecimentos, sugestões e adequações do conteúdo que ocorrem momentos de cocriação. Trata-se de um processo híbrido: linear e de cocriação. Linear em sua estrutura e forma de trabalho e de cocriação como parte natural das relações humanas e da produção coletiva de conhecimento. / The preparation and the production of didactic materials for on-line courses demand a multidisciplinary teamwork (technicians and specialists from different areas) along with teacherauthors. The organization and the process of production are complex and specific for each project and teaching institution. The objective of this research is to analyze how the collaborative process of the didactic material produced in the blended learning course of the Licentiate Degree in Science from USP/UNIVESP (conducted over a certain period of time. Qualitative approach has been adopted for this study, more specifically, a case study research. In order to accomplish our goals, we have conducted data collection by using review of documents as well as interviews with the professionals that participated in the creation process. For the purpose of analyzing the collected data, we have gathered diverse theoretical approaches related to the main topics of this study. By having the content analyzed, a relationship between the studied theory and the data collected has been established. In conclusion, due to the fact that it is the product of a teamwork, the original content (text) needs to go through some changes and adaptations during the whole process until the final version of the didactic material (in all its different formats) is completed. It is important to emphasize that every step during the process of production is attended, discussed and validated by the teacher-author. As in any multidisciplinary teamwork, the communication and the interrelationships are constant and meaningful to the process. It is in this context of teamwork (including the teacher-author) that the didactic co-creation occurs, being the result of collaborative and creation work. It has been verified that the groups worked in intercalated moments throughout the production of the material: as individuals or in collaboration. Despite the fact that there are predefined steps which are supposed to flow in a linear way, co-creation takes place during the interrelationships, the exchange of knowledge and suggestions and the adaptation of the content. It turns out to be a hybrid process: linear and co-creative. Linear in its structure and its way of working and co-creative as a natural part of human relationships and as a collective production of knowledge.
12

O uso variável do pronome de segunda pessoa você(s)/cês(s) na cidade de São Paulo / The variable use of the second person pronoun você(s)/cê(s) in the city of São Paulo

Nascimento, Ivanete Belem do 17 February 2011 (has links)
Esta dissertação trata da variação no uso do pronome de segunda pessoa (singular e plural) na cidade de São Paulo. Duas formas alternativas são empregadas nessa comunidade de fala: a variante plena você(s) e a forma foneticamente reduzida cê(s). A pesquisa é desenvolvida de acordo com os pressupostos teórico-metodológicos da Sociolinguística Variacionista. Foram desenvolvidas análises sincrônica e diacrônica com dados extraídos de duas amostras (NURC-SP-1970 e GESOL-SP-2000). Os resultados evidenciam um equilíbrio na distribuição das duas variantes nessa comunidade de fala. Embora se verifique uma típica mudança em progresso (tempo aparente) na década de 1970, a análise dos anos 2000 revela um caso de variação estável na comunidade de fala. Nos anos 2000, a variante inovadora, a forma reduzida cê, tende a ser favorecida pelos informantes mais jovens e pelas mulheres acima de 50 anos de idade. Cê é desfavorecida entre os indivíduos de uma faixa etária intermediária (entre 35 e 45 anos) o que pode estar relacionado a questões de monitoramento da fala e ao mercado linguístico (Paiva & Duarte, 2003). Adicionalmente, cê tende a ser evitado entre os indivíduos mais escolarizados; e é favorecido em interações cujos informantes são familiares ou amigos. De um ponto de vista linguístico, o emprego das variantes é correlacionado pelo Princípio do Contorno Obrigatório e por questões morfossintáticas, semânticas e discursivo-cognitivas, com especial atenção à referência do pronome. Os resultados corroboram a hipótese de cliticização da variante reduzida, mas fornecem um contra-argumento à correlação direta entre erosão fonética e abstratização semântica, defendida na literatura sobre gramaticalização. / This master thesis analyzes the variation in the use of the second person pronoun (singular and plural) in the city of São Paulo. Two alternative forms are employed: você you, and a phonetically reduced form cê you. The research is developed according to the theoretical and methodological framework of Variationist Sociolinguistics. Both synchronic and diachronic multivariate analyses are pursued, with data extracted from two samples (NURC-SP-1970 and GESOL-SP-2000). The results show a balance in the distribution of the two variants in the speech community. Although it was observed a typical change in progress (apparent time) in the 1970s, the analysis of the 2000s data reveals a case of stable variation in the speech community. In the 2000s, the innovative, phonetically reduced variant tends to be favored by younger people and women over 50 years old. Cê is disfavored among individuals between 35 and 45 years which can be related to issues of speech monitoring and the linguistic market (Paiva & Duarte, 2003). In addition, cê tends to be avoided by those whose level of education is higher, and is favored in conversations between informants who are friends or relatives. From a linguistic perspective, the use of variants is correlated by the Obligatory Contour Principle (OCP) and by morphosyntactic, semantic and discursive-cognitive factors, with special attention to the reference of the pronoun. Results confirm the hypothesis of cliticization of the reduced variant, but reveal a counterargument for the direct correlation between \"phonetic erosion\" and \"semantic abstraction\", which has been claimed in the literature on grammaticalization
13

O uso variável do pronome de segunda pessoa você(s)/cês(s) na cidade de São Paulo / The variable use of the second person pronoun você(s)/cê(s) in the city of São Paulo

Ivanete Belem do Nascimento 17 February 2011 (has links)
Esta dissertação trata da variação no uso do pronome de segunda pessoa (singular e plural) na cidade de São Paulo. Duas formas alternativas são empregadas nessa comunidade de fala: a variante plena você(s) e a forma foneticamente reduzida cê(s). A pesquisa é desenvolvida de acordo com os pressupostos teórico-metodológicos da Sociolinguística Variacionista. Foram desenvolvidas análises sincrônica e diacrônica com dados extraídos de duas amostras (NURC-SP-1970 e GESOL-SP-2000). Os resultados evidenciam um equilíbrio na distribuição das duas variantes nessa comunidade de fala. Embora se verifique uma típica mudança em progresso (tempo aparente) na década de 1970, a análise dos anos 2000 revela um caso de variação estável na comunidade de fala. Nos anos 2000, a variante inovadora, a forma reduzida cê, tende a ser favorecida pelos informantes mais jovens e pelas mulheres acima de 50 anos de idade. Cê é desfavorecida entre os indivíduos de uma faixa etária intermediária (entre 35 e 45 anos) o que pode estar relacionado a questões de monitoramento da fala e ao mercado linguístico (Paiva & Duarte, 2003). Adicionalmente, cê tende a ser evitado entre os indivíduos mais escolarizados; e é favorecido em interações cujos informantes são familiares ou amigos. De um ponto de vista linguístico, o emprego das variantes é correlacionado pelo Princípio do Contorno Obrigatório e por questões morfossintáticas, semânticas e discursivo-cognitivas, com especial atenção à referência do pronome. Os resultados corroboram a hipótese de cliticização da variante reduzida, mas fornecem um contra-argumento à correlação direta entre erosão fonética e abstratização semântica, defendida na literatura sobre gramaticalização. / This master thesis analyzes the variation in the use of the second person pronoun (singular and plural) in the city of São Paulo. Two alternative forms are employed: você you, and a phonetically reduced form cê you. The research is developed according to the theoretical and methodological framework of Variationist Sociolinguistics. Both synchronic and diachronic multivariate analyses are pursued, with data extracted from two samples (NURC-SP-1970 and GESOL-SP-2000). The results show a balance in the distribution of the two variants in the speech community. Although it was observed a typical change in progress (apparent time) in the 1970s, the analysis of the 2000s data reveals a case of stable variation in the speech community. In the 2000s, the innovative, phonetically reduced variant tends to be favored by younger people and women over 50 years old. Cê is disfavored among individuals between 35 and 45 years which can be related to issues of speech monitoring and the linguistic market (Paiva & Duarte, 2003). In addition, cê tends to be avoided by those whose level of education is higher, and is favored in conversations between informants who are friends or relatives. From a linguistic perspective, the use of variants is correlated by the Obligatory Contour Principle (OCP) and by morphosyntactic, semantic and discursive-cognitive factors, with special attention to the reference of the pronoun. Results confirm the hypothesis of cliticization of the reduced variant, but reveal a counterargument for the direct correlation between \"phonetic erosion\" and \"semantic abstraction\", which has been claimed in the literature on grammaticalization
14

In vitro and in vivo characterisation of the OCP-related photoprotective mechanism in the cyanobacterium Synechocystis PCC6803

Gwizdala, Michal 16 November 2012 (has links) (PDF)
Strong light can cause damage and be lethal for photosynthetic organisms. An increase of thermal dissipation of excess absorbed energy at the level of photosynthetic antenna is one of the processes protecting against deleterious effects of light. In cyanobacteria, a soluble photoactive carotenoid binding protein, Orange Carotenoid Protein (OCP) mediates this process. The photoactivated OCP by interacting with the core of phycobilisome (PB; the major photosynthetic antenna of cyanobacteria) triggers the photoprotective mechanism, which decreases the energy arriving at the reaction centres and PSII fluorescence. The excess energy is dissipated as harmless heat. To regain full PB capacity in low light intensities, theFluorescence Recovery Protein (FRP) is required. FRP accelerates the deactivation of OCP.In this work, I present my input in the understanding of the mechanism underlying the OCPrelated photoprotection. I further characterized the FRP of Synechocystis PCC6803, the model organism in our studies. I established that the Synechocystis FRP is shorter than what it was proposed in Cyanobase and it begins at Met26. Our results also revealed the great importance of a high OCP to FRP ratio for existence of photoprotection. The most remarkable achievement of this thesis is the in vitro reconstitution of the OCPrelated mechanism using isolated OCP, PB and FRP. I demonstrated that light is only needed for OCP photoactivation but OCP binding to PB is light independent. Only the photoactivated OCP is able to bind the PB and quench all its fluorescence. Based on our in vitro experiments we proposed a molecular model of OCP-related photoprotection. The in vitro reconstituted system was applied to examine the importance of a conserved salt bridge (Arg155-Glu244) between the two domains of OCP and showed that this salt bridge stabilises the inactive form of OCP. During photoactivation this salt bridge is broken and Arg155 is involved in the interaction between the OCP and the PB. The site of OCP binding in the core of a PB wasalso investigated with the in vitro reconstituted system. Our results demonstrated that the terminal energy emitters of the PB are not needed and that the first site of fluorescence quenching is an APC trimer emitting at 660 nm. Finally, we characterised the properties of excited states of the carotenoid in the photoactivated OCP showing that one of these states presents a very pronounced charge transfer character that likely has a principal role in energy dissipation. Our results strongly suggested that the OCP not only induces thermal energy dissipation but also acts as the energy dissipator.
15

Comparative studies of electrochemical corrosion behaviour of mild steel in some agro-fluids

Ogazi, Anthony Chikere 04 1900 (has links)
The electrochemical corrosion behavior of mild steel in apple, grape, mango, orange and the mixture of these juices was investigated in this study. Open circuit potential and weight loss analytical techniques were employed to establish the comparative corrosion rates of this material in the agro media over the interval of five days for a sixty-day immersion period at an ambient temperature. The chemical compositions of both mild steel and the agro media were determined to ascertain corrosion mechanism for the reaction. Polarization behavior of mild steel in the agro media were determined by Tafel extrapolation curves. The analysis showed that cathodic polarization curves were almost identical irrespective of the concentration of the various media while the anodic polarization curves exhibited varying active and passive corrosion behaviour due to passivating oxide films. The analysis of the results further showed that the corrosion rate of the metallic sample decreased with longer immersion periods which could be attributed to a gradual decline in acidity of these media as revealed by the pH results. Hence, the evolution of hydrogen gas and reduction of dissolved oxygen molecules from the reacting system were presumed to be major factors retarding corrosion of the solution involved. Similarly, the presence of suspended particles on the surface of the test steel sample could have also led to the impediment to corrosion rates from the surrounding atmosphere. Microscopic analysis of the corroded mild steel specimens revealed uniform and localized corrosion with Fe2O3 spotted as the main corrosion product. The result obtained from the electrochemical study showed that the corrosion rate of the mild steel sample was highest in the orange medium (1.53mm/yr), followed by grape medium (1.40mm/yr), mixture of these media (0.67mm/yr), mango medium (0.40mm/yr) while the metal corroded least in the apple medium (0.30mm/yr) over the duration of immersion. / Engineering : Chemical / M. Tech. (Chemical Engineering)
16

Cocriação didática - o processo colaborativo de produção de material didático para curso semipresencial / Didactic co-creation - the collaborative process of didactic material production for blended learning course

Melissa Rocha Gabarrone 24 August 2017 (has links)
O preparo e produção de material didático para cursos on-line demanda o trabalho de uma equipe multidisciplinar (com técnicos e especialistas de diferentes áreas) junto aos professores-autores. A organização e o processo de produção são complexos e específicos para cada projeto e instituição de ensino. Nesta pesquisa, o objetivo é analisar como ocorreu o processo colaborativo de produção de material didático no curso semipresencial de Licenciatura em Ciências da USP/UNIVESP (no período de 2013). Este estudo é definido como pesquisa qualitativa, identificada como estudo de caso. Para alcançar os objetivos previstos foi realizada coleta de dados documentais e entrevistas com profissionais que participaram do processo de criação. Para análise dos dados obtidos foi realizado inicialmente o levantamento de posicionamentos teóricos ligados aos principais termos que permeiam o estudo. Por meio de análise de conteúdo foram estabelecidas as relações entre a teoria estudada e os dados coletados. Ao final, conclui-se que, por se tratar de um trabalho em equipe, o conteúdo (texto) original precisa passar por alterações e adequações ao longo de todo o processo até se tornar material didático do curso (em seus diferentes formatos). Vale destacar que esse processo de produção é acompanhado, discutido e validado pelo professor em todas as suas etapas. Como em toda equipe multidisciplinar, a comunicação e as inter-relações entre os profissionais são constantes e significativas para o processo. É nesse contexto de trabalho em equipe (junto ao professorautor) que ocorrem momentos de cocriação didática, resultado de um trabalho colaborativo e de criação. Verificou-se que esse processo de atuação das equipes na produção de material didático tem momentos intercalados de trabalho individualizado e colaborativo. Apesar desse fluxo estar desenhado de forma linear, com etapas predefinidas, é nas inter-relações e trocas de conhecimentos, sugestões e adequações do conteúdo que ocorrem momentos de cocriação. Trata-se de um processo híbrido: linear e de cocriação. Linear em sua estrutura e forma de trabalho e de cocriação como parte natural das relações humanas e da produção coletiva de conhecimento. / The preparation and the production of didactic materials for on-line courses demand a multidisciplinary teamwork (technicians and specialists from different areas) along with teacherauthors. The organization and the process of production are complex and specific for each project and teaching institution. The objective of this research is to analyze how the collaborative process of the didactic material produced in the blended learning course of the Licentiate Degree in Science from USP/UNIVESP (conducted over a certain period of time. Qualitative approach has been adopted for this study, more specifically, a case study research. In order to accomplish our goals, we have conducted data collection by using review of documents as well as interviews with the professionals that participated in the creation process. For the purpose of analyzing the collected data, we have gathered diverse theoretical approaches related to the main topics of this study. By having the content analyzed, a relationship between the studied theory and the data collected has been established. In conclusion, due to the fact that it is the product of a teamwork, the original content (text) needs to go through some changes and adaptations during the whole process until the final version of the didactic material (in all its different formats) is completed. It is important to emphasize that every step during the process of production is attended, discussed and validated by the teacher-author. As in any multidisciplinary teamwork, the communication and the interrelationships are constant and meaningful to the process. It is in this context of teamwork (including the teacher-author) that the didactic co-creation occurs, being the result of collaborative and creation work. It has been verified that the groups worked in intercalated moments throughout the production of the material: as individuals or in collaboration. Despite the fact that there are predefined steps which are supposed to flow in a linear way, co-creation takes place during the interrelationships, the exchange of knowledge and suggestions and the adaptation of the content. It turns out to be a hybrid process: linear and co-creative. Linear in its structure and its way of working and co-creative as a natural part of human relationships and as a collective production of knowledge.
17

Distribuce organochlorových pesticidů do životního prostředí / Distribution of organochlorine pesticides into the environment

Plačková, Lucie January 2011 (has links)
The master’s thesis was focused on the determination of persistent organic pollutants, namely polychlorinated biphenyls were detected (PCB 28, 52, 101, 118, 138, 153 and 180) and organochlorine pesticides. A total of 31 were evaluated contamination of organochlorine compounds. The level of contamination by organic pollutants were detected in 20 samples, 9 samples were sediments from the river Jevišovka, which is located in the South Moravia and 11 samples represented arable soil samples and were collected in the vicinity of this river. In the theoretical part are characterized both groups of substances, their fate in the environment and negative effects on living organisms. The experimental part focuses on the sampling procedures and processing for analysis by gas chromatography. There is described a complex analytical process involving the three extraction methods (accelerated solvent extraction, microwave extraction and ultrasonic extraction), extract purification and self-determination by gas chromatography with electron capture detector (GC / ECD). In the samples of sediment were detected these PCB congeners (138, 153 and 180) and organochlorine pesticides (b-HCH, HCB, p,p’-DDE, p,p’-DDD, p,p’-DDT and o,p’-DDT).. In soil samples were detected PCB congener 28 and organochlorine pesticides (HCB, b-HCH, heptachlor, aldrin, dieldrin, p,p’-DDE, p,p’-DDD, p,p’-DDT and o,p’-DDT).
18

Corrosion Behavior of HVAF-Sprayed Bi-Layer Coatings

Sadeghimeresht, Esmaeil January 2016 (has links)
In a variety of engineering applications, components are subjected to corrosive environment. Protective coatings are essential to improve the functional performances and/or extend the lifetime of the components. Thermal sprayingas a cost-effective coating deposition technique offers high flexibility in coatings' chemistry/morphology/microstructure design. However, the inherent pores formed during spraying limit the use of coatings for corrosion protection. The recently developed supersonic spray method, High-Velocity-Air-Fuel (HVAF), brings significant advantages in terms of cost and coating properties. Although severely reduced, the pores are not completely eliminated even with the HVAF process. In view of the above gap to have a high quality coating, bi-layer coatings have been developed to improve the corrosion resistance of the coatings. In a bi-layer coating, an intermediate layer is deposited on the substrate before spraying the coating. The electrochemical behavior of each layer is important to ensure a good corrosion protection. The corrosion behavior of the layers strongly depends on coating composition and microstructure, which are affected by feedstock material and spraying process. Therefore, the objective of the researchis to explore the relationships between feedstock material, spraying process, microstructure and corrosion behavior of bi-layer coatings. A specific motivationis to understand the corrosion mechanisms of the intermediate layer which forms the basis for developing superior protective coatings. Cr3C2-NiCr top layer and intermediate layers (Fe-, Co- and Ni-based) were sprayed by different thermal spraying processes. Microstructure analysis, as well as various corrosion tests, e.g., electrochemical, salt spray and immersion tests were performed. The results showed a direct link between the corrosion potential (Ecorr) of the intermediate layer and the corrosion mechanisms. It was found that the higher corrosion resistance of Ni-based coatings than Fe- and Co-based coatings was due to higher Ecorr of the coating in the galvanic couple with top layers. Inter-lamellar boundaries and interconnected pores reduced the corrosion resistance of intermediate layers, however a sufficient reservoir of protective scale-forming elements (such as Cr or Al) improved the corrosion behavior.
19

Persistentní organické polutanty v životním prostředí Jihomoravského kraje / Persistent Organic Pollutants in the Environment of the Southern Moravia Region

Lána, Radim January 2008 (has links)
Byla provedena studie bioakumulace POP v potravním řetězci a historických trendů hladin POP v sedimentech Brněnské přehrady. Současné úrovně kontaminantů byly stanoveny v jelci tloušti z řeky Svratky a rovněž byla posouzena hygienická kvalita ryb z rekreačního rybolovu. Současné úrovně POP v terestrickém ekosystému byly posouzeny pomocí analýz jehličí jako přirozeného indikátoru znečištění. Studium jedinců tří druhů dravých ptáků z ČR bylo zaměřeno na zhodnocení kontaminace těchto vrcholných predátorů POP. S pomocí analýz vybraných druhů ryb ze Záhlinických rybníků byl rovněž studován proces bioakumulace. Posouzení parametrů dvou moderních extrakčních technik a jejich využitelnosti pro izolaci POP z různých matric bylo námětem poslední části dizertace.
20

Intraocular lenses with surfaces functionalized by biomolecules in relation with lens epithelial cell adhesion / Fonctionnalisation de lentilles intraoculaires acryliques par greffage de biomolécules limitant la cataracte secondaire

Huang, Yi-Shiang 08 December 2014 (has links)
L’Opacification Capsulaire Postérieure (OCP) est la fibrose de la capsule développée sur la lentille intraoculaire implantée (LIO) suite à la dé-différenciation de cellules épithéliales cristalliniennes (LECs) subissant une transition épithélio-mésenchymateuse (EMT). La littérature a montré que l'incidence de l’OCP est multifactorielle, dont l'âge ou la maladie du patient, la technique de chirurgie, le design et le matériau de la LIO. La comparaison des LIOs en acryliques hydrophiles et hydrophobes montre que les premières ont une OCP plus sévère, médiée par la transition EMT. En outre, il est également démontré que l'adhérence des LECs est favorisée sur des matériaux hydrophobes par rapport à ceux hydrophiles. Une stratégie biomimétique destinée à promouvoir l’adhérence des LECs sans dé-différenciation en vue de réduire le risque de développement de l’OCP est proposée. Dans cette étude, les peptides RGD, ainsi que les méthodes de greffage et de quantification sur un polymère acrylique hydrophile ont été étudiés. La surface fonctionnalisée des LIOs favorisant l'adhérence des LECs via les récepteurs de type intégrine peut être utilisée pour reconstituer la structure capsule-LEC-LIO en sandwich, ce qui est considéré dans la littérature comme un moyen de limiter la formation de l‘OCP. Les résultats montrent que le biomatériau innovant améliore l'adhérence des LEC, et présente également les propriétés optiques (transmission de la lumière , banc optique) similaires et mécaniques (force haptique de compression, force d'injection de la LIO) comparables à la matière de départ. En outre, par rapport au matériau hydrophobe IOL, ce biomatériau bioactif présente des capacités similaires vis à vis de l’adhérence des LECs, le maintien de la morphologie, et l'expression de biomarqueurs de l’EMT. Les essais in vitro suggèrent que ce biomatériau a le potentiel de réduire certains facteurs de risque de développement de l’OCP. / Posterior Capsular Opacification (PCO) is the capsule fibrosis developed onto the implanted IntraOcular Lens (IOL) by the de-differentiation of Lens Epithelial Cells (LEC) undergoing Epithelial-Mesenchymal Transition (EMT). Literature has shown that the incidence of PCO is multifactorial including patient’s age or disease, surgical technique, and IOL design and material. Reports comparing hydrophilic and hydrophobic acrylic IOLs show the former has more severe PCO after EMT transition. Additionally, the LEC adhesion is favored onto the hydrophobic materials compared to the hydrophilic ones. A biomimetic strategy to promote LEC adhesion without de-differentiation to reduce PCO development risk is proposed. RGD peptides, as well as their grafting and quantification methods on a hydrophilic acrylic polymer were investigated. The surface functionalized IOL promoting LEC adhesion via integrin receptors can be used to reconstitute the capsule-LEC-IOL sandwich structure, which is considered to prevent PCO formation in literature. The results show the innovative biomaterial improves LEC adhesion, and also exhibits similar optical (light transmittance, optical bench) and mechanical (haptic compression force, IOL injection force) properties comparing to the starting material. In addition, comparing to the hydrophobic IOL material, this bioactive biomaterial exhibits similar abilities in LEC adhesion, morphology maintenance, and EMT biomarker expression. The in vitro assays suggest this biomaterial has the potential to reduce some risk factors of PCO development.

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