Is it possible that by planning in a more sustainable way when building a new area, we can improve our environment? Most environmental problems are created in cities and therefore they should hold the solution. Through a more ecological urban planning a better foundation for sustainable housing could be created. This report follows the process in coming up with a proposal for a master plan for an area in Odense, Denmark. By going through principles for sustainable urban planning made by different organizations and persons and from these extract main principles, a background is founded to continue the proposal from. Denmark works a lot on expanding their green areas, maintain the existing ones and recreate what’s being destroyed as cities expand. The municipality of Odense works along these outlines and has a special interest in preserving old neighbourhoods and buildings that have an important local history. The area Gartnerskolen – Søhus is located in the outskirts of Odense and is currently used for cultivating fruits and as a university for gardeners. The surroundings are well known for the history of an area with many greenhouses and this heritage is important to preserve. During the planning process it’s been important to create a pleasant and well functioning area – not only a sustainable experiment. Gartnerskolen – Søhus should be an attractive place to live which is also sustainable. There are several buildings on site and all of them have been preserved but in some cases the purpose of them has been altered. Likewise the tall hedges that originally protect the fruit trees from hard winds have been maintained to add more character to the area. In order to facilitate the understanding those parts of this report that concern the master plan it is recommended to have the appendix with the proposal at hand.

<p>Is it possible that by planning in a more sustainable way when building a new area, we can improve our environment?</p><p>Most environmental problems are created in cities and therefore they should hold the solution. Through a more ecological urban planning a better foundation for sustainable housing could be created.</p><p>This report follows the process in coming up with a proposal for a master plan for an area in Odense, Denmark. By going through principles for sustainable urban planning made by different organizations and persons and from these extract main principles, a background is founded to continue the proposal from.</p><p>Denmark works a lot on expanding their green areas, maintain the existing ones and recreate what’s being destroyed as cities expand. The municipality of Odense works along these outlines and has a special interest in preserving old neighbourhoods and buildings that have an important local history.</p><p>The area Gartnerskolen – Søhus is located in the outskirts of Odense and is currently used for cultivating fruits and as a university for gardeners. The surroundings are well known for the history of an area with many greenhouses and this heritage is important to preserve.</p><p>During the planning process it’s been important to create a pleasant and well functioning area – not only a sustainable experiment. Gartnerskolen – Søhus should be an attractive place to live which is also sustainable. There are several buildings on site and all of them have been preserved but in some cases the purpose of them has been altered. Likewise the tall hedges that originally protect the fruit trees from hard winds have been maintained to add more character to the area.</p><p>In order to facilitate the understanding those parts of this report that concern the master plan it is recommended to have the appendix with the proposal at hand.</p>

Glucose is a monosaccharide and fuel for body, it cannot pass through membrane by simple diffusion so, integral transmembrane proteins named glucose transporters (Gluts) are involved in the regulation of the movement of glucose between the extracellular and intracellular spaces within the body. GLUT1 and GLUT3 have previously been shown by cold detergent extraction methods to reside in distinct plasma membrane domains in non-polarized mammalian cells, with GLUT1, but not GLUT3, residing  in detergent-resistant membrane (DRM) domains. To confirm this observation under less invasive conditions, molecular fusion tags are inserted in the first external loop in Glut1 with biotin ligase acceptor peptide (BLAP) between Ser-55 and Ile-56 and in Glut3 with Acyl carrier peptide (ACP) in between Val-57 and Leu-58 respectively. These Glut fusion proteins will be used in order to confirm these observations by fluorescence recovery after photobleaching (FRAP) and single molecule fluorescence microscopy in live cells. hGLUT1-EGFP, hGLUT1 (AgeI)-EGFP recombinants were constructed and transfected human embryonic kidney cells (HEK-293) quantum dot images supports the fact that EGFP transfected cells uniformly and is distributed in the cell cytoplasm, hGLUT1-EGFP transfected cells and is localized to the cell membrane and hGLUT1 (AgeI)-EGFP transfected cells and located to the plasma membrane with high intensity.

<p>Glucose is a monosaccharide and fuel for body, it cannot pass through membrane by simple diffusion so, integral transmembrane proteins named glucose transporters (Gluts) are involved in the regulation of the movement of glucose between the extracellular and intracellular spaces within the body. GLUT1 and GLUT3 have previously been shown by cold detergent extraction methods to reside in distinct plasma membrane domains in non-polarized mammalian cells, with GLUT1, but not GLUT3, residing  in detergent-resistant membrane (DRM) domains. To confirm this observation under less invasive conditions, molecular fusion tags are inserted in the first external loop in Glut1 with biotin ligase acceptor peptide (BLAP) between Ser-55 and Ile-56 and in Glut3 with Acyl carrier peptide (ACP) in between Val-57 and Leu-58 respectively. These Glut fusion proteins will be used in order to confirm these observations by fluorescence recovery after photobleaching (FRAP) and single molecule fluorescence microscopy in live cells. hGLUT1-EGFP, hGLUT1 (<em>AgeI</em>)-EGFP recombinants were constructed and transfected human embryonic kidney cells (HEK-293) quantum dot images supports the fact that EGFP transfected cells uniformly and is distributed in the cell cytoplasm, hGLUT1-EGFP transfected cells and is localized to the cell membrane and hGLUT1 (<em>AgeI</em>)-EGFP transfected cells and located to the plasma membrane with high intensity.</p>

Thesis (Ph. D.)--Stockholms högskola, 1930. / Summary in German. Includes bibliographical references (p. 221-226).