Global ETD Search

11	Probing the interactions between component B and the hydroxylase of methane monooxygenase / Wallar, Bradley James. January 2000 (has links) Thesis (Ph.D.)--University of Minnesota, 2000. / Includes bibliographical references. Also available on the World Wide Web as a PDF file.
12	Active site studies and design of ligands for affinity column separation of 2,5-dihydroxyacetanilide epoxidase (DHAE) I and II Allen, Scott E. 03 September 2002 (has links) A series of compounds, 7-8 and 20-25, were tested as competitive inhibitors of 2,5-dihydroxyacetanilide epoxidase I (DHAE I) and DHAE II. A Hammett plot was constructed for each enzyme to determine the effect of electron density on inhibition. DHAE I gave a linear, highly correlated plot (r²=0.91) that signifies the importance of the amide oxygen in 1 on substrate binding. The plot for DHAE 11 is curved showing the greatest degree of inhibition with 7 suggesting steric factors within the active site control substrate binding. From these data, we conclude that each enzyme binds substrate in an opposite fashion and that this alone controls the stereochemistry of epoxide formation in 2 and 3. Alternative substrates, 26-29 and 33, were also synthesized and tested for product formation. All compounds, except 29, were accepted as alternative substrates, although the rates varied significantly. Surprisingly, 33 was accepted as an alternative substrate of DHAE II suggesting that the conformation of the amide bond in 33 is similar to the conformation required for catalytic activity in this enzyme. This information was then used to design ligands for affinity column separation of DHAE I and DHAE II from their protein mixtures. 35 and 36 were synthesized and attached to carbonyl di-imidazole activated agarose. Column I was tested three times with DHAE I enzyme preparations. The first attempt did not result in active enzyme being eluted from the column. The second attempt maintained the resin in the oxidized state. Protein was found to elute very quickly: no protein was found after fraction 4. The third attempt resulted in active enzyme in fractions 4-23. Column 2 was used twice for the attempted isolation of DHAE II from its protein mixture. The second attempt for column 2 mirrored the results for the third attempt with column 1. Neither column resulted in homogeneous enzyme by SDS-PAGE. / Graduation date: 2003 Ligand binding (Biochemistry) Oxygenases -- Separation
13	Mechanistic studies on non-heme monooxygenases : dopamine [beta]-monooxygenase and pseudomonas oleovorans monooxygenase system Wimalasena, Kandatege 08 1900 (has links) No description available. Oxygenases Oxygen Metabolism Chemistry, Organic
14	Multiphase biochemistry : Liquid membranes and the affinity chromatography of oxygenases Landgraff, Laura Mastrogianni 05 1900 (has links) No description available. Membranes (Technology) Affinity chromatography Oxygenases
15	Transition state studies on protocatechuate 3,4-dioxygenase Kaighobadi, Joni N. 12 1900 (has links) No description available. Oxidases Oxygenases Oxidation-reduction reaction
16	Isolation of phenylalanine hydroxylases and enzymatic studies with 3- and 4- pyridylalanine Han, Jin Hee 05 1900 (has links) No description available. Enzymatic analysis Chemistry, Analytic Oxygenases
17	A kinetic and resonance Raman spectroscopic investigation of protocatechuate-3,4-dioxygenase Phillips, Robert Stephen 08 1900 (has links) No description available. Oxygenases Raman spectroscopy Enzymatic analysis
18	Interplay between 2-oxoglutarate oxygenases and cancer : studies on the aspartyl/asparaginyl-β-hydroxylase Pfeffer, Inga January 2014 (has links) No description available.
19	Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish / Binder, Robert L. January 1981 (has links) Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1981. / Supervised by John J. Stegeman. Vita. Includes bibliographical references (leaves 239-262).
20	CRYSTALLIZATION AND CHARACTERIZATION OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE FROM HIGHER PLANTS Johal, Sarjit Singh January 1980 (has links) No description available. Plant proteins. Oxygenases. Crystallization. Botanical chemistry.

Search results